Predictive Fault Diagnosis for Ship Photovoltaic Modules Systems Applications

[EN] In this paper, an application for the management and supervision by predictive fault diagnosis (PFD) of solar power generation systems is developed through a National Marine Electronics Association (NMEA) 2000 smart sensor network. Here, the NMEA 2000 network sensor devices for measuring and supervising the parameters inherent to solar power generation and renewable energy supply are applied. The importance of renewable power generation systems in ships is discussed, as well as the causes of photovoltaic modules (PVMs) aging due to superimposed causes of degradation, which is a natural and inexorable phenomenon that affects photovoltaic installations in a special way. In ships, PVMs are doubly exposed to inclement weather (solar radiation, cold, rain, dust, humidity, snow, wind, electrical storms, etc.), pollution, and a particularly aggressive environment in terms of corrosion. PFD techniques for the real-world installation and safe navigation of PVMs are discussed. A specific method based on the online analysis of the time-series data of random and seasonal I¿V parameters is proposed for the comparative trend analyses of solar power generation. The objective is to apply PFD using as predictor symptom parameter (PS) the generated power decrease in affected PVMs. This PFD method allows early fault detection and isolation, whose appearance precedes by an adequate margin of maneuver, from the point of view of maintenance tasks applications. This early detection can stop the cumulative degradation phenomenon that causes the development of the most frequent and dangerous failure modes of solar modules, such as hot-spots. It is concluded that these failure modes can be conveniently diagnosed by performing comparative trend analyses of the measured power parameters by NMEA sensors.García Moreno, E.; Quiles Cucarella, E.; Zotovic Stanisic, R.; Gutiérrez, SC. (2022). Predictive Fault Diagnosis for Ship Photovoltaic Modules Systems Applications. Sensors. 22(6):1-21. https://doi.org/10.3390/s2206217512122

Solar Panels String Predictive and Parametric Fault Diagnosis Using Low-Cost Sensors

[EN] This work proposes a method for real-time supervision and predictive fault diagnosis applicable to solar panel strings in real-world installations. It is focused on the detection and parametric isolation of fault symptoms through the analysis of the Voc-Isc curves. The method performs early, systematic, online, automatic, permanent predictive supervision, and diagnosis of a high sampling frequency. It is based on the supervision of predictive electrical parameters easily accessible by the design of its architecture, whose detection and isolation precedes with an adequate margin of maneuver, to be able to alert and stop by means of automatic disconnection the degradation phenomenon and its cumulative effect causing the development of a future irrecoverable failure. Its architecture design is scalable and integrable in conventional photovoltaic installations. It emphasizes the use of low-cost technology such as the ESP8266 module, ASC712-5A, and FZ0430 sensors and relay modules. The method is based on data acquisition with the ESP8266 module, which is sent over the internet to the computer where a SCADA system (iFIX V6.5) is installed, using the Modbus TCP/IP and OPC communication protocols. Detection thresholds are initially obtained experimentally by applying inductive shading methods on specific solar panels.García Moreno, E.; Ponluisa, N.; Quiles Cucarella, E.; Zotovic Stanisic, R.; Gutiérrez, SC. (2022). Solar Panels String Predictive and Parametric Fault Diagnosis Using Low-Cost Sensors. Sensors. 22(1):1-29. https://doi.org/10.3390/s2201033212922

Entrenamiento en remediación cognitiva y habilidades emocionales en formato grupal para pacientes con obesidad: un estudio piloto

Resumen: El entrenamiento en remediación cognitiva y habilidades emocionales fue diseñado inicialmente por el grupo de Tchanturia para la intervención individual de pacientes con trastornos alimentarios. El objetivo del presente trabajo es exponer su adaptación a pacientes con obesidad en formato grupal. Se estructura en 8 sesiones semanales en las que se trabaja funciones de ejecutivo central, la relación entre emoción y pensamiento, y el manejo emocional en un mismo y en los demás. Además, presentamos los resultados preliminares de su aplicación a un grupo de 5 participantes con obesidad mórbida con el fin de valorar su posible eficacia. Los resultados indican que tras la intervención, las pacientes mostraron una mejoría en sus habilidades cognitivas y emocionales, así como en las conductas relacionadas con la alimentación y el peso. Este programa puede ser una intervención útil complemento del tratamiento de la obesidad en sus fases iniciales

The enterococcal surface protein, Esp, is involved in Enterococcus faecalis biofilm formation

