47 research outputs found
In vitro neuronal cultures on MEA: an engineering approach to study physiological and pathological brain networks
Reti neuronali accoppiate a matrici di microelettrodi: un metodo ingegneristico per studiare reti cerebrali in situazioni fisiologiche e patologich
A Neuromorphic Prosthesis to Restore Communication in Neuronal Networks
Recent advances in bioelectronics and neural engineering allowed the development of brain machine interfaces and neuroprostheses, capable of facilitating or recovering functionality in people with neurological disability. To realize energy-efficient and real-time capable devices, neuromorphic computing systems are envisaged as the core of next-generation systems for brain repair. We demonstrate here a real-time hardware neuromorphic prosthesis to restore bidirectional interactions between two neuronal populations, even when one is damaged or missing. We used in vitro modular cell cultures to mimic the mutual interaction between neuronal assemblies and created a focal lesion to functionally disconnect the two populations. Then, we employed our neuromorphic prosthesis for bidirectional bridging to artificially reconnect two disconnected neuronal modules and for hybrid bidirectional bridging to replace the activity of one module with a real-time hardware neuromorphic Spiking Neural Network. Our neuroprosthetic system opens avenues for the exploitation of neuromorphic-based devices in bioelectrical therapeutics for health care
Phosphocaveolin-1 enforces tumor growth and chemoresistance in rhabdomyosarcoma
Caveolin-1 (Cav-1) can ambiguously behave as either tumor suppressor or oncogene depending on its phosphorylation state and the type of cancer. In this study we show that Cav-1 was phosphorylated on tyrosine 14 (pCav-1) by Src-kinase family members in various human cell lines and primary mouse cultures of rhabdomyosarcoma (RMS), the most frequent soft-tissue sarcoma affecting childhood. Cav-1 overexpression in the human embryonal RD or alveolar RH30 cells yielded increased pCav-1 levels and reinforced the phosphorylation state of either ERK or AKT kinase, respectively, in turn enhancing in vitro cell proliferation, migration, invasiveness and chemoresistance. In contrast, reducing the pCav-1 levels by administration of a Src-kinase inhibitor or through targeted Cav-1 silencing counteracted the malignant in vitro phenotype of RMS cells. Consistent with these results, xenotransplantation of Cav-1 overexpressing RD cells into nude mice resulted in substantial tumor growth in comparison to control cells. Taken together, these data point to pCav-1 as an important and therapeutically valuable target for overcoming the progression and multidrug resistance of RMS
Disentangling the initiation from the response in joint attention: An eye-tracking study in toddlers with autism spectrum disorders
Joint attention (JA), whose deficit is an early risk marker for autism spectrum disorder (ASD), has two dimensions: (1) responding to JA and (2) initiating JA. Eye-tracking technology has largely been used to investigate responding JA, but rarely to study initiating JA especially in young children with ASD. The aim of this study was to describe the differences in the visual patterns of toddlers with ASD and those with typical development (TD) during both responding JA and initiating JA tasks. Eye-tracking technology was used to monitor the gaze of 17 children with ASD and 15 age-matched children with TD during the presentation of short video sequences involving one responding JA and two initiating JA tasks (initiating JA-1 and initiating JA-2). Gaze accuracy, transitions and fixations were analyzed. No differences were found in the responding JA task between children with ASD and those with TD, whereas, in the initiating JA tasks, different patterns of fixation and transitions were shown between the groups. These results suggest that children with ASD and those with TD show different visual patterns when they are expected to initiate joint attention but not when they respond to joint attention. We hypothesized that differences in transitions and fixations are linked to ASD impairments in visual disengagement from face, in global scanning of the scene and in the ability to anticipate object's action
Lifestyles and socio-cultural factors among children aged 6-8 years from five Italian towns: The MAPEC-LIFE study cohort
Background: Lifestyles profoundly determine the quality of an individual’s health and life since his childhood.
Many diseases in adulthood are avoidable if health-risk behaviors are identified and improved at an early stage of
life. The aim of the present research was to characterize a cohort of children aged 6–8 years selected in order to
perform an epidemiological molecular study (the MAPEC_LIFE study), investigate lifestyles of the children that
could have effect on their health status, and assess possible association between lifestyles and socio-cultural factors.
Methods: A questionnaire composed of 148 questions was administered in two different seasons to parents of
children attending 18 primary schools in five Italian cities (Torino, Brescia, Pisa, Perugia and Lecce) to obtain
information regarding the criteria for exclusion from the study, demographic, anthropometric and health
information on the children, as well as some aspects on their lifestyles and parental characteristics. The results
were analyzed in order to assess the frequency of specific conditions among the different seasons and cities and
the association between lifestyles and socio-economic factors.
Results: The final cohort was composed of 1,164 children (50.9 boys, 95.4% born in Italy). Frequency of some
factors appeared different in terms of the survey season (physical activity in the open air, the ways of cooking
certain foods) and among the various cities (parents’ level of education and rate of employment, sport, traffic
near the home, type of heating, exposure to passive smoking, ways of cooking certain foods). Exposure to
passive smoking and cooking fumes, obesity, residence in areas with heavy traffic, frequency of outdoor play and
consumption of barbecued and fried foods were higher among children living in families with low educational
and/or occupational level while children doing sports and consuming toasted bread were more frequent in families
with high socio-economic level.
Conclusions: The socio-economic level seems to affect the lifestyles of children enrolled in the study including
those that could cause health effects. Many factors are linked to the geographical area and may depend on
environmental, cultural and social aspects of the city of residence
Computed tomography-histologic correlations in lung cancer
The multidisciplinary approach is ideal in the management of patients with lung cancer. Multidisciplinary evaluation strengthens the differential diagnosis of aspecific radiological findings, indeed. Notably, the differential diagnosis of early stage lung cancer is a current challenge of CT imaging because the earlier the detection, the lower the accuracy of radiological features. This is particularly true for the most common subtype of lung cancer, adenocarcinoma, because it shows various radiological features. Such variety is also reflected by the 2011 classification of lung cancer, that likely affected the diagnostic agreement between radiologist and clinician. This review discusses the common issues of lung cancer diagnosis by paired radiological-histologic interpretation of CT findings
Real-time detection of bursts in neuronal cultures using a Neuromorphic Auditory Sensor and Spiking Neural Networks
The correct identi cation of burst events is crucial in many scenarios,
ranging from basic neuroscience to biomedical applications. However, none
of the burst detection methods that can be found in the literature have been
widely adopted for this task. As an alternative to conventional techniques, a
novel neuromorphic approach for real-time burst detection is proposed and
tested on acquisitions from in vitro cultures. The system consists of a Neuromorphic
Auditory Sensor, which converts the input signal obtained from
electrophysiological recordings into spikes and decomposes them into di erent
frequency bands. The output of the sensor is sent to a trained spiking
neural network implemented on a SpiNNaker board that discerns between
bursting and non-bursting activity. This data-driven approach was compared
with 8 di erent conventional spike-based methods, addressing some of
their drawbacks, such as being able to detect both high and low frequency
events and working in an online manner. Similar results in terms of number
of detected events, mean burst duration and correlation as current state-ofthe-
art approaches were obtained with the proposed system, also bene ting from its lower power consumption and computational latency. Therefore,
our neuromorphic-based burst detection paves the road to future implementations
for neuroprosthetic applications.Spanish Ministry of Education, Culture and Sport "Formación de Personal Universitario Scholarship"European Regional Development Fund COFNET TEC2016-77785-