Genetic typing of Candida albicans strains isolated from the oral cavity of patients with denture stomatitis before and after itraconazole therapy

This study determined, by molecular typing of C. albicans species isolated from denture stomatitis patients with a mycological relapse six months after successful itraconazole therapy, whether there had been recurrence of infection with the same strain(s), selection of particular strains or infection with new strains of C. albicans. Forty patients with long-standing Candida-associated denture stomatitis were assigned either cyclodextrin itraconazole solution or itraconazole capsules (100mg b.d. for 15 days). Palatal erythema was measured and imprint cultures undertaken at baseline and at 15 days, four weeks and six months after treatment commenced. Yeast isolates were formally identified and chromosomal DNA was extracted from pairs of isolates from those patients with C. albicans present at baseline and six months after treatment commenced. Southern blotting of EcoRI-digested chromosomal DNA was performed using the C. albicans-specific 27A repetitive element as a probe. Eighteen of 36 patients were infected with C. albicans at baseline and six months after treatment commenced. Overall, 13 genetically different strains of C. albicans were found. However, in 17 of 18 patients, the C. albicans strains isolated prior to itraconazole therapy and six months later were the same. Thus recurrence of denture stomatitis in these individuals was due to re-colonisation by the original strain, rather than re-infection with a different strain. Key words: Genotyping, C. albicans, denture stomatitis

The oral microbiome of denture wearers is influenced by natural dentition

Objectives: The composition of dental plaque has been well defined, whereas currently there is limited understanding of the composition of denture plaque and how it directly influences denture related stomatitis (DS). The aims of this study were to compare the microbiomes of denture wearers, and to understand the implications of these towards inter-kingdom and host-pathogen interactions within the oral cavity. Methods: Swab samples were obtained from 123 participants wearing either a complete or partial denture; the bacterial composition of each sample was determined using bar-coded illumina MiSeq sequencing of the bacterial hypervariable V4 region of 16S rDNA. Sequencing data processing was undertaken using QIIME, clustered in Operational Taxonomic Units (OTUs) and assigned to taxonomy. The dentures were sonicated to remove the microbial flora residing on the prosthesis, sonicate was then cultured using diagnostic colorex Candida media. Samples of unstimulated saliva were obtained and antimicrobial peptides (AMP) levels were measured by ELISA. Results: We have shown that dental and denture plaques are significantly distinct both in composition and diversity and that the oral microbiome composition of a denture wearer is variable and is influenced by the location within the mouth. Dentures and mucosa were predominantly made up of Bacilli and Actinobacteria. Moreover, the presence of natural teeth has a significant impact on the overall microbial composition, when compared to the fully edentulous. Furthermore, increasing levels of Candida spp. positively correlate with Lactobacillus spp. AMPs were quantified, though showed no specific correlations. Conclusions: This is the first study to provide a detailed understanding of the oral microbiome of denture wearers and has provided evidence that DS development is more complex than simply a candidal infection. Both fungal and bacterial kingdoms clearly play a role in defining the progression of DS, though we were unable to show a defined role for AMPs

Exploring the Educational Involvement of Parents of English Learners

The purpose of the current investigation was to examine the relationships among a range of specific barriers and facilitators of parent involvement and a variety of types of school involvement within a diverse group of immigrant parents of English Learners (ELs) in four elementary school districts. In-home types of educational involvement such as monitoring homework and asking children about their school day were the most commonly reported behaviors, and utilizing community resources was found to be the least common type of parental involvement. Involvement type was predicted by parental demographic factors such as comfort with English language, educational background, and ethnicity, as well as perceptions of barriers and overall school climate. The findings of this study have implications for the design and implementation of interventions (e.g., parent programs, school policy changes) aimed at increasing the parental involvement of EL children

Preliminary validity and reliability evidence of the Brief Antisocial Behavior Scale (B-ABS) in young adults from four countries

The present research built on the Self-Reported Delinquency interview and the Antisocial Behavior Scale to develop an updated brief instrument to measure antisocial behavior. College students (n = 3188, 67.75% women) from the USA, Argentina, the Netherlands and Spain completed an online survey. Analyses that combined approaches from the Classical Test Theory and Item Response Theory were conducted to select the items for the brief version. Findings suggested that a 13-item Brief Antisocial Behavior Scale (B-ABS) fulfilled the high-quality criteria: salient factor loadings, adequate discrimination, variability in response endorsement, adequate fit based on infit/outfit values, nondifferent item functioning across the four participating countries, and Cronbach?s alpha and ordinal omega coefficients higher than .70. The B-ABS scores generally significantly correlated with personality scores, mental health and marijuana outcomes, showing criterion-related validity evidence. Our overall findings suggest that B-ABS adequately assesses antisocial behavior in young adults from different countries/cultures.Fil: Mezquita, Laura. Universitat Jaume I; EspañaFil: Bravo, Adrian J.. College of William and Mary; Estados UnidosFil: Pilatti, Angelina. Universidad Nacional de Córdoba. Instituto de Investigaciones Psicológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Psicológicas; ArgentinaFil: Ortet, Generós. Universitat Jaume I; EspañaFil: Ibáñez, Manuel I.. Universitat Jaume I; Españ

ENDO-Pore:high-throughput linked-end mapping of single DNA cleavage events using nanopore sequencing

