Microsleep Episodes, Attention Lapses and Circadian Variation in Psychomotor Performance in a Driving Simulation Paradigm

Numerous studies document circadian changes in sleepiness, with biphasic peaks in the early morning and late afternoon. Driving performance has also been demonstrated to be subject to time-of-day variation. This study investigated circadian variation in driving performance, attention lapses (AL) and/or frequency of microsleep (MS) episodes across the day. Sixteen healthy adults with valid driver’s licenses participated in the study. Using the York Driving Simulator, subjects performed four intentionally soporific 30-minute driving simulations at two-hour intervals (i.e., at 10:00, 12:00, 14:00, and 16:00). During each session, individuals had EEG monitoring for MS episodes (defined as 15 to 30 seconds of any sleep stage by polysomnographic criteria) and AL episodes (defined as intrusion of alpha- or theta-EEG activity lasting 4-14 seconds). Measured variables included: lane accuracy, average speed, speed deviation, mean reaction time (RT) to “virtual” wind gusts and off-road events. Mean values of each variable at every time were analyzed using a general linear model and paired sample t-tests. RT displayed significant within-group variation, with paired samples tests at df=15 showing RT at 10:00 significantly faster than at other times of the day, but no significant within-group variation between other times of the day. All other variables and EEG-defined AL episodes failed to exhibit any statistically significant variation across the day. However, MS episodes were found to occur more often at 16:00 in comparison to all other times. As RT was optimal before noon, it appears that psychomotor performance and therefore driving ability is subject to circadian variation. Coincident with the demonstrated circadian pattern of diminished alertness, this may partially explain the high incidence of motor vehicle accidents during the mid- to late-afternoon. By better understanding circadian fluctuations in driver sleepiness and psychomotor performance, human performance researchers may be in a position to better educate the public about cautionary measures to prevent accidents

A novel NGS library preparation method to characterize native termini of fragmented DNA.

Biological and chemical DNA fragmentation generates DNA molecules with a variety of termini, including blunt ends and single-stranded overhangs. We have developed a Next Generation Sequencing (NGS) assay, XACTLY, to interrogate the termini of fragmented DNA, information traditionally lost in standard NGS library preparation methods. Here we describe the XACTLY method, showcase its sensitivity and specificity, and demonstrate its utility in in vitro experiments. The XACTLY assay is able to report relative abundances of all lengths and types (5' and 3') of single-stranded overhangs, if present, on each DNA fragment with an overall accuracy between 80-90%. In addition, XACTLY retains the sequence of each native DNA molecule after fragmentation and can capture the genomic landscape of cleavage events at single nucleotide resolution. The XACTLY assay can be applied as a novel research and discovery tool for fragmentation analyses and in cell-free DNA

Simulator Performance vs. Neurophysiologic Monitoring: Which is More Relevant to Assess Driving Impairment?

Previously, we reported on circadian variation in driving simulator performance and neurophysiologic evidence of sleep intrusion into consciousness in a pilot study of healthy individuals. We have since expanded this “normative” sample and run a prospective comparison study with a sample of clinical patients reporting excessive daytime sleepiness (EDS) as a chief complaint. Thirty healthy adults (mean age of 31.3 ± 11.5) and 27 EDS patients (47.0 ± 13.7) with valid driver’s licenses were included. Subjects performed four intentionally soporific 30-minute driving simulations at two-hour intervals while undergoing continuous EEG monitoring for microsleep (MS) episodes. Measured variables included: subjective ratings of sleepiness and alertness prior to each drive, lane position accuracy, mean speed, speed deviation, mean reaction time (RT) to “virtual” wind gusts as well as off-road events, i.e., “crashes.” In comparing normative individuals and EDS patients, significant between-group differences were found between subjective ratings, RT, crashes and MS. Both groups showed a significant a tendency towards RT slowing during afternoon drives, with this circadian effect appearing most pronounced for EDS patients. Significant between-group differences were also found on subjective ratings of sleepiness and alertness, although diurnal fluctuation of subjective sleepiness ratings was significant only for the EDS group. Objective EEG MS monitoring demonstrated escalating sleep intrusion with repeated drives in both groups, but particularly for the EDS group. Total crash rates were three times higher in EDS patients, with an increasing trend towards crash-proneness in the late afternoon. In summary, we found significantly impaired performance on some, though not all, driving parameters for EDS patients. While increased crash rate may be the most dramatic of these, slowing of RT was the most statistically robust. EEG monitoring was able to document increased propensity towards MS episodes in patients with EDS, which we suggest is causative in creating this impairment. It remains unclear whether a neurophysiologic or simulator approach captures impairment due to sleepiness with greater sensitivity and specificity. A hybrid approach combining data from both sources may be optimal, and also could be integrated in commercial vehicle use. We suggest that the need for a more accurate hospitalbased screening tool for assessment of driving impairment due to sleep disorders remains an important issue for physicians and legislators dealing with driving competency

