Landowners\u27 Perceptions of Conservation Easements: Implications for Effective Persuasive Communication

Conservation easements are one of the most popular voluntary, legally binding programs that allow individual landowners to use their property to protect the environment. There is a definite need for educational and persuasive messaging targeted towards landowners regarding adopting conservation easements. There is currently little to no existing literature specifically related to the attitudes and behaviors landowners possess towards conservation easements. This study aimed to fill a gap in literature by gathering information from landowners in Northwest Arkansas and Northeast Oklahoma, using a quantitative survey. Respondents were asked to identify their awareness-level knowledge about conservation easements, as well as rank incentivizing and disincentivizing factors that would influence their decision to adopt conservation easements. Respondents were also asked questions regarding their communications preferences. Researchers identified that there is a strong need for regional easement organizations to increase their awareness-level educational communications efforts. In addition, participants noted a strong desire for more personalized digital and unstructured in-person communications methods to be used by easement organizations

Landowners\u27 Perceptions of Conservation Easements: Implications for Effective Persuasive Communication

Conservation easements are one of the most popular voluntary, legally binding programs that allow individual landowners to use their property to protect the environment. There is a definite need for educational and persuasive messaging targeted towards landowners regarding adopting conservation easements. There is currently little to no existing literature specifically related to the attitudes and behaviors landowners possess towards conservation easements. This study aimed to fill a gap in literature by gathering information from landowners in Northwest Arkansas and Northeast Oklahoma, using a quantitative survey. Respondents were asked to identify their awareness-level knowledge about conservation easements, as well as rank incentivizing and disincentivizing factors that would influence their decision to adopt conservation easements. Respondents were also asked questions regarding their communications preferences. Researchers identified that there is a strong need for regional easement organizations to increase their awareness-level educational communications efforts. In addition, participants noted a strong desire for more personalized digital and unstructured in-person communications methods to be used by easement organizations

Consuming transgenic goats' milk containing the antimicrobial protein lysozyme helps resolve diarrhea in young pigs.

Childhood diarrhea is a significant problem in many developing countries and E. coli is a main causative agent of diarrhea in young children. Lysozyme is an antimicrobial protein highly expressed in human milk, but not ruminant milk, and is thought to help protect breastfeeding children against diarrheal diseases. We hypothesized that consumption of milk from transgenic goats which produce human lysozyme (hLZ-milk) in their milk would accelerate recovery from bacterial-induced diarrhea. Young pigs were used as a model for children and infected with enterotoxigenic E. coli. Once clinical signs of diarrhea developed, pigs were fed hLZ-milk or non-transgenic control goat milk three times a day for two days. Clinical observations and complete blood counts (CBC) were performed. Animals were euthanized and samples collected to assess differences in histology, cytokine expression and bacterial translocation into the mesenteric lymph node. Pigs consuming hLZ-milk recovered from clinical signs of infection faster than pigs consuming control milk, with significantly improved fecal consistency (p = 0.0190) and activity level (p = 0.0350). The CBC analysis showed circulating monocytes (p = 0.0413), neutrophils (p = 0.0219), and lymphocytes (p = 0.0222) returned faster to pre-infection proportions in hLZ-milk fed pigs, while control-fed pigs had significantly higher hematocrit (p = 0.027), indicating continuing dehydration. In the ileum, pigs fed hLZ-milk had significantly lower expression of pro-inflammatory cytokine IL-8 (p = 0.0271), longer intestinal villi (p<0.0001), deeper crypts (p = 0.0053), and a thinner lamina propria (p = 0.0004). These data demonstrate that consumption of hLZ-milk helped pigs recover from infection faster, making hLZ-milk an effective treatment of E. coli-induced diarrhea

