Potential for Farmers’ Cooperatives to Convert Coffee Husks into Biochar and Promote the Bioeconomy in the North Ecuadorian Amazon

Improving the livelihoods of communities living in fragile ecosystems, such as tropical forests, is among the main strategies to promote their conservation and preserve wildlife. In the Ecuadorian Amazon, farmers’ cooperatives are recognized as an important mechanism to improve the socioeconomic conditions of local communities. This study analyzes the integration of pyrolysis processes to convert agricultural waste into biochar as a way to implement the bioeconomy in these organizations. We found that post-harvesting processes in the studied farmers’ cooperatives are similar, and coffee husks are a potential feedstock to produce biochar. Although the environmental policies in Ecuador consider the valorization of agricultural waste, we did not find any specific standard to regulate the operation of pyrolysis facilities. Nonetheless, conversion of agricultural waste into biochar can contribute to (i) replacement of subsidized fossil fuels used in drying processes, (ii) prevention of environmental pollution caused by accumulation of waste, (iii) emergence of new income sources linked with the provision of carbon sequestration services, and (iv) the long-term maintenance of soil fertility. Currently, demonstration projects are needed to stimulate collaboration among farmers’ cooperatives, academia, the government, international cooperation agencies, and existing forest conservation initiatives.Fundação para a Ciência e a TecnologiaBundesministerium für Bildung und ForschungPeer Reviewe

Checklist of macro-invertebrates of the special conservation area “Poggi di prata”(Grosseto, Central Italy) through a citizen-science and expert-based approach

The assessment of species composition in a certain area may become outdated over time due to community dynamics including species range expansion, but also to local extinctions, species introductions and taxonomic redefinition. Therefore, updated checklists are required for animal conservation and management. Exhaustive checklists of invertebrate species may be challenging, as species determination often requires the analysis by specialists, but they are fundamental for local conservation practices. In this work, we provided an annotated preliminary checklist of invertebrates of the Special Conservation Area “Poggi di Prata” (province of Grosseto, southern Tuscany), detected through field samplings with experts, and a permanent Bioblitz set out on an online citizen-science platform (iNaturalist.org). The final dataset (1898-2020) included 329 records of 282 species (217 insects, 34 gastropods, 30 arachnids and 1 chilopod). Most records were uploaded on iNaturalist (about 56.5%), others came from observations or sampling collections (37%) and were determined by specialists. Only the remaining 6.5% of records came from published studies. Three species were protected by the Habitat Directive, 15 by the Tuscan Regional Law. We also detected two endemic or near-endemic taxa of this area: the beetle Paramaurops diecki massetanus and the land snail Marmorana saxetana. The unexpected (Italian southernmost) record of Gaurotes virginea needs to be deepened. Furthermore, 12 alien species, including insects affecting human economy and wellness (e.g., Rhinchophorous ferrugineus, Aedes albopictus, Halyomorpha halys, Dryocosmus kuriphilus and Cydalima perspectalis), were also detected. With our work, we confirmed that citizen-science platforms (e.g. iNaturalist) are valuable tools, complementary to field-work by specialists, to map local biodiversity and they may help to improve biogeographical knowledge

Interactions between BRD4S, LOXL2, and MED1 drive cell cycle transcription in triple‐negative breast cancer

Cell cycle; Gene expression; Triple-negative breast cancerCiclo celular; Expresión génica; Cáncer de mama triple negativoCicle cel·lular; Expressió gènica; Càncer de mama triple negatiuTriple‐negative breast cancer (TNBC) often develops resistance to single‐agent treatment, which can be circumvented using targeted combinatorial approaches. Here, we demonstrate that the simultaneous inhibition of LOXL2 and BRD4 synergistically limits TNBC proliferation in vitro and in vivo. Mechanistically, LOXL2 interacts in the nucleus with the short isoform of BRD4 (BRD4S), MED1, and the cell cycle transcriptional regulator B‐MyB. These interactions sustain the formation of BRD4 and MED1 nuclear transcriptional foci and control cell cycle progression at the gene expression level. The pharmacological co‐inhibition of LOXL2 and BRD4 reduces BRD4 nuclear foci, BRD4‐MED1 colocalization, and the transcription of cell cycle genes, thus suppressing TNBC cell proliferation. Targeting the interaction between BRD4S and LOXL2 could be a starting point for the development of new anticancer strategies for the treatment of TNBC.We thank the CRG genomics unit, the CRG‐UPF flow cytometry unit, and the VHIO mouse facility for their contribution. We thank Pharmaxis for the supply of PXS LOXL2 inhibitors. SS is supported by the Plan Estatal de I + D + I (COMBAT PID2019‐110598GA‐I00), and the ERC Starting Grant (ERC‐StG‐852343‐EPICAMENTE). LP‐R is supported by the Juan de la Cierva‐Formación fellowship (FJC2019‐040598‐I) and Fundación Franscico Cobos fellowship. TVT is supported by Plan Estatal de I + D + I (PID2019‐108008RJ‐I00), AECC (INVES20036TIAN), and a Ramón y Cajal investigator contract (RYC2020‐029098‐I). DC is supported by the la Caixa Foundation PhD fellowship (ID 100010434; fellowship code LCF/BQ/DI19/11730061)

FIRST RECORD OF RICANIA SPECULUM(WALKER, 1851) (HEMIPTERA RICANIIDAE)FROM VENETO, PIEDMONT AND LATIUM REGIONS AND NEW HOST PLANTS

