Novos registros dos lagartos Ameivula cipoensis Arias et al., 2014, Enyalius capetinga Breitman et al., 2018, Psilops paeminosus (Rodrigues, 1991) e Tupinambis quadrilineatus Manzani & Abe, 1997 (Squamata) para o estado de Minas Gerais, Brasil, através da ciência cidadã

Nós detectamos novas ocorrências para quatro espécies de lagartos no estado de Minas Gerais, Brasil: Ameivula cipoensis, Enyalius capetinga, Psilops paeminosus e Tupinambis quadrilineatus. Além disso, atualizamos o mapa de distribuição de todas as espécies com base em dados da literaturaAsociación Herpetológica Argentin

Molecular determinants of improved cathepsin B inhibition by new cystatins obtained by DNA shuffling

Background: Cystatins are inhibitors of cysteine proteases. The majority are only weak inhibitors of human cathepsin B, which has been associated with cancer, Alzheimer's disease and arthritis. Results: Starting from the sequences of oryzacystatin-1 and canecystatin-1, a shuffling library was designed and a hybrid clone obtained, which presented higher inhibitory activity towards cathepsin B. This clone presented two unanticipated point mutations as well as an N-terminal deletion. Reversing each point mutation independently or both simultaneously abolishes the inhibitory activity towards cathepsin B. Homology modeling together with experimental studies of the reverse mutants revealed the likely molecular determinants of the improved inhibitory activity to be related to decreased protein stability. Conclusion: A combination of experimental approaches including gene shuffling, enzyme assays and reverse mutation allied to molecular modeling has shed light upon the unexpected inhibitory properties of certain cystatin mutants against Cathepsin B. We conclude that mutations disrupting the hydrophobic core of phytocystatins increase the flexibility of the N-terminus, leading to an increase in inhibitory activity. Such mutations need not affect the inhibitory site directly but may be observed distant from it and manifest their effects via an uncoupling of its three components as a result of increased protein flexibility.State of Sao Paulo Research Foundation (FAPESP)[1998/14138-2]State of Sao Paulo Research Foundation (FAPESP)[05/59833-5]State of Sao Paulo Research Foundation (FAPESP)[08/58316-5

The use of Maytenus ilicifolia to prevent cisplatin-induced ototoxicity

Maytenus ilicifolia é uma planta sul americana apresenta várias propriedades medicinais, entre elas, a ação antioxidante. OBJETIVO: Por meio de um modelo original de ototoxicidade induzida pela cisplatina, verificar uma possível ação otoprotetora do extrato aquoso desta planta. MATERIAL E MÉTODO: Estudo clínico e experimental com cobaias fêmeas, albinas divididas em 5 grupos: 9 animais recebendo somente 3 doses de 7,5mg/kg/d do protocolo de cisplatina, 4 animais somente com o extrato, 10 animais com cisplatina e 1g/kg/d de extrato por 8 dias, 5 animais com cisplatina e 3g/kg/d do extrato por 8 dias e 5 animais recebendo extrato por 3 semanas e cisplatina na última semana. Os exames foram emissões otoacústica por produtos de distorção, potencial de tronco encefálico pré e após administração de cisplatina e, microscopia eletrônica de varredura. RESULTADOS: Os animais que receberam a cisplatina com o extrato, independente da dose, obtiveram alterações em todos os testes, com lesões na região basal na microscopia eletrônica. CONCLUSÃO: Apesar do efeito antioxidante da Maytenus ilicifolia, ela não foi suficiente para bloquear o efeito ototóxico da cisplatina.Maytenus ilicifolia is a native plant from South America, with several medicinal properties including antioxidant effects. AIM: using an original cisplatin induced ototoxicity model, we evaluated a possible otoprotection caused by Maytenus ilicifolia extract. MATERIALS AND METHODS: clinical and experimental study design with female albino guinea pigs divided in groups as follows: 9 animals receiving cisplatin only (three doses of 7.5mg/kg/day), 4 animals receiving the plant extract only, 10 animals receiving the cisplatin protocol and 1g/kg/day of extract for 8 days, 5 animals with cisplatin and 3g/kg/day of extract for 8 days, and 5 animals receiving extract for 3 weeks and cisplatin in the last week. The tests were distortion product otoacoustic emissions, brainstem auditory response, before and after medication and scanning electron microscopy. RESULTS: the animals receiving cisplatin plus plant extract, had alterations in all the tests, showing lesions on the basal cochlear region under electron microscopy. CONCLUSIONS: Despite of the plant extract's antioxidant effect, it was not sufficient to protect the cochlea against cisplatin ototoxicity

