The Economics of Tobacco and Tobacco Control

This monograph, a joint effort of the U.S. National Cancer Institute and World Health Organization, examines economic issues in tobacco and tobacco control, including the supply and demand of tobacco products. This first chapter frames the issues addressed in the monograph and describes its organization around key topic areas. Each monograph chapter focuses on the global evidence on these issues, particularly the evidence from low- and middle-income countries (LMICs). The closing sections of this chapter present chapter conclusions and major overall conclusions generated by the work presented here. Experts in economics, tobacco control, public policy, public health, and other related fields from every region in the world, including high-income countries and LMICs, were assembled to provide the research and analyses presented within these pages. It is hoped that this monograph will help inform the implementation of global tobacco control efforts in the 21st century.Additional co-authors: Dongbo Fu, C.K. Gajalakshmi, Vendhan Gajalakshmi, Mark Goodchild, Emmanuel Guindon, Prakash Gupta, Reviva Hasson, Luminita S Hayes, Sara Hitchman, Kinh Hoang-Van, Jidong Huang, Andrew Hyland, Nathan Jones, John Keyser, Pierre Kopp, Harry Lando, David Levy, James Lightwood, Christine Logel, Benn McGrady, Yumiko Mochizuki-Kobayashi, Mario Monsour, Nigar Nargis, Richard J. O’Connor, Maizurah Omar, Zeynep Önder, William Onzivu, Anne-Marie Perucic, Armando Peruga, Vinayak M. Prasad, Martin Raw, Cecily S. Ray, Lyn Reed, Bung-on Ritthiphakdee, Hana Ross, Jennifer Ruger, Henry Saffer, Genevieve Sansone, Natalie Sansone, Fatwa Sari Tetra Dewi, Kerstin Schotte, Omar Shafey, Yoon-Jeong Shin, Giorgio Sincovich, John Tauras, Mark Travers, Édouard Tursan d’Espaignet, Marco Vargas, Mandeep K. Virk-Baker, Corné van Walbeek, Charles W. Warren, Marzenna Anna Weresa, Xin Xu, Eduard Zaloshnja, Lei Zhang, Ping Zhan

Chocolate consumption and risk of gestational diabetes mellitus: the Japan Environment and Children’s Study

The association of chocolate consumption with risk of gestational diabetes has not been examined. We aimed to investigate the prospective association between chocolate consumption and risk of gestational diabetes in a large birth cohort in Japan. A total of 97 454 pregnant women with a median gestational age of 12 weeks were recruited from January 2011 to March 2014. Data on demographic information, disease history, socio-economic status, lifestyle and dietary habits were obtained at the study enrolment. Dietary intake during the past 12 months before study enrolment was assessed through a semi-quantitative FFQ. The logistic regression was used to obtain the OR of gestational diabetes in relation to chocolate consumption. Among 84 948 women eligible for the analysis, 1904 cases of gestational diabetes (2·2 %) were identified during the period of pregnancy. After controlling for potential confounding factors including age, smoking status, drinking status, education level, occupation, pre-pregnant BMI, depression, previous history of macrosomia babies, parity, physical activity and dietary factors, women in the highest quartile of chocolate consumption, compared with those in the lowest quartile, had a significantly lower risk of developing gestational diabetes (OR 0·78, 95 % CI 0·67, 0·90; P for trend = 0·002). Stratified analyses suggested that the association was not significantly modified by pre-pregnancy BMI, age, parity, smoking status or drinking status. The present prospective cohort study provided evidence that chocolate consumption was associated with a significant lower risk of gestational diabetes in Japanese women

Finishing the euchromatic sequence of the human genome

The sequence of the human genome encodes the genetic instructions for human physiology, as well as rich information about human evolution. In 2001, the International Human Genome Sequencing Consortium reported a draft sequence of the euchromatic portion of the human genome. Since then, the international collaboration has worked to convert this draft into a genome sequence with high accuracy and nearly complete coverage. Here, we report the result of this finishing process. The current genome sequence (Build 35) contains 2.85 billion nucleotides interrupted by only 341 gaps. It covers ∼99% of the euchromatic genome and is accurate to an error rate of ∼1 event per 100,000 bases. Many of the remaining euchromatic gaps are associated with segmental duplications and will require focused work with new methods. The near-complete sequence, the first for a vertebrate, greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death. Notably, the human enome seems to encode only 20,000-25,000 protein-coding genes. The genome sequence reported here should serve as a firm foundation for biomedical research in the decades ahead

