Anticuerpos a la carta combinando expresión in vitro de proteínas, tecnología de despliegue en fagos y arrays de anticuerpos

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Signaling alterations and cytokine profiles in late metastatic colorectal cancer promote an antiinflammatory profile and proliferation enhacement

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Evaluation of an O2-Substituted (1–3)-β-D-Glucan, Produced by Pediococcus parvulus 2.6, in ex vivo Models of Crohn’s Disease

Efecte antiinflamatori de la malaltia de Crohn; Immunomodulació; Exopolisacàrids bacteriansEfecto antiinflamatorio de la enfermedad de Crohn; Inmunomodulacion; Exopolisacáridos bacterianosCrohn's disease anti-inflammatory effect; Immunomodulation; Bacterial exopolysaccharides1,3-β-glucans are extracellular polysaccharides synthesized by microorganisms and plants, with therapeutic potential. Among them, the O2-substituted-(1–3)-β-D-glucan, synthesized by some lactic acid bacteria (LAB), has a prebiotic effect on probiotic strains, an immunomodulatory effect on monocyte-derived macrophages, and potentiates the ability of the producer strain to adhere to Caco-2 cells differentiated to enterocytes. In this work, the O2-substituted-(1–3)-β-D-glucan polymers produced by GTF glycoyltransferase in the natural host Pediococcus parvulus 2.6 and in the recombinant strain Lactococcus lactis NZ9000[pNGTF] were tested. Their immunomodulatory activity was investigated in an ex vivo model using human biopsies from patients affected by Crohn’s disease (CD). Both polymers had an anti-inflammatory effect including, a reduction of Interleukine 8 both at the level of its gene expression and its secreted levels. The overall data indicate that the O2-substituted-(1–3)-β-D-glucan have a potential role in ameliorating inflammation via the gut immune system cell modulation.This work was supported by the Spanish Ministry of Science, Innovation, and Universities (grant RTI2018-097114-B-I00), by the Fondo de Investigación Sanitaria, grant number PI12/00263, and by CIBERehd

Diets and trophic structure of fish assemblages in a large and unexplored subtropical river: The Uruguay river

This article belongs to the Special Issue Trophic Interactions in Warm Freshwater EcosystemsThe Neotropics represent a hotspot for freshwater biodiversity with vast number of fish species of scarce ecological knowledge. This holds true for the Uruguay River, where fish assemblages and their diets remain unexplored. Fish assemblages were surveyed in 14 sites along the river main course, from headwaters to mouth (approximately 1800 km), with the aim to identify the trophic roles of fishes and to describe trophic structure of these assemblages, following standardized sampling campaigns and laboratory procedures. One hundred species (2309 gut contents) were analysed and classified into four trophic groups subdivided into eight lower-level groups: Piscivore, piscivore-invertivore, detritivore, omnivore-detritivore, omnivore-invertivore, omnivore-planktivore and omnivore-herbivore. The trophic structure of the assemblages varied along the river, with the relative species richness of fish consuming terrestrial invertebrates increasing towards the middle river section, probably driven by the large floodplains in that areas, supporting global theories such as the flood pulse concept. This study describes the feeding habits of fish along the Uruguay River, being the first dietary description for 29 species. This knowledge is essential for management and conservation, serving as baseline in the context of future environmental changes while generating novel evidence on the functioning of ecosystems in this scarcely studied climatic region

Proteomic analysis of 1α,25-Dihydroxyvitamin D3 action on human colon cancer cells reveals a link to splicing regulation

