Clinicopathological And Immunohistochemical Evaluation Of Oral And Oropharyngeal Squamous Cell Carcinoma In Chilean Population

In oral and oropharyngeal squamous cell carcinoma (OCSCC and OPSCC) exist an association between clinical and histopathological parameters with cell proliferation, basal lamina, connective tissue degradation and surrounding stroma markers. We evaluated these associations in Chilean patients. A convenience sample of 37 cases of OCSCC (n=16) and OPSCC (n=21) was analyzed clinically (TNM, clinical stage) and histologically (WHO grade of differentiation, pattern of tumor invasion). We assessed the expression of p53, Ki67, HOXA1, HOXB7, type IV collagen (ColIV) and carcinoma-associated fibroblast (a-SMA-positive cells). Additionally we conducted a univariate/bivariate analysis to assess the relationship of these variables with survival rates. Males were mostly affected (56.2% OCSCC, 76.2% OPSCC). Patients were mainly diagnosed at III/IV clinical stages (68.8% OCSCC, 90.5% OPSCC) with a predominantly infiltrative pattern invasion (62.9% OCSCC, 57.1% OPSCC). Significant association between regional lymph nodes (N) and clinical stage with OCSCC-HOXB7 expression (Chi-Square test P < 0.05) was observed. In OPSCC a statistically significant association exists between p53, Ki67 with gender (Chi-Square test P < 0.05). In OCSCC and OPSCC was statistically significant association between ki67 with HOXA1, HOXB7, and between these last two antigens (Pearson's Correlation test P < 0.05). Furthermore OPSCC-p53 showed significant correlation when it was compared with a-SMA (Kendall's Tau-c test P < 0.05). Only OCSCC-pattern invasion and OPSCC-primary tumor (T) pattern resulted associated with survival at the end of the follow up period (Chi-Square Likelihood Ratio, P < 0.05). Clinical, histological and immunohistochemical features are similar to seen in other countries. Cancer proliferation markers were associated strongly from each other. Our sample highlights prognostic value of T and pattern of invasion, but the conclusions may be limited and should be considered with caution (small sample). Many cases were diagnosed in the advanced stages of the disease, which suggests that the diagnosis of OCSCC and OPSCC is made late.7959685977Wang, Q., Gao, P., Wang, X., Duan, Y., Investigation and identification of potential biomarkers in human saliva for the early diagnosis of oral squamous cell carcinoma (2013) Clin Chim Acta, 427 C, pp. 79-85Parkin, D., Bray, F., Ferlay, J., Pisani, P., Global cancer statistics, 2002 (2005) CA Cancer J Clin, 55, p. 74Dissanayaka, W.L., Pitiyage, G., Kumarasiri, P.V., Liyanage, R.L., Dias, K.D., Tilakaratne, W.M., Clinical and histopathologic parameters in survival of oral squamous cell carcinoma (2012) Oral Surg Oral Med Oral Pathol Oral Radiol, 113, pp. 518-525Koontongkaew, S., The tumor microenvironment contribution to development, growth, invasion and metastasis of head and neck squamous cell carcinomas (2013) J Cancer, 4, p. 66Dalianis, T., Human papillomavirus and oropharyngeal cancer, the epidemics, and significance of additional clinical biomarkers for prediction of response to therapy (2014) Int J Oncol, 44, pp. 1799-1805Kostareli, E., Holzinger, D., Hess, J., New concepts for translational head and neck oncology: Lessons from HPV-related oropharyngeal squamous cell carcinomas (2012) Front Oncol, 2, p. 36Rivera, C., Venegas, B., Histological and molecular aspects of oral squamous cell carcinoma (2014) Oncol Lett, 8, pp. 7-11Bryne, M., Koppang, H.S., Lilleng, R., Kjærheim, Å., Malignancy grading of the deep invasive margins of oral squamous cell carcinomas has high prognostic value (2005) J Pathol, 166, pp. 