Magnetic-Field Induced Quantum Critical Point in YbRh2_2Si2_2

We report low-temperature calorimetric, magnetic and resistivity measurements on the antiferromagnetic (AF) heavy-fermion metal YbRh$_2$Si$_2$ (${T_N =}$ 70 mK) as a function of magnetic field $B$. While for fields exceeding the critical value ${B_{c0}}$ at which ${T_N\to0}$ the low temperature resistivity shows an ${AT^2}$ dependence, a ${1/(B-B_{c0})}$ divergence of ${A(B)}$ upon reducing $B$ to ${B_{c0}}$ suggests singular scattering at the whole Fermi surface and a divergence of the heavy quasiparticle mass. The observations are interpreted in terms of a new type of quantum critical point separating a weakly AF ordered from a weakly polarized heavy Landau-Fermi liquid state.Comment: accepted for publication in Phys. Rev. Let

A sampling-based strategy for distributing taxis in a road network for occupancy maximization (GIS Cup)

We present a weighted sampling strategy for distributing a system of taxi agents on a road network. We consider a setting, in which each agent operates independently, following a prescribed strategy based on historical data. Furthermore, customer requests appear dynamically and are assigned to the closest unoccupied taxi agent.\u3cbr/\u3e\u3cbr/\u3eWe demonstrate that in this setting a simple sampling strategy based on the spatial distribution of historical data performs well in minimizing the average time that agents are unoccupied. The strategy is evaluated on taxi trip data in Manhattan and compared to various, more complex strategies

Dissymmetrical tunnelling in heavy fermion metals

A tunnelling conductivity between a heavy fermion metal and a simple metallic point is considered. We show that at low temperatures this conductivity can be noticeably dissymmetrical with respect to the change of voltage bias. The dissymmetry can be observed in experiments on the heavy fermion metals whose electronic system has undergone the fermion condensation quantum phase transition.Comment: 7 pages, Revte

Specific heat of heavy fermion CePd2Si2 in high magnetic fields

We report specific heat measurements on the heavy fermion compound CePd2Si2 in magnetic fields up to 16 T and in the temperature range 1.4-16 K. A sharp peak in the specific heat signals the antiferromagnetic transition at T_N ~ 9.3 K in zero field. The transition is found to shift to lower temperatures when a magnetic field is applied along the crystallographic a-axis, while a field applied parallel to the tetragonal c-axis does not affect the transition. The magnetic contribution to the specific heat below T_N is well described by a sum of a linear electronic term and an antiferromagnetic spin wave contribution. Just below T_N, an additional positive curvature, especially at high fields, arises most probably due to thermal fluctuations. The field dependence of the coefficient of the low temperature linear term, gamma_0, extracted from the fits shows a maximum at about 6 T, at the point where an anomaly was detected in susceptibility measurements. The relative field dependence of both T_N and the magnetic entropy at T_N scales as [1-(B/B_0)^2] for B // a, suggesting the disappearance of antiferromagnetism at B_0 ~ 42 T. The expected suppression of the antiferromagnetic transition temperature to zero makes the existence of a magnetic quantum critical point possible.Comment: to be published in Journal of Physics: Condensed Matte

Manipulating adenovirus hexon hypervariable loops dictates immune neutralisation and coagulation factor X-dependent cell interaction in vitro and in vivo