14 páginas, 6 figuras, 2 tablas.El pdf del artículo es la versión pre-print.1α,25-Dihydroxyvitamin D3 (1,25(OH)2D3) and other vitamin D compounds are promising molecules in the prevention and therapy of colon cancer and other neoplasias. To study the mechanism of action of 1,25(OH)2D3 in colon cancer cells, we carried out a comparative proteomic analysis of the nuclear fractions of SW480-ADH cells treated with 1,25(OH)2D3 or vehicle during 8 or 48 h. 2D-DIGE analysis combined with MALDI-TOF-TOF mass spectrometry interrogation led to the identification of 59 differentially expressed unique proteins. Most identified proteins were nuclear, but several cytoskeleton-associated proteins were also detected. A good concordance between changes in expression at protein and RNA levels was observed for the validated proteins. A large group of identified proteins, such as SFPQ, SMARCE, KHSRP, TARDBP and PARP1, were involved in RNA processing or modification and have been ascribed to the spliceosome compartment of human cells. In addition, a smaller group of proteins (ERM (Ezrin, Radixin, Moesin) family, VCL, CORO1C, ACTB) were cytoskeleton-associated and played a role in cell adhesion and morphology. These results confirm the induction of epithelial phenotype by 1,25(OH)2D3 and suggest a role for vitamin D compounds in the regulation of the spliceosome and thus, in alternative splicing and possibly microRNA synthesis in colon cancer cells.Graphical abstract Proteomic analysis of 1α,25-Dihydroxyvitamin D3 action on human colon cancer cells reveals a link to splicing regulation. View high quality image (147K)This research was supported by grants from Colomics Programme Comunidad de Madrid (S-GEN-0266/2006), Ayudas a grupos estables de investigación Fundación Científica de la Asociación Española contra el Cáncer to J.I.C., Ministerio de Ciencia e Innovación de España (BIO2009-08818 (J.I.C.), SAF07-60341(A.M.) and SAF2010-18302 (A.M.)), Proteored platform (J.I.C.), and Red Temática de Investigación Cooperativa en Cáncer (RD06/0020/0009, ISCIII) (A.M.)Peer reviewe

Role of PSD-95 inhibitors in stroke and neuroprotection: A systematic review on NA-1 (Tat-NR2B9c)

Stroke is still the second cause of death and the first cause of acquired adult disability worldwide. Due to strict inclusion criteria, thrombolysis with tissue plasminogen activator (tPA), the only approved stroke treatment, is given to few patients. Therefore, new treatments are urgently needed. Excitotoxicity, relevant to secondary neuronal death in ischemic penumbra, has become a popular stroke target. Recently, administration of a peptide -Tat-NR2B9c, or NA-1- that prevents the interaction of the ionotropic glutamate receptor subunit GluN2B (formerly NR2B) with the postsynaptic density protein 95 (PSD-95) scaffold protein showed promising results. A systematic analysis of all identified preclinical studies using NA-1 in stroke models was conducted, and translational strategies to use the agent in human stroke were described and discussed. Seven studies involving 359 animals (260 rats, 33 mice and 66 macaques; corresponding, respectively, to 189 control- and 170 NA-1-treated animals) showed a significant 42.8% reduction in infarct size in the NA-1-treated group. However, funnel plot analysis demonstrated a publication bias which decreased protection to 17.6% after correction. Overall, the quality score of those studies was acceptable (6; interquartile range = 5-9), although no study used comorbid animals. Interestingly, successful translation to human iatrogenic stroke during aneurysm repair make NA-1 a strong candidate. NA-1 has successfully advanced from preclinical to human brain ischemia trials but still needs to fulfill some important STAIR requirements to become an ideal neuroprotectant.MC has received a predoctoral fellowship from the Instituto de Salud Carlos III and DG is a statistical fellow funded by the Spanish stroke network (INVICTUS). MD-G is supported by grant BFU2010-18380/BFI from the Ministerio de Economía y Competitividad.Peer Reviewe

Identificación de péptidos específicos de cáncer colorrectal mediante el uso de librerías de fagos T7 impresas en microarrays

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Regulation of epithelial-mesenchymal transition in colo cancer by 1α,25-dyhidroxyvitamin D: a proteomics approach

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Evaluation of an O 2-Substituted (1-3)-β-D-Glucan, Produced by Pediococcus parvulus 2.6, in ex vivo Models of Crohn's Disease

1,3-β-glucans are extracellular polysaccharides synthesized by microorganisms and plants, with therapeutic potential. Among them, the O 2-substituted-(1-3)-β-D-glucan, synthesized by some lactic acid bacteria (LAB), has a prebiotic effect on probiotic strains, an immunomodulatory effect on monocyte-derived macrophages, and potentiates the ability of the producer strain to adhere to Caco-2 cells differentiated to enterocytes. In this work, the O 2-substituted-(1-3)-β-D-glucan polymers produced by GTF glycoyltransferase in the natural host Pediococcus parvulus 2.6 and in the recombinant strain Lactococcus lactis NZ9000[pNGTF] were tested. Their immunomodulatory activity was investigated in an ex vivo model using human biopsies from patients affected by Crohn's disease (CD). Both polymers had an anti-inflammatory effect including, a reduction of Interleukine 8 both at the level of its gene expression and its secreted levels. The overall data indicate that the O 2-substituted-(1-3)-β-D-glucan have a potential role in ameliorating inflammation via the gut immune system cell modulatio