Multiple mapping conditioning in homogeneous reacting flows

Multiple mapping conditioning (MMC) is used to model local extinction and reignition phenomena in homogeneous, isotropic decaying turbulence. It is recognized that mixture fraction alone is not sufficient to account for turbulent scalar fluctuations and that more than one reference variable needs to be introduced. We introduce a second reference variable with a dual character: the second variable is a dissipation-like variable that emulates the intermittent behaviour of scalar dissipation and it is therefore the cause for local extinction in our modelling. However, the second variable is also used to match the scalar variance of a reaction progress variable to ensure consistency in temperature flucutations of the MMC model and Direct Numerical Simulations. The resulting model provides a (fully) closed formulation for the modelling of local extinction and re-ignition events and predictions of the joint probability distribution of mixture fraction and sensible enthalpy, of reactive species and of the global conversion rates are good and clear improvments over conventional mixture fraction based methods that use mixture fraction as the only conditioning paramenter

Hyponatremia in Endurance Athletes

The purpose of this paper was to identify the relationship between hyponatremia, or water intoxication, and endurance athletes. Athletic trainers and paramedics must be educated about this potentially fatal problem and be able to determine the appropriate treatment for a hyponatremic athlete

Results of tests of a 0.010- and 0.015-scale models of space shuttle orbiter configurations 3 and 3A in the Ames Research Center 3.5 foot hypersonic wind tunnel (OA23)

Longitudinal and lateral-directional stability and control characteristics were evaluated at Mach numbers of 5.3, 7.3 and 10.3 at angles of attack up to 50 degrees with Beta = 0 degrees and, for a few cases, Beta = 5 degrees. Component force data, fuselage base pressures and shadowgraph patterns were recorded

Cryogenic microstripline-on-Kapton microwave interconnects

Simple broadband microwave interconnects are needed for increasing the size of focal plane heterodyne radiometer arrays. We have measured loss and cross-talk for arrays of microstrip transmission lines in flex circuit technology at 297 and 77 K, finding good performance to at least 20 GHz. The dielectric constant of Kapton substrates changes very little from 297 to 77 K, and the electrical loss drops. The small cross-sectional area of metal in a printed circuit structure yields overall thermal conductivities similar to stainless steel coaxial cable. Operationally, the main performance tradeoffs are between crosstalk and thermal conductivity. We tested a patterned ground plane to reduce heat flux.Comment: 3 pages, 3 figures, submitted to The Review of Scientific Instrument

A multiple mapping conditioning model for differential diffusion

This work introduces modeling of differential diffusion within the multiple mapping conditioning (MMC) turbulent mixing and combustion framework. The effect of differential diffusion on scalar variance decay is analyzed and, following a number of publications, is found to scale as Re. The ability to model the differential decay rates is the most important aim of practical differential diffusion models, and here this is achieved in MMC by introducing what is called the side-stepping method. The approach is practical and, as it does not involve an increase in the number of MMC reference variables, economical. In addition we also investigate the modeling of a more refined and difficult to reproduce differential diffusion effect - the loss of correlation between the different scalars. For this we develop an alternative MMC model with two reference variables but which also makes use of the side-stepping method. The new models are successfully validated against DNS results available in literature for homogenous, isotropic two scalar mixing

Differential positive control by Oct-1 and Oct-2: activation of a transcriptionally silent motif through Oct-1 and VP16 corecruitment

Transcriptional regulation by the ubiquitous human POU homeo domain protein Oct-1 and the related B-cell protein Oct-2 is a model for understanding how proteins that recognize the same regulatory site elicit different programs of gene transcription. Here, we describe a mechanism for differential promoter activation whereby only Oct-1, through selective corecruitment with the herpesvirus trans-activator VP16, acquires the ability to stimulate transcription from a TAATGARAT-containing site that responds to neither Oct-1 nor Oct-2 alone. To measure differential in vivo activation by human Oct-1 and Oct-2 in response to VP16, we have developed a transient assay in murine NIH-3T3 cells. Surprisingly, murine Oct-1 associates with VP16 much less effectively than its human counterpart, most likely because the murine Oct-1 homeo domain differs at four positions from the human Oct-1 homeo domain. The murine cell transient assay shows directly that human Oct-1, but not human Oct-2, can respond to VP16 in vivo. The Oct-1 DNA-binding POU domain is sufficient and the Oct-1 homeo domain is critical for this response, because an Oct-1 POU domain containing the Oct-2 homeo domain fails to respond to the VP16-induced positive control of transcription. Thus, by selective homeo domain interaction and corecruitment to an otherwise silent regulatory element, VP16 expands the repertoire of sites responsive to Oct-1 without affecting the activity of its close relative Oct-2

A single amino acid exchange transfers VP16-induced positive control from the Oct-1 to the Oct-2 homeo domain

The selective association of the herpesvirus trans-activator VP16 with the human Oct-1 homeo domain is a model for differential positive transcriptional control by homeo domains. VP16 discriminates between the closely related homeo domains of Oct-1 and Oct-2 by distinguishing among their seven amino-acid differences; these differences lie on the surface that is thought to be accessible when the homeo domain is bound to DNA. Only two of these seven differences are recognized by VP16, one in each of the first two alpha-helices of the tri-alpha-helical homeo domain. The major determinant for selective association with VP16 in vitro and VP16-induced positive control in vivo is a single glutamic acid residue at position 22 in the first alpha-helix of the Oct-1 homeo domain, but the acidic properties of this residue are not critical for association with VP16 in vitro or in vivo, because it can be replaced by glutamine with little or no deleterious effect. Mere replacement of the single corresponding alanine residue in the Oct-2 homeo domain with the key glutamic acid residue is sufficient to confer on the Oct-2 homeo domain the ability to associate with VP16 in vitro and respond to VP16-induced positive control in vivo. Thus, the specificity of homeo domain positive control can be conferred by a single amino acid difference

A review of malaria epidemiology and control in Papua New Guinea 1900 to 2021: progress made and future directions

The research and control of malaria has a long history in Papua New Guinea, sometimes resulting in substantial changes to the distribution of infection and transmission dynamics in the country. There have been four major periods of malaria control in PNG, with the current control programme having commenced in 2004. Each previous control programme was successful in reducing malaria burden in the country, but multiple factors led to programme failures and eventual breakdown. A comprehensive review of the literature dating from 1900 to 2021 was undertaken to summarize control strategies, epidemiology, vector ecology and environmental drivers of malaria transmission in PNG. Evaluations of historical control programs reveal poor planning and communication, and di culty in sustaining financial investment once malaria burden had decreased as common themes in the breakdown of previous programs. Success of current and future malaria control programs in PNG is contingent on adequate planning and management of control programs, effective communication and engagement with at-risk populations, and cohesive targeted approaches to sub-national and national control and elimination

Severe Heat Cramps in a High School Football Player: A Case Report

We present a case study of an adolescent football player who suffered from severe full body muscle cramping after supplementing with creatine for two months. A paucity of data exists regarding the safety of creatine supplementation and its side effects on dehydration, body fluid/electrolyte balance, and other heat illnesses