Quantifizierung von Sperrluft-Hauptstrom-Interaktionen in einer Turbine

Die vorliegende Dissertationsschrift ist das Resultat der Tätigkeit als wissenschaftlicher Mitarbeiter am Fachgebiet GLR der TU Darmstadt. Sie befasst sich mit der Interaktion von Sperrluftströmen mit der Hauptströmung in Gasturbinen und Flugtriebwerken. Dabei zielt der Inhalt nicht nur auf die reine Beschreibung der Interaktionen, sondern auch auf die notwendigen Voraussetzungen, die ein Turbinenprüfstand zur wissenschaftlichen Beschreibung der Phänomene besitzen muss. In Gasturbinen und Flugtriebwerken herrschen im Hauptströmungskanal Heißgastemperaturen, die mehrere hundert Grad Celsius oberhalb der zulässigen Materialtemperaturen liegen. Der Hauptströmungskanal ist nicht geschlossen. Die abwechselnd stehend und rotierend angeordneten Turbinenschaufeln erfordern Spalte, um z.B. Wärmedehnungen zu ermöglichen. Durch Sperrluft wird verhindert, dass die heißen Gase durch Spalte in Bereiche außerhalb des Hauptströmungskanals eindringen und die dortigen Komponenten thermisch überlasten. Eine thermische Überlastung führt zur Minderung der Lebensdauer und kann im Extremfall zum Totalverlust von Komponenten, dem Triebwerk und ggf. auch dem Flugzeug führen. Die konservative Auslegung der Sperr- und Kühlluftsysteme hat eine höhere Ausblasung von Sperrluft in den Hauptströmungskanal als notwendig zur Folge. Dadurch entstehen zusätzliche Verluste, die im Rahmen dieser Arbeit quantifiziert werden. In der vorliegenden Schrift wird erläutert, welche Eigenschaften ein Turbinenprüfstand erfüllen muss, um mit realen Turbinen vergleichbare Ergebnisse zu erhalten. Dabei werden dimensionslose Kennzahlen realer Gasturbinen betrachtet und auf einen Prüfstand projiziert. Der daraus hervorgegangene Prüfstand wird beschrieben und durch seine wesentlichen Kenngrößen charakterisiert. Die Ergebnisse dieser Arbeit quantifizieren die Wirkungsgradänderungen, die in Gasturbinen durch veränderte Sperrluftausblasemengen resultieren. Detaillierte Strömungsfeldvermessungen liefern Informationen über die Ursachen der Wirkungsgradänderungen. Mit den Erkenntnissen kann bei zukünftigen Turbinenentwicklungen der Sperrluftbedarf reduziert werden, was einen direkten positiven Einfluss auf den Gesamtwirkungsgrad eines Triebwerkes hat. Ebenso können Strömungsverluste in einer Turbine mit Hilfe der erhaltenen Ergebnisse reduziert werden. Eine genaue Kenntnis der Effekte wird helfen, die Zuverlässigkeit und Einsatzdauer von Turbinen zu erhöhen

Enhancement of the priming efficacy of DNA vaccines encoding dendritic cell-targeted antigens by synergistic toll-like receptor ligands

Abstract Background Targeting of protein antigens to dendritic cells (DC) via the DEC205 receptor enhances presentation of antigen-derived peptides on MHC-I and MHC-II molecules and, in the presence of costimulatory signals, antigen-specific immune responses. The immunogenicity and efficacy of DNA vaccination can also be enhanced by fusing the encoded antigen to single chain antibodies directed against DEC205. To further improve this strategy, we evaluated different toll-like receptor ligands (TLR) and CD40 ligands (CD40L) as adjuvants for DNA vaccines encoding a DEC205-single-chain antibody fused to the ovalbumin model antigen or HIV-1 Gag and assessed the priming efficacy of DNA in a DNA prime adenoviral vector boost immunization regimen. Results Mice were primed with the adjuvanted DEC-205 targeted DNA vaccines and boosted with adenoviral vectors encoding the same antigens. CD8+ T cell responses were determined after the adenoviral booster immunization, to determine how well the different DNA immunization regimens prime for the adenoviral boost. In the absence of adjuvants, targeting of DNA-encoded ovalbumin to DCs suppressed CD8+ T-cell responses after the adenoviral booster immunization. CD8+ T-cell responses to the DEC205 targeted DNA vaccines increased only slightly by adding either the TLR-9 ligand CpG, the TLR-3 ligand Poly I:C, or CD40 ligand expression plasmids. However, the combination of both TLR-ligands led to a strong enhancement of CD8+ T-cell responses compared to a non-targeted DNA vaccine. This finding was confirmed using HIV Gag as antigen. Conclusion Although DNA prime adenoviral vector boost immunizations belong to the strongest inducers of cytotoxic T cell responses in different animal models and humans, the CD8+ T cell responses can be further improved by targeting the DNA encoded antigen to DEC205 in the presence of synergistic TLR ligands CpG and Poly I:C

