WEIZZ: Automatic grey-box fuzzing for structured binary formats

Fuzzing technologies have evolved at a fast pace in recent years, revealing bugs in programs with ever increasing depth and speed. Applications working with complex formats are however more difficult to take on, as inputs need to meet certain format-specific characteristics to get through the initial parsing stage and reach deeper behaviors of the program. Unlike prior proposals based on manually written format specifications, we propose a technique to automatically generate and mutate inputs for unknown chunk-based binary formats. We identify dependencies between input bytes and comparison instructions, and use them to assign tags that characterize the processing logic of the program. Tags become the building block for structure-aware mutations involving chunks and fields of the input. Our technique can perform comparably to structure-aware fuzzing proposals that require human assistance. Our prototype implementation WEIZZ revealed 16 unknown bugs in widely used programs

Thyroid-specific transcription factors control Hex promoter activity

The homeobox-containing gene Hex is expressed in several cell types, including thyroid follicular cells, in which it regulates the transcription of tissue-specific genes. In this study the regulation of Hex promoter activity was investigated. Using co-transfection experiments, we demonstrated that the transcriptional activity of the Hex gene promoter in rat thyroid FRTL-5 cells is ∼10-fold greater than that observed in HeLa and NIH 3T3 cell lines (which do not normally express the Hex gene). To identify the molecular mechanisms underlying these differences, we evaluated the effect of the thyroid-specific transcription factor TTF-1 on the Hex promoter activity. TTF-1 produced 3-4-fold increases in the Hex promoter activity. Gel-retardation assays and mutagenesis experiments revealed the presence of functionally relevant TTF-1 binding sites in the Hex promoter region. These in vitro data may also have functional relevance in vivo, since a positive correlation between TTF-1 and Hex mRNAs was demonstrated in human thyroid tissues by means of RT-PCR analysis. The TTF-1 effect, however, is not sufficient to explain the difference in Hex promoter activity between FRTL-5 and cells that do not express the Hex gene. For this reason, we tested whether Hex protein is able to activate the Hex promoter. Indeed, co-transfection experiments indicate that Hex protein is able to increase the activity of its own promoter in HeLa cells ∼4-fold. TTF-1 and Hex effects are additive: when transfected together in HeLa cells, the Hex promoter activity is increased 6-7-fold. Thus, the contemporary presence of both TTF-1 and Hex could be sufficient to explain the higher transcriptional activity of the Hex promoter in thyroid cells with respect to cell lines that do not express the Hex gene. These findings demonstrate the existence of direct cross-regulation between thyroid-specific transcription factors

UVES/VLT high resolution absorption spectroscopy of the GRB080330 afterglow: a study of the GRB host galaxy and intervening absorbers

We study the Gamma Ray Burst (GRB) environment and intervening absorbers by analyzing the optical absorption features produced by gas surrounding the GRB or along its line of sight. We analyzed high resolution spectroscopic observations (R=40000, S/N=3 - 6) of the optical afterglow of GRB080330, taken with UVES at the VLT ~ 1.5 hours after the GRB trigger. The spectrum illustrates the complexity of the ISM of the GRB host galaxy at z = 1.51 which has at least four components in the main absorption system. We detect strong FeII, SiII, and NiII excited absorption lines associated with the bluemost component only. In addition to the host galaxy, at least two more absorbers lying along the line of sight to the afterglow have been detected in the redshift range 0.8 < z < 1.1, each exhibiting MgII absorption. For the bluemost component in the host galaxy, we derive information about its distance from the site of the GRB explosion. We do so by assuming that the excited absorption lines are produced by indirect UV pumping, and compare the data with a time dependent photo-excitation code. The distance of this component is found to be 280+40-50 pc, which is lower than found for other GRBs (1 - 6 kpc). We identify two additional MgII absorbers, one of them with a rest frame equivalent width larger than 1A. The distance between the GRB and the absorber measured in this paper confirms that the power of the GRB radiation can influence the conditions of the interstellar medium up to a distance of at least several hundred pc. For the intervening absorbers, we confirm the trend that on average one strong intervening system is found per afterglow, as has been noted in studies exhibiting an excess of strong MgII absorbers along GRB sightlines compared to quasars.Comment: 8 Pages, 7 ps figures, A&A in pres

