Dimensionless Numbers Reveal Distinct Regimes in the Structure and Dynamics of Pedestrian Crowds

In fluid mechanics, dimensionless numbers like the Reynolds number help classify flows. We argue that such a classification is also relevant for crowd flows by putting forward the dimensionless Intrusion and Avoidance numbers.Using an extensive dataset, we show that these delineate regimes that are characterized by distinct structural signatures, best probed in terms of distances at low Avoidance number and times-to-collision at low Intrusion number.These findings prompt a perturbative expansion of the agent-based dynamics; the generic models thus obtained perform well in (and only in) the regime in which they were derived

Single-file pedestrian dynamics: a review of agent-following models

Single-file dynamics has been studied intensively, both experimentally and theoretically. It shows interesting collective effects, such as stop-and-go waves, which are validation cornerstones for any agent-based modeling approach of traffic systems. Many models have been proposed, e.g. in the form of car-following models for vehicular traffic. These approaches can be adapted for pedestrian streams. In this study, we delve deeper into these models, with particular attention on their interconnections. We do this by scrutinizing the influence of different parameters, including relaxation times, anticipation time, and reaction time. Specifically, we analyze the inherent fundamental problems with force-based models, a classical approach in pedestrian dynamics. Furthermore, we categorize car-following models into stimulus-response and optimal velocity models, highlighting their historical and conceptual differences. These classes can further be subdivided considering the conceptual definitions of the models, e.g. first-order vs. second-order models, or stochastic vs. deterministic models with and without noise. Our analysis shows how car-following models originally developed for vehicular traffic can provide new insights into pedestrian behavior. The focus on single-file motion, which is similar to single-lane vehicular traffic, allows for a detailed examination of the relevant interactions between pedestrians.Comment: 35 pages, 10 Figures; chapter accepted for publication in Crowd Dynamics (vol. 4

Mechanisms and advancement of antifading agents for fluorescence microscopy and single-molecule spectroscopy

Modern fluorescence microscopy applications go along with increasing demands for the employed fluorescent dyes. In this work, we compared antifading formulae utilizing a recently developed reducing and oxidizing system (ROXS) with commercial antifading agents. To systematically test fluorophore performance in fluorescence imaging of biological samples, we carried out photobleaching experiments using fixed cells labeled with various commonly used organic dyes, such as Alexa 488, Alexa 594, Alexa 647, Cy3B, ATTO 550, and ATTO 647N. Quantitative evaluation of (i) photostability, (ii) brightness, and (iii) storage stability of fluorophores in samples mounted in different antifades (AFs) reveal optimal combinations of dyes and AFs. Based on these results we provide guidance on which AF should preferably be used with a specific dye. Finally, we studied the antifading mechanisms of the commercial AFs using single-molecule spectroscopy and reveal that these empirically selected AFs exhibit similar properties to ROXS AFs

Design of a Software Tool Supporting Orientation in the Context of Digital Transformation

The digital transformation is a continuous and complex but indispensable endeavor. Because practice and academia regularly develop new digital technologies, companies face difficulties in selecting appropriate technologies to innovate their business model. Step-by-step instructions may guide companies to identify, evaluate, and select potential digitalization ideas. Software tools can support these activities in a structured way. Thus, the research objective of this paper is to design a software tool supporting the orientation phase of a company's digital transformation. To reach this objective, we conduct an action design research approach in cooperation with three companies. As an evaluation step, a focus group discussion with practitioners and end-users is carried out. The final tool design comprises 18 design principles that serve as the basis for a set of visual mockups and a prototype implementation of the intended tool

A Procedure Model for Integrating Retailers into Digital Platforms

The integration into digital platforms is a vital countermeasure for retailers to counter rising customer expectations, emerging competition, and rapid technological developments. To break down the behemoth of integration projects into smaller steps, we synthesized a procedure model incorporating requirements from scientific literature and practice. We applied design science research to derive a well-structured and scientifically sound artifact that was evaluated in eleven interviews with practitioners. Our analysis revealed that integrating retailers into digital platforms can be standardized for different types and depths following specific guidelines outlined in the proposed procedure model of this work. Furthermore, the integration shows many similarities to other domains, especially when considering small- and medium-sized organisations. However, there are some peculiarities for retailers that could rationalize a distinct view on the integration

ColiCoords: A Python package for the analysis of bacterial fluorescence microscopy data

Single-molecule fluorescence microscopy studies of bacteria provide unique insights into the mechanisms of cellular processes and protein machineries in ways that are unrivalled by any other technique. With the cost of microscopes dropping and the availability of fully automated microscopes, the volume of microscopy data produced has increased tremendously. These developments have moved the bottleneck of throughput from image acquisition and sample preparation to data analysis. Furthermore, requirements for analysis procedures have become more stringent given the demand of various journals to make data and analysis procedures available. To address these issues we have developed a new data analysis package for analysis of fluorescence microscopy data from rod-like cells. Our software ColiCoords structures microscopy data at the single-cell level and implements a coordinate system describing each cell. This allows for the transformation of Cartesian coordinates from transmission light and fluorescence images and single-molecule localization microscopy (SMLM) data to cellular coordinates. Using this transformation, many cells can be combined to increase the statistical power of fluorescence microscopy datasets of any kind. ColiCoords is open source, implemented in the programming language Python, and is extensively documented. This allows for modifications for specific needs or to inspect and publish data analysis procedures. By providing a format that allows for easy sharing of code and associated data, we intend to promote open and reproducible research. The source code and documentation can be found via the project’s GitHub page

A facile synthesis of Robinson’s NHC-stabilised diborane(4)

Reactions of bis(pinacolato)diboron (B2pin2) with [(IDip)AlH3] (IDip = (HCNDip)2C:, Dip = 2,6-iPr2C6H3) afforded both the new diborane [(IDip)BH2B(pin)] and the known compound [(IDip)BH2BH2(IDip)] in a facile one-pot procedure; the latter reaction is improved by the addition of free IDip. [(IDip)AlH3] transfers both IDip and hydride ligands to the diborane moiety in a halide-free approach. [(IDip)BH2B(pin)] was structurally and spectroscopically characterised and additional spectroscopic data for [(IDip)BH2BH2(IDip)] is reported.Publisher PDFPeer reviewe

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Si la adolescencia fue descubierta a finales del siglo XIX, y se democratizó en la primera mitad del XX, la segunda mitad del siglo ha presenciado la irrupción de la juventud, ya no como sujeto pasivo sino como actor protagonista en la escena pública