Adenoviruses are common pathogens, mostly targeting ocular, gastrointestinal and respiratory cells, but in some cases infection disseminates, presenting in severe clinical outcomes. Upon dissemination and contact with blood, coagulation factor X (FX) interacts directly with the adenovirus type 5 (Ad5) hexon. FX can act as a bridge to bind heparan sulphate proteoglycans, leading to substantial Ad5 hepatocyte uptake. FX “coating” also protects the virus from host IgM and complement-mediated neutralisation. However, the contribution of FX in determining Ad liver transduction whilst simultaneously shielding the virus from immune attack remains unclear. In this study, we demonstrate that the FX protection mechanism is not conserved amongst Ad types, and identify the hexon hypervariable regions (HVR) of Ad5 as the capsid proteins targeted by this host defense pathway. Using genetic and pharmacological approaches, we manipulate Ad5 HVR interactions to interrogate the interplay between viral cell transduction and immune neutralisation. We show that FX and inhibitory serum components can co-compete and virus neutralisation is influenced by both the location and extent of modifications to the Ad5 HVRs. We engineered Ad5-derived HVRs into the rare, native non FX-binding Ad26 to create Ad26.HVR5C. This enabled the virus to interact with FX at high affinity, as quantified by surface plasmon resonance, FX-mediated cell binding and transduction assays. Concomitantly, Ad26.HVR5C was also sensitised to immune attack in the absence of FX, a direct consequence of the engineered HVRs from Ad5. In both immune competent and deficient animals, Ad26.HVR5C hepatic gene transfer was mediated by FX following intravenous delivery. This study gives mechanistic insight into the pivotal role of the Ad5 HVRs in conferring sensitivity to virus neutralisation by IgM and classical complement-mediated attack. Furthermore, through this gain-of-function approach we demonstrate the dual functionality of FX in protecting Ad26.HVR5C against innate immune factors whilst determining liver targeting

Gametophytic development of Brassica napus pollen in vitroenables examination of cytoskeleton and nuclear movements

Isolated microspores and pollen suspension of Brassica napus “Topas” cultured in NLN-13 medium at 18°C follow gametophytic pathway and develop into pollen grains closely resembling pollen formed in planta. This culture system complemented with whole-mount immunocytochemical technology and novel confocal laser scanning optical technique enables detailed studies of male gametophyte including asymmetric division, cytoskeleton, and nuclear movements. Microtubular cytoskeleton configurationally changed in successive stages of pollen development. The most prominent role of microtubules (MTs) was observed just before and during nuclear migration at the early and mid-bi-cellular stage. At the early bi-cellular stage, parallel arrangement of cortical and endoplasmic MTs to the long axis of the generative cell (GC) as well as MTs within GC under the plasmalemma bordering vegetative cell (VC) were responsible for GC lens shape. At the beginning of the GC migration, endoplasmic microtubules (EMTs) of the VC radiated from the nuclear envelope. Most cortical and EMTs of the VC were found near the sporoderm. At the same time, pattern of MTs observed in GC was considerably different. Multiple EMTs of the GC, previously parallel aligned, reorganized, and start to surround GC, forming a basket-like structure. These results suggest that EMTs of GC provoke changes in GC shape, its detachment from the sporoderm, and play an important role in GC migration to the vegetative nucleus (VN). During the process of migration of the GC to the VC, multiple and thick bundles of MTs, radiating from the cytoplasm near GC plasma membrane, arranged perpendicular to the narrow end of the GC and organized into a “comet-tail” form. These GC “tail” MTs became shortened and the generative nucleus (GN) took a ball shape. The dynamic changes of MTs accompanied polarized distribution pattern of mitochondria and endoplasmic reticulum. In order to confirm the role of MTs in pollen development, a “whole-mount” immunodetection technique and confocal laser-scanning microscopy was essential

Roadmap for implementation of genomics in healthcare: towards equity in access to genomics

As atividades do WP5 do projeto B1MG resultaram num documento intitulado "A Roadmap for genomics in healthcare", que se constitui como um guia e suporte para os sistemas de saúde que pretendam implementar a genómica nos cuidados de saúde, facilitando a utilização do Maturity Level Model e providenciando acesso a guidelines e documentos desenvolvidos no âmbito de outras iniciativas de medicina personalisada. Este poster apresenta o contexto, as atividades e os pontos essenciais que constituem o roadmap.Funding from the European Union’s Horizon 2020 Research and Innovation programme under grant agreement No 951724N/