The influence of delirium on mortality and length of ICU stay and analysis of risk factors for delirium after liver transplantation

ObjectiveTo analyze the incidence, timing, risk factors and prognosis of delirium after liver transplantation (LT).MethodsThe clinical data of 321 patients undergoing LT in the Third Xiangya Hospital of Central South University from January 2018 to December 2022 were collected to investigate the incidence, onset, and risk factors for post-LT delirium and the impact of delirium on LT recipients’ prognosis by statistical analysis.ResultsThe incidence of post-LT delirium was 19.3% (62/321), and the median interval between LT and onset of delirium was 20.1 h. Univariate analysis showed that pre-LT variables (Model for End Stage Liver Disease (MELD) score, hospital stay, hepatic encephalopathy, infection, white blood cell (WBC) count, lymphocyte count, abnormal potassium, lactulose use), intraoperative variables (red blood cell transfusion, remimazolam use, dexmedetomidine use) and post-LT variables (hypernatraemia, acute rejection, reoperation, basiliximab use, tacrolimus concentration) were associated with post-LT delirium. Multivariate logistic regression analysis revealed that MELD score at LT ≥22 [OR = 3.400, 95% CI:1.468–7.876, p = 0.004], pre-LT hepatic encephalopathy [OR = 3.224, 95% CI:1.664–6.244, p = 0.001], infection within 2 months prior to LT [OR = 2.238, 95% CI:1.151–4.351, p = 0.018], acute rejection [OR = 2.974, 95% CI:1.322–6.690, p = 0.008], and reoperation [OR = 11.919, 95% CI:2.938–48.350, p = 0.001] were independent risk factors for post-LT delirium. Post-LT delirium was reduced in LT recipients exposing to intraoperative remimazolam [OR = 0.287, 95% CI: 0.113–0.733, p = 0.009] or ≥ 25 μg of intraoperative dexmedetomidine [OR = 0.441, 95% CI 0.225–0.867, p = 0.018]. As for clinical outcomes, patients with delirium had a higher percentage of staying at the (ICU) ≥7 d after LT than those without delirium [OR = 2.559, 95% CI 1.418–4.617, p = 0.002].ConclusionThe incidence of delirium was high and the onset of delirium was early after LT. Risk factors for post-LT delirium included high MELD score at LT, pre-LT hepatic encephalopathy and infections, acute rejection and reoperation. Intraoperative use of remimazolam or dexmedetomidine reduced post-LT delirium. Delirium had a negative impact on the length of ICU stay

Tree-ring based precipitation variability since AD 1828 in northwestern Liaoning, China

A 183-year record of total precipitation from September to current July was reconstructed using tree rings from Chinese pine (Pinus tabulaeformis) to explore regional moisture variations in northwestern Liaoning province. The reconstruction accounts for 35.4% of the total variance of the instrumental precipitation from 1957 to 2010. The reconstruction shows eleven persistent dry periods (e.g. 1856–1866, 1886–1891, 1898–1905), and eleven persistent wet periods (e.g. 1835–1855, 1867–1878, 1892–1897). Spectral analysis of the reconstruction shows several significant spectral peaks in the ca. 2–4-year periodicity band, suggesting inter-annual variability of El Niño-Southern Oscillation (ENSO). The precipitation reconstruction shows correlations related to the East Asian Monsoon and possible teleconnection of regional moisture variations to Pacific Ocean signals, such as El Niño and La Niña

Serum N-glycome biomarker for monitoring development of DENA-induced hepatocellular carcinoma in rat