375-381Santos-Garcia, A., Abad-Hernandez, M.M., Fonseca-Sanchez, E., Julian-Gonzalez, R., Galindo-Villardon, P., Cruz-Hernandez, J.J., Bullon-Sopelana, A., E-cadherin, laminin and collagen IV expression in the evolution from dysplasia to oral squamous cell carcinoma (2006) Med Oral Patol Oral Cir Bucal, 11, pp. E100-E105Ramqvist, T., Dalianis, T., Oropharyngeal cancer epidemic and human papillomavirus (2010) Emerg Infect Dis, 16, pp. 1671-1677Rodrigues, P.C., Miguel, M.C., Bagordakis, E., Fonseca, F.P., de Aquino, S.N., Santos-Silva, A.R., Lopes, M.A., Coletta, R.D., Clinicopathological prognostic factors of oral tongue squamous cell carcinoma: a retrospective study of 202 cases (2014) Int J Oral Maxillofac Surg, 43, pp. 795-801Agarwal, A., Sethi, A., Sareen, D., Dhingra, S., Oral and oropharyngeal squamous cell carcinoma in our population: the clinic-pathological and morphological description of 153 casescarcinoma de Células Escamosas Oral y Orofaríngeo en Nuestra Población: Descripción Clínico-Patológica y Morfológica de 153 Casos (2011) Int J Morphol, 29, pp. 686-693Roosli, C., Tschudi, D.C., Studer, G., Braun, J., Stoeckli, S.J., Outcome of patients after treatment for a squamous cell carcinoma of the oropharynx (2009) Laryngoscope, 119, pp. 534-540Riera, P., Martinez, B., [Morbidity and mortality for oral and pharyngeal cancer in Chile] (2005) Rev Med Chil, 133, pp. 555-563Marsh, D., Suchak, K., Moutasim, K.A., Vallath, S., Hopper, C., Jerjes, W., Upile, T., Thomas, G.J., Stromal features are predictive of disease mortality in oral cancer patients (2011) J Pathol, 223, pp. 470-481Woolgar, J., Rogers, S., West, C., Errington, R., Brown, J., Vaughan, E., Survival and patterns of recurrence in 200 oral cancer patients treated by radical surgery and neck dissection (1999) Oral Oncol, 35, pp. 257-265Bórquez, P., Capdeville, F., Madrid, A., Veloso, M., Cárcamo, M., Sobrevida global y por estadios de 137 pacientes con cáncer intraoral: experiencia del Instituto Nacional del CáncerAnalysis of survival of 137 patients with oral cancer (2011) Rev Chil Cir, 63, pp. 351-355Woolgar, J.A., Triantafyllou, A., Pitfalls and procedures in the histopathological diagnosis of oral and oropharyngeal squamous cell carcinoma and a review of the role of pathology in prognosis (2009) Oral Oncol, 45, pp. 361-385Li, Y., Bai, S., Carroll, W., Dayan, D., Dort, J.C., Heller, K., Jour, G., Brandwein-Gensler, M., Validation of the risk model: high-risk classification and tumor pattern of invasion predict outcome for patients with low-stage oral cavity squamous cell carcinoma (2013) Head Neck Pathol, 7, pp. 211-223Chang, Y.C., Nieh, S., Chen, S.F., Jao, S.W., Lin, Y.L., Fu, E., Invasive pattern grading score designed as an independent prognostic indicator in oral squamous cell carcinoma (2010) Histopathology, 57, pp. 295-303Adeyemi, B.F., Olusanya, A.A., Lawoyin, J.O., Oral squamous cell carcinoma, socioeconomic status and history of exposure to alcohol and tobacco (2011) J Natl Med Assoc, 103, pp. 498-502De Souza Setubal Destro, M.F., Bitu, C.C., Zecchin, K.G., Graner, E., Lopes, M.A., Kowalski, L.P., Coletta, R.D., Overexpression of HOXB7 homeobox gene in oral cancer induces cellular proliferation and is associated with poor prognosis (2010) Int J Oncol, 36, pp. 141-149Bitu, C.C., Destro, M.F., Carrera, M., Da Silva, S.D., Graner, E., Kowalski, L.P., Soares, F.A., Coletta, R.D., HOXA1 is overexpressed in oral squamous cell carcinomas and its expression is correlated with poor prognosis (2012) BMC Cancer, 12, p. 146Liao, W.T., Jiang, D., Yuan, J., Cui, Y.M., Shi, X.W., Chen, C.M., Bian, X.W., Ding, Y.Q., HOXB7 as a prognostic factor and mediator of colorectal cancer progression (2011) Clin Cancer Res, 17, pp. 3569-357