The distribution of Ricania speculum(Walker, 1851) (Hemiptera Fulgoromorpha Ricaniidae) in Italy was updatedand the species was recorded for the first time in Veneto (2017), Piedmont (2018) and Latium (2018) regions. Theoccurrence of this species in Sardinia, previously recorded, is here excluded. Moreover, 33 new host plants belonging to 29families resulted to be attacked by this alien invasive species confirming its outstanding polyphagy

COVID-19 vaccination in patients receiving allergen immunotherapy (AIT) or biologicals:EAACI recommendations

Immune modulation is a key therapeutic approach for allergic diseases, asthma and autoimmunity. It can be achieved in an antigen-specific manner via allergen immunotherapy (AIT) or in an endotype-driven approach using biologicals that target the major pathways of the type 2 (T2) immune response: immunoglobulin (Ig)E, interleukin (IL)-5 and IL-4/IL-13 or non-type 2 response: anti-cytokine antibodies and B-cell depletion via anti-CD20. Coronavirus disease 2019 (COVID-19) vaccination provides an excellent opportunity to tackle the global pandemics and is currently being applied in an accelerated rhythm worldwide. The vaccine exerts its effects through immune modulation, induces and amplifies the response against the severe acute respiratory syndrome coronavirus (SARS-CoV-2). Thus, as there may be a discernible interference between these treatment modalities, recommendations on how they should be applied in sequence are expected. The European Academy of Allergy and Clinical Immunology (EAACI) assembled an expert panel under its Research and Outreach Committee (ROC). This expert panel evaluated the evidence and have formulated recommendations on the administration of COVID-19 vaccine in patients with allergic diseases and asthma receiving AIT or biologicals. The panel also formulated recommendations for COVID-19 vaccine in association with biologicals targeting the type 1 or type 3 immune response. In formulating recommendations, the panel evaluated the mechanisms of COVID-19 infection, of COVID-19 vaccine, of AIT and of biologicals and considered the data published for other anti-infectious vaccines administered concurrently with AIT or biologicals

Subspecific taxonomy of African porcupines Hystrix spp.: is there anything beyond the species level?

Taxonomy is a mistreated matter, but its role in ecology, behaviour and conservation studies is pivotal. Disentangling amongst different subspecies is challenging given the high arbitrariness level in determining thresholds of genetic and morphological distances. Splitting frenzy trends have increased the number of animal taxa and for most of them a critical redefinition is required. In this work, we reviewed knowledge and validity of subspecific taxa identified for African crested porcupines Hystrix cristata and Hystrix africaeaustralis. In the past, several subspecies were recognized for both the species, but successive works suggested H. cristata and H. africaeaustralis as monotypic species with no clear explanation. Recently, the validity of the taxon H. cristata senegalica has been claimed again. We analysed all available data and discussed all the subspecific taxa in light of both genetic and morphological data. We revalidated here the synonymy Hystrix senegalica Cuvier, 1823 = Hystrix cristata Linnaeus, 1758. Syn. rev. Two names are treated as nomina dubia: Acanthion daubentonii Cuvier, 1823 (formalization) and "Hystrix capensis Gr.". Hystrix cristata var. alba de Selys-Longchamps, 1839 has been deleted from the synonymic list ofH. cristata. Neither mitochondrial nor nuclear DNA data militate for the existence of any subspecific taxon, although further data are required for H. cristata from East Africa (e.g., Kenya and Tanzania). Similarly, morphology seems to play for a clinal variation in both species. For available data, we thus strongly recommend to keep both H. cristata and H. africaeaustralis as monotypic species

Increased blood glucose variability during therapeutic hypothermia and outcome after cardiac arrest.

Hypothermia impairs blood glucose homeostasis and insulin sensitivity. However, the impact of therapeutic hypothermia on blood glucose levels and insulin requirements is unknown. We analyzed blood glucose variability during therapeutic hypothermia in patients with coma after cardiac arrest and examined its impact on outcome.Journal ArticleSCOPUS: ar.jinfo:eu-repo/semantics/publishe

Interactions between BRD4S, LOXL2, and MED1 drive cell cycle transcription in triple-negative breast cancer

Triple-negative breast cancer (TNBC) often develops resistance to single-agent treatment, which can be circumvented using targeted combinatorial approaches. Here, we demonstrate that the simultaneous inhibition of LOXL2 and BRD4 synergistically limits TNBC proliferation in vitro and in vivo. Mechanistically, LOXL2 interacts in the nucleus with the short isoform of BRD4 (BRD4S), MED1, and the cell cycle transcriptional regulator B-MyB. These interactions sustain the formation of BRD4 and MED1 nuclear transcriptional foci and control cell cycle progression at the gene expression level. The pharmacological co-inhibition of LOXL2 and BRD4 reduces BRD4 nuclear foci, BRD4-MED1 colocalization, and the transcription of cell cycle genes, thus suppressing TNBC cell proliferation. Targeting the interaction between BRD4S and LOXL2 could be a starting point for the development of new anticancer strategies for the treatment of TNBC.We thank Pharmaxis for the supply of PXS LOXL2 inhibitors. SS is supported by the Plan Estatal de I + D + I (COMBAT PID2019‐110598GA‐I00), and the ERC Starting Grant (ERC‐StG‐852343‐EPICAMENTE). LP‐R is supported by the Juan de la Cierva‐Formación fellowship (FJC2019‐040598‐I) and Fundación Franscico Cobos fellowship. TVT is supported by Plan Estatal de I + D + I (PID2019‐108008RJ‐I00), AECC (INVES20036TIAN), and a Ramón y Cajal investigator contract (RYC2020‐029098‐I). DC is supported by the la Caixa Foundation PhD fellowship (ID 100010434; fellowship code LCF/BQ/DI19/11730061)