Therapeutic potential of essential oil of Melaleuca quinquenervia (Myrtaceae) in a rat model of ethanol-induced peptic ulcer

Purpose: To evaluate the effects of M. quinquenervia extract on ethanol-induced peptic ulcer in rats. Methods: The following three groups of (n = 6) Sprague Dawley rats were included in this study: vehicle (C), ethanol-administered (E) and ethanol + M. quinquenervia-treated (MQ). MQ group rats received 100 μg/mL M. quinquenervia essential oil just before 96 % ethanol induction (1 ml/kg). One hour after ulcer induction, the animals were euthanized, and gastric and duodenal tissues were removed. Tissue samples were analysed for myeloperoxidase (MPO) activity, malondialdehyde (MDA), glutathione (GSH), myeloperoxidase (MPO) activity, malondialdehyde (MDA) and glutathione (GSH) levels, and histopathological examinations were performed by light microscopy. Results: Gastric and duodenal GSH levels that decreased in the ethanol-administered ulcer groups (p < 0.001), rose following MQ treatment (p < 0.5). Moreover, elevated MPO and MDA levels (p < 0.5) in gastric tissues decreased after MQ-treatment. Similarly, the MQ-treated group showed recovery and control-like morphology compared to the ethanol group in both gastric and duodenal tissues when examined by microscopy. Conclusion: The results indicate that M. quinquenervia extract has a positive impact on gastric injury in rats due to its antioxidant activity. Thus, the plant has a potential for the clinical management of gastric ulcer

Thermo-mechanical behavior of titanium beryllide pebble beds at elevated temperatures

The thermomechanical behavior of titanium beryllide pebble beds was investigated experimentally at temperatures between 200 and 500°C in helium atmosphere at atmospheric pressure. The pebbles consist of a mixture of TiBe12 and Ti2Be17 titanate beryllide phases and a small residual amount of Be phase, denominated as Be-7.7Ti. Like previous experiments at ambient temperature [1], the pebble beds were compressed uniaxially up to 4.5MPa and the effective thermal conductivity k was measured using the hot wire technique. Compared to ambient temperature, the stress-strain curves do not differ significantly in investigated temperature range. Because the thermal conductivity of solid TiBe12 is fairly constant in a wide temperature range [2], k increases moderately with increasing temperature because of the increasing thermal conductivity of helium. Compared to beryllium pebble beds, the k of the Be-7.7Ti pebble beds increases again much lesser because of the significantly smaller thermal conductivity of the solid material and the mechanically harder behavior resulting in smaller contact surfaces

A comparative in vitro evaluation of two different magnetic devices detecting the stability of osseo-integrated implants

Geckili O, Bilhan H, Cilingir A, Mumcu E, Bural C. A comparative in vitro evaluation of two different magnetic devices detecting the stability of osseo-integrated implants. J Periodont Res 2012; 47: 508513. (c) 2012 John Wiley & Sons A/S Background and Objective: It is unknown whether the resonance frequency analysis (RFA) measurements made by two different magnetic resonance frequency analysers are comparable. This in vitro study was designed to compare the RFA measurements made by the two magnetic resonance frequency analysers and to evaluate the intra- and interobserver reliability of the magnetic devices. Material and Methods: Thirty-two implants were placed in four cow ribs. The RFA value of each implant was measured by five different examiners. The measurements were repeated five times, in both the buccal and mesial directions, for each implant at 2 h intervals, and the averages of registered implant stability quotient (ISQ) units were recorded as the buccal ISQ value and the mesial ISQ value for every implant. Results: No statistically significant differences (p > 0.05) were observed between the RFA measurements made by the two magnetic devices. The intra-observer reliability of both devices was excellent, whereas the interobserver reliability of the devices was poor. Conclusion: The results of the RFA measurements of both tested devices overlap. Although both devices show excellent intra-observer reliability, there are variations between the measurements of different examiners

Curcumin induces DNA damage by mediating homologous recombination mechanism in triple negative breast cancer

PMID = 3157335

Синтез, транспортные и ионофорные свойства БИС-а аминометилфосфиноксидов и фосфорилированных азаподантов: автореферат диссертации на соискание ученой степени к.х.н.: специальность 02.00.08

Curcumin has a therapeutic potential activity through modulation of different signaling pathways in various types of cancer. However, the relationship between the efficacy of curcumin and the homologous recombination (HR) mechanism which plays important roles in the repair of double strand DNA (dsDNA) breaks remains uncertain. Herein, we explored curcumin-dependent dsDNA breaks and the association of curcumin with HR mechanism in triple negative breast cancer (TNBC). The cytotoxic and therapeutic activity of curcumin on HCC1937 (BRCA1 mutant), MDA-MB-231 (BRCA1 wild type) TNBC and HUVEC control cell lines were assessed. Then, the expression level and subcellular localization of H2AX, PARP1, BRCA1 and RAD51 were detected by RT-PCR and immunofluorescence analysis. Furthermore, ultrastructural changes of cell death were observed by TEM. Our findings for the first time demonstrated that curcumin?s therapeutic activity was more pronounced in HCC1937 cells through the suppression of HR mechanism and the induction of dsDNA breaks. Consequently, curcumin based therapy could benefit in patients with TNBC particularly especially in women with a BRCA1 mutation

Two-body wear of occlusal splint materials

This study investigates the wear resistance of four different types of occlusal splint materials based on two-body wear simulations under wet and dry conditions. Twenty specimens of each splint material (Dentalon Plus, Orthoplast, Biocryl C, and Eclipse), each with a diameter of 16 mm and a thickness of 3 mm, were tested, half under wet and half under dry conditions. Each wear test was performed using a device called chewing simulator CS-4 (n = 10; test load: 50 N; number of cycles: 10 000, 20 000, and 30 000; continuous rinsing with 30 degrees C water for wet conditions); the antagonists were simulated using steel balls. Wear was determined using a 3D laser scanner and a surface analysis program. To detect significant statistical differences, wear data after 10 000; 20 000; and 30 000 cycles were compared using the KruskalWallis test and the MannWhitney U-test. The level of significance was set at 5%. Significant differences were found between the groups of different materials tested under wet conditions (P 0.05). No significant difference was found between the wet and dry conditions for all materials and cycles (P > 0.05). For groups of different materials tested under wet conditions, the degree of volume loss generated in the Chewing Simulator CS-4 was found to differ significantly for different numbers of cycles. The presence of water had no effect on the volume loss in the different material groups that were tested