164 research outputs found
Tumbleweeds and airborne gravitational noise sources for LIGO
Gravitational-wave detectors are sensitive not only to astrophysical
gravitational waves, but also to the fluctuating Newtonian gravitational forces
of moving masses in the ground and air around the detector. This paper studies
the gravitational effects of density perturbations in the atmosphere, and from
massive airborne objects near the detector. These effects were previously
considered by Saulson; in this paper I revisit these phenomena, considering
transient atmospheric shocks, and the effects of sound waves or objects
colliding with the ground or buildings around the test masses. I also consider
temperature perturbations advected past the detector as a source of
gravitational noise. I find that the gravitational noise background is below
the expected noise floor even of advanced interferometric detectors, although
only by an order of magnitude for temperature perturbations carried along
turbulent streamlines. I also find that transient shockwaves in the atmosphere
could potentially produce large spurious signals, with signal-to-noise ratios
in the hundreds in an advanced interferometric detector. These signals could be
vetoed by means of acoustic sensors outside of the buildings. Massive
wind-borne objects such as tumbleweeds could also produce gravitational signals
with signal-to-noise ratios in the hundreds if they collide with the
interferometer buildings, so it may be necessary to build fences preventing
such objects from approaching within about 30m of the test masses.Comment: 15 pages, 10 PostScript figures, uses REVTeX4.cls and epsfig.st
Tropospheric Phase Calibration in Millimeter Interferometry
We review millimeter interferometric phase variations caused by variations in
the precipitable water vapor content of the troposphere, and we discuss
techniques proposed to correct for these variations. We present observations
with the Very Large Array at 22 GHz and 43 GHz designed to test these
techniques. We find that both the Fast Switching and Paired Array calibration
techniques are effective at reducing tropospheric phase noise for radio
interferometers. In both cases, the residual rms phase fluctuations after
correction are independent of baseline length for b > b_{eff}. These techniques
allow for diffraction limited imaging of faint sources on arbitrarily long
baselines at mm wavelengths. We consider the technique of tropospheric phase
correction using a measurement of the precipitable water vapor content of the
troposphere via a radiometric measurement of the brightness temperature of the
atmosphere. Required sensitivities range from 20 mK at 90 GHz to 1 K at 185 GHz
for the MMA, and 120 mK for the VLA at 22 GHz. The minimum gain stability
requirement is 200 at 185 GHz at the MMA assuming that the astronomical
receivers are used for radiometry. This increases to 2000 for an uncooled
system. The stability requirement is 450 for the cooled system at the VLA at 22
GHz. To perform absolute radiometric phase corrections also requires knowledge
of the tropospheric parameters and models to an accuracy of a few percent. It
may be possible to perform an `empirically calibrated' radiometric phase
correction, in which the relationship between fluctuations in brightness
temperature differences with fluctuations in interferometric phases is
calibrated by observing a celestial calibrator at regular intervals.Comment: AAS LATEX preprint format. to appear in Radio Science 199
A New Strategy for Deep Wide-Field High Resolution Optical Imaging
We propose a new strategy for obtaining enhanced resolution (FWHM = 0.12
arcsec) deep optical images over a wide field of view. As is well known, this
type of image quality can be obtained in principle simply by fast guiding on a
small (D = 1.5m) telescope at a good site, but only for target objects which
lie within a limited angular distance of a suitably bright guide star. For high
altitude turbulence this 'isokinetic angle' is approximately 1 arcminute. With
a 1 degree field say one would need to track and correct the motions of
thousands of isokinetic patches, yet there are typically too few sufficiently
bright guide stars to provide the necessary guiding information. Our proposed
solution to these problems has two novel features. The first is to use
orthogonal transfer charge-coupled device (OTCCD) technology to effectively
implement a wide field 'rubber focal plane' detector composed of an array of
cells which can be guided independently. The second is to combine measured
motions of a set of guide stars made with an array of telescopes to provide the
