Nitrate and ammonium lead to distinct global dynamic phosphorylation patterns when resupplied to nitrogen-starved Arabidopsis seedlings

Nitrogen is an essential macronutrient for plant growth and development. Inorganic nitrogen and its assimilation products control various metabolic, physiological and developmental processes. Although the transcriptional responses induced by nitrogen have been extensively studied in the past, our work here focused on the discovery of candidate proteins for regulatory events that are complementary to transcriptional changes. Most signaling pathways involve modulation of protein abundance and/or activity by protein phosphorylation. Therefore, we analyzed the dynamic changes in protein phosphorylation in membrane and soluble proteins from plants exposed to rapid changes in nutrient availability over a time course of 30 min. Plants were starved of nitrogen and subsequently resupplied with nitrogen in the form of nitrate or ammonium. Proteins with maximum change in their phosphorylation level at up to 5 min after nitrogen resupply (fast responses) included GPI-anchored proteins, receptor kinases and transcription factors, while proteins with maximum change in their phosphorylation level after 10 min of nitrogen resupply (late responses) included proteins involved in protein synthesis and degradation, as well as proteins with functions in central metabolism and hormone metabolism. Resupply of nitrogen in the form of nitrate or ammonium resulted in distinct phosphorylation patterns, mainly of proteins with signaling functions, transcription factors and transporters

"I need help": A study of spiritual distress among people diagnosed with bipolar disorder in Malaysia

Introduction: Little is known about spiritual distress in people with bipolar disorder, where they are inclined to maladaptive coping. Given the contextual influence of religion towards Malaysians, this study is aimed at exploring the phenomenon of spiritual despair and the recovery, as experienced by a group of people with bipolar disorder. Materials and method: A qualitative one-to-one interview was conducted on 25 participants of multi-religious background and diagnosed with bipolar disorder recruited from two psychiatric clinics in Kuala Lumpur, Malaysia. All interviews were audio-taped and transcribed verbatim. The interview data were analysed using a thematic analysis approach. Results: This paper presents the theme of ‘Restoring hope, meaning, and purpose’ with three subthemes; 1) experiencing spiritual despair, 2) engaging in spiritual meaning-making, and 3) orienting the spiritual life. The finding captured the participants’ experience of having despair in God over the ‘fated experience’ (takdir) and they abandoned religious practice during the period. However, participant narratives also indicate the possibility that their spiritual despair can be mitigated, endured, or transformed through the support of family members. The participants also highly recommended that spirituality may be combined with medical interventions, such as medication prescribed by their psychiatrist. Conclusion: This study highlights the importance of supportive family in advancing towards religious-spiritual pathways, and the advantages of medical interventions in terms of controlling the bipolar symptoms during their spiritual despair. This study is in favour of extending the role of mental health service in providing sessions for the users to voice out their spiritual concerns

The mental health benefits of religion and spirituality in people living with bipolar disorder in Malaysia

Introduction: The taxonomy of spirituality is fraught with complexities in relation to mental health studies, due to contextual variables such as religion. While positive mental health outcomes have been reported by many studies in relation to spirituality, little is known concerning spirituality’s effects in the context of bipolar disorder. This study aims to provide a contextual understanding of spirituality from the religious worldview of people with bipolar disorder. Method: This is a qualitative study involving semi-structured and one-to-one in-depth interviews. A total of 25 participants diagnosed with bipolar disorder were recruited from two psychiatric outpatient clinics. All interviews were audio-taped and transcribed verbatim by the researcher. Thematic analysis is used to analyze the data and Bourdieu’s concept of habitus is used to explore the findings in relation to participants’ subjective account on their religious form of spirituality. Finding: The theme ‘Maintaining a positive sense of self’ mainly consists of the religious element in the participants' everyday life: faith in God, religious practices and a sense of spiritual harmony (i.e. peacefulness and connectedness). The role of religion and social agency were inclusive in promoting spirituality among the study sample. Conclusion: Through Bourdieu’s lens of religious habitus, this study provides the understanding of religious-spirituality and a healthy mental state in such a way that it implies a relationship with God through religious beliefs and practices. This study invites other to pay attention to the dynamic roles of religious agency and society in promoting religious faith and practices among individuals diagnosed with bipolar disorder

“I Need Help”: A Study of Spiritual Distress Among People Diagnosed With Bipolar Disorder in Malaysia

