Antarctic Circumpolar Transport and the Southern Mode: a model investigation of interannual to decadal time scales

Abstract. It is well-established that, at periods shorter than a year, variations in Antarctic Circumpolar Transport are reflected in a barotropic mode, known as the Southern Mode, in which sea level and bottom pressure varies coherently around Antarctica. Here, we use two multidecadal ocean model runs to investigate the behaviour of the Southern Mode at time scales on which density changes become important, leading to a baroclinic component to the adjustment. We find that the concept of a Southern Mode in bottom pressure remains valid, and remains a direct measure of the circumpolar transport, with changes at the northern boundary playing only a small role even on decadal time scales. However, at periods longer than about 5 yr, density changes start to play a role, leading to a surface intensification of the vertical profile of the transport. We also find that barotropic currents on the continental slope account for a significant fraction of the variability, and produce surface intensification in the meridional-integral flow. The role of density variations results in a sea level signal which, although reflecting transport changes at all time scales, has a ratio of sea level to transport which becomes larger at longer time scales. This means that any long-term transport monitoring strategy based on present measurement systems must involve multiplying the observed quantity by a factor which depends on frequency. </jats:p

Future Evolution of an Eddy Rich Ocean Associated with Enhanced East Atlantic Storminess in a Coupled Model Projection

This is the final version. Available on open access from the American Geophysical Union via the DOI in this recordData Availability Statement: The ERA5 reanalysis data set is available from https://www.ecmwf.int/en/forecasts/datasets/reanalysis-datasets/era5. Some of the model runs used the ARCHER UK National Supercomputing Service (http://www.archer.ac.uk).Improved representation of air-sea fluxes afforded by eddy-rich oceans in high-resolution coupled ocean-atmosphere models may modify the tracks and intensity of storms and their response to climate change. We examine changes in winter surface ocean conditions and storminess associated with moving from an eddy-permitting (1/4°, HM) to an eddy-rich (1/12°, HH) ocean in control and climate change (SSP585) simulations of the HadGEM3-GC3.1 model in which atmosphere resolution is kept at 25 km. Differences in North Atlantic climate in the control runs stem from a revised location of the Gulf Stream in the eddy-rich model. Projections reveal greater warming in the western Atlantic in HH than HM and a pronounced increase in eastern Atlantic storminess with changes six times greater than in the eddy-permitting model. This increase is associated with the distinctive long-term evolution of the North Atlantic warming hole and the Gulf Stream separation in the eddy-rich model.European Union Horizon 2020Natural Environment Research Council (NERC)Met Office Hadley Centre Climate Programm

SST dynamics at different scales: evaluating the oceanographic model resolution skill to represent SST processes in the Southern Ocean

In this study we demonstrate the many strengths of scale analysis: we use it to evaluate the Nucleus for European Modelling of the Ocean (NEMO) model skill in representing sea surface temperature (SST) in the Southern Ocean (SO) by comparing three model resolutions: 1/12°, 1/4° and 1°. We show that whilst 4‐5 times resolution scale is sufficient for each model resolution to reproduce the magnitude of satellite Earth Observation (EO) SST spatial variability to within ±10%, the representation of ∼ 100 km SST variability patterns is substantially (e.g ∼50% at 750 km) improved by increasing model resolution from 1° to 1/12°. We also analysed the dominant scales of the SST model input drivers (short‐wave radiation, air‐sea heat fluxes, wind stress components, wind stress curl, bathymetry) variability with the purpose of determining the optimal SST model input driver resolution. The SST magnitude of variability is shown to scale with two power law regimes separated by a scaling break at ∼200 km scale. The analysis of the spatial and temporal scales of dominant SST driver impact helps to interpret this scaling break as a separation between two different dynamical regimes: the (relatively) fast SST dynamics below ∼200 km governed by eddies, fronts, Ekman upwelling and air‐sea heat exchange, whilst above ∼200 km the SST variability is dominated by long‐term (seasonal and supra‐seasonal) modes and the SST geography

