Invited review: Authentication of milk by direct and indirect analysis of triacylglycerol molecular species.

Milk, milk-based products, and milk derivatives represent an important group of food commodities, with high nutritional value and widely consumed by large segment of consumers, including pregnant women, newborns, children, and the elderly. Food authentication is a rapidly growing field because of increasing consumer awareness regarding food quality and safety. This review attempts to critically summarize the status of direct and indirect analysis of the molecular species of triacylglycerols (TAG) used to assess the authenticity of milk. Identification and quantification of TAG molecular species in milk fat can be accurately performed even though analytical approaches focused on fraud evaluation should be developed. Recent analytical breakthroughs and novel techniques are discussed, along with their applications in milk authentication

Is the Household Microwave Recommended to Obtain Antioxidant-Rich Extracts from Lycium barbarum Leaves?

Nowadays, much interest is devoted to the extraction of plant materials, considering also their waste and by-products, to obtain antioxidant-rich products. The effect of household microwave-assisted extraction (MAE) on the phenolic content and antioxidant activity of Lycium barbarum leaf extracts was investigated. An experimental design approach was adopted considering solid/liquid ratio (1, 3, and 5 g of leaves in 150 mL water), irradiation time (1, 3, and 5 min), and microwave power (300, 400, and 500 W) as independent variables. These three factors and their interactions were studied to evaluate the effect of MAE conditions on the responses of total phenolic content, antioxidant activity, and chlorogenic acid content. The results showed that the analytical parameters were positively influenced by the solid/liquid ratio and time. On the contrary, microwave power was inversely correlated with the investigated responses. This research revealed that microwave extraction conditions should be carefully monitored to obtain bioactive-rich aqueous extracts with high antioxidant activity. A comparison with household traditional methods showed an unexpected lower phenolic content and antioxidant activity for MAE extract in respect to the decoction and infusion. In fact, it was found that L. barbarum leaf infusion had the best functional properties, regarding the investigated characteristics. The outcome of this study has implications for raising awareness that household preparation conditions strongly affect the health properties of herbal extracts

Phytochemical Analysis and Antiradical Properties of Sarcodon imbricatus (L.:Fr) Karsten:

The chemical composition of Sarcodon imbricatus (L.:Fr) Karsten (Hydnaceae) was evaluated to assess it as source of nutrients and nutraceuticals. The analyzed mushroom contains ergosterol and ergosterol peroxide. S. imbricatus methanolic extract showed a moderate antiradical activity (measured by DPPH radical scavenging activity). The combination of bioactive compounds and rich nutritional composition (high contents in protein, low content in fat, and its content of unsaturated fatty acids) makes the mushroom a good food

Italian Lycium barbarum L. Berry: Chemical Characterization and Nutraceutical Value:

Lycium barbarum L. has received considerable attention in recent years also in western countries because of the interesting healthy value of these berries. In this paper, goji samples cultivated in Southern Italy were analyzed for their chemical composition and nutritional profile in order to characterize fruits of Italian origin and to increase the awareness about their nutraceutical value. Lipid fraction was characterized by high percentages of unsaturated fatty acids, in particular oleic and linoleic acids, and very low values of atherogenic and thrombogenic indexes (0.1 and 0.2, respectively). In addition, goji berry was an interesting source of phytosterols (41.5 mg/100 g), essentially represented by β-sitosterol. Carotenoid analysis showed the presence of zeaxanthin, in esterified form, with high content of zeaxanthin dipalmitate (277.9 mg/100 g). Finally, in vitro antioxidant capacity and phenolic compounds were investigated. The results suggested that goji hydro-alcoholic extract possessed the ability to scavenge free radicals. Phenolic acids were clearly the most abundant compounds followed by flavonols and favanols. The results reported in this study confirm that Italian L. barbarum berry is a rich source of bioactive molecules with nutraceutical properties

Phenol Profiling and Nutraceutical Potential of Lycium spp. Leaf Extracts Obtained with Ultrasound and Microwave Assisted Techniques

In recent years, agricultural and industrial residues have attracted a lot of interest in the recovery of phytochemicals used in the food, pharmaceutical, and cosmetic industries. In this paper, a study on the recovery of phenol compounds from Lycium spp. leaves is presented. Ultrasound‐assisted extraction (UAE) and microwave‐assisted extraction (MAE) have been used with alcoholic and hydroalcoholic solvents. Methanolic UAE was the most successful technique for extracting phenols from Lycium leaves, and we used on leaves from L. barbarum and L. chinense cultivated in Italy. The extracts were then characterized as regards to the antioxidant properties by in vitro assays and the phenol profiling by a high performance liquid chromatography‐diode array detector (HPLC‐DAD). Chlorogenic acid and rutin were the main phenol compounds, but considerable differences have been observed between the samples of the two Lycium species. For example, cryptochlorogenic acid was found only in L. barbarum samples, while quercetin‐3‐O‐rutinoside‐7‐O‐glucoside and quercetin‐3‐O‐sophoroside‐7‐O‐rhamnoside only in L. chinense leaves. Finally, multivariate statistical analysis techniques applied to the phenol content allowed us to differentiate samples from different Lycium spp. The results of this study confirm that the extraction is a crucial step in the analytical procedure and show that Lycium leaves represent an interesting source of antioxidant compounds, with potential use in the nutraceutical field

In Vitro Safety/Protection Assessment of Resveratrol and Pterostilbene in a Human Hepatoma Cell Line (HepG2).