<p>Abstract</p> <p>Background</p> <p>There is a demand for serum markers for the routine assessment of the progression of liver cancer. We previously found that serum N-linked sugar chains are altered in hepatocellular carcinoma (HCC). Here, we studied glycomic alterations during development of HCC in a rat model.</p> <p>Results</p> <p>Rat HCC was induced by the hepatocarcinogen, diethylnitrosamine (DENA). N-glycans were profiled using the DSA-FACE technique developed in our laboratory.</p> <p>In comparison with control rats, DENA rats showed a gradual but significant increase in two glycans (R5a and R5b) in serum total N-glycans during progression of liver cirrhosis and cancer, and a decrease in a biantennary glycan (P5). The log of the ratio of R5a to P1 (NGA2F) and R5b to P1 [log(R5a/P1) and log(R5b/P1)] were significantly (p < 0.0001) elevated in HCC rats, but not in rats with cirrhosis or fibrosis or in control rats. We thus propose a GlycoTest model using the above-mentioned serum glycan markers to monitor the progression of cirrhosis and HCC in the DENA-treated rat model. When DENA-treated rats were subsequently treated with farnesylthiosalicyclic acid, an anticancer drug, progression to HCC was prevented and GlycoTest markers (P5, R5a and R5b) reverted towards non-DENA levels, and the HCC-specific markers, log(R5a/P1) and log(R5b/P1), normalized completely. <b>Conclusions</b>: We found an increase in core-α-1,6-fucosylated glycoproteins in serum and liver of rats with HCC, which demonstrates that fucosylation is altered during progression of HCC. Our GlycoTest model can be used to monitor progression of HCC and to follow up treatment of liver tumors in the DENA rat. This GlycoTest model is particularly important because a rapid non-invasive diagnostic procedure for tumour progression in this rat model would greatly facilitate the search for anticancer drugs.</p

(1)H, (13)C, (15)N backbone and side-chain resonance assignments of the human Raf-1 kinase inhibitor protein

Raf-1 kinase inhibitor protein (RKIP) plays a pivotal role in modulating multiple signaling networks. Here we report backbone and side chain resonance assignments of uniformly (15)N, (13)C labeled human RKIP.Natural Science Foundation of China[30900233, 30730026]; Program of Shanghai Subject Chief Scientist[09XD1405100