Mapping cell-in-cell structures in oral squamous cell carcinoma

Cell-in-cell (CIC) structures contribute to tumor aggressiveness and poor prognosis in oral squamous cell carcinoma (OSCC). In vitro 3D models may contribute to the understanding of the underlying molecular mechanisms of these events. We employed a spheroid model to study the CIC structures in OSCC. Spheroids were obtained from OSCC (HSC3) and cancer-associated fibroblast (CAF) lines using the Nanoshuttle-PLTM bioprinting system (Greiner Bio-One). Spheroid form, size, and reproducibility were evaluated over time (EvosTM XL; ImageJ version 1.8). Slides were assembled, stained (hematoxylin and eosin), and scanned (Axio Imager Z2/VSLIDE) using the OlyVIA System (Olympus Life Science) and ImageJ software (NIH) for cellular morphology and tumor zone formation (hypoxia and/or proliferative zones) analysis. CIC occurrence, complexity, and morphology were assessed considering the spheroid regions. Well-formed spheroids were observed within 6 h of incubation, showing the morphological aspects of the tumor microenvironment, such as hypoxic (core) and proliferative zone (periphery) formation. CIC structures were found in both homotypic and heterotypic groups, predominantly in the proliferative zone of the mixed HSC3/CAF spheroids. “Complex cannibalism” events were also noted. These results showcase the potential of this model in further studies on CIC morphology, formation, and relationship with tumor prognosis

Insulin-like growth factor 1 gene (CA)n repeats and a variable number of tandem repeats of the insulin gene in Brazilian children born small for gestational age

OBJECTIVE: To investigate the influence of (CA)n repeats in the insulin-like growth factor 1 gene and a variable number of tandem repeats of the insulin gene on birth size in children who are small or adequate-sized for gestational age and to correlate these polymorphisms with serum insulin-like growth factor 1 levels and insulin sensitivity in children who are small for gestational age, with and without catch-up growth. PATIENTS AND METHODS: We evaluated 439 infants: 297 that were adequate-sized for gestational age and 142 that were small for gestational age (66 with and 76 without catch-up). The number of (CA)n repeat in the insulin-like growth factor 1 gene and a variable number of tandem repeats in the insulin gene were analyzed using GENESCAN software and polymerase chain reaction followed by enzymatic digestion, respectively. Clinical and laboratory data were obtained from all patients. RESULTS: The height, body mass index, paternal height, target height and insulin-like growth factor 1 serum levels were higher in children who were small for gestational age with catch-up. There was no difference in the allelic and genotypic distributions of both polymorphisms between the adequate-sized and small infants or among small infants with and without catch-up. Similarly, the polymorphisms were not associated with clinical or laboratory variables. CONCLUSION: Polymorphisms of the (CA)n repeats of the insulin-like growth factor 1 gene and a variable number of tandem repeats of the insulin gene, separately or in combination, did not influence pre- or postnatal growth, insulin-like growth factor 1 serum levels or insulin resistance

Machine learning application for prediction of locoregional recurrences in early oral tongue cancer: a Web-based prognostic tool

