987 research outputs found
Registros de representação semiótica nas pesquisas brasileiras em Educação Matemática: pontuando tendências
A questão proposta neste artigo é a de trazer reflexões sobre a forma
de utilização dos estudos de Raymond Duval sobre o papel dos registros de
representação semiótica no ensino e na aprendizagem da matemática, a partir
da análise das pesquisas realizadas no Brasil no perÃodo de 1990 a 2005, que
utilizam os pressupostos teóricos desenvolvidos por esse autor, como principal
fundamento em suas investigações. Este estudo se faz importante por permitir
pontuar a tendência das pesquisas brasileiras nesse foco e traçar o panorama
geral dessas investigações, como forma de apontar possibilidades de utilização
dessa teoria em um âmbito maior: na organização de propostas curriculares e na
formação, tanto inicial quanto continuada de professores que ensinam
matemática
Registros de representação semiótica nas pesquisas brasileiras em educação matemática: pontuando tendências
A questão proposta neste artigo é a de trazer reflexões sobre a forma de utilização dos estudos de Raymond Duval sobre o papel dos registros de representação semiótica no ensino e na aprendizagem da matemática, a partir da análise das pesquisas realizadas no Brasil no perÃodo de 1990 a 2005, que utilizam os pressupostos teóricos desenvolvidos por esse autor, como principal fundamento em suas investigações. Este estudo se faz importante por permitir pontuar a tendência das pesquisas brasileiras nesse foco e traçar o panorama geral dessas investigações, como forma de apontar possibilidades de utilização dessa teoria em um âmbito maior: na organização de propostas curriculares e na formação, tanto inicial quanto continuada de professores que ensinam matemática
Structure of the TPR Domain of AIP: Lack of Client Protein Interaction with the C-Terminal alpha-7 Helix of the TPR Domain of AIP Is Sufficient for Pituitary Adenoma Predisposition
PMCID: PMC3534021This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Oral Microbiome Profiles: 16S rRNA Pyrosequencing and Microarray Assay Comparison
The human oral microbiome is potentially related to diverse health conditions and high-throughput technology provides the possibility of surveying microbial community structure at high resolution. We compared two oral microbiome survey methods: broad-based microbiome identification by 16S rRNA gene sequencing and targeted characterization of microbes by custom DNA microarray.Oral wash samples were collected from 20 individuals at Memorial Sloan-Kettering Cancer Center. 16S rRNA gene survey was performed by 454 pyrosequencing of the V3–V5 region (450 bp). Targeted identification by DNA microarray was carried out with the Human Oral Microbe Identification Microarray (HOMIM). Correlations and relative abundance were compared at phylum and genus level, between 16S rRNA sequence read ratio and HOMIM hybridization intensity.; Correlation = 0.70–0.84).Microbiome community profiles assessed by 16S rRNA pyrosequencing and HOMIM were highly correlated at the phylum level and, when comparing the more commonly detected taxa, also at the genus level. Both methods are currently suitable for high-throughput epidemiologic investigations relating identified and more common oral microbial taxa to disease risk; yet, pyrosequencing may provide a broader spectrum of taxa identification, a distinct sequence-read record, and greater detection sensitivity
Elastically driven, intermittent microscopic dynamics in soft solids
Soft solids with tunable mechanical response are at the core of new material
technologies, but a crucial limit for applications is their progressive aging
over time, which dramatically affects their functionalities. The generally
accepted paradigm is that such aging is gradual and its origin is in slower
than exponential microscopic dynamics, akin to the ones in supercooled liquids
or glasses. Nevertheless, time- and space-resolved measurements have provided
contrasting evidence: dynamics faster than exponential, intermittency, and
abrupt structural changes. Here we use 3D computer simulations of a microscopic
model to reveal that the timescales governing stress relaxation respectively
through thermal fluctuations and elastic recovery are key for the aging
dynamics. When thermal fluctuations are too weak, stress heterogeneities
frozen-in upon solidification can still partially relax through elastically
driven fluctuations. Such fluctuations are intermittent, because of strong
correlations that persist over the timescale of experiments or simulations,
leading to faster than exponential dynamics.Comment: 7 pages, Supplementary Information include
Coxiella burnetii Phagocytosis Is Regulated by GTPases of the Rho Family and the RhoA Effectors mDia1 and ROCK