The enterococcal surface protein, Esp, is a high-molecular-weight surface protein of unknown function whose frequency is significantly increased among infection-derived Enterococcus faecalis isolates. In this work, a global structural similarity was found between Bap, a biofilm-associated protein of Staphylococcus aureus, and Esp. Analysis of the relationship between the presence of the Esp-encoding gene (esp) and the biofilm formation capacity in E. faecalis demonstrated that the presence of the esp gene is highly associated (P < 0.0001) with the capacity of E. faecalis to form a biofilm on a polystyrene surface, since 93.5% of the E. faecalis esp-positive isolates were capable of forming a biofilm. Moreover, none of the E. faecalis esp-deficient isolates were biofilm producers. Depending on the E. faecalis isolate, insertional mutagenesis of esp caused either a complete loss of the biofilm formation phenotype or no apparent phenotypic defect. Complementation studies revealed that Esp expression in an E. faecalis esp-deficient strain promoted primary attachment and biofilm formation on polystyrene and polyvinyl chloride plastic from urine collection bags. Together, these results demonstrate that (i) biofilm formation capacity is widespread among clinical E. faecalis isolates, (ii) the biofilm formation capacity is restricted to the E. faecalis strains harboring esp, and (iii) Esp promotes primary attachment and biofilm formation of E. faecalis on abiotic surfaces

Comparison of methods for the detection of biofilm production in coagulase-negative staphylococci

<p>Abstract</p> <p>Background</p> <p>The ability of biofilm formation seems to play an essential role in the virulence of coagulase-negative staphylococci (CNS). The most clearly characterized component of staphylococcal biofilms is the polysaccharide intercellular adhesin (PIA) encoded by the <it>icaADBC </it>operon. Biofilm production was studied in 80 coagulase-negative staphylococci (CNS) strains isolated from clinical specimens of newborns with infection hospitalized at the Neonatal Unit of the University Hospital, Faculty of Medicine of Botucatu, and in 20 isolates obtained from the nares of healthy individuals without signs of infection. The objective was to compare three phenotypic methods with the detection of the <it>icaA</it>, <it>icaD </it>and <it>icaC </it>genes by PCR.</p> <p>Findings</p> <p>Among the 100 CNS isolates studied, 82% tested positive by PCR, 82% by the tube test, 81% by the TCP assay, and 73% by the CRA method. Using PCR as a reference, the tube test showed the best correlation with detection of the <it>ica </it>genes, presenting high sensitivity and specificity.</p> <p>Conclusions</p> <p>The tube adherence test can be indicated for the routine detection of biofilm production in CNS because of its easy application and low cost and because it guarantees reliable results with excellent sensitivity and specificity.</p

The Pleiotropic CymR Regulator of Staphylococcus aureus Plays an Important Role in Virulence and Stress Response

We have characterized a novel pleiotropic role for CymR, the master regulator of cysteine metabolism. We show here that CymR plays an important role both in stress response and virulence of Staphylococcus aureus. Genes involved in detoxification processes, including oxidative stress response and metal ion homeostasis, were differentially expressed in a ΔcymR mutant. Deletion of cymR resulted in increased sensitivity to hydrogen peroxide-, disulfide-, tellurite- and copper-induced stresses. Estimation of metabolite pools suggests that this heightened sensitivity could be the result of profound metabolic changes in the ΔcymR mutant, with an increase in the intracellular cysteine pool and hydrogen sulfide formation. Since resistance to oxidative stress within the host organism is important for pathogen survival, we investigated the role of CymR during the infectious process. Our results indicate that the deletion of cymR promotes survival of S. aureus inside macrophages, whereas virulence of the ΔcymR mutant is highly impaired in mice. These data indicate that CymR plays a major role in virulence and adaptation of S. aureus for survival within the host

FHA-Mediated Cell-Substrate and Cell-Cell Adhesions Are Critical for Bordetella pertussis Biofilm Formation on Abiotic Surfaces and in the Mouse Nose and the Trachea