Mapping the precise position of DNA cleavage events plays a key role in determining the mechanism and function of endonucleases. ENDO-Pore is a high-throughput nanopore-based method that allows the time resolved mapping single molecule DNA cleavage events in vitro. Following linearisation of a circular DNA substrate by the endonuclease, a resistance cassette is ligated recording the position of the cleavage event. A library of single cleavage events is constructed and subjected to rolling circle amplification to generate concatemers. These are sequenced and used to produce accurate consensus sequences. To identify the cleavage site(s), we developed CSI (Cleavage Site Investigator). CSI recognizes the ends of the cassette ligated into the cleaved substrate and triangulates the position of the dsDNA break. We firstly benchmarked ENDO-Pore using Type II restriction endonucleases. Secondly, we analysed the effect of crRNA length on the cleavage pattern of CRISPR Cas12a. Finally, we mapped the time-resolved DNA cleavage by the Type ISP restriction endonuclease LlaGI that introduces random double-strand breaks into its DNA substrates

Young adult concurrent use and simultaneous use of alcohol and marijuana: A cross-national examination among college students in seven countries

Introduction: Many young adults report frequent co-use of alcohol and marijuana, with some individuals engaging in simultaneous use (SAM; use of both substances within the same occasion resulting in an overlap of their effects) and others in concurrent use (CAM; use of both substances during a similar time period [e.g., past 30 days] but not within the same occasion). Emerging work demonstrates that SAM relative to CAM use places individuals at a greater risk for substance-related harms; however, these results primarily rely on U.S. samples. The goal of the present multi-country study was to examine prevalence rates of CAM and SAM use and examine differences in past 30-day SAM/CAM use on alcohol/marijuana substance-related outcomes among college students from seven countries. Methods: A total of 9171 (70.5% women; Mean age = 20.28, SD = 3.96) college students participated in the cross-sectional online survey study. Results: Among students who endorsed use of both alcohol and marijuana in the past 30-days (n = 2124), SAM use (75.8%) was far more prevalent than CAM use (24.2%). Moreover, ∼75% of students endorsed SAM use within each country subsample. Regression models showed that SAM vs. CAM use was associated with greater alcohol and marijuana use and negative consequences. Conclusions: College students from around the world endorse high rates of SAM use, and this pattern of co-use is associated with greater frequency of use and substance-related harms. On college campuses, SAM use should be a target of clinical prevention/intervention efforts and the mechanisms underpinning the unique harms of SAM need to be clarified.Fil: Bravo, Adrian J.. College of William and Mary; Estados UnidosFil: Prince, Mark A.. State University of Colorado at Boulder; Estados UnidosFil: Pilatti, Angelina. Universidad Nacional de Córdoba. Instituto de Investigaciones Psicológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Psicológicas; ArgentinaFil: Mezquita, Laura. Universitat Jaume I; EspañaFil: Keough, Matthew T.. University of York; Reino UnidoFil: Hogarth, Lee. University of Exeter; Reino Unid

Speaking out about gender imbalance in invited speakers improves diversity.

Omissions of qualified women scientists from major meeting programs continue to occur despite a surge in articles indicating persistent gender-discriminatory practices in hiring and promotion, and calls for gender balance in conference organizing committees

Motives to play videogames across seven countries: Measurement invariance of the Videogaming Motives Questionnaire

Introduction. Gaming motives appear to be an important predictor of time spent gaming and disordered gaming. The Videogaming Motives Questionnaire (VMQ) has shown adequate psychometric properties to assess gaming motives among Spanish college students. However, the utility of this measure has not yet been explored in other cultures. This research aimed to examine the structure and measurement invariance of the VMQ across seven countries and gender groups, and to provide criterion-related validity evidence for VMQ scores. Method. College students who reported having played videogames in the last year (n = 5192; 59.07 % women) from the US, Canada, South Africa, Spain, Argentina, England, and Uruguay completed an online survey to measure time spent gaming, disordered gaming, and the VMQ. Results. Findings support a 24-item 8-intercorrelated factor model structure for the VMQ in the total sample. Our results also support configural, metric, and scalar invariance of the VMQ across gender groups and countries. Students from North America (US and Canada) scored higher on most gaming motives (except recreation and cognitive development) than students from the other countries. The correlations between VMQ and non-VMQ variables were similar across gender and countries, except in England where VMQ correlations with time spent gaming were stronger. Discussion. These results suggest that the VMQ is a useful measure for assessing gaming motives across young adults from different countries.Funding for open access charge: CRUE-Universitat Jaume

Cross-cultural examination of the Big Five Personality Trait Short Questionnaire: Measurement invariance testing and associations with mental health

The present study examined the measurement invariance of the Big Five Personality Trait Short Questionnaire (BFPTSQ) across language (Spanish and English), Spanish-speaking country of origin (Argentina and Spain) and gender groups (female and male). Evidence of criterion-related validity was examined via associations (i.e., correlations) between the BFPTSQ domains and a wide variety of mental health outcomes. College students (n = 2158) from the USA (n = 1117 [63.21% female]), Argentina (n = 353 [65.72% female]) and Spain (n = 688 [66.86% female]) completed an online survey. Of the tested models, an Exploratory Structural Equation Model (ESEM) fit the data best. Multigroup ESEM and ESEM-within-CFA generally supported the measurement invariance of the questionnaire across groups. Internalizing symptomatology, rumination and low happiness were related mainly to low emotional stability across countries, while low agreeableness and low conscientiousness were related chiefly to externalizing symptomology (i.e., antisocial behavior and drug outcomes). Some correlational differences arose across countries and are discussed. Our findings generally support the BFPTSQ as an adequate measure to assess the Big Five personality domains in Spanish- and English-speaking young adults