Coherent terabit communications with microresonator Kerr frequency combs

Optical frequency combs enable coherent data transmission on hundreds of wavelength channels and have the potential to revolutionize terabit communications. Generation of Kerr combs in nonlinear integrated microcavities represents a particularly promising option enabling line spacings of tens of GHz, compliant with wavelength-division multiplexing (WDM) grids. However, Kerr combs may exhibit strong phase noise and multiplet spectral lines, and this has made high-speed data transmission impossible up to now. Recent work has shown that systematic adjustment of pump conditions enables low phase-noise Kerr combs with singlet spectral lines. Here we demonstrate that Kerr combs are suited for coherent data transmission with advanced modulation formats that pose stringent requirements on the spectral purity of the optical source. In a first experiment, we encode a data stream of 392 Gbit/s on subsequent lines of a Kerr comb using quadrature phase shift keying (QPSK) and 16-state quadrature amplitude modulation (16QAM). A second experiment shows feedback-stabilization of a Kerr comb and transmission of a 1.44 Tbit/s data stream over a distance of up to 300 km. The results demonstrate that Kerr combs can meet the highly demanding requirements of multi-terabit/s coherent communications and thus offer a solution towards chip-scale terabit/s transceivers

Paleogeography of the Upper Rhine Graben (URG) and the Swiss Molasse Basin (SMB) from Eocene to Pliocene

Twenty paleogeographic maps are presented for Middle Eocene (Lutetian) to Late Pliocene times according to the stratigraphical data given in the companion paper by Berger et al. this volume. Following a first lacustrine-continental sedimentation during the Middle Eocene, two and locally three Rupelian transgressive events were identified with the first corresponding with the Early Rupelian Middle Pechelbronn beds and the second and third with the Late Rupelian ≪ Serie Grise ≫ (Fischschiefer and equivalents). During the Early Rupelian (Middle Pechelbronn beds), a connection between North Sea and URG is clearly demonstrated, but a general connection between North Sea, URG and Paratethys, via the Alpine sea, is proposed, but not proved, during the late Rupelian. Whereas in the southern URG, a major hiatus spans Early Aquitanian to Pliocene times, Early and Middle Miocene marine, brackish and freshwater facies occur in the northern URG and in the Molasse Basin (OMM, OSM); however, no marine connections between these basins could be demonstrated during this time. After the deposition of the molasse series, a very complex drainage pattern developed during the Late Miocene and Pliocene, with a clear connection to the Bresse Graben during the Piacenzian (Sundgau gravels). During the Late Miocene, Pliocene and Quaternary sedimentation persisted in the northern URG with hardly any interruptions. The present drainage pattern of the Rhine river (from Alpine area to the lower Rhine Embayment) was not established before the Early Pleistocen

Comparison of Selective Laser Melted Titanium and Magnesium Implants Coated with PCL

Degradable implant material for bone remodeling that corresponds to the physiological stability of bone has still not been developed. Promising degradable materials with good mechanical properties are magnesium and magnesium alloys. However, excessive gas production due to corrosion can lower the biocompatibility. In the present study we used the polymer coating polycaprolactone (PCL), intended to lower the corrosion rate of magnesium. Additionally, improvement of implant geometry can increase bone remodeling. Porous structures are known to support vessel ingrowth and thus increase osseointegration. With the selective laser melting (SLM) process, defined open porous structures can be created. Recently, highly reactive magnesium has also been processed by SLM. We performed studies with a flat magnesium layer and with porous magnesium implants coated with polymers. The SLM produced magnesium was compared with the titanium alloy TiAl6V4, as titanium is already established for the SLM-process. For testing the biocompatibility, we used primary murine osteoblasts. Results showed a reduced corrosion rate and good biocompatibility of the SLM produced magnesium with PCL coating.DFG/299/11-

