Diagnostic value of anti-human citrullinated fibrinogen ELISA and comparison with four other anti-citrullinated protein assays

We studied the diagnostic performance of the anti-human citrullinated fibrinogen antibody (AhFibA) ELISA for rheumatoid arthritis (RA) in a consecutive cohort (population 1) and evaluated the agreement between the AhFibA ELISA and four other assays for anti-citrullinated protein/peptide antibodies (ACPAs) as well as rheumatoid factor in patients with longstanding RA (population 2). Population 1 consisted of 1024 patients with rheumatic symptoms; serum samples from these patients were sent to our laboratory for ACPA testing within the context of a diagnostic investigation for RA. Ninety-two of these patients were classified as having RA according to the American College of Rheumatology criteria and 463 were classified as non-RA patients. Population 2 consisted of 180 patients with longstanding RA and was used to assess agreement and correlations between five ACPA assays: anti-cyclic citrullinated peptide (CCP)1 and anti-CCP2 antibodies were detected using a commercially available ELISA, AhFibA using ELISA, and anti-PepA and anti-PepB antibodies using line immunoassay. Applying previously proposed cut-offs for AhFibA, we obtained a sensitivity of 60.9% and a specificity of 98.7% in population 1. Receiver operating characteristic curve analysis could not detect a significant difference in diagnostic performance between the AhFibA ELISA and anti-CCP2 assay. Performing a hierarchical nearest neighborhood cluster analysis of the five different ACPA assays in population 2, we identified two clusters: a cluster of anti-pepA, anti-pepB and anti-CCP1, and a cluster of AhFibA and anti-CCP2. In conclusion, we found that AhFibA and anti-CCP2 antibodies had similar diagnostic performance. However, disagreement between ACPA tests may occur

Controverse : Professionnaliser la formation initiale des avocats ?

International audienc

Auto-immunité anti-protéines citrullinées et physiopathologie de la polyarthrite rhumatoïde

TOULOUSE3-BU Sciences (315552104) / SudocSudocFranceF

Auto- immunité anti-protéines désiminées (la présence d'antigènes-cibles dans le tissu synovial est-elle spécifique de la polyarthrite rhumatoïde?)

Les auto-anticorps anti-protéines citrullinées (ou désiminées), spécifiques de la polyarthrite rhumatoïde (PR), et leurs antigènes-cibles articulaires, les chaînes a et b désiminées de la fibrine, tous deux présents dans le tissu synovial des patients atteints de PR, sont probablement à l'origine d'un conflit immunologique impliqué dans l'entretien de l'inflammation. Afin de déterminer si la présence de protéines désiminées et plus particulièrement de fibrine désiminée, dans le tissu synovial est ou non spécifique de la PR, nous les avons recherchées, par immunotransfert, dans des extraits de biopsies synoviales de patients atteints de PR ou atteints d'autres maladies rhumatologiques. La fibrine désiminée a été retrouvée dans 4 PR sur 5 et dans 3 contrôles sur 9. Sa présence n'est donc pas spécifique de la maladie rhumatoïde. Ces résultats conduisent à s'interroger sur les mécanismes d'induction de cette réponse humorale très spécifique de la PR.TOULOUSE3-BU Santé-Centrale (315552105) / SudocTOULOUSE3-BU Santé-Allées (315552109) / SudocSudocFranceF

Déterminants et outils de prévention de la contamination par le VIH sur le sol français des femmes migrantes originaires d'Afrique Subsaharienne et d'Haïti

PARIS7-Xavier Bichat (751182101) / SudocPARIS-BIUM (751062103) / SudocSudocFranceF

Of Human Criteria and Automatic Metrics: A Benchmark of the Evaluation of Story Generation

International audienceResearch on Automatic Story Generation (ASG) relies heavily on human and automatic evaluation. However, there is no consensus on which human evaluation criteria to use, and no analysis of how well automatic criteria correlate with them. In this paper, we propose to re-evaluate ASG evaluation. We introduce a set of 6 orthogonal and comprehensive human criteria, carefully motivated by the social sciences literature. We also present HANNA, an annotated dataset of 1,056 stories produced by 10 different ASG systems. HANNA allows us to quantitatively evaluate the correlations of 72 automatic metrics with human criteria. Our analysis highlights the weaknesses of current metrics for ASG and allows us to formulate practical recommendations for ASG evaluation

IgM and IgA Rheumatoid Factors Purified from Rheumatoid Arthritis Sera Boost the Fc Receptor– and Complement-Dependent Effector Functions of the Disease-Specific Anti–Citrullinated Protein Autoantibodies

International audienceRheumatoid factors (RF) and the disease-specific anti-citrullinated protein autoantibodies (ACPA) coexist in the joints of rheumatoid arthritis (RA) patients where they probably contribute to synovitis. We investigated the influence of IgM and IgA RF on the FcR- and complement-dependent effects of ACPA immune complexes (ACPA-IC). When stimulated by ACPA-IC formed in the presence of IgM RF or IgA RF fractions purified from RA serum pools, M-CSF-generated macrophages skewed their cytokine response toward inflammation, with increases in the TNF-α/IL-10 ratio and in IL-6 and IL-8 secretion, and decreases in the IL-1Ra/IL-1β ratio. In the IgM RF-mediated amplification of the inflammatory response of macrophages, the participation of an IgM receptor was excluded, notably by showing that they did not express any established receptor for IgM. Rather, this amplification depended on the IgM RF-mediated recruitment of more IgG into the ACPA-IC. However, the macrophages expressed FcαRI and blocking its interaction with IgA inhibited the IgA RF-mediated amplification of TNF-α secretion induced by ACPA-IC, showing its major implication in the effects of RF of the IgA class. LPS further amplified the TNF-α response of macrophages to RF-containing ACPA-IC. Lastly, the presence of IgM or IgA RF increased the capacity of ACPA-IC to activate the complement cascade. Therefore, specifically using autoantibodies from RA patients, the strong FcR-mediated or complement-dependent pathogenic potential of IC including both ACPA and IgM or IgA RF was established. Simultaneous FcR triggering by these RF-containing ACPA-IC and TLR4 ligation possibly makes a major contribution to RA synovitis

Individual, Sociodemographic, and Environmental Factors Related to Physical Activity During the Spring 2020 COVID-19 Lockdown

International audienceBackground: Research has shown important between-individual variations in physical activity (PA) during the COVID-19 lockdown.Objectives: The objectives of this is study are to examine the individual, sociodemographic, and environmental factors related to PA during the spring 2020 COVID-19 lockdown in France and to explore the mediating and moderating role of intention and self-efficacy toward PA in the relationships between sociodemographic/environmental variables and PA. Design: In this cross-sectional study, participants living in France (N = 386) completed an online survey between March 30 and April 10, 2020.Method: Minutes per week of moderate-to-vigorous PA during the lockdown; usual physical activity before the lockdown; and psychological (e.g., intention, self-efficacy, and autonomous and controlled motivation), sociodemographic (gender, age, and number of children), and environmental (habitat surface area and type of housing) factors were measured in the survey. Multiple linear regressions were used to investigate the role of these predictors on PA. Intention and self-efficacy were also examined as moderators and mediators of the association between sociodemographic/environmental factors and PA.Results: Usual physical activity before the COVID-19 lockdown, intention toward PA, habitat surface area, and controlled motivation significantly predicted PA during the lockdown. No mediating effects of intention or self-efficacy were found. Intention significantly moderated the association between gender and PA and the association between part-time work and PA.Conclusions: PA during the COVID-19 lockdown was mainly predicted by individual factors and notably usual PA. These results highlight the important role of habits in a highly changing context