SpxA1 and SpxA2 act coordinately to fine-tune stress responses and virulence in Streptococcus pyogenes

SpxA is a unique transcriptional regulator highly conserved among members of the phylum Firmicutes that binds RNA polymerase and can act as an antiactivator. Why some Firmicutes members have two highly similar SpxA paralogs is not understood. Here, we show that the SpxA paralogs of the pathogen Streptococcus pyogenes, SpxA1 and SpxA2, act coordinately to regulate virulence by fine-tuning toxin expression and stress resistance. Construction and analysis of mutants revealed that SpxA1− mutants were defective for growth under aerobic conditions, while SpxA2− mutants had severely attenuated responses to multiple stresses, including thermal and oxidative stresses. SpxA1− mutants had enhanced resistance to the cationic antimicrobial molecule polymyxin B, while SpxA2− mutants were more sensitive. In a murine model of soft tissue infection, a SpxA1− mutant was highly attenuated. In contrast, the highly stress-sensitive SpxA2− mutant was hypervirulent, exhibiting more extensive tissue damage and a greater bacterial burden than the wild-type strain. SpxA1− attenuation was associated with reduced expression of several toxins, including the SpeB cysteine protease. In contrast, SpxA2− hypervirulence correlated with toxin overexpression and could be suppressed to wild-type levels by deletion of speB. These data show that SpxA1 and SpxA2 have opposing roles in virulence and stress resistance, suggesting that they act coordinately to fine-tune toxin expression in response to stress. SpxA2− hypervirulence also shows that stress resistance is not always essential for S. pyogenes pathogenesis in soft tissue

Heavily obscured AGN in the local Universe

We present here a new powerful diagnostic plot to select heavily obscured AGN in the local universe by combining infrared (Spitzer, IRAS) and X-ray (XMM) information. On the basis of this plot, we selected a sample of X-ray obscured sources in the 2XMM catalogue and found seven newly discovered Compton-thick AGN candidates.Comment: 2 pages, 2 figures, To appear in refereed Proceedings of "X-ray Astronomy 2009: Present Status, Multi-Wavelength Approach and Future Perspectives", Bologna, Italy, September 7-11, 2009, AIP, eds. A. Comastri, M. Cappi, and L. Angelin

The occurrence of the extinct shark genus Sphenodus in the Jurassic of Sicily

During the systematic revision of some historical collections containing Mesozoic ammonites, housed at the "G.G. Gemmellaro" Geological Museum of the Palermo University, a fossil shark’s tooth has been discovered. This specimen, indicated as Lamna in the original catalogue, can be attributed to the genus Sphenodus, an extinct cosmopolitan shark ranging from Lower Jurassic rocks to the Paleocene. The specimen is part of the Mariano Gemmellaro Collection which mainly consists of Middle-Upper Jurassic ammonites coming from Tardàra Mountain, between Menfi and Sambuca di Sicilia (Agrigento Province, Southwestern Sicily). Some of the ammonite specimens were listed, but not illustrated, by M. Gemmellaro in a note of 1919. The succession described in this area consists (from bottom to the top) of Lower Jurassic shallow-water carbonates followed by condensed ammonitic limestones of “Rosso ammonitico-type” (Middle-Late Jurassic in age), Calpionellid limestones (Upper Jurassic-Lower Cretaceous) and cherty calcilutites of Scaglia-type (Upper Cretaceous-Eocene). Since the exact stratigraphic level from which the shark tooth comes is not recorded, a thin section was made of the rock matrix surrounding the tooth. The sedimentological and paleontological analysis of the thin section has highlighted the presence of a microfacies characteristic of the Upper Jurassic condensed deposits of Rosso ammonitico-type, data that fits very well with the geology of the Tardàra area. The study of the Tardàra shark’s tooth has provided both the stimulus and opportunity to undertake a taxonomic review of the Jurassic specimens of Sphenodus collected from a range of Sicilian localities (Gemmellaro G.G., 1871; Seguenza G., 1887; Di Stefano & Cortese, 1891; Seguenza L., 1900; De Gregorio A., 1922) that, to date, have not been re-examined in the light of more recent scholarship. In particular, the specimens described and illustrated by G.G. Gemmellaro (1871), and stored in his eponymous museum, have been revised with the aim of providing a contribution to our knowledge of the genus Sphenodus in the Sicilian Mesozoic successions

