The vertical datum problem

Het vertical datum problem is een, opnieuw actueel geworden, probleem sinds er binnen afzienbare tijd meer en nauwkeurigere data verwacht kunnen worden. Om daarmee zinvol te kunnen werken is ook een beter bepaalde referentie noodzakelijk. Daar wordt dan ook al druk naar gezocht . Het onderzoek van het vertical datum problem was gebaseerd op literatuuronderzoek; zowel voor het algemene deel als voor de bestudering van het (N)AP. Hoewel voor dit laatste deel ook de beide bezoeken aan de Meetkundige Dienst van de Rijks Waterstaat de nodige informatie leverden. (Zie hiervoor ook de bijlagen.)Civil Engineering and Geoscience

Pharmacokinetic Enhancement of HIV Antiretroviral Therapy During Pregnancy

Pharmacokinetic boosting of antiretroviral (ARV) therapies with either ritonavir or cobicistat is used to achieve target drug exposure, lower pill burden, and provide simplified dosing schedules. Several ARVs require boosting, including the integrase inhibitor elvitegravir as well as protease inhibitors such as darunavir, atazanavir, and lopinavir. The use of boosted regimens in pregnant women living with HIV has been studied for a variety of ARVs; however, a recent recommendation by the US Food and Drug Administration advised against cobicistat-boosted regimens in pregnancy due to substantially lower drug exposures observed in clinical pharmacokinetic studies. The objectives of this article are to review pharmacokinetic enhancement of ARVs with ritonavir and cobicistat during pregnancy and postpartum, describe clinical implications, and provide recommendations for future research

A mass spectrometry based imaging method developed for the intracellular detection of HIV protease inhibitors

Mass spectrometry imaging is a promising technique for measuring drugs and drug metabolites in cells and tissues. In this manuscript we describe a method for the imaging of HIV protease inhibitors. As a model system we used Mono Mac 6 cells cultured with the HIV protease inhibitors saquinavir and nelfinavir deposited on glass slides using a cytocentrifuge. A sublimation/deposition device for homogeneous matrix deposition was constructed which allows imaging of these HIV protease inhibitors at clinically relevant concentrations. Using this matrix sublimation/deposition method, glass slides containing the cytocentrifuged cells can be measured and analyzed by two types of mass spectrometry techniques, viz. matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) and MALDI Fourier transform ion cyclotron resonance (FTICR), and this makes it possible to perform imaging rapidly (MALDI-TOF) and with a very high selectivity (MALDI-FTICR). Copyright (C) 2009 John Wiley & Sons, Ltd