SLM produced porous titanium implant improvements for enhanced vascularization and osteoblast seeding

To improve well-known titanium implants, pores can be used for increasing bone formation and close bone-implant interface. Selective Laser Melting (SLM) enables the production of any geometry and was used for implant production with 250-microm pore size. The used pore size supports vessel ingrowth, as bone formation is strongly dependent on fast vascularization. Additionally, proangiogenic factors promote implant vascularization. To functionalize the titanium with proangiogenic factors, polycaprolactone (PCL) coating can be used. The following proangiogenic factors were examined: vascular endothelial growth factor (VEGF), high mobility group box 1 (HMGB1) and chemokine (C-X-C motif) ligand 12 (CXCL12). As different surfaces lead to different cell reactions, titanium and PCL coating were compared. The growing into the porous titanium structure of primary osteoblasts was examined by cross sections. Primary osteoblasts seeded on the different surfaces were compared using Live Cell Imaging (LCI). Cross sections showed cells had proliferated, but not migrated after seven days. Although the cell count was lower on titanium PCL implants in LCI, the cell count and cell spreading area development showed promising results for titanium PCL implants. HMGB1 showed the highest migration capacity for stimulating the endothelial cell line. Future perspective would be the incorporation of HMGB1 into PCL polymer for the realization of a slow factor release.DFG/299/11-

Immunosuppressive therapy influences the accelerated age-dependent T-helper cell differentiation in systemic lupus erythematosus remission patients

Background: CD4+ T cells are of great importance in the pathogenesis of systemic lupus erythematosus (SLE), as an imbalance between CD4+ regulatory T cells (Tregs) and CD4+ responder T cells (Tresps) causes flares of active disease in SLE patients. In this study, we aimed to find the role of aberrant Treg/Tresp cell differentiation for maintaining Treg/Tresp cell balance and Treg functionality. Methods: To determine differences in the differentiation of Tregs/Tresps we calculated the percentages of CD45RA+CD31+ recent thymic emigrant (RTE) Tregs/Tresps and CD45RA+CD31− mature naive (MN) Tregs/Tresps, as well as CD45RA−CD31+ and CD45RA−CD31− memory Tregs/Tresps (CD31+ and CD31− memory Tregs/Tresps) within the total Treg/Tresp pool of 78 SLE remission patients compared with 94 healthy controls of different ages. The proliferation capacity of each Treg/Tresp subset was determined by staining the cells with anti-Ki67 monoclonal antibodies. Differences in the autologous or allogeneic Treg function between SLE remission patients and healthy controls were determined using suppression assays. Results: With age, we found an increased differentiation of RTE Tregs via CD31+ memory Tregs and of RTE Tresps via MN Tresps into CD31− memory Tregs/Tresp in healthy volunteers. This opposite differentiation of RTE Tregs and Tresps was associated with an age-dependent increase in the suppressive activity of both naive and memory Tregs. SLE patients showed similar age-dependent Treg cell differentiation. However, in these patients RTE Tresps differentiated increasingly via CD31+ memory Tresps, whereby CD31− memory Tresps arose that were much more difficult to inhibit for Tregs than those that emerged through differentiation via MN Tresps. Consequently, the increase in the suppressive activity of Tregs with age could not be maintained in SLE patients. Testing the Tregs of healthy volunteers and SLE patients with autologous and nonautologous Tresps revealed that the significantly decreased Treg function in SLE patients was not exclusively attributed to an age-dependent diminished sensitivity of the Tresps for Treg suppression. The immunosuppressive therapy reduced the accelerated age-dependent Tresp cell proliferation to normal levels, but simultaneously inhibited Treg cell proliferation below normal levels. Conclusions: Our data reveal that the currently used immunosuppressive therapy has a favorable effect on the differentiation and proliferation of Tresps but has a rather unfavorable effect on the proliferation of Tregs. Newer substances with more specific effects on the immune system would be desirable