ASSESSMENT OF MANUFACTURABILITY AND PERFORMANCE OF POLYURETHANE HEART VALVES PRODUCED THROUGH A LOCALLY DEVELOPED DIP MOULDING PROCESS

Conference ProceedingsPolyurethane heart valves have been widely studied as possible replacement for mechanical and bioprosthetic heart valves. The development of an inexpensive routine production technique for manufacturing of polyurethane valves will greatly benefit a very large number of patients in developing and emerging countries. A polymer heart valve shows favourable physical properties and flow dynamics compared to human heart valves, however, the outcome of producing a polymer heart valve with the required flexibility, durability and hemodynamic function is often difficult to predict. The design of the mould, the selection of the material and the fabrication method used are the key factors that influence the achievement of an acceptable heart valve for use in the human body. From their previous work on developing a repeatable, semi-automated dip moulding process for producing tri-leaflet polyurethane heart valves, the authors have shown that the selection of an appropriate set of dip moulding process parameters and mould material properties could result in achieving polyurethane valve leaflets with the required physical and mechanical properties. This paper reports on the progress made with application of the developed dip moulding process to produce polyurethane heart valves suitable for use in human body. The mould, frame and sewing ring were manufactured in Ti6Al4V(ELI) by using a Direct Metal Laser Sintering (DMLS) process and the valve leaflets were moulded directly onto the sewing ring. The heart valve properties obtained are presented and assessed. Conclusions are drawn regarding the prospects of these valves surviving the extensive in vitro simulation trials required to qualify them for subsequent clinical trials

Validation of University of New Brunswick Ionospheric Modeling Technique with ionosonde TEC estimation over South Africa

For more than a decade, ionospheric research over South Africa has been carried out using data from ionosondes geographically located at Madimbo (28.38°S, 30.88°E), Grahamstown (33.32°S, 26.50°E), and Louisvale (28.51°S, 21.24°E). The objective has been modelling the bottomside ionospheric characteristics using neural networks. The use of Global Navigation Satellite System (GNSS) data is described as a new technique to monitor the dynamics and variations of the ionosphere over South Africa, with possible future application in high frequency radio communication. For this task, the University of New Brunswick Ionospheric Modelling Technique (UNB-IMT) was applied to compute midday (10:00 UT) GNSS-derived total electron content (GTEC). GTEC values were computed using GNSS data for stations located near ionosondes for the years 2002 and 2005 near solar maximum and minimum, respectively. The GTEC was compared with the midday ionosonde-derived TEC (ITEC) measurements to validate the UNB-IMT results. It was found that the variation trends of GTEC and ITEC over all stations are in good agreement and show a pronounced seasonal variation for the period near solar maximum, with maximum values ( 80 TECU) around autumn and spring equinoxes, and minimum values ( 22 TECU) around winter and summer. Furthermore, the residual ΔTEC = GTEC − ITEC was computed. It was evident that ΔTEC, which is believed to correspond to plasmaspheric electron content, showed a pronounced seasonal variation with maximum values ( 20 TECU) around equinoxes and minimum ( 5 TECU) around winter near solar maximum. The equivalent ionospheric and total slab thicknesses were also computed and comprehensively discussed. The results verified the use of UNB-IMT as one of the tools for future ionospheric TEC research over South Africa

Thermal analysis of the LLR optical telescope tube assembly based in Hartebeesthoek Radio Astronomy Observatory