extra information needed to fully determine the deflection field. We discuss
the performance, feasibility and design constraints on a system which would
provide the collecting area equivalent to a single 9m telescope, a 1 degree
square field and 0.12 arcsec FWHM image quality.Comment: 46 pages, 22 figures, submitted to PASP, a version with higher
resolution images and other supplementary material can be found at
http://www.ifa.hawaii.edu/~kaiser/wfhr
The Impact of Atmospheric Fluctuations on Degree-scale Imaging of the Cosmic Microwave Background
Fluctuations in the brightness of the Earth's atmosphere originating from
water vapor are an important source of noise for ground-based instruments
attempting to measure anisotropy in the Cosmic Microwave Background. This paper
presents a model for the atmospheric fluctuations and derives simple
expressions to predict the contribution of the atmosphere to experimental
measurements. Data from the South Pole and from the Atacama Desert in Chile,
two of the driest places on Earth, are used to assess the level of fluctuations
at each site.Comment: 29 pages, 7 figures, 1 table, appears in The Astrophysical Journa
Formulation of hydrophobic peptides for skin delivery via coated microneedles
Microneedles (MNs) have been investigated as a minimally-invasive delivery technology for a range of active pharmaceutical ingredients (APIs). Various formulations and methods for coating the surface of MNs with therapeutics have been proposed and exemplified, predominantly for hydrophilic drugs and particulates. The development of effective MN delivery formulations for hydrophobic drugs is more challenging with dosing restrictions and the use of organic solvents impacting on both the bioactivity and the kinetics of drug release. In this study we propose a novel formulation that is suitable for MN coating of hydrophobic auto-antigen peptides currently being investigated for antigen specific immunotherapy (ASI) of type 1 diabetes. The formulation, comprising three co-solvents (water, 2-methyl-2-butanol and acetic acid) and polyvinylalcohol 2000 (PVA2000) can dissolve both hydrophilic and hydrophobic peptide auto-antigens at relatively high, and clinically relevant, concentrations (25 mg/ml or 12.5 mg/ml). The drug:excipient ratio is restricted to 10:1 w/w to maximise dose whilst ensuring that the dry-coated payload does not significantly impact on MN skin penetration performance. The coating formulation and process does not adversely affect the biological activity of the peptide. The delivery efficiency of the coated peptide into skin is influenced by a number of parameters. Electropolishing the metal MN surface increases delivery efficiency from 2.0 ± 1.0% to 59.9 ± 6.7%. An increased mass of peptide formulation per needle, from 0.37 μg to 2 μg peptide dose, resulted in a thicker coating and a 20% reduction in the efficiency of skin delivery. Other important performance parameters for coated MNs include the role of excipients in assisting dissolution from the MNs, the intrinsic hydrophobicity of the peptide and the species of skin model used in laboratory studies. This study therefore both exemplifies the potential of a novel formulation for coating hydrophobic and hydrophilic peptides onto MN devices and provides new insight into the factors that influence delivery efficiency from coated MNs. Importantly, the results provide guidance for identifying critical attributes of the formulation, coating process and delivery device, that confer reproducible and effective delivery from coated MNs, and thus contribute to the requirements of the regulators appraising these devices
Observing Conditions at Mt.Graham: VATT UBVR Sky Surface Brightness and Seeing Measurements from 1999 through 2003
We present measurements of sky surface brightness and seeing on Mt.Graham
obtained at the Vatican Advanced Technology Telescope (VATT) during 16
observing runs between April 1999 and December 2003. We show that the sky
surface brightness is significantly darker during photometric conditions, and
can be highly variable over the course of a single observing run as well as
from one run to the next, regardless of photometricity. In our photometric
observations we find an average low-airmass (sec z < 1.2) sky surface
brightness of 22.00, 22.53, 21.49, and 20.88 mag arcsec^-2 in U, B, V, and R,
respectively. The darkest run (02/00 in U and 02/01 in BVR) had an average sky
surface brightness of 22.38, 22.86, 21.72, and 21.19 mag arcsec^-2 in U, B, V,
and R, respectively. With these results we show that under the best conditions,