Bordetella spp. form biofilms in the mouse nasopharynx, thereby providing a potential mechanism for establishing chronic infections in humans and animals. Filamentous hemagglutinin (FHA) is a major virulence factor of B. pertussis, the causative agent of the highly transmissible and infectious disease, pertussis. In this study, we dissected the role of FHA in the distinct biofilm developmental stages of B. pertussis on abiotic substrates and in the respiratory tract by employing a murine model of respiratory biofilms. Our results show that the lack of FHA reduced attachment and decreased accumulation of biofilm biomass on artificial surfaces. FHA contributes to biofilm development by promoting the formation of microcolonies. Absence of FHA from B. pertussis or antibody-mediated blockade of surface-associated FHA impaired the attachment of bacteria to the biofilm community. Exogenous addition of FHA resulted in a dose-dependent inhibitory effect on bacterial association with the biofilms. Furthermore, we show that FHA is important for the structural integrity of biofilms formed on the mouse nose and trachea. Together, these results strongly support the hypothesis that FHA promotes the formation and maintenance of biofilms by mediating cell-substrate and inter-bacterial adhesions. These discoveries highlight FHA as a key factor in establishing structured biofilm communities in the respiratory tract

Factors Contributing to the Biofilm-Deficient Phenotype of Staphylococcus aureus sarA Mutants

Mutation of sarA in Staphylococcus aureus results in a reduced capacity to form a biofilm, but the mechanistic basis for this remains unknown. Previous transcriptional profiling experiments identified a number of genes that are differentially expressed both in a biofilm and in a sarA mutant. This included genes involved in acid tolerance and the production of nucleolytic and proteolytic exoenzymes. Based on this we generated mutations in alsSD, nuc and sspA in the S. aureus clinical isolate UAMS-1 and its isogenic sarA mutant and assessed the impact on biofilm formation. Because expression of alsSD was increased in a biofilm but decreased in a sarA mutant, we also generated a plasmid construct that allowed expression of alsSD in a sarA mutant. Mutation of alsSD limited biofilm formation, but not to the degree observed with the corresponding sarA mutant, and restoration of alsSD expression did not restore the ability to form a biofilm. In contrast, concomitant mutation of sarA and nuc significantly enhanced biofilm formation by comparison to the sarA mutant. Although mutation of sspA had no significant impact on the ability of a sarA mutant to form a biofilm, a combination of protease inhibitors (E-64, 1-10-phenanthroline, and dichloroisocoumarin) that was shown to inhibit the production of multiple extracellular proteases without inhibiting growth was also shown to enhance the ability of a sarA mutant to form a biofilm. This effect was evident only when all three inhibitors were used concurrently. This suggests that the reduced capacity of a sarA mutant to form a biofilm involves extracellular proteases of all three classes (serine, cysteine and metalloproteases). Inclusion of protease inhibitors also enhanced biofilm formation in a sarA/nuc mutant, with the combined effect of mutating nuc and adding protease inhibitors resulting in a level of biofilm formation with the sarA mutant that approached that of the UAMS-1 parent strain. These results demonstrate that the inability of a sarA mutant to repress production of extracellular nuclease and multiple proteases have independent but cumulative effects that make a significant contribution to the biofilm-deficient phenotype of an S. aureus sarA mutant

agr-Mediated Dispersal of Staphylococcus aureus Biofilms

The agr quorum-sensing system of Staphylococcus aureus modulates the expression of virulence factors in response to autoinducing peptides (AIPs). Recent studies have suggested a role for the agr system in S. aureus biofilm development, as agr mutants exhibit a high propensity to form biofilms, and cells dispersing from a biofilm have been observed displaying an active agr system. Here, we report that repression of agr is necessary to form a biofilm and that reactivation of agr in established biofilms through AIP addition or glucose depletion triggers detachment. Inhibitory AIP molecules did not induce detachment and an agr mutant was non-responsive, indicating a dependence on a functional, active agr system for dispersal. Biofilm detachment occurred in multiple S. aureus strains possessing divergent agr systems, suggesting it is a general S. aureus phenomenon. Importantly, detachment also restored sensitivity of the dispersed cells to the antibiotic rifampicin. Proteinase K inhibited biofilm formation and dispersed established biofilms, suggesting agr-mediated detachment occurred in an ica-independent manner. Consistent with a protease-mediated mechanism, increased levels of serine proteases were detected in detaching biofilm effluents, and the serine protease inhibitor PMSF reduced the degree of agr-mediated detachment. Through genetic analysis, a double mutant in the agr-regulated Aur metalloprotease and the SplABCDEF serine proteases displayed minimal extracellular protease activity, improved biofilm formation, and a strongly attenuated detachment phenotype. These findings indicate that induction of the agr system in established S. aureus biofilms detaches cells and demonstrate that the dispersal mechanism requires extracellular protease activity