Fcgamma receptor-mediated phagocytosis in macrophages lacking the Src family tyrosine kinases Hck, Fgr, and Lyn.

Macrophage Fcgamma receptors (FcgammaRs) mediate the uptake and destruction of antibody-coated viruses, bacteria, and parasites. We examined FcgammaR signaling and phagocytic function in bone marrow-derived macrophages from mutant mice lacking the major Src family kinases expressed in these cells, Hck, Fgr, and Lyn. Many FcgammaR-induced functional responses and signaling events were diminished or delayed in these macrophages, including immunoglobulin (Ig)G-coated erythrocyte phagocytosis, respiratory burst, actin cup formation, and activation of Syk, phosphatidylinositol 3-kinase, and extracellular signal-regulated kinases 1 and 2. Significant reduction of IgG-dependent phagocytosis was not seen in hck(-)(/)-fgr(-)(/)- or lyn(-)(/)- cells, although the single mutant lyn(-)(/)- macrophages did manifest signaling defects. Thus, Src family kinases clearly have roles in two events leading to FcgammaR-mediated phagocytosis, one involving initiation of actin polymerization and the second involving activation of Syk and subsequent internalization. Since FcgammaR-mediated phagocytosis did occur at modest levels in a delayed fashion in triple mutant macrophages, these Src family kinases are not absolutely required for uptake of IgG-opsonized particles

Ambient Intelligence through Image Retrieval

An ambient intelligent environment needs dynamic enrollment of strangers without too much human intervention. For this purpose, we propose an entity recognition process based on images captured with low-cost but widespread webcams and easy-to-deploy image processing techniques. We find that the use of levels of confidence in recognition due to different techniques and context-based image retrieval improves the process

Application of a prioritisation scheme for seismic intervention in schools buildings in Italy

A risk management framework has recently been developed to assign priorities for the rehabilitation of school buildings in Italy, and to give timescales within which retrofit or demolition must take place. Since it is not practical to carry out a detailed assessment of the 60,000 Italian state and public schools, the framework is a multiple-level procedure which aims to identify the highest-risk buildings based on filters of increasing detail, and reduces the size of the building inventory at each step. The first risk ranking is based on a strength deficit, which measures the difference between the current design forces defined for the building site and an estimation of the level of seismic resistance which was required at the time of design. The second ranking is based on lateral strength calculations that are already available for a large portion of the Italian masonry building stock, and that are obtained from a survey form that is familiar to Italian engineers. Finally, a simplified displacement-based methodology is used to give a more accurate assessment of seismic risk based on a limited amount of geometrical and material data. The final assessment leads to a capacity ratio and a risk rating, which are used within a transparent procedure to assign priorities for seismic intervention, and timescales within which detailed assessment leading to retrofit or demolition must take place. The first step of the methodology has been applied herein to the school building stock within two Regions in Italy and preliminary results are presented

DSM-5 criteria for substance use disorders: recommendations and rationale.

Since DSM-IV was published in 1994, its approach to substance use disorders has come under scrutiny. Strengths were identified (notably, reliability and validity of dependence), but concerns have also arisen. The DSM-5 Substance-Related Disorders Work Group considered these issues and recommended revisions for DSM-5. General concerns included whether to retain the division into two main disorders (dependence and abuse), whether substance use disorder criteria should be added or removed, and whether an appropriate substance use disorder severity indicator could be identified. Specific issues included possible addition of withdrawal syndromes for several substances, alignment of nicotine criteria with those for other substances, addition of biomarkers, and inclusion of nonsubstance, behavioral addictions.This article presents the major issues and evidence considered by the work group, which included literature reviews and extensive new data analyses. The work group recommendations for DSM-5 revisions included combining abuse and dependence criteria into a single substance use disorder based on consistent findings from over 200,000 study participants, dropping legal problems and adding craving as criteria, adding cannabis and caffeine withdrawal syndromes, aligning tobacco use disorder criteria with other substance use disorders, and moving gambling disorders to the chapter formerly reserved for substance-related disorders. The proposed changes overcome many problems, while further studies will be needed to address issues for which less data were available

Electroless thin film CoNiFe-B alloys for integrated magnetics on Si

Electroless magnetic thin films have been deposited from borane-based baths suitable for use in integrated magnetics on Si applications. The baths were developed for compatibility with standard photoresist for microfabrication of integrated magnetics on Si. The specific formulations, which differ from those reported previously, yield uniform, high saturation magnetisation (up to 2.15 T) deposits with low coercivity (<2 Oe). The resistivity of the film can be increased to minimise eddy current losses by using higher dimethylamine borane (DMAB) content or the inclusion of a second reducing agent, hypophosphite, to facilitate phosphorus codeposition of up to 7 at.%. The Ni content in the plating bath has been shown to exert significant influence over the composition, deposition rate and coercivity. XRD analysis suggests that the deposits consist of nanocrystalline phase with grains <20 nm. Such small grains are consistent with the observed low coercivity of the deposits

A comparison of seismic risk maps for Italy

National seismic risk maps are an important risk mitigation tool as they can be used for the prioritization of regions within a country where retrofitting of the building stock or other risk mitigation measures should take place. The production of a seismic risk map involves the convolution of seismic hazard data, vulnerability predictions for the building stock and exposure data. The seismic risk maps produced in Italy over the past 10 years are compared in this paper with recent proposals for seismic risk maps based on state-of-the-art seismic hazard data and mechanics-based vulnerability assessment procedures. The aim of the paper is to open the discussion for the way in which future seismic risk maps could be produced, making use of the most up-to-date information in the fields of seismic hazard evaluation and vulnerability assessment

Application of a prioritisation scheme for seismic intervention in schools buildings in Italy

A risk management framework has recently been developed to assign priorities for the rehabilitation of school buildings in Italy, and to give timescales within which retrofit or demolition must take place. Since it is not practical to carry out a detailed assessment of the 60,000 Italian state and public schools, the framework is a multiple-level procedure which aims to identify the highest-risk buildings based on filters of increasing detail, and reduces the size of the building inventory at each step. The first risk ranking is based on a strength deficit, which measures the difference between the current design forces defined for the building site and an estimation of the level of seismic resistance which was required at the time of design. The second ranking is based on lateral strength calculations that are already available for a large portion of the Italian masonry building stock, and that are obtained from a survey form that is familiar to Italian engineers. Finally, a simplified displacement-based methodology is used to give a more accurate assessment of seismic risk based on a limited amount of geometrical and material data. The final assessment leads to a capacity ratio and a risk rating, which are used within a transparent procedure to assign priorities for seismic intervention, and timescales within which detailed assessment leading to retrofit or demolition must take place. The first step of the methodology has been applied herein to the school building stock within two Regions in Italy and preliminary results are presented

Exotic torus manifolds and equivariant smooth structures on quasitoric manifolds

In 2006 Masuda and Suh asked if two compact non-singular toric varieties having isomorphic cohomology rings are homeomorphic. In the first part of this paper we discuss this question for topological generalizations of toric varieties, so-called torus manifolds. For example we show that there are homotopy equivalent torus manifolds which are not homeomorphic. Moreover, we characterize those groups which appear as the fundamental groups of locally standard torus manifolds. In the second part we give a classification of quasitoric manifolds and certain six-dimensional torus manifolds up to equivariant diffeomorphism. In the third part we enumerate the number of conjugacy classes of tori in the diffeomorphism group of torus manifolds. For torus manifolds of dimension greater than six there are always infinitely many conjugacy classes. We give examples which show that this does not hold for six-dimensional torus manifolds.Comment: 21 pages, 2 figures, results about quasitoric manifolds adde