Mt. Graham can compete with the darkest sites in Hawaii and Chile, thanks in
part to the strict dark-sky ordinances in place in Tucson and Safford. We
expect the sky over Mt. Graham to be even darker than our 1999--2003 results
during solar minimum (2006--2007). We find a significant improvement of about
0.45 arcsec in our measured stellar FWHM after improvements to the telescope
were made in Summer and Fall 2001. Stellar FWHM values are highly variable,
with median R-band focus FWHM values in each observing run ranging from 0.97
arcsec to 2.15 arcsec. Significantly sub-arcsecond seeing was occasionally
achieved with values as low as 0.65 arcsec FWHM in R. There may possibly still
be a significant telescope contribution to the seeing at the VATT, but nearby
trees as high as the dome are currently the dominant factor.Comment: 22 pages, 11 figures, to appear in PAS
Models and methods to characterise levonorgestrel release from intradermally administered contraceptives
Microneedle (MN)-based technologies have been proposed as a means to facilitate minimally invasive sustained delivery of long-acting hormonal contraceptives into the skin. Intradermal administration is a new route of delivery for these contraceptives and therefore no established laboratory methods or experimental models are available to predict dermal drug release and pharmacokinetics from candidate MN formulations. This study evaluates an in vitro release (IVR) medium and a medium supplemented with ex vivo human skin homogenate (SH) as potential laboratory models to investigate the dermal release characteristics of one such hormonal contraceptive that is being tested for MN delivery, levonorgestrel (LNG), and provides details of an accompanying novel two-step liquid–liquid drug extraction procedure and sensitive reversed-phase HPLC–UV assay. The extraction efficiency of LNG was 91.7 ± 3.06% from IVR medium and 84.6 ± 1.6% from the medium supplemented with SH. The HPLC–UV methodology had a limit of quantification of 0.005 µg/mL and linearity between 0.005 and 25 µg/mL. Extraction and detection methods for LNG were exemplified in both models using the well-characterised, commercially available sustained-release implant (Jadelle®). Sustained LNG release from the implant was detected in both media over 28 days. This study reports for the first time the use of biologically relevant release models and a rapid, reliable and sensitive methodology to determine release characteristics of LNG from intradermally administered long-acting drug delivery systems
Hydrodynamic gene delivery in human skin using a hollow microneedle device
Microneedle devices have been proposed as a minimally invasive delivery system for the intradermal administration of nucleic acids, both plasmid DNA (pDNA) and siRNA, to treat localised disease or provide vaccination. Different microneedle types and application methods have been investigated in the laboratory, but limited and irreproducible levels of gene expression have proven to be significant challenges to pre-clinical to clinical progression. This study is the first to explore the potential of a hollow microneedle device for the delivery and subsequent expression of pDNA in human skin. The regulatory approved MicronJet600® (MicronJet hereafter) device was used to deliver reporter plasmids (pCMVβ and pEGFP-N1) into viable excised human skin. Exogenous gene expression was subsequently detected at multiple locations that were distant from the injection site but within the confines of the bleb created by the intradermal bolus. The observed levels of gene expression in the tissue are at least comparable to that achieved by the most invasive microneedle application methods e.g. lateral application of a microneedle. Gene expression was predominantly located in the epidermis, although also evident in the papillary dermis. Optical coherence tomography permitted real time visualisation of the sub-surface skin architecture and, unlike a conventional intradermal injection, MicronJet administration of a 50 μL bolus appears to create multiple superficial microdisruptions in the papillary dermis and epidermis. These were co-localised with expression of the pCMVβ reporter plasmid. We have therefore shown, for the first time, that a hollow microneedle device can facilitate efficient and reproducible gene expression of exogenous naked pDNA in human skin using volumes that are considered to be standard for intradermal administration, and postulate a hydrodynamic effect as the mechanism of gene delivery
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