Introduction: Little is known about spiritual distress in people with bipolar disorder, where they are inclined to maladaptive coping. Given the contextual influence of religion towards Malaysians, this study is aimed at exploring the phenomenon of spiritual despair and the recovery, as experienced by a group of people with bipolar disorder. Materials and method: A qualitative one-to-one interview was conducted on 25 participants of multi-religious background and diagnosed with bipolar disorder recruited from two psychiatric clinics in Kuala Lumpur, Malaysia. All interviews were audio-taped and transcribed verbatim. The interview data were analysed using a thematic analysis approach. Results: This paper presents the theme of ‘Restoring hope, meaning, and purpose’ with three subthemes; 1) experiencing spiritual despair, 2) engaging in spiritual meaning-making, and 3) orienting the spiritual life. The finding captured the participants’ experience of having despair in God over the ‘fated experience’ (takdir) and they abandoned religious practice during the period. However, participant narratives also indicate the possibility that their spiritual despair can be mitigated, endured, or transformed through the support of family members. The participants also highly recommended that spirituality may be combined with medical interventions, such as medication prescribed by their psychiatrist. Conclusion: This study highlights the importance of supportive family in advancing towards religious-spiritual pathways, and the advantages of medical interventions in terms of controlling the bipolar symptoms during their spiritual despair. This study is in favour of extending the role of mental health service in providing sessions for the users to voice out their spiritual concerns

Resonant nucleation of spatio-temporal order via parametric modal amplification

We investigate, analytically and numerically, the emergence of spatio-temporal order in nonequilibrium scalar field theories. The onset of order is triggered by destabilizing interactions (DIs), which instantaneously change the interacting potential from a single to a double-well, tunable to be either degenerate (SDW) or nondegenerate (ADW). For the SDW case, we observe the emergence of spatio-temporal coherent structures known as oscillons. We show that this emergence is initially synchronized, the result of parametric amplification of the relevant oscillon modes. We also discuss how these ordered structures act as bottlenecks for equipartition. For ADW potentials, we show how the same parametric amplification mechanism may trigger the rapid decay of a metastable state. For a range of temperatures, the decay rates associated with this resonant nucleation can be orders of magnitude larger than those computed by homogeneous nucleation, with time-scales given by a simple power law, $\tau_{\rm RN}\sim[E_b/k_BT]^B$, where $B$ depends weakly on the temperature and $E_b/k_BT$ is the free-energy barrier of a critical fluctuation.Comment: 38 pages, 20 figures now included within the tex

The distorted body: the perception of the relative proportions of the body is preserved in Parkinson’s disease

Given humans’ ubiquitous visual experience of their own body, one reasonable assumption is that one’s perceptions of the lengths of their body parts should be accurate. However, recent research has shown that large systematic distortions of the length of body parts are present in healthy younger adults. These distortions appear to be linked to tactile sensitivity such that individuals overestimate the length of body parts of low tactile sensitivity to a greater extent than body parts of high tactile sensitivity. There are certain conditions featuring reduced tactile sensitivity, such as Parkinson’s Disease (PD) and healthy older ageing. However, the effect of these circumstances on individuals’ perceptions of the lengths of their body parts remains unknown. In this study, participants visually estimated the length of their body parts using their hand as a metric. We show that despite the reductions in tactile sensitivity, and potential alterations in the cortical presentation of body parts that may occur in PD and healthy older ageing, individuals with mild-moderate PD and older adults of comparable age experience body size distortions comparable to healthy younger controls. These findings demonstrate that the ability to perceive the length of one’s body parts is well preserved in mild-moderate PD

A database of noble gases in lunar samples in preparation for mass spectrometry on the Moon

The lunar regolith provides a temporal archive of the evolution of the Moon and inner Solar System over the last ~4 billion years. During this time, noble gases have been trapped and produced within soils and rocks at the lunar surface. These noble gas concentrations can be used to unravel the history of lunar material and shed light on processes that have evolved the surface of the Moon through time. We have collected published noble gas data for a range of lunar samples including soils, regolith breccias, crystalline (e.g., mare basalts, anorthosite) and impact-melt rocks. The compilation includes noble gas concentrations and isotope ratios for He, Ne, Ar, Kr and Xe; trapped, cosmogenic and radiogenic isotopes; and cosmic ray exposure ages. We summarise the significance of these data, which can be used as a baseline for expected noble gas concentrations in a range of lunar samples, and provide a framework for future in situ noble gas measurements on the lunar surface