Estimation of risk of recurrence in early-stage oral tongue squamous cell carcinoma (OTSCC) remains a challenge in the field of head and neck oncology. We examined the use of artificial neural networks (ANNs) to predict recurrences in early-stage OTSCC. A Web-based tool available for public use was also developed. A feedforward neural network was trained for prediction of locoregional recurrences in early OTSCC. The trained network was used to evaluate several prognostic parameters (age, gender, T stage, WHO histologic grade, depth of invasion, tumor budding, worst pattern of invasion, perineural invasion, and lymphocytic host response). Our neural network model identified tumor budding and depth of invasion as the most important prognosticators to predict locoregional recurrence. The accuracy of the neural network was 92.7%, which was higher than that of the logistic regression model (86.5%). Our online tool provided 88.2% accuracy, 71.2% sensitivity, and 98.9% specificity. In conclusion, ANN seems to offer a unique decision-making support predicting recurrences and thus adding value for the management of early OTSCC. To the best of our knowledge, this is the first study that applied ANN for prediction of recurrence in early OTSCC and provided a Web-based tool.</p

Peutz-jeghers Syndrome In A 14-year-old Boy: Case Report And Review Of The Literature

Peutz-Jeghers syndrome (PSJ) is a relatively rare but well-recognized condition, with a prevalence of approximately one in 120 000 births in the USA. It is generally inherited as an autosomal dominant trait, although 35% of cases are new mutations. This disorder is characterized by melanocytic macules on the hands, feet, peri-oral skin and oral mucosa, and multiple gastrointestinal hamartomatous polyps. People with PSJ have an increased risk for developing a variety of malignant tumours. The aim of the present study was to report one case of PSJ in a 14-year-old boy with mucocutaneous pigmentation associated with duodenal hamartomatous polyps. © 2005 BSPD and IAPD.153224228Lindor, N.M., Greene, M.H., The concise handbook of family cancer syndromes. Mayo Familial Cancer Program (1998) Journal of the National Cancer Institute, 90, pp. 1039-1071McGarrity, T.J., Kulin, H.E., Zaino, R.J., Peutz-Jeghers syndrome. Clinical reviews (2000) The American Journal of Gastroenterology, 95, pp. 596-604Peutz, J.L.A., A very remarkable case of familial polyposis of mucous membranes of the intestinal tract and naso-pharynx accompanied by peculiar pigmentation of skin and mucous membrane (1921) Nederlands Tijdschrift Voor Geneeskunde, 10, pp. 134-146Jeghers, H., McKusick, V.A., Katz, K.H., Generalized intestinal polyposis and melanin spots of the oral mucosa, lips and digits: A syndrome of diagnostic significance (1949) The New England Journal of Medicine, 241, pp. 993-1031Bruwer, A., Bargen, J.A., Kierland, R.R., Surface pigmentation and generalized intestinal polyposis (Peutz-Jeghers syndrome) (1954) Mayo Clinic Proceedings, 29, p. 168Tovar, J.A., Eizaguirre, I., Albert, A., Jimenez, J., Peutz-Jeghers syndrome in children: Report of two cases and review of the literature (1983) Journal of Pediatric Surgery, 18, pp. 1-6Tomlinson, I.P.M., Houlston, R.S., Peutz-Jeghers syndrome (1997) Journal of Medical Genetics, 34, pp. 1007-1011Andersen, M.D., The Network for Peutz-Jeghers and Juvenile Polyposis Syndrome, , http://www.epigenetic.org/pjs/, WWW documentNeville, B.W., Damm, D.D., Allen, C.M., Bouquot, J.E., (2002) Oral and Maxillofacial Pathology, 2nd Edn., pp. 653-654. , Philadelphia, PA: W. B. SaundersGiardiello, F.M., Welsh, S.B., Hamilton, S.R., Increased risk of cancer in the Peutz-Jeghers syndrome (1987) The New England Journal of Medicine, 316, pp. 1511-1514Sasaki, T., Fukumori, D., Sato, M., Sakai, K., Ohmori, H., Yamamoto, F., Peutz-Jeghers syndrome associated with intestinal intussusception: A case report (2002) International Surgery, 87, pp. 256-259Dippolito, A.D., Aburano, A., Bezouska, C.A., Happ, R.A., Enteritis cystica profunda in Peutz-Jeghers syndrome. Report of a case and review of the literature (1987) Diseases of the Colon and Rectum, 30, pp. 192-198Perzin, K.H., Bridge, M.F., Adenomatous and carcinomatous changes in hamartomatous polyps of the small intestine (Peutz-Jeghers syndrome): Report of a case and review of the literature (1982) Cancer, 49, pp. 971-983Patterson, M., Kernen, J.A., Epithelioid leiomyosarcoma originating in a hamartomatous polyp from a patient with Peutz-Jeghers syndrome (1985) Gastroenterology, 88, pp. 1060-1064Linos, D.A., Dozois, R.R., Dahlin, D.C., Bartholomew, L.G., Does Peutz-Jeghers syndrome predispose to gastrointestinal malignancy? (1981) Archives of Surgery, 116, pp. 1182-1184Spigelman, A.D., Murday, V., Phillips, R.K.S., Cancer and the Peutz-Jeghers syndrome (1989) Gut, 30, pp. 1588-1590Rodu, B., Martinez Jr., M.G., Peutz-Jeghers syndrome and cancer (1984) Oral Surgery, Oral Medicine and Oral Pathology, 58, pp. 584-588Hemminki, A., Tomlinson, I., Markie, D., Localization of a susceptibility locus for Peutz-Jeghers syndrome to 19p using comparative genomic hybridization and targeted linkage analysis (1997) Nature Genetics, 15, pp. 87-90Hemminki, A., Markie, D., Tomlinson, I., A serine/threonine kinase gene defective in Peutz-Jeghers syndrome (1998) Nature, 391, pp. 184-187Jenne, D.E., Reimann, H., Nezu, J., Peutz-Jeghers syndrome is caused by mutations in a novel serine threonine kinase (1998) Nature Genetics, 18, pp. 38-4