The high-redshift gamma-ray burst GRB140515A

High-redshift gamma-ray bursts have several advantages for the study of the distant universe, providing unique information about the structure and properties of the galaxies in which they exploded. Spectroscopic identification with large ground-based telescopes has improved our knowledge of the class of such distant events. We present the multi-wavelength analysis of the high-$z$ Swift gamma-ray burst GRB140515A ($z = 6.327$). The best estimate of the neutral hydrogen fraction of the intergalactic medium (IGM) towards the burst is $x_{HI} \leq 0.002$. The spectral absorption lines detected for this event are the weakest lines ever observed in gamma-ray burst afterglows, suggesting that GRB140515A exploded in a very low density environment. Its circum-burst medium is characterised by an average extinction (A$_{\rm V} \sim 0.1$) that seems to be typical of $z \ge 6$ events. The observed multi-band light curves are explained either with a very flat injected spectrum ($p = 1.7$) or with a multi-component emission ($p = 2.1$). In the second case a long-lasting central engine activity is needed in order to explain the late time X-ray emission. The possible origin of GRB140515A from a Pop III (or from a Pop II stars with local environment enriched by Pop III) massive star is unlikely.Comment: 10 pages, 8 figures, 5 tables, submitted to Astronomy & Astrophysic

Factors associated with SARS-CoV-2 infection risk among healthcare workers of an italian university hospital

We report the results of a study on the cumulative incidence of SARS-CoV-2 infections in about 6000 workers of the University Hospital of Modena, Northern Italy, in the period March 2020–January 2021, and the relations with some individual and occupational factors. Overall, in healthcare workers (HCW) the cumulative incidence of COVID-19 during the period was 13.8%. Results confirm the role of overweight and obesity as significant risk factors for SARS-CoV-2 infection. Chronic respiratory diseases, including asthma, also proved to be significantly associated with the infection rate. Considering occupational factors, the COVID-19 risk was about threefold (OR: 2.7; 95% CI 1.7–4.5) greater in nurses and nurse aides than in non-HCW, and about double (OR: 1.9; 95% CI 1.2–3.2) in physicians. Interestingly, an association was also observed between infection risk and nightshifts at work (OR: 1.8; 95% CI 1.4–2.3), significantly related to the total number of shifts in the whole eleven-month period. Even if the vaccination campaign has now greatly modified the scenario of SARS-CoV-2 infections among HCW, the results of this study can be useful for further development of health and policy strategies to mitigate the occupational risk related to the new variants of coronavirus, and therefore the evolution of the pandemic

The warm, the excited, and the molecular gas: GRB 121024A shining through its star-forming galaxy

We present the first reported case of the simultaneous metallicity determination of a gamma-ray burst (GRB) host galaxy, from both afterglow absorption lines as well as strong emission-line diagnostics. Using spectroscopic and imaging observations of the afterglow and host of the long-duration Swift GRB121024A at z = 2.30, we give one of the most complete views of a GRB host/environment to date. We observe a strong damped Ly-alpha absorber (DLA) with a hydrogen column density of log N(HI) = 21.88 +/- 0.10, H2 absorption in the Lyman-Werner bands (molecular fraction of log(f)~ -1.4; fourth solid detection of molecular hydrogen in a GRB-DLA), the nebular emission lines H-alpha, H-beta, [O II], [O III] and [N II], as well as metal absorption lines. We find a GRB host galaxy that is highly star-forming (SFR ~ 40 solar masses/yr ), with a dust-corrected metallicity along the line of sight of [Zn/H]corr = -0.6 +/- 0.2 ([O/H] ~ -0.3 from emission lines), and a depletion factor [Zn/Fe] = 0.85 +/- 0.04. The molecular gas is separated by 400 km/s (and 1-3 kpc) from the gas that is photoexcited by the GRB. This implies a fairly massive host, in agreement with the derived stellar mass of log(M/M_solar ) = 9.9+/- 0.2. We dissect the host galaxy by characterising its molecular component, the excited gas, and the line-emitting star-forming regions. The extinction curve for the line of sight is found to be unusually flat (Rv ~15). We discuss the possibility of an anomalous grain size distributions. We furthermore discuss the different metallicity determinations from both absorption and emission lines, which gives consistent results for the line of sight to GRB 121024A.Comment: 20 pages, 11 figures, accepted by MNRA