The Isotope Effect in d-Wave Superconductors

Based on recently proposed anti-ferromagnetic spin fluctuation exchange models for $d_{x^2-y^2}$-superconductors, we show that coupling to harmonic phonons {\it{cannot}} account for the observed isotope effect in the cuprate high-$T_c$ materials, whereas coupling to strongly anharmonic multiple-well lattice tunneling modes {\it{can}}. Our results thus point towards a strongly enhanced {\it{effective}} electron-phonon coupling and a possible break-down of Migdal-Eliashberg theory in the cuprates.Comment: 12 pages + 2 figures, Postscript files, all uuencoded Phys. Rev. Lett. (1995, to be published

Reinforcement of poly-l-lactic acid electrospun membranes with strontium borosilicate bioactive glasses for bone tissue engineering

Herein, for the first time, we combined poly-l-lactic acid (PLLA) with a strontium borosilicate bioactive glass (BBG-Sr) using electrospinning to fabricate a composite bioactive PLLA membrane loaded with 10% (w/w) of BBG-Sr glass particles (PLLA-BBG-Sr). The composites were characterised by scanning electron microscopy (SEM) and microcomputer tomography (μ-CT), and the results showed that we successfully fabricated smooth and uniform fibres (1-3μm in width) with a homogeneous distribution of BBG-Sr microparticles (<45μm). Degradation studies (in phosphate buffered saline) demonstrated that the incorporation of BBG-Sr glass particles into the PLLA membranes increased their degradability and water uptake with a continuous release of cations. The addition of BBG-Sr glass particles enhanced the membrane's mechanical properties (69% higher Young modulus and 36% higher tensile strength). Furthermore, cellular in vitro evaluation using bone marrow-derived mesenchymal stem cells (BM-MSCs) demonstrated that PLLA-BBG-Sr membranes promoted the osteogenic differentiation of the cells as demonstrated by increased alkaline phosphatase activity and up-regulated osteogenic gene expression (Alpl, Sp7 and Bglap) in relation to PLLA alone. These results strongly suggest that the composite PLLA membranes reinforced with the BBG-Sr glass particles have potential as an effective biomaterial capable of promoting bone regeneration. STATEMENT OF SIGNIFICANCE: PLLA membranes were reinforced with 10% (w/w) of strontium-bioactive borosilicate glass microparticles, and their capacity to induce the osteogenic differentiation of bone marrow mesenchymal stem cells (BM-MSCs) was evaluated. These membranes presented an increased: degradability, water uptake, Young modulus and tensile strength. We also demonstrated that these membranes are non-cytotoxic and promote the attachment of BM-MSCs. The addition of the glass microparticles into the PLLA membranes promoted the increase of ALP activity (under osteogenic conditions), as well as the BM-MSCs osteogenic differentiation as shown by the upregulation of Alpl, Sp7 and Bglap gene expression. Overall, we demonstrated that the reinforcement of PLLA with glass microparticles results in a biomaterial with the appropriate properties for the regeneration of bone tissue

Unveiling Soft Gamma-Ray Repeaters with INTEGRAL

Thanks to INTEGRAL's long exposures of the Galactic Plane, the two brightest Soft Gamma-Ray Repeaters, SGR 1806-20 and SGR 1900+14, have been monitored and studied in detail for the first time at hard-X/soft gamma rays. This has produced a wealth of new scientific results, which we will review here. Since SGR 1806-20 was particularly active during the last two years, more than 300 short bursts have been observed with INTEGRAL. and their characteristics have been studied with unprecedented sensitivity in the 15-200 keV range. A hardness-intensity anticorrelation within the bursts has been discovered and the overall Number-Intensity distribution of the bursts has been determined. In addition, a particularly active state, during which ~100 bursts were emitted in ~10 minutes, has been observed on October 5 2004, indicating that the source activity was rapidly increasing. This eventually led to the Giant Flare of December 27th 2004, for which a possible soft gamma-ray (>80 keV) early afterglow has been detected. The deep observations allowed us to discover the persistent emission in hard X-rays (20-150 keV) from 1806-20 and 1900+14, the latter being in a quiescent state, and to directly compare the spectral characteristics of all Magnetars (two SGRs and three Anomalous X-ray Pulsars) detected with INTEGRAL.Comment: 8 pages, 7 figures, Presented at the conference "Isolated Neutron Stars: from the Surface to the Interior", London, UK, 24-28 April 200