Time and dose-dependent effects of phenobarbital on the rat liver miRNAome.

In a previous study we had shown that treatment of male Fischer rats with exogenous chemicals for three months resulted in prominent, mode-of-action dependent effects on liver microRNA (miRNA) (Koufaris et al., 2012). Here we investigated how the effects of chemicals on liver miRNA in male Fischer rats relate to the length and dose of exposure to phenobarbital (PB), a drug with multiple established hepatic effects. Importantly, although acute PB treatment (1-7 days) had significant effects on liver mRNA and the expected effects on the liver phenotype (transient hyperplasia, hepatomegaly, cytochrome P450 induction), limited effects on liver miRNA were observed. However, at 14 days of PB treatment clear dose-dependent effects on miRNA were observed. The main effect of PB treatment from days 1 to 90 on liver miRNA was found to be the persistent, progressive, and highly correlated induction of the miR-200a/200b/429 and miR-96/182 clusters, occurring after the termination of the xenobiotic-induced transient hyperplasia. Moreover, in agreement with their reported functions in the literature we found associations between perturbations of miR-29b and miR-200a/200b by PB with global DNA methylation and zeb1/zeb2 proteins respectively. Our data suggest that miRNA are unlikely to play an important role in the acute responses of the adult rodent liver to PB treatment. However, the miRNA responses to longer PB exposures suggest a potential role for maintaining liver homeostasis in response to sub-chronic and chronic xenobiotic-induced perturbations. Similar studies for more chemicals are needed to clarify whether the temporal and dose pattern of miRNA-toxicant interaction identified here for PB are widely applicable to other xenobiotics. © 2013 Elsevier Ireland Ltd

Are you serious? From fist bumping to hand hygiene: considering culture, context and complexity in infection prevention intervention research

Infection prevention is an under-resourced research and development topic, with limited evidence for practice in the most basic of measures. A survey of IPS R&D members indicated that what might appear to be simple interactions and interventions in healthcare, such as hand shaking and hand hygiene, should be considered complex interventions taking account of behaviour at the individual and social level as well as contextual factors. Future studies need to be designed utilising comprehensive approaches, for example, the Medical Research Council complex interventions framework, tailored to the country and more local cultural context, if we are to be serious about evidence for infection prevention and control practice

Gender inequality and sex differences in physical fighting, physical activity, and injury among adolescents across 36 countries

Purpose: Sex differences in adolescent health are widely documented, but social explanations for these sex differences are scarce. This study examines whether societal gender inequality (i.e., men’s and women’s unequal share in political participation, decision-making power, economic participation and command over resources) relates to sex differences in adolescent physical fighting, physical activity, and injuries. Methods: National-level data on gender inequality (i.e. the United Nations Development Program’s Gender Inequality Index) were linked to health data from 71,255 15-year olds from 36 countries in the 2009/10 Health Behavior in School-aged Children (HBSC) study. Using multilevel logistic regression analyses, we tested the association between gender inequality and sex differences in health while controlling for country wealth (GDP per capita). Results: In all countries, boys reported more physical fighting, physical activity, and injuries than girls, but the magnitude of these sex differences varied greatly between countries. Societal gender inequality positively related to sex differences in all three outcomes. In more gender unequal countries, boys reported higher levels of fighting and physical activity, compared to boys in more gender equal countries. In girls, scores were consistently low for these outcomes, however injury was more common in countries with less gender inequality. Conclusions: Societal gender inequality appears to relate to sex differences in some adolescent health behaviors and may contribute to the establishment of sex differences in morbidity and mortality. To reduce inequalities in the health of future generations, public health policy should target social and cultural factors that shape perceived gender norms in young people

An archival case study : revisiting the life and political economy of Lauchlin Currie

This paper forms part of a wider project to show the significance of archival material on distinguished economists, in this case Lauchlin Currie (1902-93), who studied and taught at Harvard before entering government service at the US Treasury and Federal Reserve Board as the intellectual leader of Roosevelt's New Deal, 1934-39, as FDR's White House economic adviser in peace and war, 1939-45, and as a post-war development economist. It discusses the uses made of the written and oral material available when the author was writing his intellectual biography of Currie (Duke University Press 1990) while Currie was still alive, and the significance of the material that has come to light after Currie's death

Melioidosis in Papua New Guinea and Oceania

Melioidosis has only been sporadically reported throughout Melanesia and the Pacific region since the first report from Guam in 1946; therefore, its contribution to the disease burden in this region is largely unknown. However, the outcome of a small number of active surveillance programs, serological surveys, and presumptive imported cases identified elsewhere provide an insight into its epidemiology and potential significance throughout the region. Both clinical cases and environmental reservoirs have been described from the rural district of Balimo in the Western Province of Papua New Guinea and from the Northern Province of New Caledonia. In both these locations the incidence of disease is similar to that described in tropical Australia and Burkholderia pseudomallei isolates are also phylogenetically linked to Australian isolates. Serological evidence and presumptive imported cases identified elsewhere suggest that melioidosis exists in other countries throughout the Pacific. However, the lack of laboratory facilities and clinical awareness, and the burden of other infections of public health importance such as tuberculosis, contribute to the under-recognition of melioidosis in this region

Confirmation of the planet around HD 95086 by direct imaging

VLT/NaCo angular differential imaging at L' (3.8 microns) revealed a probable giant planet comoving with the young and early-type HD 95086 also known to harbor an extended debris disk. The discovery was based on the proper motion analysis of two datasets spanning 15 months. However, the second dataset suffered from bad atmospheric conditions, which limited the significance of the redetection at the 3 sigma level. In this Letter, we report new VLT/NaCo observations of HD 95086 obtained on 2013 June 26-27 at L' to recover the planet candidate. We unambiguously redetect the companion HD 95086 b with multiple independent pipelines at a signal-to-noise ratio greater than or equal to 5. Combined with previously reported measurements, our astrometry decisively shows that the planet is comoving with HD 95086 and inconsistent with a background object. With a revised mass of 5 pm 2 Jupiter masses, estimated from its L' photometry and "hot-start" models at 17 pm 4 Myr, HD 95086 b becomes a new benchmark for further physical and orbital characterization of young giant planets.Comment: accepted for publication to AP

Remarks on separating words

The separating words problem asks for the size of the smallest DFA needed to distinguish between two words of length <= n (by accepting one and rejecting the other). In this paper we survey what is known and unknown about the problem, consider some variations, and prove several new results