Effect of Gender, Energetics, and Biomechanics on Swimming Masters Performance

The purpose of this study was to analyze the effect of gender and energetics on biomechanics and performance of masters swimmers over 1 season. Twenty-five masters swimmers (14 male and 11 female) were assessed 3 times (TP1, TP2, and TP3) during a season (male personal record in 200-m freestyle event: 173.00 ± 31.41 seconds: female personal record in 200-m freestyle event: 200.73 ± 25.02 seconds). An incremental 5 × 200-m step test was selected to evaluate velocity at 4 mmol·l⁻¹ of blood lactate concentration (v4), maximal blood lactate concentration after exercise (La(peak)), maximal oxygen uptake (V̇O2max), stroke frequency, stroke length (SL), stroke index (SI), and propelling efficiency of the arm stroke (η(p)). The 200-m freestyle performance and average swimming velocity (v200) were also monitored. Significant differences were observed between males and females for the 200-m freestyle performance, SL, SI, and La(peak). Performance (205.18 ± 24.47 seconds; 197.45 ± 20.97 seconds; 193.45 ± 18.12 seconds), SL (1.69 ± 0.17 m; 1.79 ± 0.13 m; 1.78 ± 0.15 m), SI (1.68 ± 0.31 m²·c⁻¹·s⁻¹; 1.83 ± 0.27 m²·c⁻¹·s⁻¹; 1.85 ± 0.27 m²·c⁻¹·s⁻¹), η(p) (0.32 ± 0.04; 0.33 ± 0.03; 0.33 ± 0.04), and V̇O2max (38.71 ± 3.44 ml·kg⁻¹·min⁻¹; 43.43 ± 3.71 ml·kg⁻¹·min⁻¹; 43.95 ± 7.02 ml·kg⁻¹·min⁻¹) have changed significantly throughout the season (TP1, TP2, and TP3, respectively) in female swimmers. In male, significant changes were found in η(p) (0.33 ± 0.07; 0.36 ± 0.05; 0.36 ± 0.06) and V̇O2max (41.65 ± 7.30 ml·kg⁻¹·min⁻¹; 45.19 ± 6.55 ml·kg⁻¹·min⁻¹; 50.19 ± 9.65 ml·kg⁻¹·min⁻¹) over the season (TP1, TP2, and TP3, respectively). Gender presented a significant effect on SL (TP2: η(p)² = 0.29; TP3: η(p)² = 0.37), SI (TP2: η(p)² = 0.25), and La(peak) (TP3: η(p)² = 0.42). v4 (TP1: η(p)² = 0.23), SL (TP1: η(p)² = 0.46), SI (TP1: η(p)² = 0.78; TP2: η(p)² = 0.37; TP3: η(p)² = 0.32), and η(p) (TP1: η(p)² = 0.28) had a significant effect on performance. Male masters swimmers have better performance, SL, SI, and La(peak) than female counterparts. Female masters swimmers enhanced significantly the 200-m freestyle performance over the season due to the improvement in swimming technique (SL, SI, and η(p)) and energetic factors (v4 and V̇O2max). Nonsignificant improvements were observed for the males' performance. Gender has a significant effect on SL, SI, and La(peak). Therefore, performance is more dependent on technical factors than energetics.This work was supported by University of Beira Interior and Santander Totta bank (UBI/ FCSH/Santander/2010).info:eu-repo/semantics/publishedVersio

Aplicação de dados geofísicos na caracterização de falhas com potencial sismogénico no Vale Inferior do Tejo

O Vale Inferior do Tejo (VIT) tem sofrido os efeitos de sismos históricos. Algumas das fontes sismogénicas são conhecidas. Com vista a uma avaliação correcta das fontes sismogénicas em futuros estudos de perigosidade sísmica, integraram-se dados de reflexão sísmica, com dados de métodos potenciais e dados de epicentros relocalizados. As informações recolhidas a partir dos parâmetros das falhas irão ajudar à localização de fontes de sismos históricos utilizando elementos finitos de simulação de movimentos fortes

AGN feedback and star formation in the peculiar galaxy NGC 232: Insights from VLT-MUSE Observations

We use VLT-MUSE integral field unit data to study the ionized gas physical properties and kinematics as well as the stellar populations of the Seyfert 2 galaxy NGC\,232 as an opportunity to understand the role of AGN feedback on star formation. The data cover a field of view of 60$\times$60 arcsec$^{2}$ at a spatial resolution of $\sim$\,850\,pc. The emission-line profiles have been fitted with two Gaussian components, one associated to the emission of the gas in the disc and the other due to a bi-conical outflow. The spectral synthesis suggests a predominantly old stellar population with ages exceeding 2\,Gyr, with the largest contributions seen at the nucleus and decreasing outwards. Meanwhile, the young and intermediate age stellar populations exhibit a positive gradient with increasing radius and a circum-nuclear star forming ring with radius of $\sim$0.5\,kpc traced by stars younger than 20 Myr, is observed. This, along with the fact that AGN and SF dominated regions present similar gaseous oxygen abundances, suggests a shared reservoir feeding both star formation and the AGN. We have estimated a maximum outflow rate in ionized gas of $\sim$1.26\,M${\odot}$\,yr$^{-1}$ observed at a distance of $\sim$560 pc from the nucleus. The corresponding maximum kinetic power of the outflow is $\sim3.4\times10^{41}$ erg\,s$^{-1}$. This released energy could be sufficient to suppress star formation within the ionization cone, as evidenced by the lower star formation rates observed in this region.Comment: 14 pages,10 figures. Submitted to MNRA