Moving Beyond "Risky Sex": Adolescent Sexual Resilience and Sexual Health in Young Adulthood

Indiana University-Purdue University Indianapolis (IUPUI)Sexual behaviors in adolescence establish the initial resources an individual carries into sexual relationships in adulthood. Current definitions of sexual resilience in adolescence are defined from a negative, risk-based lens. Resilience theory, more generally defined, considers both internal and external factors that promote adaptation to challenging situations. A direct, capital-based approach to studying adolescent sexual resilience has not been found in the extant literature and I propose that a new, more inclusive definition of sexual resilience in adolescence will be more strongly correlated with sexual health in young adults than the risk-based definition. This study creates mutually exclusive risk-based and capital-based measures of adolescent sexual resilience and examines their associations with sexual health outcomes in young adulthood. The data did not produce significant findings, yet descriptive results provide direction for future research. Research in this area is of critical importance as adolescence and young adulthood are unique life stages that involve significant development in areas that influence health, both short and long term. This research, through a proper resilience lens, will better guide adolescent sexual education to develop internal resources as well as provide adequate external resources for adolescents that promote better sexual health and agency outcomes in adulthood

Social Norms: Do We Love Norms Too Much?

Social norms are often cited as the cause of many social phenomena, especially as an explanation for prosocial family and relationship behaviors. And yet maybe we love the idea of social norms too much, as suggested by our failure to subject them to rigorous test. Compared to the detail in social norms theoretical orientations, there is very little detail in tests of normative theories. To provide guidance to researchers who invoke social norms as explanations, we catalog normative orientations that have been proposed to account for consistent patterns of action. We call on researchers to conduct tests of normative theories and the processes such theories assert

The role of the CTD phosphatase Rrt1 and post-translational modifications in regulation of RNA polymerase II

Indiana University-Purdue University Indianapolis (IUPUI)RNA polymerase II (RNAPII) is regulated by multiple modifications to the C-terminal domain (CTD) of the largest subunit, Rpb1. This study has focused on the relationship between hyperphosphorylation of the CTD and RNAPII turnover and proteolytic degradation as well as post-translational modifications of the globular core of RNAPII. Following tandem affinity purification, western blot analysis showed that MG132 treated RTR1 ERG6 deletion yeast cells have accumulation of total RNAPII and in particular, the hyperphosphorylated form of the protein complex. In addition, proteomic studies using MuDPIT have revealed increased interaction between proteins of the ubiquitin-proteasome degradation system in the mutant MG132 treated yeast cells as well as potential ubiquitin and phosphorylation sites in RNAPII subunits, Rpb6 and Rpb1, respectively. A novel Rpb1 phosphorylation site, T1471-P, is located in the linker region between the CTD and globular domain of Rpb1 and will be the focus of future studies to determine biological significance of this post-translational modification

Trichoderma reesei Contains a Biosynthetic Gene Cluster That Encodes the Antifungal Agent Ilicicolin H

The trili biosynthetic gene cluster (BGC) from the well-studied organism Trichoderma reesei was studied by heterologous expression in the fungal host Aspergillus oryzae. Coexpression of triliA and triliB produces two new acyl tetramic acids. Addition of the ring-expanding cytochrome P450 encoded by triliC then yields a known pyridone intermediate to ilicicolin H and a new chain-truncated shunt metabolite. Finally, addition of the intramolecular Diels-Alderase encoded by triliD affords a mixture of 8-epi ilicicolin H and ilicicolin H itself, showing that the T. reesei trili BGC encodes biosynthesis of this potent antifungal agent. Unexpected A. oryzae shunt pathways are responsible for the production of the new compounds, emphasising the role of fungal hosts in catalysing diversification reactions

Heterologous Expression of Secondary Metabolite Genes in Trichoderma reesei for Waste Valorization