Sclerotherapy Followed By Surgery For The Treatment Of Oral Hemangioma: A Report Of Two Cases

Hemangiomas, vascular malformations, and varices are common benign vascular lesions in the head and neck region. They can occur in the mouth and primarily affect the lips, tongue, buccal mucosa, and palate. The main types of treatments are surgery and intralesional injection of sclerosant agents. However, other therapies have been considered, such as systemic corticosteroids, laser therapy, interferon a, and cryotherapy. Currently, sclerotherapy is employed largely because of its efficiency and ability to conserve the surrounded tissues. Surgery can be used exclusively or associated with sclerotherapy in lesions that do not show complete resolution. This article describes the cases of two patients with oral hemangiomas that were submitted to sclerotherapy with ethanol-amine oleate. Although an important decrease was detected after seven applications in both cases, surgical resection of the residual lesion was performed to achieve optimal results.593e121e125Moukaddam, H., Pollak, J., Haims, A.H., MRI characteristics and classification of peripheral vascular malformations and tumors (2009) Skeletal Radiol, 38 (6), pp. 535-547Finn, M.C., Glowacki, J., Mulliken, J.B., Congenital vascular lesions: Clinical application of a new classification (1983) Journal of Pediatric Surgery, 18 (6), pp. 894-900Takahashi, K., Mulliken, J.B., Kozakewich, H.P.W., Rogers, R.A., Folkman, J., Ezekowitz, R.A.B., Cellular markers that distinguish the phases of hemangioma during infancy and childhood (1994) Journal of Clinical Investigation, 93 (6), pp. 2357-2364Al Buainian, H., Verhaeghe, E., Dierckxsens, L., Naeyaert, J.M., Early treatment of hemangiomas with lasers (2003) A review, Dermatology, 206 (4), pp. 370-373Liu, Y., Liu, D., Wang, Y., Zhang, W., Zhao, F., Clinical study of sclerotherapy of maxillofacial venous malformations using absolute ethanol and pingyangmycin (2009) J Oral Maxillofac Surg, 67 (1), pp. 98-104Mulliken, J.B., Glowacki, J., Thomson, H.G., Hemangiomas and vascular malformations in infants and children: A classification based on endothelial characteristics (1982) Plastic and Reconstructive Surgery, 69 (3), pp. 412-422Brunelle, F.O., Chaumont, P., Jeillac, D., Manach, Y., Lallemand, D., Facial vascular malformations in children. Conventional and digital, diagnostic and therapeutic angiography (1988) Pediatr Radiol, 18 (5), pp. 377-382Selim, H., Selim, A., Khachemoune, A., Metwally, S.A.F.A., Use of sclerosing agent in the management of oral and perioral hemangiomas: Review and case reports (2007) Medical Science Monitor, 13 (9), pp. CS114-CS119. , http://www.medscimonit.com/pub/vol_13/no_9/10040.pdfCorrea, P.H., Nunes, L.C., Johann, A.C., Aguiar, M.C., Gomez, R.S., Mesquita, R.A., Prevalence of oral hemangioma, vascular malformation and varix in a Brazilian population (2007) Braz Oral Res, 21 (1), pp. 40-45Corbet, E.F., Holmgren, C.J., Phillipsen, H.P., Oral mucosal lesions in 65-74-year-old Hong Kong Chinese (1994) Community Dent Oral Epidemiol, 22 (5 PART 2), pp. 392-395Espinoza, I., Rojas, R., Aranda, W., Gamonal, J., Prevalence of oral mucosal lesions in elderly people in Santiago, Chile (2003) Journal of Oral Pathology and Medicine, 32 (10), pp. 571-575. , DOI 10.1034/j.1600-0714.2003.00031.xAl-Khateeb, T., Hamasha, A.A.