Understanding long-term human ecodynamics through the lens of ecosystem collapse

Most research on long-term human ecodynamics examines changes in the size and structure of human populations, often in relation to climate change. Here we offer an alternative perspective that draws on recent progress in conservation science, examining the causes and consequences of ecosystem collapse. We identify human actions that can cause abrupt transformation of ecosystems, in relation to key mechanisms and underlying theory. Such ecosystem collapse can in turn affect human societies by altering flows of ecosystem benefits to people. In this way, human ecodynamics can be understood by separately analysing the dynamics of social and ecological sub-systems, which are reciprocally linked. Ecosystem collapse represents a perturbation of these sub-systems, and provides insights into the mechanisms underlying their respective dynamics. We illustrate this approach through four case studies, which examine the spread of agriculture during the Holocene. Four key knowledge gaps emerge through consideration of these case studies: the linkages between social and ecological sub-systems, and how these change over time; the presence of feedbacks between these sub-systems; the relationships between local- and regional-scale collapse; and the relationships with ecological recovery. Increased research on ecosystem collapse could help clarify the relative influence of environmental degradation on societal dynamics, while providing insights into resilience and sustainability. Given the outstanding societal importance of ecosystem collapse, such research could also strengthen the relevance of historical sciences to the contemporary world

An information theoretic approach to insulin sensing by human kidney podocytes

This is the author accepted manuscript. The final version is available from the publisher via the DOI in this recordPodocytes are key components of the glomerular filtration barrier (GFB). They are insulin-responsive but can become insulin-resistant, causing features of the leading global cause of kidney failure, diabetic nephropathy. Insulin acts via insulin receptors to control activities fundamental to GFB integrity, but the amount of information transferred is unknown. Here we measure this in human podocytes, using information theory-derived statistics that take into account cell-cell variability. High content imaging was used to measure insulin effects on Akt, FOXO and ERK. Mutual Information (MI) and Channel Capacity (CC) were calculated as measures of information transfer. We find that insulin acts via noisy communication channels with more information flow to Akt than to ERK. Information flow estimates were increased by consideration of joint sensing (ERK and Akt) and response trajectory (live cell imaging of FOXO1-clover translocation). Nevertheless, MI values were always <1Bit as most information was lost through signaling. Constitutive PI3K activity is a predominant feature of the system that restricts the proportion of CC engaged by insulin. Negative feedback from Akt supressed this activity and thereby improved insulin sensing, whereas sensing was robust to manipulation of feedforward signaling by inhibiting PI3K, PTEN or PTP1B. The decisions made by individual podocytes dictate GFB integrity, so we suggest that understanding the information on which the decisions are based will improve understanding of diabetic kidney disease and its treatment.Kidney Research UK Gran

The effects of vaccination and immunity on bacterial infection dynamics in vivo.

Salmonella enterica infections are a significant global health issue, and development of vaccines against these bacteria requires an improved understanding of how vaccination affects the growth and spread of the bacteria within the host. We have combined in vivo tracking of molecularly tagged bacterial subpopulations with mathematical modelling to gain a novel insight into how different classes of vaccines and branches of the immune response protect against secondary Salmonella enterica infections of the mouse. We have found that a live Salmonella vaccine significantly reduced bacteraemia during a secondary challenge and restrained inter-organ spread of the bacteria in the systemic organs. Further, fitting mechanistic models to the data indicated that live vaccine immunisation enhanced both the bacterial killing in the very early stages of the infection and bacteriostatic control over the first day post-challenge. T-cell immunity induced by this vaccine is not necessary for the enhanced bacteriostasis but is required for subsequent bactericidal clearance of Salmonella in the blood and tissues. Conversely, a non-living vaccine while able to enhance initial blood clearance and killing of virulent secondary challenge bacteria, was unable to alter the subsequent bacterial growth rate in the systemic organs, did not prevent the resurgence of extensive bacteraemia and failed to control the spread of the bacteria in the body.This work was supported by the Biotechnology and Biological Sciences Research Council [grant number BB/I002189/1].This is the published manuscript. It was originally published by PLOS One here: http://www.plospathogens.org/article/info%3Adoi%2F10.1371%2Fjournal.ppat.1004359

Measurement of D-s(+) and D-s(*+) production in B meson decays and from continuum e(+)e(-) annihilation at √s=10.6 GeV