The aim of this work was to evaluate in vitro the genotoxic and/or antigenotoxic effects of resveratrol (RESV) and pterostilbene (PTER) on HepG2 cells. Moreover, additional tests were performed to evaluate early and late apoptosis events induced by the tested stilbenes. RESV and PTER did not show any genotoxic activity. As regards antigenotoxicity testing, RESV and PTER showed a typical, U-shaped hormetic dose-response relationship characterized by a biphasic trend with small quantities having opposite effects to large ones. HepG2 cells treated with PTER exhibited a marked increase in early apoptosis (40.1 %) at 250 μM; whereas, the highest concentration tested for both RESV and PTER significantly increased the proportion of HepG2 cells undergoing late apoptosis (32.5 and 51.2 %, respectively). The observed pro-apoptotic activity could, at least in part, explain the hormetic response observed when the compounds were tested for antigenotoxicity ( i.e., in the presence of induced DNA damage)

Seasonal variations in antioxidant compounds of <i>Olea europaea</i> leaves collected from different Italian cultivars

The objectives of this research were to study the best conditions for phenol extraction from olive tree leaves and to evaluate the content of phenolics and the antioxidant activity by in vitro assays in olive leaves harvested in different periods from four different Italian cultivars. The results showed that leaves/solvent ratio and temperature showed a significant and positive correlation with phenol extraction yield. In all harvesting periods the phenol content of Dolce Agogia samples was higher (P ≤ 0.05) with respect to Moraiolo, Leccino and Frantoio samples. The results of analysis by high-performance liquid chromatography coupled with diode array detector (HPLC-DAD) showed that Dolce Agogia had the highest concentrations of hydroxytyrosol and oleuropein. Moreover the highest contents of bioactives have been found in olive leaves harvested in December and March. In these months, corresponding to olive harvest and pruning of olive trees, leaves represent a waste product and this interesting result could be useful in the production of nutraceuticals

Phenol composition and antioxidant capacity of red wines produced in Central Italy changes after one-year storage

Much interest is currently concentrated on phenol compounds and antioxidants of wine. The aim of this study was to characterize and evaluate controlled designation of origin (CDO) and typical geographical indications (TGI) red wines from Central Italy and to evaluate possible modifications after one year of storage. The total phenol content and antioxidant activity by ORAC method were determined, while phenolic qualitative and quantitative profiles were evaluated by HRGC-FID or HPLC-DAD. All wines showed a good content of total phenols and an obvious antioxidant effect. After a one-year storage in the bottle, a significant decrease (P<0.05) of the ORAC values was observed for TGI wines. Interesting correlations between phenol and ORAC values for CDO wines were found. It can be confirmed that one-year storage in the bottle has not significantly affected the quality of the wines analyzed, in particular the CDO category

Special Issue on “Extraction Optimization Processes of Antioxidants”

Understanding the importance of extraction optimization processes of bioactive molecules in the food industry is fundamental in the choice of innovative extraction processes involving sustainable procedures and is essential in process design for the isolation of antioxidants, thus preserving their chemical integrity and biological properties [...

Varietal Authentication of Extra Virgin Olive Oils by Triacylglycerols and Volatiles Analysis

In recent years, there is an increasing interest in high-quality extra virgin olive oils (EVOOs) produced from local cultivars. They have particular chemical/organoleptic characteristics and are frequently subjected to fraud, whereby the control of quality requires a powerful varietal check. In the present research, triacylglycerols (TAGs) and volatiles have been studied as chemical markers for the authentication of EVOO samples from four Italian varieties of Olea europea (Dolce Agogia, Frantoio, Leccino, and Moraiolo). The monocultivar EVOO samples have been subjected to a chemical–enzymatic chromatographic method in order to perform a stereospecific analysis, an important procedure for the characterization of TAG of food products. The results, combined with chemometric analysis (linear discriminant analysis, LDA), were elaborated in order to classify Italian EVOO monocultivar samples. In accordance with the total and intrapositional fatty acid (FA) composition of TAG fraction, the results were allowed to carry out a varietal discrimination. In addition, volatile compounds were also determined by solid-phase micro-extraction gas chromatography-mass spectrometry analysis. All EVOO samples were correctly classified when TAG stereospecific data and volatile results were elaborated by the LDA procedure, even if volatile compounds showed a higher discriminant power