A case report of treatment of a streptococcal brain abscess with ceftobiprole supported by the measurement of drug levels in the cerebrospinal fluid

In this paper, we describe the case of a patient admitted to our hospital because of a brain abscess due to Streptococcus intermedius. The management of brain abscess is challenging given the limited potential drug options with effective penetration into both the central nervous system and the abscess capsule to achieve adequate therapeutic concentrations. Due to the high anti-streptococcal activity of ceftobiprole and the availability of ceftobiprole therapeutic drug monitoring in our hospital, we decided to treat the patient with ceftobiprole. To maximize the antimicrobial effect of ceftobiprole, we chose a prolonged intravenous infusion, and we monitored its concentrations in both plasma and cerebrospinal fluid

A quiescent galaxy at the position of the long GRB 050219A

Long-duration gamma-ray bursts (LGRBs) are produced by the collapse of very massive stars. Due to the short lifetime of their progenitors, LGRBs pinpoint star-forming galaxies. We present here a multi-band search for the host galaxy of the long dark GRB 050219A within the enhanced Swift/XRT error circle. We used spectroscopic observations acquired with VLT/X-shooter to determine the redshift and star-formation rate of the putative host galaxy. We compared the results with the optical/IR spectral energy distribution obtained with different facilities. Surprisingly, the host galaxy is a old and quiescent early-type galaxy at z = 0.211 characterised by an unprecedentedly low specific star-formation rate. It is the first LGRB host to be also an early-type post-starburst galaxy. This is further evidence that GRBs can explode in all kind of galaxies, with the only requirement being an episode of star-formation.Comment: 11 pages, 10 figures, 3 tables; accepted for publication in Astronomy & Astrophysic

Ampicillin and Ceftobiprole Combination for the Treatment of Enterococcus faecalis Invasive Infections: “The Times They Are A-Changin”

Background: Enterococcus faecalis is responsible for a large variety of severe infections. This study is a case series reporting our experience in the treatment of E. faecalis invasive infections with ampicillin in combination with ceftobiprole (ABPR). Methods: We retrospectively analyzed all the medical records of patients admitted to the University Hospital of Udine from January to December 2020 with a diagnosis of infective endocarditis or primary or non-primary complicated or uncomplicated bacteremia caused by E. faecalis. Results: Twenty-one patients were included in the final analysis. The clinical success rate was very high, accounting for 81% of patients, and microbiological cure was obtained in 86% of patients. One relapse was recorded in one patient who did not adhere to the partial oral treatment prescribed. Therapeutic drug monitoring (TDM) was always performed for ampicillin and ceftobiprole, and serum concentrations of both drugs were compared to the MICs of the different enterococcal isolates. Conclusions: ABPR is a well-tolerated antimicrobial regimen with anti-E. faecalis activity. TDM can help clinicians optimize medical treatments to achieve the best possible efficacy with fewer side effects. ABPR might be a reasonable option for the treatment of severe invasive infections caused by E. faecalis due to the high level of enterococcal penicillin-binding protein (PBP) saturation