-H., Almasri, N.M., Oral and maxillofacial tumours in North Jordanian children and adolescents: A retrospective analysis over 10 years (2003) International Journal of Oral and Maxillofacial Surgery, 32 (1), pp. 78-83. , DOI 10.1054/ijom.2002.0309Barrett, A.W., Speight, P.M., Superficial arteriovenous hemangioma of the oral cavity (2000) Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 90 (6), pp. 731-738Legiehn, G.M., Heran, M.K., Venous malformations: Classification, development, diagnosis, and interventional radiologic management (2008) Radiol Clin North Am, 46 (3), pp. 545-597Van Doorne, L., De Maeseneer, M., Stricker, C., Vanrensbergen, R., Stricker, M., Diagnosis and treatment of vascular lesion of the lip (2002) British Journal of Oral and Maxillofacial Surgery, 40 (6), pp. 497-503. , DOI 10.1016/S0266-4356(02)00153-5Rodrigues Johann, A.C.B., Ferreira Aguiar, M.C., Vieira Do Carmo, M.A., Gomez, R.S., Castro, W.H., Mesquita, R.A., Sclerotherapy of benign oral vascular lesion with ethanolamine oleate: An open clinical trial with 30 lesions (2005) Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontology, 100 (5), pp. 579-584. , DOI 10.1016/j.tripleo.2004.12.021, PII S1079210405000338Deveikis, J.R., Percutaneous ethanol sclerotherapy for vascular malformations in the head and neck (2005) Arch Facial Plast Surg, 7 (5), pp. 322-325Berenguer, B., Burrows, P.E., Zurakowski, D., Mulliken, J.B., Sclerotherapy of craniofacial venous malformations: Complications and results (1999) Plast Reconstr Surg, 104 (1), pp. 1-11Jin, Y., Lin, X., Li, W., Hu, X., Ma, G., Wang, W., Sclerotherapy after embolization of draining vein: A safe treatment method for venous malformations (2008) J Vase Surg, 47 (6), pp. 1292-1299Boye, E., Jinnin, M., Olsen, B.R., Infantile hemangioma: Challenges, new insights, and therapeutic promise (2009) J Craniofac Surg, 20 (SUPPL. 1), pp. 678-684Zhao, J.H., Zhang, W.F., Zhao, Y.F., Sclerotherapy of oral and facial venous malformations with use of pingyangmycin and/or sodium morrhate (2004) Int J Oral Maxillofac Surg, 33 (5), pp. 463-466Kaplan, I., Gassner, S., Shindel, Y., Carbon dioxide laser in head and neck surgery (1974) Am J Surg, 128 (4), pp. 543-544Andrews, G.C., Kelly, R.J., Treatment of vascular nevi by injection of sclerosing solutions (1932) Arch Dermatol, 26, pp. 92-94O'Donovan, J.C., Donaldson, J.S., Morello, F.P., Pensler, J.M., Vogelzang, R.L., Bauer, B., Symptomatic hemangiomas and venous malformations in infants, children, and young adults: Treatment with percutaneous injection of sodium tetradecil sulfate (1997) AJR Am J Roentgenol, 169 (3), pp. 723-729Winter, H., Drager, E., Sterry, W., Sclerotherapy for treatment of hemangiomas (2000) Dermatologic Surgery, 26 (2), pp. 105-108. , DOI 10.1046/j.1524-4725.2000.98012.xMatsumoto, K., Nakanishi, H., Koizumi, Y., Seike, T., Kanda, I., Kubo, Y., Sclerotherapy of hemangiomas with late involution (2003) Dermatol Surg, 29 (6), pp. 668-671Kaessler, H.W., Vascular birth marks (1934) J Am Med Assoc, 110, pp. 1644-1647Choi, Y.H., Han, M.H., O-Ki, K., Cha, S.H., Chang, K.-H., Craniofacial cavernous venous malformations: Percutaneous sclerotherapy with use of ethanolamine oleate (2002) Journal of Vascular and Interventional Radiology, 13 (5), pp. 475-482Bordas, J.M., Feu, F., Vilella, A., Rodes, J., Anaphylactic reaction to ethanolamine oleate injection in sclerotherapy of esophageal varices (1989) Endoscopy, 21 (1), p. 5

