A New Genus and Species of Cryptogonimid from Lutjanus Spp. (Pisces: Lutjanidae) on the Great Barrier Reef and New Caledonia

Lobosorchis tibaldiae n. gen. and n. sp. (Digenea: Cryptogonimidae) is described from the intestine, pyloric ceca, and rectum of 2 species of Lutjanus (Pisces: Lutjanidae), Lutjanus carponotatus and Lutjanus fulviflamma, from the Great Barrier Reef, Queensland, Australia, and New Caledonia. The genus is tentatively placed in the Neochasminae and is distinguished within the Cryptogonimidae by the combination of follicular testes, oral spines, and vitelline follicles restricted to the anterior region of the body not extending posteriorly to the ventral sucker

Stephanostomum spp. (Digenea: Acanthocolpidae)from scombrids and carangids (Perciformes) from the Great Barrier Reef, with the description of two new species

Two new species and 4 Stephanostomum spp. as new host and/or locality records from Percifomes from the Great Barrier Reef are described: Stephanostomum lamothei n. sp. from Grammatorcymus bilineatus (type-host) and G. bicarinatus, Lizard Island and Swain Reefs, is characterised by its 50-55 circum-oral spines and >than 20% of the hindbody length Jacking vitelline follicles; Stephanostomum tupatupa n. sp. from Caranx papuensis, Lizard Island, is characterised by its 34-36 circum-oral spines an

Three members of Opisthomonorcheides Parukhin, 1966 (Digenea: Monorchiidae) from carangid fishes (Perciformes) from Indonesia, with a review of the genus

Three species of Opisthomonorcheides Parukhin, 1966 are reported for the first time from Indonesian waters: O. pampi (Wang, 1982) Liu, Peng, Gao, Fu, Wu, Lu, Gao & Xiao, 2010 and O. ovacutus (Mamaev, 1970) Machida, 2011 from Parastromateus niger (Bloch), and O. decapteri Parukhin, 1966 from Atule mate (Cuvier). Both O. pampi and O. ovacutus can now be considered widespread in the Indo-Pacific region, with earlier records of these species being from Fujian Province, China and Penang, Malaysia, respectively. We redescribe O. decapteri from one of its original hosts, Atule mate, off New Caledonia, and report this species from Jakarta Bay, Indonesia, extending its range throughout the Indian Ocean into the south-western Pacific. All three species possess a genital atrium that is long, sometimes very long, and a genital pore that is located in the forebody. This validates the interpretation that the original description was erroneous in reporting the genital pore in the hindbody, well posterior to the ventral sucker. These observations verify the synonymy of Retractomonorchis Madhavi, 1977 with Opisthomonorcheides. A major discrepancy between the species of Opisthomonorcheides is that some are described with the uterus entering the terminal organ laterally and some with it entering terminally; this feature needs further analysis. Based on the length of the genital atrium and the posterior extent of the vitellarium, the 27 species of Opisthomonorcheides considered valid can be divided into four groups. Among the 53 host records analysed, the families Carangidae (53% of records), Stromateidae (17%) and Serranidae (5.7%) are the most common; the reports are overwhelmingly from members of the Perciformes (91%), with further records in the Clupeiformes (5.7%), Gadiformes (1.9%) and Pleuronectiformes (1.9%). Two fish genera (Parastromateus Bleeker and Pampus Bonaparte) dominate the recorded hosts, with the black pomfret Parastromateus niger harbouring six species, the silver pomfret Pampus argenteus (Euphrasen) harbouring six, and the Chinese silver pomfret P. chinensis (Euphrasen) two. A host-parasite checklist is presented. We discuss the host-specificity of members of the genus, questioning some records such as that of O. decapteri in a deep-sea macrourid. We also comment on the morphological similarity, but phylogenetic distance, between the various Pomfret species, advancing the possibility that a series of host misidentifications has occurred. Sequences of the ITS2 rDNA gene generated for O. pampi and O. ovacutus are briefly discussed and molecular data are lodged in the GenBank database

High-intensity cardiac infections of Phthinomita heinigerae n. sp. (Digenea: Aporocotylidae) in the orangelined cardinalfish, Taeniamia fucata (Cantor), off Heron Island on the Great Barrier Reef.