The GTPases belonging to the Rho family control the actin cytoskeleton rearrangements needed for particle internalization during phagocytosis. ROCK and mDia1 are downstream effectors of RhoA, a GTPase involved in that process. Coxiella burnetii, the etiologic agent of Q fever, is internalized by the host´s cells in an actin-dependent manner. Nevertheless, the molecular mechanism involved in this process has been poorly characterized. This work analyzes the role of different GTPases of the Rho family and some downstream effectors in the internalization of C. burnetii by phagocytic and non-phagocytic cells. The internalization of C. burnetii into HeLa and RAW cells was significantly inhibited when the cells were treated with Clostridium difficile Toxin B which irreversibly inactivates members of the Rho family. In addition, the internalization was reduced in HeLa cells that overexpressed the dominant negative mutants of RhoA, Rac1 or Cdc42 or that were knocked down for the Rho GTPases. The pharmacological inhibition or the knocking down of ROCK diminished bacterium internalization. Moreover, C. burnetii was less efficiently internalized in HeLa cells overexpressing mDia1-N1, a dominant negative mutant of mDia1, while the overexpression of the constitutively active mutant mDia1-ΔN3 increased bacteria uptake. Interestingly, when HeLa and RAW cells were infected, RhoA, Rac1 and mDia1 were recruited to membrane cell fractions. Our results suggest that the GTPases of the Rho family play an important role in C. burnetii phagocytosis in both HeLa and RAW cells. Additionally, we present evidence that ROCK and mDia1, which are downstream effectors of RhoA, are involved in that processFil: Salinas Ojeda, Romina Paola. Consejo Nacional de Investigaciones CientÃficas y Técnicas. Centro CientÃfico Tecnológico Conicet - Mendoza. Instituto de HistologÃa y EmbriologÃa de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de HistologÃa y EmbriologÃa de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Ortiz Flores, Rodolfo Matias. Consejo Nacional de Investigaciones CientÃficas y Técnicas. Centro CientÃfico Tecnológico Conicet - Mendoza. Instituto de HistologÃa y EmbriologÃa de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de HistologÃa y EmbriologÃa de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Distel, Jesús Sebastián. Consejo Nacional de Investigaciones CientÃficas y Técnicas. Centro CientÃfico Tecnológico Conicet - Mendoza. Instituto de HistologÃa y EmbriologÃa de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de HistologÃa y EmbriologÃa de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Aguilera, Milton Osmar. Consejo Nacional de Investigaciones CientÃficas y Técnicas. Centro CientÃfico Tecnológico Conicet - Mendoza. Instituto de HistologÃa y EmbriologÃa de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de HistologÃa y EmbriologÃa de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Colombo, Maria Isabel. Consejo Nacional de Investigaciones CientÃficas y Técnicas. Centro CientÃfico Tecnológico Conicet - Mendoza. Instituto de HistologÃa y EmbriologÃa de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de HistologÃa y EmbriologÃa de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Beron, Walter. Consejo Nacional de Investigaciones CientÃficas y Técnicas. Centro CientÃfico Tecnológico Conicet - Mendoza. Instituto de HistologÃa y EmbriologÃa de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de HistologÃa y EmbriologÃa de Mendoza Dr. Mario H. Burgos; Argentin
Consumer perceptions of co-branding alliances: Organizational dissimilarity signals and brand fit
This study explores how consumers evaluate co-branding alliances between dissimilar partner firms. Customers are well aware that different firms are behind a co-branded product and observe the partner firms’ characteristics. Drawing on signaling theory, we assert that consumers use organizational characteristics as signals in their assessment of brand fit and for their purchasing decisions. Some organizational signals are beyond the control of the co-branding partners or at least they cannot alter them on short notice. We use a quasi-experimental design and test how co-branding partner dissimilarity affects brand fit perception. The results show that co-branding partner dissimilarity in terms of firm size, industry scope, and country-of-origin image negatively affects brand fit perception. Firm age dissimilarity does not exert significant influence. Because brand fit generally fosters a benevolent consumer attitude towards a co-branding alliance, the findings suggest that high partner dissimilarity may reduce overall co-branding alliance performance
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