Effect of Spent Mushroom Substrate on Physical and Chemical Properties and Enzymic Activity of Rice

In order to explore the substitution substrate for rice seedling on upland fields, this paper uses spent mushroom substrate to study the physical and chemical properties of substrate, enzymic activity and number of tillers during the cultivation of rice seedling on upland fields. The results show that at the three stages of rice seedling cultivation (two-leaf stage, three-leaf stage, four-leaf stage), the content of organic matter and EC in spent mushroom substrate is higher than in the control soil, pH is within the range suitable for the growth of rice, and other nutrients (total nitrogen, total phosphorus, total potassium, available nitrogen, available phosphorus) are slightly different in different periods; except phosphatase, there are significant differences in urease, catalase and sucrase between spent mushroom substrate and the control soil; the number of tillers under spent mushroom substrate is larger than under the control

Prevalence and Correlates of Dyslipidemia Among Men and Women in Palau: Findings of the Palau STEPS Survey 2011–2013

Background: Epidemiological evidence of dyslipidemia in Pacific Island countries is limited despite the knowledge that non-communicable diseases have a high burden in the region. We aimed to examine the prevalence and correlates of dyslipidemia among residents of Palau. Methods: The Palau STEPwise approach to Surveillance (STEPS), which was conducted from 2011 through 2013, comprised three parts: behavioral risk factors; physical measurements; and biochemical tests, covering areas such as blood lipids. We used STEPS-generated data to perform a cross-sectional study of 2,184 randomly selected Palau residents, comprising Palauans and non-Palauans aged 25–64 years. Results: The age-adjusted mean BMI was 29.3 kg/m2 in men and 29.9 kg/m2 in women; age-adjusted mean triglycerides value was 182 mg/dL in men and 166 mg/dL in women; and age-adjusted mean cholesterol was 178 mg/dL in men and 183 mg/dL in women. The prevalence of overweight/obesity (BMI ≥25 kg/m2) was 75% in men and 76% in women, and those of hypertriglyceridemia (triglycerides ≥150 mg/dL) and hypercholesterolemia (total cholesterol ≥200 mg/dL) were 48% in men and 41% in women and 18% in men and 23% in women, respectively. Mean values of total cholesterol were 177 mg/dL in Palauan men and 182 mg/dL in non-Palauan men. Mean values of triglycerides were 171 mg/dL in Palauan women and 150 mg/dL in non-Palauan women. Women living in rural areas showed a higher mean value of total cholesterol than those in urban areas. Conclusion: We found a high mean BMI and high prevalence of overweight/obesity and hypertriglyceridemia, but low mean total cholesterol and a low prevalence of hypercholesterolemia in Palau. Lipid profiles varied by age, ethnicity, and living area

Protein Engineering of Pasteurella multocida alpha 2,3-Sialyltransferase with Reduced alpha 2,3-Sialidase Activity and Application in Synthesis of 3 '-Sialyllactose

Sialyltransferases are key enzymes for the production of sialosides. The versatility of Pasteurella multocida alpha 2,3-sialyltransferase 1 (PmST1) causes difficulties in the efficient synthesis of alpha 2,3-linked sialylatetd compounds, especial its alpha 2,3-sialidase activity. In the current study, the alpha 2,3-sialidase activity of PmST1 was further reduced by rational design-based protein engineering. Three double mutants PMG1 (M144D/R313Y), PMG2 (M144D/R313H) and PMG3 (M144D/R313N) were designed and constructed using M144D as the template and kinetically investigated. In comparison with M144D, the alpha 2,3-sialyltransferase activity of PMG2 was enhanced by 1.4-fold, while its alpha 2,3-sialidase activity was reduced by 4-fold. Two PMG2-based triple mutants PMG2-1 (M144D/R313H/T265S) and PMG2-2 (M144D/R313H/E271F) were then designed, generated and characterized. Compared with PMG2, triple mutants showed slightly improved alpha 2,3-sialyltransferase activity, but their alpha 2,3-sialidase activities were increased by 2.1-2.9 fold. In summary, PMG2 was used for preparative-scale production of 3 '-SL (3 '-sialyllactose) with a yield of >95%. These new PmST1 mutants could be potentially utilized for efficient synthesis of alpha 2,3-linked sialosides. This work provides a guide to designing and constructing efficient sialyltransferases

Protein Engineering of Pasteurella multocida α2,3-Sialyltransferase with Reduced α2,3-Sialidase Activity and Application in Synthesis of 3′-Sialyllactose

