A new Approach for Structure from Motion Underwater Pile-Field Documentation

For a pilot study carried out by the University of Bern together with local partners in Summer 2018 at the pile-dwelling site Bay of Bones (Rep. of Macedonia), a new workflow for underwater pile-field documentation was developed. The site lies in shallow water of 3–5 meters depth and the most obvious constructive remains of the prehistoric settlement are thousands of wooden piles. The piles, mainly of oak and juniper, are excellently preserved in the lake sediments. The aim of the project was to document and sample 40 m2 surface area of the pile-field and the dendrochronological analysis of the samples. Dendrochronological sampling requires cutting the top-ends of the piles and thus changes the preserved situation. Therefore beforehand documentation must ensure the localization of each pile on a map. This calls for a method that ensures a) that every pile is distinctly labeled and b) the location of each pile is accurately captured. While on land, this can easily be achieved, underwater working conditions complicate common procedures. E.g. by measuring with a folding ruler from a local grid, there is later no way to evaluate measuring mistakes or the internal error of the local grid. In addition, for unpracticed divers measuring by hand underwater is not only time-consuming but also tends a lot more to erroneous results than on land. The goal was therefore to find a time-saving, accurate and easy to carry out way to locate the positions of several hundred piles in shallow water. The best solution for us to achieve these goals was a new standardized and reproducible workflow with Structure from Motion (SfM). The applied approach for underwater SfM-documentation includes on-site workflow and post-processing. The on-site workflow covers all steps from the preparation of the archaeological structures to the photographic data acquisition, the calculation of a preliminary 3D-model and its on-site verification. The crucial step was to ensure the suitability for modeling of the data before the situation underwater was irreversibly changed through sampling. Post-processing was carried out in Adobe Photoshop, Agisoft PhotoScan and QGIS where the data was optimized in quality and standardized from digital image processing to the construction of a georeferenced orthomosaic. Applying these results, we can later visualize patterns in the spatial distribution of the piles concerning e.g. their age, their size or their wood species. This will lead to answers regarding architecture, internal chronology, and in-site settlement dynamics. With this newly standardized two-step-workflow for underwater structure documentation, we are able to asses and compare the quality of each orthomosaic in a reproducible way. The presented method is highly promising for underwater-documentation of prehistoric pile-fields, yielding accurate digital plans in an efficient and cost-saving way.</p

Capacité d’innovation des entreprises agroalimentaires et insertion dans les réseaux : le rôle de la proximité organisationnelle

Cet article s’interroge sur le rôle de la proximité dans le processus de transfert de connaissances et le potentiel d’innovation des entreprises en s’appuyant sur des données issues d’une enquête directe auprès de 41 entreprises agroalimentaires bourguignonnes. Différents types d’entreprises sont distinguées en fonction de leur potentiel interne et des réseaux d’innovation dans lesquels ces entreprises sont insérées. Au-delà de la proximité géographique, la proximité organisationnelle apparaît comme un facteur essentiel dans la mise en relation entre entreprise et centres scientifiques et techniques.This article discusses the role of proximity in the process of knowledge transfer and in determining the innovation capacity of firms, using data from a direct survey of 41 agro-food firms in Burgundy. The authors identify different types of firms according to their internal potential and the networks they belong to. Beyond the geographical proximity, organizational proximity is essential for relationships between firms and scientific and technical centres

Biologicals and Fetal Cell Therapy for Wound and Scar Management

Few biopharmaceutical preparations developed from biologicals are available for tissue regeneration and scar management. When developing biological treatments with cellular therapy, selection of cell types and establishment of consistent cell banks are crucial steps in whole-cell bioprocessing. Various cell types have been used in treatment of wounds to reduce scar to date including autolog and allogenic skin cells, platelets, placenta, and amniotic extracts. Experience with fetal cells show that they may provide an interesting cell choice due to facility of outscaling and known properties for wound healing without scar. Differential gene profiling has helped to point to potential indicators of repair which include cell adhesion, extracellular matrix, cytokines, growth factors, and development. Safety has been evidenced in Phase I and II clinical fetal cell use for burn and wound treatments with different cell delivery systems. We present herein that fetal cells present technical and therapeutic advantages compared to other cell types for effective cell-based therapy for wound and scar management

Lifestyle Behaviours and Plasma Vitamin C and β-Carotene Levels from the ELAN Population (Liège, Belgium)