Investigations of Personalized Medicine in Mesothelioma

Malignant mesothelioma is a neoplasm that involves lesions on the pleural linings of the lung or the peritoneal lining of the abdominal cavity. Current standard of care involves a multi-targeted antifolate drug Pemetrexid and an alylating agent, Cisplatin. This regimen results in regression of tumors in 25% of patients. As the genetics lesions that cause mesothelioma have been elucidated, our understanding of possible targets of therapeutics has grown. Rational drug design (personalized medicine) would dictate that we consider genetic alterations specific to mesothelioma tumor cells and use those to target our therapy. The most common genetic alterations in human mesotheliomas are the loss of function of three tumor suppressor genes, PTEN, NF2, and CDKN2A.In this project, we propose to induce genomic deletion of the PTEN locus by using CRISPR/Cas9 nuclease-generating lentiviral vectors targeting this site in a “normal” mesothelial cell model, LP9/hTert. As we complete the establishment of this model system with various genomic alterations, the model system will be used to test a small set of frontline chemotherapeutic agents for any increased therapeutic index, the ratio of tumor cell killing to toxicity of normal cells. Theoretically, genes and proteins downstream of these genetic lesions will make some cancer cells more susceptible to specific inhibitors. Among the drugs to be tested are novel PI3Kinase and mTOR inhibitors. Our hypothesis is that PTEN knockout by CRISPR/Cas9 editing will result in an increase in sensitivity of suppressed cells to novel inhibitors directed at the pathways affected. To confirm genomic alteration, sequencing of the affected locus has been completed, other assays are planned to complete the confirmation of PTEN knockout. These include, quantitative PCR of PTEN mRNA, Immunoblotting to assess protein status, and functional analysis of PTEN function

Landowners\u27 Perceptions of Conservation Easements: Implications for Effective Persuasive Communication

Conservation easements (CEs) are one of the most popular voluntary, legally binding programs that allow individual landowners to use their property to protect the environment. There is a definite need for educational and persuasive messaging targeted towards landowners regarding adopting conservation easements. Based on previous studies, and confirmed by this study, communication efforts that are led by conservation experts who are also community members are most likely to be well received by landowners in a region. There is currently little to no existing region-specific literature specifically related to the attitudes and behaviors landowners possess towards conservation easements. Additionally, more literature is needed regarding landowners’ communications preferences for learning about CEs; the existing literature focuses broadly on environmental communication. This study aimed to fill a gap in literature by conducting a quantitative survey and qualitative phone interview with landowners in Northwest Arkansas and Northeast Oklahoma. Participants were asked to identify their awareness level knowledge about conservation easements, as well as rank incentivizing and disincentivizing factors that would influence their decision to adopt conservation easements. Researchers identified that there is a strong need for regional easement organizations to increase their awareness-level educational communications efforts. In addition, participants noted a strong desire for more personalized and in-person communications methods to be used by easement organizations

Caldwell-Dennis House Adaptive Reuse Feasibility Study

Professor JP Halls ARCH 607- Historic Preservation Studio course created an adaptive reuse plan to examine the feasibility of rehabilitating the historic Caldwell-Dennis residence into transitional housing for women reentering society

Exploration of the polymorphic solid-state landscape of an amide-linked organic cage using computation and automation

Organic cages can possess complex, functionalised internal cavities that make them promising candidates for synthetic enzyme mimics. Conformationally flexible but chemically robust structures are needed for adaptable guest binding and catalysis, but these rapidly exchanging systems are difficult to resolve in solution. Here, we use inexpensive calculations and high-throughput crystallisation experiments to identify accessible cage conformations for a recently reported organic cage by ‘locking’ them in the solid state. The conformers identified exhibit a range of distances between the carboxylic acid groups in the internal cavity, suggesting adaptability towards binding a wide array of target guest molecules. The complexity of the observed crystal structures goes beyond what is possible with state-of-the-art crystal structure prediction

Genome editing in poultry - opportunities and impacts

Poultry products (meat and eggs) are a major source of animal protein on which the world is increasingly reliant to feed a rapidly growing population. Improved breeds and advances in farm management practices have had a large impact on the poultry industry. For example, using current genetic stock and production practices, broiler chickens can weigh 2 kg in about 34 days. Forty-five years ago it would have typically taken over 60 days. These impressive advances have been made using traditional selective breeding methods and more recently by using genomics. Now, with the availability of precision genome engineering tools there are new opportunities to improve poultry production above and beyond those achievable by traditional means. One major opportunity is disease resilience, particularly for viral diseases such as avian influenza that has devastating impacts on the poultry industry. Resilience to specific diseases can be a notoriously difficult trait to select for using traditional breeding and the latest technologies that precisely edit the genome have created new ways to address this challenge

