SPECT Imaging of Pulmonary Blood Flow in a Rat

Small animal imaging is experiencing rapid development due to its importance in providing high-throughput phenotypic data for functional genomics studies. We have developed a single photon emission computed tomography (SPECT) system to image the pulmonary perfusion distribution in the rat. A standard gamma camera, equipped with a pinhole collimator, was used to acquire SPECT projection images at 40 sec/view of the rat thorax following injection of Tc99m labeled albumin that accumulated in the rat\u27s lungs. A voxel-driven, ordered-subset expectation maximization reconstruction was implemented. Following SPECT imaging, the rat was imaged using micro-CT with Feldkamp conebeam reconstruction. The two reconstructed image volumes were fused to provide a structure/function image of the rat thorax. Reconstruction accuracy and performance were evaluated using numerical simulations and actual imaging of an experimental phantom consisting of Tc99m filled chambers with known diameters and count rates. Full-width half-maximum diameter measurement errors decreased with increasing chamber diameter, ranging from \u3c 6% down to 0.1%. Errors in the ratio of count rate estimates between tubes were also diameter dependent but still relatively small. This preliminary study suggests that SPECT will be useful for imaging and quantifying the pulmonary blood flow distribution and the distribution of Tc99m labeled ligands in the lungs of small laboratory animals

Inequity in access to transplantation in the UK

Background and objectives Despite the presence of a universal health care system, it is unclear if there is intercenter variation in access to kidney transplantation in the United Kingdom. This study aims to assess whether equity exists in access to kidney transplantation in the United Kingdom after adjustment for patient-specific factors and center practice patterns. Design, setting, participants, & measurements In this prospective, observational cohort study including all 71 United Kingdom kidney centers, incident RRT patients recruited between November 2011 and March 2013 as part of the Access to Transplantation and Transplant Outcome Measures study were analyzed to assess preemptive listing (n=2676) and listing within 2 years of starting dialysis (n=1970) by center. Results Seven hundred and six participants (26%) were listed preemptively, whereas 585 (30%) were listed within 2 years of commencing dialysis. The interquartile range across centers was 6%–33% for preemptive listing and 25%–40% for listing after starting dialysis. Patient factors, including increasing age, most comorbidities, body mass index >35 kg/m2, and lower socioeconomic status, were associated with a lower likelihood of being listed and accounted for 89% and 97% of measured intercenter variation for preemptive listing and listing within 2 years of starting dialysis, respectively. Asian (odds ratio, 0.49; 95% confidence interval, 0.33 to 0.72) and Black (odds ratio, 0.43; 95% confidence interval, 0.26 to 0.71) participants were both associated with reduced access to preemptive listing; however Asian participants were associated with a higher likelihood of being listed after starting dialysis (odds ratio, 1.42; 95% confidence interval, 1.12 to 1.79). As for center factors, being registered at a transplanting center (odds ratio, 3.1; 95% confidence interval, 2.36 to 4.07) and a universal approach to discussing transplantation (odds ratio, 1.4; 95% confidence interval, 1.08 to 1.78) were associated with higher preemptive listing, whereas using a written protocol was associated negatively with listing within 2 years of starting dialysis (odds ratio, 0.7; 95% confidence interval, 0.58 to 0.9). Conclusions Patient case mix accounts for most of the intercenter variation seen in access to transplantation in the United Kingdom, with practice patterns also contributing some variation. Socioeconomic inequity exists despite having a universal health care system

Methanol on Enceladus

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/95284/1/grl26203.pd

Occurrence of Compact Groups of Galaxies through Cosmic Time

We use the outputs of a semianalytical model of galaxy formation run on the Millennium Simulation to investigate the prevalence of 3D compact groups (CGs) of galaxies from z = 11 to 0. Our publicly available code identifies CGs using the 3D galaxy number density, the mass ratio of secondary+tertiary to the primary member, mass density in a surrounding shell, the relative velocities of candidate CG members, and a minimum CG membership of three. We adopt "default" values for the first three criteria, representing the observed population of Hickson CGs at z = 0. The percentage of nondwarf galaxies (M > 5 × 10^8 h^(−1) M ⊙) in CGs peaks near z ~ 2 for the default set and in the range of z ~ 1–3 for other parameter sets. This percentage declines rapidly at higher redshifts (z ≳ 4), consistent with the galaxy population as a whole being dominated by low-mass galaxies excluded from this analysis. According to the most liberal criteria, ≾3% of nondwarf galaxies are members of CGs at the redshift where the CG population peaks. Our default criteria result in a population of CGs at z < 0.03 with number densities and sizes consistent with Hickson CGs. Tracking identified CG galaxies and merger products to z = 0, we find that ≾16% of nondwarf galaxies have been CG members at some point in their history. Intriguingly, the great majority (96%) of z = 2 CGs have merged to a single galaxy by z = 0. There is a discrepancy in the velocity dispersions of Millennium Simulation CGs compared to those in observed CGs, which remains unresolved