The Hartebeesthoek Radio Astronomy Observatory of South Africa is currently developing a lunar laser ranger (LLR) system based on a one metre aperture telescope in collaboration with National Aeronautics and Space Administration and the Observatoire de la Côte d’Azur. This LLR will be an addition to a limited list of operating LLR stations globally and it is expected to achieve sub-centimetre range precision to the Moon. Key to this expectation including the overall telescope operational performance is thermal analysis of the telescope structure, based on the thermal properties of component materials and their interaction with the environment through conventional heat transfer mechanisms. This paper presents transient thermal simulation results of the telescope’s optical tube and one metre primary mirror in terms of thermal variations and consequent structural deformations. The results indicate that on a non-windy, cloud-free and winter day, the temperature gradients on the structure could be within 1 °C with respect to the temporal ambient air temperatures at the site when these are between 9 and 23 °C. Furthermore, these gradients were coupled with thermally-induced total deformations that vary between 2.9 and 40.7 μm of the assembled telescope components. In overall, these findings suggest that both the tube and especially the mirror may respond very slowly to ambient temperatures; however, correcting for structural thermal variations is imperative in maximizing the pointing accuracy of the telescope thereby increasing the chance being on-target with the retroreflectors located on the Moon surface.P. L. Tsela hereby acknowledges research support in part by the National Research Foundation of South Africa for the grant, Unique Grant No. 93952.http://link.springer.com/journal/403282017-09-30hb2016Geography, Geoinformatics and Meteorolog

Analysis of the performance of hydrogen maser clocks at the Hartebeesthoek Radio Astronomy Observatory

Hydrogen maser frequency standards are commonly utilised in various space geodetic techniques such as Very Long Baseline Interferometry (VLBI) as local reference clocks. The Hartebeesthoek Radio Astronomy Observatory in South Africa is currently operating two maser frequency standards i.e., an EFOS28 and an iMaser72 for the 15 m and 26 m VLBI radio telescopes respectively, an older EFOS6 is a standby spare. This study utilised the least-squares method to derive clock parameters, which indicates the performance levels of the masers by making use of the offset measurements obtained between hydrogen maser clock 1 PPS and GNSS 1 PPS for a period of 35 days. The masers were also compared using a frequency comparator (VCH-314) for a time period of 100 s. The results indicate that the performances of both Masers are relatively similar to each other, with short-term and long-term results indicating good agreement. The iMaser72 has a better standard error of 0.0039 μs compared to the standard error of 0.0059 μs for the EFOS28 maser clock. In general, both masers performed at an expected level required for radio astronomy and geodetic VLBI applications. The method used in this study proved to be useful in managing local hydrogen maser clocks to ensure accurate VLBI observations are obtained

Mapping GPS-derived ionospheric Total Electron Content over Southern Africa during different epochs of solar cycle 23

The Southern African Development Community and the International Global Navigation Satellite Systems Service (GNSS) network of dual frequency Global Positioning System (GPS) receivers provide an opportunity to determine Total Electron Content (TEC) over Southern Africa by taking advantage of the dispersive nature of the ionospheric medium. For this task, the University of New Brunswick (UNB) ionospheric modelling technique which applies a spatial linear approximation of the vertical TEC above each station using stochastic parameters in Kalman filter estimation, primed with data from the Southern Africa GPS network, was used for mapping TEC at South African locations during selected days and hours of different epochs of solar cycle 23. Significant enhancements in the TEC value and features, which could be associated with frequent solar events, are evident around a day of extreme solar maximum. These observations are discussed and further investigated by analyzing the GOES 8 and 10 satellites X-ray flux (0.1–0.8 nm) and SOHO Solar EUV Monitor (26.0–34.0 nm) higher resolution data. Comparison of these physical quantities reveals that for each X-ray flare observed, there is an associated EUV flare event. The latter phenomenon causes photoionisation in the daytime ionosphere which results in significant TEC enhancement. The daytime UNB TEC compared with the International Reference Ionosphere (IRI) 2001 predicted TEC found both models to show a good agreement

Extracting Independent Local Oscillatory Geophysical Signals by Geodetic Tropospheric Delay