A Mixture of LBG Overdensities in the Fields of Three 6<z<76 < z < 7 Quasars: Implications for the Robustness of Photometric Selection

The most luminous quasars at $z > 6$ are suspected to be both highly clustered and reside in the most massive dark matter halos in the early Universe, making them prime targets to search for galaxy overdensities and/or protoclusters. We search for Lyman-break dropout-selected galaxies using HST WFC3/ACS broadband imaging in the fields of three $6 < z < 7$ quasars, as well as their simultaneously observed coordinated-parallel fields, and constrain their photometric redshifts using EAZY. One field, J0305-3150, shows a volume density 10$\times$ higher than the blank-field UV luminosity function (UVLF) at M$_{UV} < -20$, with tentative evidence of a 3$\sigma$ overdensity in its parallel field located 15 cMpc away. Another field, J2054-0005, shows an angular overdensity within 500 ckpc from the quasar but still consistent with UVLF predictions within 3$\sigma$, while the last field, J2348-3054, shows no enhancement. We discuss methods for reducing uncertainty in overdensity measurements when using photometric selection and show that we can robustly select LBGs consistent with being physically associated with the quasar, corroborated by existing JWST/NIRCam WFSS data in the J0305 field. Even accounting for incompleteness, the overdensities in J0305 and J2054 are higher for brighter galaxies at short angular separations, suggesting preferential enhancement of more massive galaxies in the immediate vicinity of the quasar. Finally, we compare the LBG population with previously-identified [CII] and mm-continuum companions; the LBG overdensities are not accompanied by an enhanced number of dusty galaxies, suggesting that the overdense quasar fields are not in the bursty star-forming phase sometimes seen in high-redshift protoclusters.Comment: 22 pages (main text), 12 figures, 10 tables, 2 appendices. Final version after addressing referee report, accepted to ApJ May 202

Searching Far and Long I: Pilot ALMA 2mm Follow-up of Bright Dusty Galaxies as a Redshift Filter

A complete census of dusty star-forming galaxies (DSFGs) at early epochs is necessary to constrain the obscured contribution to the cosmic star formation rate density (CSFRD), however DSFGs beyond $z \sim 4$ are both rare and hard to identify from photometric data alone due to degeneracies in submillimeter photometry with redshift. Here, we present a pilot study obtaining follow-up Atacama Large Millimeter Array (ALMA) $2\,$mm observations of a complete sample of 39 $850\,\rm\mu m$-bright dusty galaxies in the SSA22 field. Empirical modeling suggests $2\,$mm imaging of existing samples of DSFGs selected at $850\,\rm\mu m - 1\,$mm can quickly and easily isolate the "needle in a haystack" DSFGs that sit at $z>4$ or beyond. Combining archival submillimeter imaging with our measured ALMA $2\,$mm photometry ($1\sigma \sim 0.08\,$mJy$\,$beam$^{-1}$ rms), we characterize the galaxies' IR SEDs and use them to constrain redshifts. With available redshift constraints fit via the combination of six submillimeter bands, we identify 6/39 high-$z$ candidates each with $>50\%$ likelihood to sit at $z > 4$, and find a positive correlation between redshift and $2\,$mm flux density. Specifically, our models suggest the addition of $2\,$mm to a moderately constrained IR SED will improve the accuracy of a millimeter-derived redshift from $\Delta z/(1+z) = 0.3$ to $\Delta z/(1+z) = 0.2$. Our IR SED characterizations provide evidence for relatively high emissivity spectral indices ($\langle \beta \rangle = 2.4\pm0.3$) in the sample. We measure that especially bright ($S_{850\rm\mu m}>5.55\,$mJy) DSFGs contribute $\sim10$% to the cosmic-averaged CSFRD from $2<z<5$, confirming findings from previous work with similar samples.Comment: 22 pages, 7 figures, accepted for publication in Ap