The Occurrence of Compact Groups of Galaxies Through Cosmic Time

We use the outputs of a semi-analytical model of galaxy formation run on the Millennium Simulation to investigate the prevalence of 3D compact groups (CGs) of galaxies from $z = 11$ to 0. Our publicly available code identifies CGs using the 3D galaxy number density, the mass ratio of secondary+tertiary to the primary member, mass density in a surrounding shell, the relative velocities of candidate CG members, and a minimum CG membership of three. We adopt "default" values for the first three criteria, representing the observed population of Hickson CGs at $z = 0$. The percentage of non-dwarf galaxies ($M > 5 \times 10^{8}h^{-1}\ M_{\odot}$) in CGs peaks near $z \sim 2$ for the default set, and between $z \sim 1 - 3$ for other parameter sets. This percentage declines rapidly at higher redshifts ($z \gtrsim 4$), consistent with the galaxy population as a whole being dominated by low-mass galaxies excluded from this analysis. According to the most liberal criteria, $\lesssim 3\%$ of non-dwarf galaxies are members of CGs at the redshift where the CG population peaks. Our default criteria result in a population of CGs at $z < 0.03$ with number densities and sizes consistent with Hickson CGs. Tracking identified CG galaxies and merger products to $z = 0$, we find that $\lesssim 16\%$ of non-dwarf galaxies have been CG members at some point in their history. Intriguingly, the great majority ($96\%$) of $z = 2$ CGs have merged to a single galaxy by $z= 0$. There is a discrepancy in the velocity dispersions of Millennium Simulation CGs compared to those in observed CGs, which remains unresolved.Comment: Revised version to match published version. Uses likeapj.cls (v1.1.5), likeapj.bst style files, 11 pages, 7 figures, 1 table. Compact group detection code available at https://github.com/cdw9bf/CompactGroup ; LaTex style files available at https://github.com/qtast/likeapj/releases/lates

An ultrasensitive reverse transcription polymerase chain reaction assay to detect asymptomatic low-density Plasmodium falciparum and Plasmodium vivax infections in small volume blood samples.

BackgroundHighly sensitive, scalable diagnostic methods are needed to guide malaria elimination interventions. While traditional microscopy and rapid diagnostic tests (RDTs) are suitable for the diagnosis of symptomatic malaria infection, more sensitive tests are needed to screen for low-density, asymptomatic infections that are targeted by interventions aiming to eliminate the entire reservoir of malaria infection in humans.MethodsA reverse transcription polymerase chain reaction (RT- PCR) was developed for multiplexed detection of the 18S ribosomal RNA gene and ribosomal RNA of Plasmodium falciparum and Plasmodium vivax. Simulated field samples stored for 14 days with sample preservation buffer were used to assess the analytical sensitivity and specificity. Additionally, 1750 field samples from Southeastern Myanmar were tested both by RDT and ultrasensitive RT-PCR.ResultsLimits of detection (LoD) were determined under simulated field conditions. When 0.3 mL blood samples were stored for 14 days at 28 °C and 80% humidity, the LoD was less than 16 parasites/mL for P. falciparum and 19.7 copies/µL for P. vivax (using a plasmid surrogate), about 10,000-fold lower than RDTs. Of the 1739 samples successfully evaluated by both ultrasensitive RT-PCR and RDT, only two were RDT positive while 24 were positive for P. falciparum, 108 were positive for P. vivax, and 127 were positive for either P. vivax and/or P. falciparum using ultrasensitive RT-PCR.ConclusionsThis ultrasensitive RT-PCR method is a robust, field-tested screening method that is vastly more sensitive than RDTs. Further optimization may result in a truly scalable tool suitable for widespread surveillance of low-level asymptomatic P. falciparum and P. vivax parasitaemia

Extinction Corrected Star Formation Rates Empirically Derived from Ultraviolet-Optical Colors

Using a sample of galaxies from the Sloan Digital Sky Survey spectroscopic catalog with measured star-formation rates (SFRs) and ultraviolet (UV) photometry from the GALEX Medium Imaging Survey, we derived empirical linear correlations between the SFR to UV luminosity ratio and the UV-optical colors of blue sequence galaxies. The relations provide a simple prescription to correct UV data for dust attenuation that best reconciles the SFRs derived from UV and emission line data. The method breaks down for the red sequence population as well as for very blue galaxies such as the local ``supercompact'' UV luminous galaxies and the majority of high redshift Lyman Break Galaxies which form a low attenuation sequence of their own.Comment: 20 pages, 11 figures, accepted for publication in the ApJS GALEX special issu

Bis(μ2-η2:η2-2,4,6-trimethyl­benzonitrile)­bis­[(N-isopropyl-3,5-dimethyl­anilido)molybdenum(III)](Mo—Mo)

The title compound, [Mo2(C11H16N)4(C10H11N)2], is a dinuclear molybdenum complex with a formal metal–metal bond [Mo⋯Mo separation = 2.5946 (8) Å], four anilide-type ligands and two bridging mesityl nitrile groups. There are two inversion symmetric mol­ecules in the unit cell (an inversion center is localized at the mid-point of the Mo—Mo bond), each with approximate non-crystallographic C 2h symmetry. The mol­ecules contain disordered isopropyl and 3,5-C6H3Me2 groups on different anilido ligands; the major component having an occupancy of 0.683 (7). The complex was obtained in low yield as the product from the reaction between the bridging pyrazine adduct of molybdenum tris­-anilide ([μ2-(C4H4N2){Mo(C11H16N)3}2]) and mesityl nitrile with a loss of one anilido ligand