Zenith Tropospheric Delay (ZTD) due to water vapor derived from space geodetic techniques and numerical weather prediction simulated-reanalysis data exhibits non-linear and non-stationary properties akin to those in the crucial geophysical signals of interest to the research community. These time series, once decomposed into additive (and stochastic) components, have information about the long term global change (the trend) and other interpretable (quasi-) periodic components such as seasonal cycles and noise. Such stochastic component(s) could be a function that exhibits at most one extremum within a data span or a monotonic function within a certain temporal span. In this contribution, we examine the use of the combined Ensemble Empirical Mode Decomposition (EEMD) and Independent Component Analysis (ICA): the EEMD-ICA algorithm to extract the independent local oscillatory stochastic components in the tropospheric delay derived from the European Centre for Medium-Range Weather Forecasts (ECMWF) over six geodetic sites (HartRAO, Hobart26, Wettzell, Gilcreek, Westford, and Tsukub32). The proposed methodology allows independent geophysical processes to be extracted and assessed. Analysis of the quality index of the Independent Components (ICs) derived for each cluster of local oscillatory components (also called the Intrinsic Mode Functions (IMFs)) for all the geodetic stations considered in the study demonstrate that they are strongly site dependent. Such strong dependency seems to suggest that the localized geophysical signals embedded in the ZTD over the geodetic sites are not correlated. Further, from the viewpoint of non-linear dynamical systems, four geophysical signals the Quasi-Biennial Oscillation (QBO) index derived from the NCEP/NCAR reanalysis, the Southern Oscillation Index (SOI) anomaly from NCEP, the SIDC monthly Sun Spot Number (SSN), and the Length of Day (LoD) are linked to the extracted signal components from ZTD. Results from the synchronization analysis show that ZTD and the geophysical signals exhibit (albeit subtle) site dependent phase synchronization index

Revisiting the proximity principle with stakeholder input: Investigating property values and distance to urban green space in potchefstroom

© 2020 by the authors. Nature is essential to urban quality of life, yet green spaces are under pressure. In an attempt to strengthen the case for urban greening and to reclaim nature into cities, this research considered green spaces from an economic spatial perspective. The proximity principle, as part of hedonic price analysis, is employed to determine the impact of green spaces on property value in specifically selected residential areas within Potchefstroom, South Africa. Our statistical analysis indicated a rejection of the proximity principle in some areas, contradicting internationally accepted theory. To investigate local trends and possible reasons for the rejection, supporting quantitative data was gathered through structured questionnaires disseminated to local residents of Potchefstroom and Professional Planners in South Africa. Challenges pertaining to the planning of green spaces were emphasised, despite residents' willingness to pay more for such green spaces in close proximity to residential areas, according to the cross-tabulations conducted. The research results contributed to the discourse on the economic benefits of green spaces and presented the trends of such benefits within the local context of Potchefstroom. The results emphasised the need to rethink the planning of green spaces within the local context, and provided recommendations on how to reclaim nature into cities from a spatial planning perspective

A Variant PfCRT Isoform Can Contribute to Plasmodium falciparum Resistance to the First-Line Partner Drug Piperaquine

Current efforts to reduce the global burden of malaria are threatened by the rapid spread throughout Asia of Plasmodium falciparum resistance to artemisininbased combination therapies, which includes increasing rates of clinical failure with dihydroartemisinin plus piperaquine (PPQ) in Cambodia. Using zinc finger nucleasebased gene editing, we report that addition of the C101F mutation to the chloroquine (CQ) resistance-conferring PfCRT Dd2 isoform common to Asia can confer PPQ resistance to cultured parasites. Resistance was demonstrated as significantly higher PPQ concentrations causing 90% inhibition of parasite growth (IC90) or 50% parasite killing (50% lethal dose [LD50]). This mutation also reversed Dd2-mediated CQ resistance, sensitized parasites to amodiaquine, quinine, and artemisinin, and conferred amantadine and blasticidin resistance. Using heme fractionation assays, we demonstrate that PPQ causes a buildup of reactive free heme and inhibits the formation of chemically inert hemozoin crystals. Our data evoke inhibition of heme detoxification in the parasite’s acidic digestive vacuole as the primary mode of both the bisaminoquinoline PPQ and the related 4-aminoquinoline CQ. Both drugs also inhibit hemoglobin proteolysis at elevated concentrations, suggesting an additional mode of action. Isogenic lines differing in their pfmdr1 copy number showed equivalent PPQ susceptibilities. We propose that mutations in PfCRT could contribute to a multifactorial basis of PPQ resistance in field isolates