This is the pre-print version of the Article. The official published version can be accessed from the links below. Copyright @ 2002 APSNew measurements of Ds+ and Ds*+ meson production rates from B decays and from qq̅ continuum events near the Υ(4S) resonance are presented. Using 20.8 fb-1 of data on the Υ(4S) resonance and 2.6 fb-1 off-resonance, we find the inclusive branching fractions B(B⃗Ds+X)=(10.93±0.19±0.58±2.73)% and B(B⃗Ds*+X)=(7.9±0.8±0.7±2.0)%, where the first error is statistical, the second is systematic, and the third is due to the Ds+→φπ+ branching fraction uncertainty. The production cross sections σ(e+e-→Ds+X)×B(Ds+→φπ+)=7.55±0.20±0.34pb and σ(e+e-→Ds*±X)×B(Ds+→φπ+)=5.8±0.7±0.5pb are measured at center-of-mass energies about 40 MeV below the Υ(4S) mass. The branching fractions ΣB(B⃗Ds(*)+D(*))=(5.07±0.14±0.30±1.27)% and ΣB(B⃗Ds*+D(*))=(4.1±0.2±0.4±1.0)% are determined from the Ds(*)+ momentum spectra. The mass difference m(Ds+)-m(D+)=98.4±0.1±0.3MeV/c2 is also measured.This work was supported by DOE and NSF (USA), NSERC (Canada), IHEP (China), CEA and CNRS-IN2P3 (France), BMBF (Germany), INFN (Italy), NFR (Norway), MIST (Russia), and PPARC (United Kingdom). Individuals have received support from the Swiss NSF, A. P. Sloan Foundation, Research Corporation, and Alexander von Humboldt Foundation

Comparative physical maps derived from BAC end sequences of tilapia (Oreochromis niloticus)

Background: The Nile tilapia is the second most important fish in aquaculture. It is an excellent laboratory model, and is closely related to the African lake cichlids famous for their rapid rates of speciation. A suite of genomic resources has been developed for this species, including genetic maps and ESTs. Here we analyze BAC endsequences to develop comparative physical maps, and estimate the number of genome rearrangements, between tilapia and other model fish species. Results: We obtained sequence from one or both ends of 106,259 tilapia BACs. BLAST analysis against the genome assemblies of stickleback, medaka and pufferfish allowed identification of homologies for approximately 25,000 BACs for each species. We calculate that rearrangement breakpoints between tilapia and these species occur about every 3 Mb across the genome. Analysis of 35,000 clones previously assembled into contigs by restriction fingerprints allowed identification of longer-range syntenies. Conclusions: Our data suggest that chromosomal evolution in recent teleosts is dominated by alternate loss of gene duplicates, and by intra-chromosomal rearrangements (~one per million years). These physical maps are a useful resource for comparative positional cloning of traits in cichlid fishes. The paired BAC end sequences from these clones will be an important resource for scaffolding forthcoming shotgun sequence assemblies of the tilapia genome. (Résumé d'auteur

The Testicular and Epididymal Expression Profile of PLCζ in Mouse and Human Does Not Support Its Role as a Sperm-Borne Oocyte Activating Factor

Phospholipase C zeta (PLCζ) is a candidate sperm-borne oocyte activating factor (SOAF) which has recently received attention as a potential biomarker of human male infertility. However, important SOAF attributes of PLCζ, including its developmental expression in mammalian spermiogenesis, its compartmentalization in sperm head perinuclear theca (PT) and its release into the ooplasm during fertilization have not been established and are addressed in this investigation. Different detergent extractions of sperm and head/tail fractions were compared for the presence of PLCζ by immunoblotting. In both human and mouse, the active isoform of PLCζ was detected in sperm fractions other than PT, where SOAF is expected to reside. Developmentally, PLCζ was incorporated as part of the acrosome during the Golgi phase of human and mouse spermiogenesis while diminishing gradually in the acrosome of elongated spermatids. Immunofluorescence localized PLCζ over the surface of the postacrosomal region of mouse and bull and head region of human spermatozoa leading us to examine its secretion in the epididymis. While previously thought to have strictly a testicular expression, PLCζ was found to be expressed and secreted by the epididymal epithelial cells explaining its presence on the sperm head surface. In vitro fertilization (IVF) revealed that PLCζ is no longer detectable after the acrosome reaction occurs on the surface of the zona pellucida and thus is not incorporated into the oocyte cytoplasm for activation. In summary, we show for the first time that PLCζ is compartmentalized as part of the acrosome early in human and mouse spermiogenesis and is secreted during sperm maturation in the epididymis. Most importantly, no evidence was found that PLCζ is incorporated into the detergent-resistant perinuclear theca fraction where SOAF resides