Draft genome sequences of gammaproteobacterial methanotrophs isolated from marine ecosystems

The genome sequences of Methylobacter marinus A45, Methylobacter sp. strain BBA5.1, and Methylomarinum vadi IT-4 were obtained. These aerobic methanotrophs are typical members of coastal and hydrothermal vent marine ecosystems

Chaos and Quantum Thermalization

We show that a bounded, isolated quantum system of many particles in a specific initial state will approach thermal equilibrium if the energy eigenfunctions which are superposed to form that state obey {\it Berry's conjecture}. Berry's conjecture is expected to hold only if the corresponding classical system is chaotic, and essentially states that the energy eigenfunctions behave as if they were gaussian random variables. We review the existing evidence, and show that previously neglected effects substantially strengthen the case for Berry's conjecture. We study a rarefied hard-sphere gas as an explicit example of a many-body system which is known to be classically chaotic, and show that an energy eigenstate which obeys Berry's conjecture predicts a Maxwell--Boltzmann, Bose--Einstein, or Fermi--Dirac distribution for the momentum of each constituent particle, depending on whether the wave functions are taken to be nonsymmetric, completely symmetric, or completely antisymmetric functions of the positions of the particles. We call this phenomenon {\it eigenstate thermalization}. We show that a generic initial state will approach thermal equilibrium at least as fast as $O(\hbar/\Delta)t^{-1}$, where $\Delta$ is the uncertainty in the total energy of the gas. This result holds for an individual initial state; in contrast to the classical theory, no averaging over an ensemble of initial states is needed. We argue that these results constitute a new foundation for quantum statistical mechanics.Comment: 28 pages in Plain TeX plus 2 uuencoded PS figures (included); minor corrections only, this version will be published in Phys. Rev. E; UCSB-TH-94-1

Correlative analysis of structure and chemistry of LixFePO4 platelets using 4D-STEM and X-ray ptychography

Lithium iron phosphate (LixFePO4), a cathode material used in rechargeable Li-ion batteries, phase separates upon de/lithiation under equilibrium. The interfacial structure and chemistry within these cathode materials affects Li-ion transport, and therefore battery performance. Correlative imaging of LixFePO4 was performed using four-dimensional scanning transmission electron microscopy (4D-STEM), scanning transmission X-ray microscopy (STXM), and X-ray ptychography in order to analyze the local structure and chemistry of the same particle set. Over 50,000 diffraction patterns from 10 particles provided measurements of both structure and chemistry at a nanoscale spatial resolution (16.6-49.5 nm) over wide (several micron) fields-of-view with statistical robustness.LixFePO4 particles at varying stages of delithiation were measured to examine the evolution of structure and chemistry as a function of delithiation. In lithiated and delithiated particles, local variations were observed in the degree of lithiation even while local lattice structures remained comparatively constant, and calculation of linear coefficients of chemical expansion suggest pinning of the lattice structures in these populations. Partially delithiated particles displayed broadly core-shell-like structures, however, with highly variable behavior both locally and per individual particle that exhibited distinctive intermediate regions at the interface between phases, and pockets within the lithiated core that correspond to FePO4 in structure and chemistry.The results provide insight into the LixFePO4 system, subtleties in the scope and applicability of Vegards law (linear lattice parameter-composition behavior) under local versus global measurements, and demonstrate a powerful new combination of experimental and analytical modalities for bridging the crucial gap between local and statistical characterization.Comment: 17 pages, 4 figure