We report a new species of aporocotylid trematode (Platyhelminthes: Digenea) from the heart of the orangelined cardinalfish, Taeniamia fucata (Cantor), from off Heron Island on the southern Great Barrier Reef. We used an integrated approach, analysing host distribution, morphology, and genetic data from the internal transcribed spacer 2 of the ribosomal DNA, to circumscribe Phthinomita heinigerae n. sp. This is the first species of Phthinomita Nolan & Cribb, 2006 reported from the Apogonidae; existing species and known 'types' are recorded from species of the Labridae, Mullidae, and Siganidae. The new species is distinguished from its 11 congeners in having a body 2977-3539 long and 16.5-22.4 times longer than wide, an anterior testis 6.2-8.2 times longer than wide and 8.3-13.0 times longer than the posterior testis, a posterior testis whose width is 35-56% of the body width, and an ovary positioned 11-13% of the body length from the posterior end, and is entirely anterior to the posterior margin of the anterior testis. In addition, 2-34 base differences (0.4-7.0% sequence divergence over 485 base positions) were detected among the ITS2 sequence representing P. heinigerae n. sp. and the 14 representing other Phthinomita species/molecular types. Prevalence and intensity of infection with P. heinigerae n. sp. was relatively high within the heart tissue of T. fucata, with 19 of 20 fish examined from off Heron Island infected (95%) with 7-25 adult worms (arithmetic mean 16.6). Infections by these parasites accounted for an occupation of 7-30% of the total estimated heart volume.This is the author accepted manuscript. The final version is available from Elsevier via http://dx.doi.org/10.1016/j.parint.2016.05.00

Trematodes of the Great Barrier Reef, Australia: emerging patterns of diversity and richness in coral reef fishes

The Great Barrier Reef holds the richest array of marine life found anywhere in Australia, including a diverse and fascinating parasite fauna. Members of one group, the trematodes, occur as sexually mature adult worms in almost all Great Barrier Reef bony fish species. Although the first reports of these parasites were made 100 years ago, the fauna has been studied systematically for only the last 25 years. When the fauna was last reviewed in 1994 there were 94 species known from the Great Barrier Reef and it was predicted that there might be 2,270 in total. There are now 326 species reported for the region, suggesting that we are in a much improved position to make an accurate prediction of true trematode richness. Here we review the current state of knowledge of the fauna and the ways in which our understanding of this fascinating group is changing. Our best estimate of the true richness is now a range, 1,100–1,800 species. However there remains considerable scope for even these figures to be incorrect given that fewer than one-third of the fish species of the region have been examined for trematodes. Our goal is a comprehensive characterisation of this fauna, and we outline what work needs to be done to achieve this and discuss whether this goal is practically achievable or philosophically justifiable

Molecular systematics of the digenean community parasitising the cerithiid gastropod Clypeomorus batillariaeformis Habe & Kusage on the Great Barrier Reef

A rich fauna of digenetic trematodes has been documented from the Great Barrier Reef (GBR), yet little is known of the complex life-cycles of these parasites which occur in this diverse marine ecosystem. At Heron Island, a small coral cay at the southern end of the GBR, the intertidal marine gastropod Clypeomorus batillariaeformis Habe & Kusage (Cerithiidae) is especially abundant. This gastropod serves as an intermediate host for 12 trematode species utilising both fish and avian definitive hosts. However, 11 of these species have been characterised solely with morphological data. Between 2015 and 2018 we collected 4870C. batillariaeformis from Heron Island to recollect these species with the goal of using molecular data to resolve their phylogenetic placement. We found eight of the 12 previously known species and two new forms, bringing the total number of digenean species known to parasitise C. batillariaeformis to 14. The families of this trematode community now include the Atractotrematidae Yamaguti, 1939, Bivesiculidae Yamaguti, 1934, Cyathocotylidae Mühling, 1898, Hemiuridae Looss, 1899, Heterophyidae Leiper, 1909, Himasthlidae Odhner, 1910, Microphallidae Ward, 1901, and Renicolidae Dollfus, 1939. Molecular data (ITS and 28S rDNA) were generated for all trematode species, and the phylogenetic position of each species was determined. The digenean community parasitising C. batillariaeformis includes several common species, as well as multiple species which are uncommon to rare. Although most of those trematodes in the community which exploit fishes as definitive hosts have remained common, the composition of those which utilise birds appears to have shifted over time

Dollfustrema durum n. sp. and Heterobucephalopsis perardua n. sp. (Digenea: Bucephalidae) from the giant moray eel, Gymnothorax javanicus (Bleeker) (Anguilliformes: Muraenidae), and proposal of the Heterobucephalopsinae n. subfam.