Several factors, including fruit and vegetables intakes, have been shown to significantly influence the plasma concentrations of the two antioxidants vitamin C and β-carotene. Deficiency levels of 6 mg/L (34.2 μM) for vitamin C and of 0.22 mg/L (0.4 μM) for β-carotene have been suggested below which cardiovascular risk might be increased. The present study performed on 897 presumably healthy subjects aged 40–60 years aimed to examine how modifiable lifestyle factors may be related to vitamin C and/or β-carotene deficiency. Gender, smoking, lack of regular physical activity and of daily fruit consumption (≥2/day), and social status (in particular, unemployment) were found to be significant risk factors for vitamin C deficiency. For β-carotene deficiency, the same factors were identified except social status; moreover, overweight and OC use in women were also found to have a deleterious effect. For non exposed subjects, the probability of developing vitamin C deficiency was 4% in men and 2.4% in women. This probability increased to 66.3% for men and to 44.3% for women (and even to 50.4% under OC use), when all risk factors were present. For β-carotene deficiency, the corresponding probabilities were equal to 29.7% in men and 13.7% in women (no risk factor present), and to 86.1% for men and 69.9% (91.6% for OC use) for women (all factors present), respectively

Identifying Changepoints in Biomarkers During the Preclinical Phase of Alzheimer’s Disease

Objective: Several models have been proposed for the evolution of Alzheimer’s disease (AD) biomarkers. The aim of this study was to identify changepoints in a range of biomarkers during the preclinical phase of AD.Methods: We examined nine measures based on cerebrospinal fluid (CSF), magnetic resonance imaging (MRI) and cognitive testing, obtained from 306 cognitively normal individuals, a subset of whom subsequently progressed to the symptomatic phase of AD. A changepoint model was used to determine which of the measures had a significant change in slope in relation to clinical symptom onset.Results: All nine measures had significant changepoints, all of which preceded symptom onset, however, the timing of these changepoints varied considerably. A single measure, CSF t-tau, had an early changepoint (34 years prior to symptom onset). A group of measures, including the remaining CSF measures (CSF Abeta and phosphorylated tau) and all cognitive tests had changepoints 10–15 years prior to symptom onset. A second group is formed by medial temporal lobe shape composite measures, with a 6-year time difference between the right and left side (respectively nine and 3 years prior to symptom onset).Conclusion: These findings highlight the long period of time prior to symptom onset during which AD pathology is accumulating in the brain. There are several significant findings, including the early changes in cognition and the laterality of the MRI findings. Additional work is needed to clarify their significance

Exploring common genetic contributors to neuroprotection from amyloid pathology

Preclinical Alzheimer’s disease describes some individuals who harbor Alzheimer’s pathologies but are asymptomatic. For this study, we hypothesized that genetic variation may help protect some individuals from Alzheimer’s-related neurodegeneration. We therefore conducted a genome-wide association study using 5,891,064 common variants to assess whether genetic variation modifies the association between baseline beta-amyloid, as measured by both cerebrospinal fluid and positron emission tomography, and neurodegeneration defined using MRI measures of hippocampal volume. We combined and jointly analyzed genotype, biomarker, and neuroimaging data from non-Hispanic white individuals who were enrolled in four longitudinal aging studies (n=1065). Using regression models, we examined the interaction between common genetic variants (Minor Allele Frequency > 0.01), including APOE-ε4 and APOE-ε2, and baseline cerebrospinal levels of amyloid (CSF Aβ42) on baseline hippocampal volume and the longitudinal rate of hippocampal atrophy. For targeted replication of top findings, we analyzed an independent dataset (n=808) where amyloid burden was assessed by Pittsburgh Compound B ([{11}^C]-PiB) PET. In this study, we found that APOE-ε4 modified the association between baseline CSF Aβ42 and hippocampal volume such that APOE-ε4 carriers showed more rapid atrophy, particularly in the presence of enhanced amyloidosis. We also identified a novel locus on chromosome 3 that interacted with baseline CSF Aβ42. Minor allele carriers of rs62263260, an expression quantitative trait locus for the SEMA5B gene, (p=1.46x10^{-8}; 3:122675327) had more rapid neurodegeneration when amyloid burden was high and slower neurodegeneration when amyloid was low. The rs62263260 x amyloid interaction on longitudinal change in hippocampal volume was replicated in an independent dataset (p=0.0112) where amyloid burden was assessed by PET. In addition to supporting the established interaction between APOE and amyloid on neurodegeneration, our study identifies a novel locus that modifies the association between beta-amyloid and hippocampal atrophy. Annotation results may implicate SEMA5B, a gene involved in synaptic pruning and axonal guidance, as a high-quality candidate for functional confirmation and future mechanistic analysis