Quantitative Effects of Iron Chelators on Hydroxyl Radical Production by the Superoxide-Driven Fenton Raction

Iron bound to certain chelators is known to promote the conversion of superoxide radicals (O2) to hydroxyl radicals (HO\u27) by the superoxide-driven Fenton reaction. The production of HO\u27 by various iron chelates was studied using the reaction of dimethyl sulfoxide and HO\u27 to produce methane sulphinic acid. Methane sulphinic acid was quantified by use of a simple colorimetric assay and used to determine the amounts of HO\u27 produced. Superoxide was generated from 200 M hypoxanthine and 0.05 U/ml xanthine oxidase in the presence of 0-100 M iron and 100 M of each chelator. The results of this preliminary investigation illustrate that, at physiological pH, the superoxide-driven Fenton reaction is significantly promoted by iron chelated to EDTA, nitrilotriacetate, and citrate, but is not promoted by the other anions studied

The Palermo Swift-BAT hard X-ray catalogue III. Results after 54 months of sky survey

We present the Second Palermo Swift-BAT hard X-ray catalogue obtained by analysing data acquired in the first 54 months of the Swift mission. Using our software dedicated to the analysis of data from coded mask telescopes, we analysed the BAT survey data in three energy bands (15-30 keV, 15-70 keV, 15-150 keV), obtaining a list of 1256 detections above a significance threshold of 4.8 standard deviations. The identification of the source counterparts is pursued using two strategies: the analysis of field observations of soft X-ray instruments and cross-correlation of our catalogue with source databases.The survey covers 50% of the sky to a 15--150 keV flux limit of 1.0 x 10^-11 erg s^-1 cm^-2 and 9.2 x 10^-12 erg s^-1 cm^-2 for |b| 10 degrees, respectively. The Second Palermo Swift-BAT hard X-ray catalogue includes 1079 (86%) hard X-ray sources with an associated counterpart (26 with a double association and 2 with a triple association) and 177 BAT excesses (14%) that still lack a counterpart. The distribution of the BAT sources among the different object classes consists of 19% Galactic sources, 57% extragalactic sources, and 10% sources with a counterpart at softer energies whose nature has not yet been determined. About half of the BAT associated sources lack a counterpart in the ROSAT catalogues. This suggests that either moderate or strong absorption may be preventing their detection in the ROSAT energy band. The comparison of our BAT catalogue with the Fermi Large Area Telescope First Source Catalogue identifies 59 BAT/Fermi correspondences: 48 blazars, 3 Seyfert galaxies, 1 interacting galaxy, 3 high mass X-ray binaries, and 4 pulsars/supernova remnants. This small number of correspondences indicates that different populations make the sky shine in these two different energy bands

A semi-automatic system for labelling seafood products and obtaining fishery management data: A case study of the bottom trawl fishery in the central Mediterranean Sea

This study is on the implementation of a semi-automatic labelling system (LS) of the Mediterranean Sea seafood harvest to address the increased need for seafood authentication and inherent difficulties of commonly used indirect techniques for estimating fisheries yield and fishing effort. Sensitive datarequired for anti-counterfeit policies, such as date and catch area, can be acquired and recorded on the label by user-friendly automated software that excludes any possible manipulation by the crew. Based on results obtained from the installation of the LS on bottom commercial trawlers, the system certified the origin of the seafood products and simultaneously provided, indirectly, geospatial fisheries yield and fishing effort data of the main exploited species

Introduction: what is diplomatic security?

History and International Relation