3 research outputs found
Combining mobile-health (mHealth) and artificial intelligence (AI) methods to avoid suicide attempts: the Smartcrises study protocol
The screening of digital footprint for clinical purposes relies on the capacity of wearable technologies
to collect data and extract relevant informationâs for patient management. Artificial intelligence (AI) techniques
allow processing of real-time observational information and continuously learning from data to build
understanding. We designed a system able to get clinical sense from digital footprints based on the smartphoneâs
native sensors and advanced machine learning and signal processing techniques in order to identify suicide risk.
Method/design: The Smartcrisis study is a cross-national comparative study. The study goal is to determine the
relationship between suicide risk and changes in sleep quality and disturbed appetite. Outpatients from the
Hospital FundaciĂłn JimĂ©nez DĂaz Psychiatry Department (Madrid, Spain) and the University Hospital of Nimes
(France) will be proposed to participate to the study. Two smartphone applications and a wearable armband will
be used to capture the data. In the intervention group, a smartphone application (MEmind) will allow for the
ecological momentary assessment (EMA) data capture related with sleep, appetite and suicide ideations.
Discussion: Some concerns regarding data security might be raised. Our system complies with the highest level of
security regarding patientsâ data. Several important ethical considerations related to EMA method must also be
considered. EMA methods entails a non-negligible time commitment on behalf of the participants. EMA rely on
daily, or sometimes more frequent, Smartphone notifications. Furthermore, recording participantsâ daily experiences
in a continuous manner is an integral part of EMA. This approach may be significantly more than asking a
participant to complete a retrospective questionnaire but also more accurate in terms of symptoms monitoring.
Overall, we believe that Smartcrises could participate to a paradigm shift from the traditional identification of risks
factors to personalized prevention strategies tailored to characteristics for each patientThis study was partly funded by FundaciĂłn JimĂ©nez DĂaz Hospital, Instituto
de Salud Carlos III (PI16/01852), DelegaciĂłn del Gobierno para el Plan
Nacional de Drogas (20151073), American Foundation for Suicide Prevention
(AFSP) (LSRG-1-005-16), the Madrid Regional Government (B2017/BMD-3740
AGES-CM 2CM; Y2018/TCS-4705 PRACTICO-CM) and Structural Funds of the
European Union. MINECO/FEDER (âADVENTUREâ, id. TEC2015â69868-C2â1-R)
and MCIU Explora Grant âaMBITIONâ (id. TEC2017â92552-EXP), the French Embassy
in Madrid, Spain, The foundation de lâavenir, and the Fondation de
France. The work of D. RamĂrez and A. ArtĂ©s-RodrĂguez has been partly supported
by Ministerio de EconomĂa of Spain under projects: OTOSIS
(TEC2013â41718-R), AID (TEC2014â62194-EXP) and the COMONSENS Network
(TEC2015â69648-REDC), by the Ministerio de EconomĂa of Spain jointly with
the European Commission (ERDF) under projects ADVENTURE (TEC2015â
69868-C2â1-R) and CAIMAN (TEC2017â86921-C2â2-R), and by the Comunidad
de Madrid under project CASI-CAM-CM (S2013/ICE-2845). The work of P.
Moreno-Muñoz has been supported by FPI grant BES-2016-07762
Genetic depletion and pharmacological targeting of αv integrin in breast cancer cells impairs metastasis in zebrafish and mouse xenograft models
Co-receptor Binding Site Antibodies Enable CD4-Mimetics to Expose Conserved Anti-cluster A ADCC Epitopes on HIV-1 Envelope Glycoproteins
Human immunodeficiency virus type 1 (HIV-1) has evolved a sophisticated strategy to conceal conserved epitopes of its envelope glycoproteins (Env) recognized by antibody-dependent cellular cytotoxicity (ADCC)-mediating antibodies. These antibodies, which are present in the sera of most HIV-1-infected individuals, preferentially recognize Env in its CD4-bound conformation. Accordingly, recent studies showed that small CD4-mimetics (CD4mc) able to âpushâ Env into this conformation sensitize HIV-1-infected cells to ADCC mediated by HIV+ sera. Here we test whether CD4mc also expose epitopes recognized by anti-cluster A monoclonal antibodies such as A32, thought to be responsible for the majority of ADCC activity present in HIV+ sera and linked to decreased HIV-1 transmission in the RV144 trial. We made the surprising observation that CD4mc are unable to enhance recognition of HIV-1-infected cells by this family of antibodies in the absence of antibodies such as 17b, which binds a highly conserved CD4-induced epitope overlapping the co-receptor binding site (CoRBS). Our results indicate that CD4mc initially open the trimeric Env enough to allow the binding of CoRBS antibodies but not anti-cluster A antibodies. CoRBS antibody binding further opens the trimeric Env, allowing anti-cluster A antibody interaction and sensitization of infected cells to ADCC. Therefore, ADCC responses mediated by cluster A antibodies in HIV-positive sera involve a sequential opening of the Env trimer on the surface of HIV-1-infected cells. The understanding of the conformational changes required to expose these vulnerable Env epitopes might be important in the design of new strategies aimed at fighting HIV-1