85 research outputs found
Tuberculosis associated thrombocytopenic purpura: effectiveness of antituberculous therapy
Association of immune thrombocytopenic purpura and tuberculosis is a rare condition. In 5 patients presenting with this association, anti-tuberculous therapy was effective on both tuberculosis and thrombocytopenia suggesting a causal relationship between tuberculosis and immune thrombocytopenic purpur
The Transition between Telomerase and ALT Mechanisms in Hodgkin Lymphoma and Its Predictive Value in Clinical Outcomes
International audienceBackground: We analyzed telomere maintenance mechanisms (TMMs) in lymph node samples from HL patients treated with standard therapy. The TMMs correlated with clinical outcomes of patients. Materials and Methods: Lymph node biopsies obtained from 38 HL patients and 24 patients with lymphadenitis were included in this study. Seven HL cell lines were used as in vitro models. Telomerase activity (TA) was assessed by TRAP assay and verified through hTERT immunofluorescence expression; alternative telomere lengthening (ALT) was also assessed, along with EBV status. Results: Both TA and ALT mechanisms were present in HL lymph nodes. Our findings were reproduced in HL cell lines. The highest levels of TA were expressed in CD30â/CD15â cells. Small cells were identified with ALT and TA. Hodgkin and Reed Sternberg cells contained high levels of PML bodies, but had very low hTERT expression. There was a significant correlation between overall survival (p < 10â3), event-free survival (p < 10â4), and freedom from progression (p < 10â3) and the presence of an ALT profile in lymph nodes of EBV+ patients. Conclusion: The presence of both types of TMMs in HL lymph nodes and in HL cell lines has not previously been reported. TMMs correlate with the treatment outcome of EBV+ HL patients
COVID-19 symptoms at hospital admission vary with age and sex: results from the ISARIC prospective multinational observational study
Background:
The ISARIC prospective multinational observational study is the largest cohort of hospitalized patients with COVID-19. We present relationships of age, sex, and nationality to presenting symptoms.
Methods:
International, prospective observational study of 60â109 hospitalized symptomatic patients with laboratory-confirmed COVID-19 recruited from 43 countries between 30 January and 3 August 2020. Logistic regression was performed to evaluate relationships of age and sex to published COVID-19 case definitions and the most commonly reported symptoms.
Results:
âTypicalâ symptoms of fever (69%), cough (68%) and shortness of breath (66%) were the most commonly reported. 92% of patients experienced at least one of these. Prevalence of typical symptoms was greatest in 30- to 60-year-olds (respectively 80, 79, 69%; at least one 95%). They were reported less frequently in children (â€â18 years: 69, 48, 23; 85%), older adults (â„â70 years: 61, 62, 65; 90%), and women (66, 66, 64; 90%; vs. men 71, 70, 67; 93%, each Pâ<â0.001). The most common atypical presentations under 60 years of age were nausea and vomiting and abdominal pain, and over 60 years was confusion. Regression models showed significant differences in symptoms with sex, age and country.
Interpretation:
This international collaboration has allowed us to report reliable symptom data from the largest cohort of patients admitted to hospital with COVID-19. Adults over 60 and children admitted to hospital with COVID-19 are less likely to present with typical symptoms. Nausea and vomiting are common atypical presentations under 30 years. Confusion is a frequent atypical presentation of COVID-19 in adults over 60 years. Women are less likely to experience typical symptoms than men
ETUDE DE PATIENTES DE PHENOTYPE TURNERIEN AVEC PRESENCE DE MATERIEL Y (A PROPOS DE 7 CAS (DES BIOL. MED.))
PARIS7-Xavier Bichat (751182101) / SudocPARIS-BIUM (751062103) / SudocSudocFranceF
Syndromes lymphoprolifératifs et immunosuppresseurs
Les syndromes lymphoprolifĂ©ratifs compliquant lâĂ©volution de pathologies liĂ©es Ă un dĂ©ficit immunitaire, quâil soit primitif ou acquis, sont des complications bien identifiĂ©es et dâĂ©volution pĂ©jorative. Parmi les causes de dĂ©ficit immunitaire acquis, les traitements immunomodulateurs dans le cadre de pathologies auto-immunes doivent faire lâobjet dâobservatoires afin de dĂ©terminer le rĂŽle respectif des immunosuppresseurs et de la pathologie sous-jacente caractĂ©risĂ©e par une stimulation lymphocytaire persistante et un dĂ©sĂ©quilibre cytokinique. Les syndromes lymphoprolifĂ©ratifs survenant dans le contexte des greffes dâorgane ou de moelle osseuse sont des complications des traitements immunosuppresseurs et probablement de la stimulation antigĂ©nique sous-jacente. Leur frĂ©quence varie selon les traitements immunosuppresseurs utilisĂ©s, le type dâorgane greffĂ© et le statut de lâinfection par le virus dâEpstein-Barr (EBV) du receveur. Ces prolifĂ©rations tumorales dĂ©veloppĂ©es aux dĂ©pens des cellules lymphoĂŻdes du receveur, dĂ©rivant de cellules du centre germinatif ou post-centre germinatif, rĂ©sultent de diffĂ©rents mĂ©canismes de lymphomagenĂšse. Le rĂŽle de lâEBV confĂ©rant un avantage prolifĂ©ratif aux cellules lymphoĂŻdes dans un contexte de dĂ©ficit immunitaire est majeur dans les lĂ©sions prĂ©coces et polymorphes. Ce mĂ©canisme physiopathogĂ©nique justifie la surveillance de la charge virale du sang pĂ©riphĂ©rique et de la rĂ©ponse immune anti-EBV chez les patients transplantĂ©s. Les anomalies gĂ©nĂ©tiques plus frĂ©quentes dans les prolifĂ©rations lymphomateuses monomorphes et tardives correspondent soit Ă des pertes dâhĂ©tĂ©rozygotie, soit Ă des dĂ©sĂ©quilibres chromosomiques, soit Ă des mutations aberrantes, soit Ă des phĂ©nomĂšnes dâinstabilitĂ© des microsatellites aboutissant Ă des mutations affectant des gĂšnes impliquĂ©s dans des phĂ©nomĂšnes dâapoptose ou de recombinaison de lâADN. La connaissance des mĂ©canismes de lymphomagenĂšse chez ces patients immunodĂ©ficients permet une approche de dĂ©pistage par le suivi de marqueurs viraux et immunologiques et une attitude thĂ©rapeutique ciblĂ©e
Lung Fibroblasts from Idiopathic Pulmonary Fibrosis Patients Harbor Short and Unstable Telomeres Leading to Chromosomal Instability
Idiopathic pulmonary fibrosis (IPF) is associated with several hallmarks of aging including telomere shortening, which can result from germline mutations in telomere related genes (TRGs). Here, we assessed the length and stability of telomeres as well as the integrity of chromosomes in primary lung fibroblasts from 13 IPF patients (including seven patients with pathogenic variants in TRGs) and seven controls. Automatized high-throughput detection of telomeric FISH signals highlighted lower signal intensity in lung fibroblasts from IPF patients, suggesting a telomere length defect in these cells. The increased detection of telomere loss and terminal deletion in IPF cells, particularly in TRG-mutated cells (IPF-TRG), supports the notion that these cells have unstable telomeres. Furthermore, fibroblasts from IPF patients with TRGs mutations exhibited dicentric chromosomes and anaphase bridges. Collectively, our study indicates that fibroblasts from IPF patients exhibit telomere and chromosome instability that likely contribute to the physiopathology
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