Basel Problem – Preliminaries

SummaryIn the article we formalize in the Mizar system [4] preliminary facts needed to prove the Basel problem [7, 1]. Facts that are independent from the notion of structure are included here.Korniłowicz Artur - Institute of Informatics, University of Białystok, PolandPąk Karol - Institute of Informatics, University of Białystok, PolandM. Aigner and G. M. Ziegler. Proofs from THE BOOK. Springer-Verlag, Berlin Heidelberg New York, 2004.Grzegorz Bancerek. The fundamental properties of natural numbers. Formalized Mathematics, 1(1):41–46, 1990.Grzegorz Bancerek and Krzysztof Hryniewiecki. Segments of natural numbers and finite sequences. Formalized Mathematics, 1(1):107–114, 1990.Grzegorz Bancerek, Czesław Byliński, Adam Grabowski, Artur Korniłowicz, Roman Matuszewski, Adam Naumowicz, Karol Pąk, and Josef Urban. Mizar: State-of-the-art and beyond. In Manfred Kerber, Jacques Carette, Cezary Kaliszyk, Florian Rabe, and Volker Sorge, editors, Intelligent Computer Mathematics, volume 9150 of Lecture Notes in Computer Science, pages 261–279. Springer International Publishing, 2015. ISBN 978-3-319-20614-1. doi: 10.1007/978-3-319-20615-817.Czesław Byliński. Finite sequences and tuples of elements of a non-empty sets. Formalized Mathematics, 1(3):529–536, 1990.Czesław Byliński. Functions and their basic properties. Formalized Mathematics, 1(1): 55–65, 1990.Augustin Louis Cauchy. Cours d’analyse de l’Ecole royale polytechnique. de l’Imprimerie royale, 1821.Wenpai Chang, Yatsuka Nakamura, and Piotr Rudnicki. Inner products and angles of complex numbers. Formalized Mathematics, 11(3):275–280, 2003.Wenpai Chang, Hiroshi Yamazaki, and Yatsuka Nakamura. The inner product and conjugate of finite sequences of complex numbers. Formalized Mathematics, 13(3):367–373, 2005.Noboru Endou. Double series and sums. Formalized Mathematics, 22(1):57–68, 2014. doi: 10.2478/forma-2014-0006.Noboru Endou, Katsumi Wasaki, and Yasunari Shidama. Definition of integrability for partial functions from ℝ to ℝ and integrability for continuous functions. Formalized Mathematics, 9(2):281–284, 2001.Adam Grabowski and Yatsuka Nakamura. Some properties of real maps. Formalized Mathematics, 6(4):455–459, 1997.Artur Korniłowicz and Yasunari Shidama. Inverse trigonometric functions arcsin and arccos. Formalized Mathematics, 13(1):73–79, 2005.Jarosław Kotowicz. Partial functions from a domain to the set of real numbers. Formalized Mathematics, 1(4):703–709, 1990.Xiquan Liang and Bing Xie. Inverse trigonometric functions arctan and arccot. Formalized Mathematics, 16(2):147–158, 2008. doi: 10.2478/v10037-008-0021-3.Robert Milewski. Trigonometric form of complex numbers. Formalized Mathematics, 9 (3):455–460, 2001.Keiichi Miyajima and Takahiro Kato. The sum and product of finite sequences of complex numbers. Formalized Mathematics, 18(2):107–111, 2010. doi: 10.2478/v10037-010-0014-x.Cuiying Peng, Fuguo Ge, and Xiquan Liang. Several integrability formulas of special functions. Formalized Mathematics, 15(4):189–198, 2007. doi: 10.2478/v10037-007-0023-6.Konrad Raczkowski. Integer and rational exponents. Formalized Mathematics, 2(1):125–130, 1991.Konrad Raczkowski and Paweł Sadowski. Real function continuity. Formalized Mathematics, 1(4):787–791, 1990.Piotr Rudnicki and Andrzej Trybulec. Abian’s fixed point theorem. Formalized Mathematics, 6(3):335–338, 1997.Yasunari Shidama, Noboru Endou, and Katsumi Wasaki. Riemann indefinite integral of functions of real variable. Formalized Mathematics, 15(2):59–63, 2007. doi: 10.2478/v10037-007-0007-6.Andrzej Trybulec and Czesław Byliński. Some properties of real numbers. Formalized Mathematics, 1(3):445–449, 1990.Michał J. Trybulec. Integers. Formalized Mathematics, 1(3):501–505, 1990.Edmund Woronowicz. Relations and their basic properties. Formalized Mathematics, 1 (1):73–83, 1990.Yuguang Yang and Yasunari Shidama. Trigonometric functions and existence of circle ratio. Formalized Mathematics, 7(2):255–263, 1998.25214114

Effects of bone marrow mesenchymal stromal cells-derived therapies for experimental traumatic brain injury： A meta-analysis

Background: Bone-marrow-derived mesenchymal stromal (stem) cells [also called MSC(M)] and their extracellular vesicles (EVs) are considered a potentially innovative form of therapy for traumatic brain injury (TBI). Nevertheless, their application to TBI particularly remains preclinical, and the effects of these cells remain unclear and controversial. Therefore, an updated meta-analysis of preclinical studies is necessary to assess the effectiveness of MSC(M) and MSC(M) derived EVs in clinical trials. Methods: The following databases were searched (to December 2022): PubMed, Web of Science, and Embase. In this study, we measured functional outcomes based on the modified neurological severity score (mNSS), cognitive outcomes based on the Morris water maze (MWM), and histopathological outcomes based on lesion volume. A random effects meta-analysis was conducted to evaluate the effect of mNSS, MWM, and lesion volume. Results: A total of 2163 unique records were identified from our search, with Fifty-five full-text articles satisfying inclusion criteria. A mean score of 5.75 was assigned to the studies’ quality scores, ranging from 4 to 7. MSC(M) and MSC(M) derived EVs had an overall positive effect on the mNSS score and MWM with SMDs −2.57 (95 % CI -3.26; −1.88; p < 0.01) and - 2.98 (95 % CI -4.21; −1.70; p < 0.01), respectively. As well, MSC(M) derived EVs were effective in reducing lesion volume by an SMD of - 0.80 (95 % CI -1.20; −0.40; p < 0.01). It was observed that there was significant variation among the studies, but further analyses could not determine the cause of this heterogeneity. Conclusions: MSC(M) and MSC(M) derived EVs are promising treatments for TBI in pre-clinical studies, and translation to the clinical domain appears warranted. Besides, large-scale trials in animals and humans are required to support further research due to the limited sample size of MSC(M) derived EVs

