Automation of a Positron-emission Tomography (PET) Radiotracer Synthesis Protocol for Clinical Production.

The development of new positron-emission tomography (PET) tracers is enabling researchers and clinicians to image an increasingly wide array of biological targets and processes. However, the increasing number of different tracers creates challenges for their production at radiopharmacies. While historically it has been practical to dedicate a custom-configured radiosynthesizer and hot cell for the repeated production of each individual tracer, it is becoming necessary to change this workflow. Recent commercial radiosynthesizers based on disposable cassettes/kits for each tracer simplify the production of multiple tracers with one set of equipment by eliminating the need for custom tracer-specific modifications. Furthermore, some of these radiosynthesizers enable the operator to develop and optimize their own synthesis protocols in addition to purchasing commercially-available kits. In this protocol, we describe the general procedure for how the manual synthesis of a new PET tracer can be automated on one of these radiosynthesizers and validated for the production of clinical-grade tracers. As an example, we use the ELIXYS radiosynthesizer, a flexible cassette-based radiochemistry tool that can support both PET tracer development efforts, as well as routine clinical probe manufacturing on the same system, to produce [18F]Clofarabine ([18F]CFA), a PET tracer to measure in vivo deoxycytidine kinase (dCK) enzyme activity. Translating a manual synthesis involves breaking down the synthetic protocol into basic radiochemistry processes that are then translated into intuitive chemistry "unit operations" supported by the synthesizer software. These operations can then rapidly be converted into an automated synthesis program by assembling them using the drag-and-drop interface. After basic testing, the synthesis and purification procedure may require optimization to achieve the desired yield and purity. Once the desired performance is achieved, a validation of the synthesis is carried out to determine its suitability for the production of the radiotracer for clinical use

Tool-free, Cosmetically Hidden Phone Display and Back Cover Disassembly Port

The back cover and/or display displays of many portable devices such as smartphones and tablets are often integrated with the main assembly. If the back cover assembly or the phone display needs to be removed (e.g., to access the battery) or needs to be replaced, the device owner needs to approach a service center for such work since specialized tools are often required for removal. This disclosure describes a mechanism for easy removal of back cover or display of a device without impacting aesthetics of the device. Per the techniques of this disclosure, a slot is machined or molded on the adhesive landing between the display/ back cover and the SIM tray opening. The slot can be accessed easily with the SIM tray tool or a paperclip

Geotechnical Observations of the November 3, 2002 M7.9 Denali Fault Earthquake

The M 7.9 earthquake of November 3, 2002 event ruptured more than 340 kilometers on three fault, causing widespread liquefaction in the fluvial deposits of steep alpine valleys of the Alaska Range and eastern lowlands of the Tanana River. The event occurred in a remote and largely undeveloped portion of the rugged Alaskan central range, with few seismometer recordings. The areas affected by liquefaction are largely confined to native Holocene river deposits, areas bounded by stiffer ground moraine, Pleistocene uplands, and bedrock. Liquefaction affected areas of alluvial river valleys draining mountainous and glacier-proximal rivers. The most noteworthy observations are that liquefaction damage was focused towards the eastern end of the rupture area. In the western portion of the rupture zone, localized liquefaction developed in recent deposits of the Susitna and Delta rivers in the immediate vicinity of the surface rupture of the fault. More abundant and severe liquefaction occurred on the eastern Robertson, Slana, Tok, Chisana and, especially, Nabesna Rivers. In the Tanana lowland, liquefaction features were sparse on the western bars of the Tanana River in the vicinity of Fairbanks to west of Delta, but became pervasive throughout the eastern region from Delta to Northway. Though liquefaction observations were abundant, there was a dearth of instrumental recordings useful to relate damage effects to measured intensity. To characterize soil properties and stiffness of liquefaction evaluation sites, we used a portable spectral analysis of surface waves (SASW) apparatus to profile the shear wave velocity of the ground. On the Nabesna and Delta rivers that cross the fault, we only observe liquefaction features in soil deposits where normalized shear wave velocities fall below 230 m/s. Severity of sand boils, fissuring and lateral displacement of liquefied ground dramatically increase in soils of lower shear wave velocity, especially below 170 m/s. Some of the most pronounced ground failures are far from the fault zone (60-100 km) in extremely loose, low velocity (~120 m/s) fine sands of the bars of the Tanana River. Strong motion instrumentation was sparse within 150 kilometers of the fault rupture and the seismometers of Alyeska pump stations PS9 (PGA=0.09), PS10 (PGA=0.36g), and PS11 (PGA=0.09) serve as the principal strong motion recordings. Insufficient strong motion instrumentation is available to identify areas of amplified ground motio

Gluon Fragmentation into Heavy Quarkonium

The dominant production mechanism for heavy quark-antiquark bound states in very high energy processes is fragmentation, the splitting of a high energy parton into a quarkonium state and other partons. We show that the fragmentation functions $D(z,\mu)$ describing these processes can be calculated using perturbative QCD. We calculate the fragmentation functions for a gluon to split into S-wave quarkonium states to leading order in the QCD coupling constant. The leading logarithms of $\mu/m_Q$, where $\mu$ is the factorization scale and $m_Q$ is the heavy quark mass, are summed up using Altarelli-Parisi evolution equations.Comment: LateX 11 pages (3 figures available upon request). NUHEP-TH-92-2

A mitochondrial-focused genetic interaction map reveals a scaffold-like complex required for inner membrane organization in mitochondria.

To broadly explore mitochondrial structure and function as well as the communication of mitochondria with other cellular pathways, we constructed a quantitative, high-density genetic interaction map (the MITO-MAP) in Saccharomyces cerevisiae. The MITO-MAP provides a comprehensive view of mitochondrial function including insights into the activity of uncharacterized mitochondrial proteins and the functional connection between mitochondria and the ER. The MITO-MAP also reveals a large inner membrane-associated complex, which we term MitOS for mitochondrial organizing structure, comprised of Fcj1/Mitofilin, a conserved inner membrane protein, and five additional components. MitOS physically and functionally interacts with both outer and inner membrane components and localizes to extended structures that wrap around the inner membrane. We show that MitOS acts in concert with ATP synthase dimers to organize the inner membrane and promote normal mitochondrial morphology. We propose that MitOS acts as a conserved mitochondrial skeletal structure that differentiates regions of the inner membrane to establish the normal internal architecture of mitochondria

Exponentiation of the Drell-Yan cross section near partonic threshold in the DIS and MSbar schemes

It has been observed that in the DIS scheme the refactorization of the Drell-Yan cross section leading to exponentiation of threshold logarithms can also be used to organize a class of constant terms, most of which arise from the ratio of the timelike Sudakov form factor to its spacelike counterpart. We extend this exponentiation to include all constant terms, and demonstrate how a similar organization may be achieved in the MSbar scheme. We study the relevance of these exponentiations in a two-loop analysis.Comment: 20 pages, JHEP style, no figure