Sialyltransferases are key enzymes for the production of sialosides. The versatility of Pasteurella multocida α2,3-sialyltransferase 1 (PmST1) causes difficulties in the efficient synthesis of α2,3-linked sialylatetd compounds, especial its α2,3-sialidase activity. In the current study, the α2,3-sialidase activity of PmST1 was further reduced by rational design-based protein engineering. Three double mutants PMG1 (M144D/R313Y), PMG2 (M144D/R313H) and PMG3 (M144D/R313N) were designed and constructed using M144D as the template and kinetically investigated. In comparison with M144D, the α2,3-sialyltransferase activity of PMG2 was enhanced by 1.4-fold, while its α2,3-sialidase activity was reduced by 4-fold. Two PMG2-based triple mutants PMG2-1 (M144D/R313H/T265S) and PMG2-2 (M144D/R313H/E271F) were then designed, generated and characterized. Compared with PMG2, triple mutants showed slightly improved α2,3-sialyltransferase activity, but their α2,3-sialidase activities were increased by 2.1–2.9 fold. In summary, PMG2 was used for preparative-scale production of 3′-SL (3′-sialyllactose) with a yield of >95%. These new PmST1 mutants could be potentially utilized for efficient synthesis of α2,3-linked sialosides. This work provides a guide to designing and constructing efficient sialyltransferases

Protein Engineering of Pasteurella multocida alpha 2,3-Sialyltransferase with Reduced alpha 2,3-Sialidase Activity and Application in Synthesis of 3 '-Sialyllactose

Sialyltransferases are key enzymes for the production of sialosides. The versatility of Pasteurella multocida alpha 2,3-sialyltransferase 1 (PmST1) causes difficulties in the efficient synthesis of alpha 2,3-linked sialylatetd compounds, especial its alpha 2,3-sialidase activity. In the current study, the alpha 2,3-sialidase activity of PmST1 was further reduced by rational design-based protein engineering. Three double mutants PMG1 (M144D/R313Y), PMG2 (M144D/R313H) and PMG3 (M144D/R313N) were designed and constructed using M144D as the template and kinetically investigated. In comparison with M144D, the alpha 2,3-sialyltransferase activity of PMG2 was enhanced by 1.4-fold, while its alpha 2,3-sialidase activity was reduced by 4-fold. Two PMG2-based triple mutants PMG2-1 (M144D/R313H/T265S) and PMG2-2 (M144D/R313H/E271F) were then designed, generated and characterized. Compared with PMG2, triple mutants showed slightly improved alpha 2,3-sialyltransferase activity, but their alpha 2,3-sialidase activities were increased by 2.1-2.9 fold. In summary, PMG2 was used for preparative-scale production of 3 '-SL (3 '-sialyllactose) with a yield of >95%. These new PmST1 mutants could be potentially utilized for efficient synthesis of alpha 2,3-linked sialosides. This work provides a guide to designing and constructing efficient sialyltransferases

Associations of Tobacco Smoking with Impaired Endothelial Function: The Circulatory Risk in Communities Study (CIRCS)

Aims: Smoking impairs endothelial function as an acute effect. However, few population-based studies have examined the association between smoking status and endothelial function or the dose–response and duration–response association of smoking with endothelial function. We examined whether smoking habits were associated with impaired endothelial function depending on smoking dose and duration.Methods: We conducted a cross-sectional study of 910 men and women aged 30–79 years from 2013 to 2016. Statistical analyses of the data were conducted between 2016 and 2017. Endothelial function was assessed by brachial artery flow-mediated dilation (FMD) measurement. Low FMD was defined in two ways as the cutoff point based on the lowest quartile of %FMD (＜5.1%) and median of %FMD (＜6.8%), regarding as impaired endothelial function. We investigated the smoking status in terms of cigarettes consumed per day and the duration of smoking.Results: Heavy and chronic smokers were associated with a high prevalence of impaired endothelial function. Those associations did not change substantially after adjustment for other cardiovascular risk factors. Among all participants, the multivariable-adjusted ORs (95% CIs) of low FMD (＜5.1%) with reference to never smokers were 2.23 (1.00–5.14) for current heavy smokers of ≥ 30 cigarettes per day, 1.83 (1.04–3.20) for heavy smokers of ≥ 40 pack-years, and 2.16 (1.15–4.06) for chronic smokers of ≥ 40 years. For low FMD (＜6.8%) those values was 2.17 (1.01–5.05), 1.70 (1.01–2.86), and 1.98 (1.07–3.69), respectively.Conclusions: Similar associations were observed among only men. Heavy or long-term tobacco smoking may induce impaired endothelial function