Selective HDAC6 inhibitor TubA offers neuroprotection after intracerebral hemorrhage via inhibiting neuronal apoptosis

A large body of evidence has demonstrated that neuronal apoptosis is involved in the pathological process of secondary brain injury following intracerebral hemorrhage (ICH). Additionally, our previous studies determined that the inhibition of HDAC6 activity by tubacin or specific shRNA can attenuate neuronal apoptosis in an oxygen-glucose deprivation reperfusion model. However, whether the pharmacological inhibition of HDAC6-attenuated neuronal apoptosis in ICH remains unclear. In this study, we used hemin-induced SH-SY5Y cells to simulate a hemorrhage state in vitro and adopted a collagenase-induced ICH rat model in vivo to assess the effect of the HDAC6 inhibition. We found a significant increase in HDAC6 during the early stages of ICH. As expected, the acetylated α-tubulin significantly decreased in correlation with the expression of HDAC6. Medium and high doses (25, 40 mg/kg) of TubA, a selective inhibitor of HDAC6, both reduced neurological impairments, histological impairments, and ipsilateral brain edema in vivo. TubA or HDAC6 siRNA both alleviated neuronal apoptosis in vivo and in vitro. Finally, HDAC6 inhibition increased the level of acetylated α-tubulin and Bcl-2 and lowered the expression of Bax and cleaved caspase-3 post-ICH. In general, these results suggested that the pharmacological inhibition of HDAC6 may act as a novel and promising therapeutic target for ICH therapy by up-regulating acetylated α-tubulin and reducing neuronal apoptosis

Characterization of the complete chloroplast genome sequence of Tinospora sagittata and its phylogenetic implications

Tinospora sagittata is a perennial vine of the family Menispermaceae and distributed in Hunan, Hubei, Guangxi, and Sichuan province of P. R. China. It has been used in Chinese traditional medicine for centuries. The chloroplast (cp) genome of T. sagittata, characterized using Illumina technology, is 163,662 bp in size. There are a total of 130 genes, coding for 85 proteins, 37 tRNAs, and 8 rRNAs. Phylogenetic relationship analysis based on 16 complete cp genome sequences exhibited that T. sagittata was phylogenetically closer to Menispermum dauricum and Stephania japonica

The complete chloroplast genome sequence of Calanthe sieboldii (orchidaceae)

AbstractCalanthe sieboldii Decne. ex Regel is a terrestrial orchid with high ornamental and commercial value. In the present study, the chloroplast genome of C. sieboldii was characterized using Illumina technology. The chloroplast genome is 158,345 bp in length with a total AT content of 63.28%. There are 127 genes, comprising 37 tRNA genes, 82 protein-coding genes, and 8 rRNA genes. Phylogenetic relationship analysis was performed using common protein-coding genes extracted from 13 chloroplast genomes of Orchidaceae. It was revealed that C. sieboldi was sister to C. hancockii and closely clustered with C. aristulifera and C. henryi. These findings provide valuable genomic resources that are helpful for further phylogenetic and evolutionary studies of Calanthe