T-cell derived acetylcholine aids host defenses during enteric bacterial infection with Citrobacter rodentium.

The regulation of mucosal immune function is critical to host protection from enteric pathogens but is incompletely understood. The nervous system and the neurotransmitter acetylcholine play an integral part in host defense against enteric bacterial pathogens. Here we report that acetylcholine producing-T-cells, as a non-neuronal source of ACh, were recruited to the colon during infection with the mouse pathogen Citrobacter rodentium. These ChAT+ T-cells did not exclusively belong to one Th subset and were able to produce IFNγ, IL-17A and IL-22. To interrogate the possible protective effect of acetylcholine released from these cells during enteric infection, T-cells were rendered deficient in their ability to produce acetylcholine through a conditional gene knockout approach. Significantly increased C. rodentium burden was observed in the colon from conditional KO (cKO) compared to WT mice at 10 days post-infection. This increased bacterial burden in cKO mice was associated with increased expression of the cytokines IL-1β, IL-6, and TNFα, but without significant changes in T-cell and ILC associated IL-17A, IL-22, and IFNγ, or epithelial expression of antimicrobial peptides, compared to WT mice. Despite the increased expression of pro-inflammatory cytokines during C. rodentium infection, inducible nitric oxide synthase (Nos2) expression was significantly reduced in intestinal epithelial cells of ChAT T-cell cKO mice 10 days post-infection. Additionally, a cholinergic agonist enhanced IFNγ-induced Nos2 expression in intestinal epithelial cell in vitro. These findings demonstrated that acetylcholine, produced by specialized T-cells that are recruited during C. rodentium infection, are a key mediator in host-microbe interactions and mucosal defenses

Design and implementation of a strategic process for an integrated management of the hydrologic resources of the Lerma-Chapala-Santiago watershed

La Red-Lerma tiene por objetivo facilitar y contribuir al fortalecimiento de las capacidades de la cuenca Lerma-Chapala-Santiago, a través de la generación conjunta de conocimientos, la formación de especialistas y la elaboración de alternativas a los problemas que los recursos hídricos enfrentan en la zona, permitiendo mejorar la calidad de vida de sus habitantes y su ambiente. En este contexto, la Red Lerma desarrolló una guía estructurada en torno a la Gestión Integrada de Recursos Hídricos (GIRH), con base en un proceso de planeación estratégica participativa, usando los indicadores Presión-Estado-Impacto-Respuesta y bajo un esquema de trabajo en Red, útil para los tomadores de decisiones de gobierno (Federal, Estatal y Municipal), organizaciones e instituciones, que convergen en in espacio inter-institucional e inter-disciplinario de una red local (Unidad Interdisciplinaria de Trabajo-UIT). En el ámbito regional, se han iniciado los procesos de integración de las UIT's en los estado de México, Nayarit, Guanajuato y Michoacán. Se participa además en el desarrollo y seguimiento del Plan Estratégico para la Recuperación de la Cuenca Alta del Río Lerma (CARL), así como en los trabajos de la Mesa Técnica de Educación Ambiental y Participación Social, ambos impulsados por la Universidad Autónoma del Estado de México y el Gobierno del Estado de México (GEM)

Erratum: Author Correction: Identification of genes required for eye development by high-throughput screening of mouse knockouts.

[This corrects the article DOI: 10.1038/s42003-018-0226-0.]

Desafíos de las metrópolis: Efectos ambientales y sociales. Tendencias geográficas II

El libro está conformado de estudios realizados por profesores-investigadores de la Universidad Autónoma del Estado de México, de la Universidad de Varsovia, así como de la Universidad Pedagógica Comisión de Educación Nacional de Cracovia. En esta obra se exponen algunas investigaciones sobre los cambios en los factores sociales, naturales, económicos y ambientales como principales desafios que presentan las zonas de México, Polonia y de contextos de Sudamérica, tales como Sao Paulo, Quito y Bogotá y ciudades medias y pequeñas.Universidad Autónoma del Estado de Méxic

A Data Mining Perspective of XRF Elemental Analysis from Pueblo People’s Pottery

Hierarchical clustering was used to identify elemental signatures in artifacts attributed to the Pueblo peoples. The artifacts in this study are pottery samples found at different sites in the state of New Mexico, USA. Three methods were applied: complete, average, and Ward. Their corresponding cophenetic correlation coefficients were used to contrast the three methods. Elemental characterization was only based on X-ray fluorescence excitation from a portable spectrometer with the silver anode. The elemental correlations here disclosed by data mining techniques are expected to guide further archaeological studies and assist experts in the assessment of provenance and historical ethnographic studies. XRF elemental analysi

Comparison of Dynamics Stability Testing Techniques with Magnetic Suspension Wind Tunnel Capabilities

Dynamic stability testing techniques currently utilized at NASA Langley Research Center (LaRC) are conducted in multiple facilities and consists of free flight, forced oscillation, and free-to-oscillate tests. The NASA/ODU Magnetic Suspension and Balance System (MSBS) has been recommissioned to explore its utility as an additional facility to expand the dynamic stability test capabilities currently available at NASA LaRC. Simulations were created to replicate each current test facility and method as closely as possible. Data collected from the simulated environments was corrupted with replicated noise sources of the different testing environments and then compared to real data collected during tests when such data was available. The corrupted data was then passed through data reduction and System Identification (SID) to estimate the accuracy of the results with the known aerodynamic model that was utilized within the simulation to generate the original data. Magnitudes of noise were varied utilizing Monte Carlo analysis to perform sensitivity analysis of each noise source on the extracted dynamic stability coefficients. Some preliminary results will be presented

Familie und Schule: zwei Orte der Erziehung.

B

El reencuentro con la naturaleza: voces femeninas en el tiempo

Este texto contribuye al análisis científico de diferentes áreas del conocimiento la filosofía social, la educación para el cuidado del medio ambiente mediante la sustentabilidad que incide en diversas unidades de aprendizaje en Educación para la Salud y de la Maestria en Sociología de la SaludEl relato de las voces femeninas que se escuchan en el tiempo, narran las luchas por defender un ideal, con frecuencia en la organización de las mujeres que pugnan por estabilizar el equilibrio de la naturaleza, por medio de tácticas educativas que van recorriendo el bachillerato, la normal y la universidad