Density profiles of dark matter haloes: diversity and dependence on environment

(Abridged) We study the outer density profiles of dark matter haloes predicted by a generalized secondary infall model and observed in a N-body cosmological simulation of a \Lambda CDM model. We find substantial systematic variations in shapes and concentrations of the halo profiles as well as a strong correlation of the profiles with the environment. In the N-body simulation, the average outer slope of the density profiles, \beta (\rho\propto r^{-\beta}), of isolated haloes is \approx 2.9; 68% of these haloes have values of \beta between 2.5 and 3.8. Haloes in dense environments of clusters are more concentrated and exhibit a broad distribution of \beta with values larger than for isolated haloes . Contrary to what one may expect, the haloes contained within groups and galaxy systems are less concentrated and have flatter outer density profiles than the isolated haloes. The concentration decreases with M_h, but its scatter for a given mass is substantial. The mass and circular velocity of the haloes are strongly correlated: M_h \propto V_m^{\alpha} with \alpha ~ 3.3 (isolated) and ~3.5 (haloes in clusters). For M_h=10^12M_sun the rms deviations from these relations are \Delta logM_h=0.12 and 0.18, respectively. Approximately 30% of the haloes are contained within larger haloes or have massive companions (larger than ~0.3 the mass of the current halo) within 3 virial radii. The remaining 70% of the haloes are isolated objects. The distribution of \beta as well as the concentration-mass and M_h-V_m relations for the isolated haloes agree very well with the predictions of our seminumerical approach which is based on a generalization of the secondary infall model and on the extended Press-Schechter formalism.Comment: 14 pages, 11 figures included, uses mn.sty, accepted by MNRAS. Minor modifications, new and updated reference

Draft Genome Sequences of Two Gammaproteobacterial Methanotrophs Isolated from Rice Ecosystems

The genomes of the aerobic methanotrophs “Methyloterricola oryzae” strain 73aT and Methylomagnum ishizawai strain 175 were sequenced. Both strains were isolated from rice plants. Methyloterricola oryzae strain 73aT represents the first isolate of rice paddy cluster I, and strain 175 is the second representative of the recently described genus Methylomagnum

Early High-Dose Vitamin D3 for Critically Ill, Vitamin D-Deficient Patients

BACKGROUND: Vitamin D deficiency is a common, potentially reversible contributor to morbidity and mortality among critically ill patients. The potential benefits of vitamin D supplementation in acute critical illness require further study. METHODS: We conducted a randomized, double-blind, placebo-controlled, phase 3 trial of early vitamin D3 supplementation in critically ill, vitamin D-deficient patients who were at high risk for death. Randomization occurred within 12 hours after the decision to admit the patient to an intensive care unit. Eligible patients received a single enteral dose of 540,000 IU of vitamin D3 or matched placebo. The primary end point was 90-day all-cause, all-location mortality. RESULTS: A total of 1360 patients were found to be vitamin D-deficient during point-of-care screening and underwent randomization. Of these patients, 1078 had baseline vitamin D deficiency (25-hydroxyvitamin D level,[50 nmol per liter]) confirmed by subsequent testing and were included in the primary analysis population. The mean day 3 level of 25-hydroxyvitamin D was 46.9±23.2 ng per milliliter (117±58 nmol per liter) in the vitamin D group and 11.4±5.6 ng per milliliter (28±14 nmol per liter) in the placebo group (difference, 35.5 ng per milliliter; 95% confidence interval [CI], 31.5 to 39.6). The 90-day mortality was 23.5% in the vitamin D group (125 of 531 patients) and 20.6% in the placebo group (109 of 528 patients) (difference, 2.9 percentage points; 95% CI, -2.1 to 7.9; P = 0.26). There were no clinically important differences between the groups with respect to secondary clinical, physiological, or safety end points. The severity of vitamin D deficiency at baseline did not affect the association between the treatment assignment and mortality. CONCLUSIONS: Early administration of high-dose enteral vitamin D3 did not provide an advantage over placebo with respect to 90-day mortality or other, nonfatal outcomes among critically ill, vitamin D-deficient patients. METHODS: We conducted a randomized, double-blind, placebo-controlled, phase 3 trial of early vitamin D3 supplementation in critically ill, vitamin D-deficient patients who were at high risk for death. Randomization occurred within 12 hours after the decision to admit the patient to an intensive care unit. Eligible patients received a single enteral dose of 540,000 IU of vitamin D3 or matched placebo. The primary end point was 90-day all-cause, all-location mortality

P003 Randomised, placebo-controlled study of solriamfetol for excessive daytime sleepiness in narcolepsy types 1/2

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