Trichoderma reesei (Hypocrea jecorina) was developed as a microbial cell factory for the heterologous expression of fungal secondary metabolites. This was achieved by inactivation of sorbicillinoid biosynthesis and construction of vectors for the rapid cloning and expression of heterologous fungal biosynthetic genes. Two types of megasynth(et)ases were used to test the strain and vectors, namely a non-reducing polyketide synthase (nr-PKS, aspks1) from Acremonium strictum and a hybrid highly-reducing PKS non-ribosomal peptide synthetase (hr-PKS-NRPS, tenS + tenC) from Beauveria bassiana. The resulting engineered T. reesei strains were able to produce the expected natural products 3-methylorcinaldehyde and pretenellin A on waste materials including potato, orange, banana and kiwi peels and barley straw. Developing T. reesei as a heterologous host for secondary metabolite production represents a new method for waste valorization by the direct conversion of waste biomass into secondary metabolites

Late Summer Planted Oat-Brassica Forage Quality Changes during Winter Grazing

Oat, radish, and turnip samples were taken on pivots being grazed from November through January in Clay Center, Nebraska. The objective was to determine how the quality changed throughout the winter. The forage was observed to be high-quality (highly digestibility with moderate CP content). Digestibility did decline over this period but minimal changes in CP content were observed. From early November to early January, the digestibility of oats appeared to decline more (10% unit decline) than turnip and radish leaves (5% unit decrease). However, digestibility (67% IVOMD) and CP content (15%) of oats in early January were still as high as a good quality grass hay. Brassica (radish and turnip) leaves were more similar to a concentrate (81–83% IVOMD and 23–26% CP) even in January. Thus, even though the forage changed color from green to brown after hard freezes, the forage still had good feed value in January

The Nainital-Cape Survey -- II:Report for pulsation in five chemically peculiar A-type stars and presentation of 140 null results

To search photometric variability in chemically peculiar A type stars in the northern hemisphere. High-speed photometric observations of Ap and Am star candidates have been carried out from ARIES (Manora Peak, Nainital) using a three-channel fast photometer attached to the ARIES 104-cm Sampurnanand telescope. This paper presents three new variables: HD 113878, HD 118660 and HD 207561. During the time span of the survey (1999 December to 2004 January) pulsations of the $\delta$ Sct type were also found for the two evolved Am stars HD 102480 and HD 98851, as reported in Joshi et al. (2002, 2003). Additionally, we present 140 null results of the survey for this time span. The star HD 113878 pulsates with a period of 2.31 hr, which is typical of $\delta$ Sct stars. HD 118660 exhibits multi-periodic variability with a prominent period of nearly 1 hr. These periods need to be investigated and make HD 118660 a particularly interesting target for further observations. For HD 207561, a star classified as Am, a probable pulsation with a period of 6 min was found in the light curves obtained on two consecutive nights. Both HD 102480 and HD 98851 exhibit unusual alternating high and low amplitude maxima, with a period ratio of 2:1. The analysis of the null results confirms the photometric quality of the NainitalComment: 14 pages, 13 figures, Accepted for publication in A&

Development and Preliminary Evaluation of a Telephone-based Mindfulness Training Intervention for Survivors of Critical Illness

Rationale: Persistent symptoms of psychological distress represent an unmet need among intensive care unit (ICU) survivors. Objectives: We aimed to develop and pilot test a simple telephone-based mindfulness training intervention to address this population’s unique needs. Methods: Open trial involving survivors of medical and surgical critical illness and their informal caregivers, using a pretest–posttest design. Measurements and Main Results: We developed a six-session, telephone-delivered, ICU survivor–specific mindfulness intervention based on past focus groups, the medical literature, and the precedent of the most effective components of existing mindfulness programs. A total of 11 survivors of mechanical ventilation were enrolled, together with 2 informal caregivers for exploratory purposes. Three patients dropped out before intervention initiation because of progressive illness or severe social stressors. Of the 10 remaining participants, 8 (80%) completed the program within 7 weeks. Among these eight patients and caregivers who completed all study procedures, six (75%) experienced improvement in symptoms of psychological distress (anxiety, depression, or post-traumatic stress disorder). Changes in distress symptoms were correlated with improvement in mindfulness qualities, adaptive coping, and emotion regulation. Participants reported high satisfaction with the program in postintervention interviews. Conclusions: A new ICU survivor–specific mindfulness training intervention delivered by telephone was acceptable and feasible. Changes in symptoms of distress were correlated with changes in skills that were targeted by the mindfulness program. Controlled trials are needed to further evaluate this promising intervention