Two new species of bucephalid trematode (Platyhelminthes: Digenea) are described from the giant moray eel, Gymnothorax javanicus (Anguilliformes: Muraenidae), from off Lizard Island, Australia. We used a combined morphological and molecular-based approach targeting the internal transcribed spacer 2 (ITS2) of the ribosomal DNA (rDNA) and the D1-D3 region of the large subunit (28S) of rDNA to circumscribe the species. Dollfustrema durum n. sp. is distinguished from seven congeners in having 5-6 rows of enlarged body spines circling the anterior portion of the rhynchus. From the remaining 10 species, D. durum n. sp. differs in body length, and in having a caecum that terminates posteriorly to the confluent arc formed by the vitelline follicles, gonads predominantly anterior to the pharynx, testes in tandem, an anterior testis positioned posteriorly to the vitelline follicles, and the pre-vitelline field 23-40% of the body length. Heterobucephalopsis perardua n. sp. differs from Heterobucephalopsis gymnothoracis, the type- and only other reported species, in being two to three times smaller. Heterobucephalopsis, currently considered a genus inquirendum, is confirmed as valid and is rediagnosed. Bayesian inference analysis of 28S rDNA sequences representing 28 species from nine genera and four subfamilies of bucephalid, indicates that i) subfamily classifications previously based on morphological characters are broadly robust, ii) the sequence representing H. perardua n. sp. is resolved as distinct, and basal, to sequences representing the Bucephalinae, the Prosorhynchinae, the Paurorhynchinae, and the Dolichoenterinae, iii) the Dolichoenterinae and the Prosorhynchinae are monophyletic sister clades, basal to the Bucephalinae and the Paurorhynchinae, iv) sequences representing Grammatorcynicola, Prosorhynchus, and Dollfustrema are also monophyletic, v) the Bucephalinae is paraphyletic relative to the Paurorhynchinae, and vi) the bucephaline genera Prosorhynchoides, Rhipidocotyle, and Bucephalus are each polyphyletic. The morphological and molecular differences observed among the four previously recognised subfamilies in this study lead us to propose Heterobucephalopsinae n. subfam. to accommodate the genus Heterobucephalopsis.Continued funding from the Australian Research Council and the Australian Biological Research Study to THC supported this study.This is the author accepted manuscript. The final version is available from Elsevier via http://dx.doi.org/10.1016/j.parint.2015.07.00

\u3ci\u3eDollfustrema durum\u3c/i\u3e n. sp. and \u3ci\u3eHeterobucephalopsis perardua\u3c/i\u3e n. sp. (Digenea: Bucephalidae) from the giant moray eel, \u3ci\u3eGymnothorax javanicus\u3c/i\u3e (Bleeker) (Anguilliformes: Muraenidae), and proposal of the Heterobucephalopsinae n. subfam

Two new species of bucephalid trematode (Platyhelminthes: Digenea) are described from the giant moray eel, Gymnothorax javanicus (Anguilliformes: Muraenidae), from off Lizard Island, Australia. We used a combined morphological and molecular-based approach targeting the internal transcribed spacer 2 (ITS2) of the ribosomal DNA (rDNA) and the D1–D3 region of the large subunit (28S) of rDNA to circumscribe the species. Dollfustrema durum n. sp. is distinguished from seven congeners in having 5–6 rows of enlarged body spines circling the anterior portion of the rhynchus. From the remaining 10 species, D. durum n. sp. differs in body length, and in having a caecum that terminates posteriorly to the confluent arc formed by the vitelline follicles, gonads predominantly anterior to the pharynx, testes in tandem, an anterior testis positioned posteriorly to the vitelline follicles, and the pre-vitelline field 23–40% of the body length. Heterobucephalopsis perardua n. sp. differs from Heterobucephalopsis gymnothoracis, the type- and only other reported species, in being two to three times smaller. Heterobucephalopsis, currently considered a genus inquirendum, is confirmed as valid and is rediagnosed. Bayesian inference analysis of 28S rDNA sequences representing 28 species from nine genera and four subfamilies of bucephalid, indicates that i) subfamily classifications previously based on morphological characters are broadly robust, ii) the sequence representing H. perardua n. sp. is resolved as distinct, and basal, to sequences representing the Bucephalinae, the Prosorhynchinae, the Paurorhynchinae, and the Dolichoenterinae, iii) the Dolichoenterinae and the Prosorhynchinae are monophyletic sister clades, basal to the Bucephalinae and the Paurorhynchinae, iv) sequences representing Grammatorcynicola, Prosorhynchus, and Dollfustrema are also monophyletic, v) the Bucephalinae is paraphyletic relative to the Paurorhynchinae, and vi) the bucephaline genera Prosorhynchoides, Rhipidocotyle, and Bucephalus are each polyphyletic. The morphological and molecular differences observed among the four previously recognised subfamilies in this study lead us to propose Heterobucephalopsinae n. subfam. to accommodate the genus Heterobucephalopsis