Matrix Metalloproteinase-2 And -9 Activities Correlate With The Disease-free Survival Of Oral Squamous Cell Carcinoma Patients.

The aim of the present study was to determine whether matrix metalloproteinase (MMP) activities are associated with the clinicopathological features of oral squamous cell carcinomas (OSCCs). Forty-four samples of primary OSCCs were microdissected, incubated in serum-free cell culture medium for 16 h, and the activity of secreted MMPs analyzed by gelatin zymography and densitometry. Two major enzymes with gelatinolytic activity were produced by all tumor samples and characterized as MMP-2 and -9 by their molecular weight, immunoprecipitation using monoclonal antibodies, and specific inhibition by 1,10-phenanthroline or EDTA. Patients with tumors showing elevated activity of MMP-2 and -9 had shorter disease-free survival after treatment than patients with tumors exhibiting low MMP activities. These results suggest that the zymographic measurement of the MMP-2 and -9 activities in OSCC specimens may be useful to predict the disease-free survival period of patients affected by these tumors.20118919

Expression And Activity Of Matrix Metalloproteinase-2 (mmp-2) In The Development Of Rat First Molar Tooth Germ.

Tooth germ development is associated with morphological and biochemical changes of the dental papilla and enamel organ. Enzymes with gelatinolytic activities were studied by semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR) and enzymography in tooth germ of newborn to 15-day-old rats. Three major bands with gelatinolytic activity were detected at all periods and characterized as the latent and active forms of MMP-2 using their molecular weight and activity dependent on Zn++ and Ca++ ions as criteria. Expression and activity of MMP-2 increased progressively from 0 to 15 days after birth. Mechanical separation of the tooth germ from 10-day-old rats showed that the gelatinolytic activity was localized mainly in the dental papilla and not the dental organ. These data indicate that the expression and activity of MMP-2 varies during the development and maturation of rat first molar tooth germ.1329710