Home for Good? Preparing to Support People with Learning Difficulties in Residential Settings when they Develop Dementia

This report explores the findings of a study which investigated the current models of practice for supporting people with learning difficulties and dementia living in care home settings. It looked at the key issues and discovered examples of best practice in relating to providing care homes for this group. The report includes a poster with some quick tips for staff supporting people with learning difficulties and dementia

Exposure to bile influences biofilm formation by Listeria monocytogenes

In the present study we demonstrate that the initial attachment of Listeria monocytogenes cells to plastic surfaces was significantly increased by growth in the presence of bile. Improved biofilm formation was confirmed by crystal violet staining, microscopy and bioluminescence detection of a luciferase-tagged strain. Enhanced biofilm formation in response to bile may influence the ability of L. monocytogenes to form biofilms in vivo during infection and may contribute to survival of this important pathogen in the human gastrointestinal tract and gallbladder

Clinically guided core biopsy and cutaneous punch biopsy in the evaluation of breast lesions:a necessary test or an obsolete skill?

OBJECTIVE: The vast majority of breast cancers are diagnosed via image-guided procedures yet despite significant advances, imaging does not identify all breast malignancies. Clinically suspicious breast lesions with normal breast imaging remain a cause for concern. The aim of this study is to determine the diagnostic value of clinical core and cutaneous punch biopsies in the diagnosis of breast malignancy in clinically suspicious lesions with normal breast imaging. METHODS: All patients with suspicious clinical breast findings and normal imaging who underwent a clinical core and/or cutaneous punch biopsy from 2012 to 2019 were reviewed retrospectively. Patients with subsequent breast malignant diagnosis were analysed. RESULTS: A total of 283 biopsies (166 clinical core, 117 cutaneous punch) performed over the 7-year period were included in the analysis. A total of 263/283 (93%) yielded a benign outcome. A total of 2/283 (0.7%) yielded B3 lesions (probably benign). These lesions were benign on final surgical excision. A total of 18/283 (6.3%) yielded a malignant histopathology. Sixteen out of 18 were cutaneous punch biopsies, and 2/18 were clinical core biopsies. A total of 14/18 patients presented with nipple changes, while 4/18 had a palpable area of concern. Histopathological analysis demonstrated Paget’s disease of the nipple in 8/18, invasive carcinoma in 9/18 out of which two represented a recurrence of breast malignancy. Cutaneous squamous cell carcinoma was diagnosed in 1/18. CONCLUSION: Clinical core and cutaneous punch biopsies remain a valuable tool in the diagnosis of breast cancer particularly in the management of clinically suspicious radiographically occult malignancies

African Drought Risk Pay-Out Benchmarking

This report contains exploratory data analysis of rainfall and Water Resource Sufficiency Index (WRSI) data provided by African Risk Ca- pacity (ARC). The purpose is to assess the predictability of droughts in Africa. We assess the appropriateness of the historical WRSI bench- marks set by ARC members compared to the observed WRSI values for different regions. We conclude that the benchmarks are broadly sensible. We then compare a number of linear time series models based on their ability to fit and forecast the WRSI time series. We conclude that sim- pler models like Simple Moving Average and Moving Median are more appropriate than more sophisticated models containing trends and sea- sonality like Holt Winter and TBATS. We also investigate the use of the SARIMA and TBATS models to forecast the seasonal patterns observed in rainfall data and conclude that both models can generate structured forecasts that reflect seasonal variability. The statistical evidence how- ever favoured TBATS over SARIMA. Attempts to measure the influence of the El Nin ̃o-Southern Oscillation on rainfall levels are inconclusive for the areas studied. Finally we perform a simple application of univariate Extreme Value Theory to rainfall data and conclude that further inves- tigation is necessary to understand how the catastrophic famine that affected Ethiopia in the early 1980’s would be reflected in the data if a similar event were to reoccur today

Improved diagnosis of SARS-CoV-2 by using nucleoprotein and spike protein fragment 2 in quantitative dual ELISA tests

The novel coronavirus, severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2), is the causative agent of the 2020 worldwide coronavirus pandemic. Antibody testing is useful for diagnosing historic infections of a disease in a population. These tests are also a helpful epidemiological tool for predicting how the virus spreads in a community, relating antibody levels to immunity and for assessing herd immunity. In the present study, SARS-CoV-2 viral proteins were recombinantly produced and used to analyse serum from individuals previously exposed, or not, to SARS-CoV-2. The nucleocapsid (Npro) and spike subunit 2 (S2Frag) proteins were identified as highly immunogenic, although responses to the former were generally greater. These two proteins were used to develop two quantitative enzyme-linked immunosorbent assays (ELISAs) that when used in combination resulted in a highly reliable diagnostic test. Npro and S2Frag-ELISAs could detect at least 10% more true positive coronavirus disease-2019 (COVID-19) cases than the commercially available ARCHITECT test (Abbott). Moreover, our quantitative ELISAs also show that specific antibodies to SARS-CoV-2 proteins tend to wane rapidly even in patients who had developed severe disease. As antibody tests complement COVID-19 diagnosis and determine population-level surveillance during this pandemic, the alternative diagnostic we present in this study could play a role in controlling the spread of the virus

Diversity of Bifidobacteria within the Infant Gut Microbiota

Background The human gastrointestinal tract (GIT) represents one of the most densely populated microbial ecosystems studied to date. Although this microbial consortium has been recognized to have a crucial impact on human health, its precise composition is still subject to intense investigation. Among the GIT microbiota, bifidobacteria represent an important commensal group, being among the first microbial colonizers of the gut. However, the prevalence and diversity of members of the genus Bifidobacterium in the infant intestinal microbiota has not yet been fully characterized, while some inconsistencies exist in literature regarding the abundance of this genus. Methods/Principal Findings In the current report, we assessed the complexity of the infant intestinal bifidobacterial population by analysis of pyrosequencing data of PCR amplicons derived from two hypervariable regions of the 16 S rRNA gene. Eleven faecal samples were collected from healthy infants of different geographical origins (Italy, Spain or Ireland), feeding type (breast milk or formula) and mode of delivery (vaginal or caesarean delivery), while in four cases, faecal samples of corresponding mothers were also analyzed. Conclusions In contrast to several previously published culture-independent studies, our analysis revealed a predominance of bifidobacteria in the infant gut as well as a profile of co-occurrence of bifidobacterial species in the infant’s intestine

Eo-miners: monitoring the environmental and societal impact of the extractive industry using earth observation

The growing demand for mineral and energy resources over the last decade has placed the extractive industry under increasing pressure to monitor and reduce the environmental and societal impact throughout the life-cycle of mining operations. Despite the mounting pressure, the industry is still facing the challenge of how to define targets for, and monitor, the impact of mining. In 2010, the EU-funded EO-MINERS project (Earth Observation for Monitoring and Observing Environmental and Societal Impacts of Mineral Resources Exploration and Exploitation) was set up in an effort to help address this issue, specifically through the application of Earth Observation (EO) data. Furthermore, the aim was to help facilitate and improve interaction and dialogue between the mineral extractive industry and society in view of its sustainable development, while improving its societal acceptability. One of the primary project objectives was to develop novel yet objective EO products contributing to a constructive “trialogue” involving stakeholders such as industrialists (mining companies), regulatory bodies and the civil society. EO-MINERS is scheduled to run until October 2013