Assessment of Altimetry Using Ground-Based GPS Data from the 88S Traverse, Antarctica, in Support of ICESat-2

We conducted a 750 km kinematic GPS survey, referred to as the 88S Traverse, based out of South Pole Station, Antarctica, between December 2017 and January 2018. This ground-based survey was designed to validate space-borne altimetry and airborne altimetry developed at NASA. The 88S Traverse intersects 20% of the ICESat-2 satellite orbits on a route that has been flown by two different Operation IceBridge airborne laser altimeters: the Airborne Topographic Mapper (ATM; 26 October 2014) and the University of Alaska Fairbanks (UAF) Lidar (30 November and 3 December 2017). Here we present an overview of the ground-based GPS data quality and a quantitative assessment of the airborne laser altimetry over a flat section of the ice sheet interior. Results indicate that the GPS data are internally consistent (1:1+- 4:1 cm). Relative to the ground-based 88S Traverse data, the elevation biases for ATM and the UAF lidar range from -9:5 to 3.6 cm, while surface measurement precisions are equal to or better than 14.1 cm. These results suggest that the ground-based GPS data and airborne altimetry data are appropriate for the validation of ICESat-2 surface elevation data

Four different emissions from a Pt(Bodipy)(PEt3)(2)(S-Pyrene) dyad

The Pt(bodipy)-(mercaptopyrene) dyad BPtSPyr shows four different emissions: intense near-infrared phosphorescence (Φph up to 15%) from a charge-transfer state pyrS˙+-Pt-BDP˙−, additional fluorescence and phosphorescence emissions from the 1ππ* and 3ππ* states of the bodipy ligand at r.t., and phosphorescence from the pyrene 3ππ* and the bodipy 3ππ* states in a glassy matrix at 77 K.publishe

An undernutrition screening score for dogs with protein‐losing enteropathy: A prospective multicenter study

BACKGROUND: The impact of undernutrition in dogs with protein-losing enteropathy (PLE) caused by inflammatory enteritis, intestinal lymphangiectasia, or both and which variables are most predictive of outcome are unknown. OBJECTIVES: Develop an undernutrition screening score (USS) for use at the time of diagnosis of PLE in dogs, which is predictive of outcome. ANIMALS: Fifty-seven dogs with PLE prospectively recruited from 3 referral hospitals in the United Kingdom. METHODS: An USS based on the presence and severity of 5 variables: appetite, weight loss, and body, muscle, and coat condition and scored out of 15, with higher scores reflecting worse undernutrition, was calculated at the time of diagnosis. Follow-up information was obtained for at least 6 months. RESULTS: Dogs that failed to achieve clinical remission within 6 months had higher USS at diagnosis compared with dogs that achieved remission (median, 7.5; range, 2-14 and median, 5; range, 0-14, respectively). The USS at diagnosis gave an area under the receiver operating characteristic curve (AUC) of 0.656 for predicting nonclinical remission within 6 months, whereas a score consisting of just epaxial muscle loss and coat condition resulted in a larger AUC of 0.728. CONCLUSIONS AND CLINICAL IMPORTANCE: Of the 5 variables assessed in the USS, a combination of epaxial muscle loss and coat condition was most predictive of not achieving clinical remission within 6 months in dogs with PLE. Additional studies will help determine the effect of changes in USS and the 5 associated variables after diagnosis on outcome variables in these dogs

Directing energy transfer in Pt(bodipy)(mercaptopyrene) dyads

We report on the photophysical properties of three dyads that combine a 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene (bodipy, BDP) and a mercaptopyrene (SPyr) dye ligand at a Pt(PEt3)(2) fragment. sigma-Bonding of the dyes to the Pt ion promotes intersystem crossing (ISC) via the external heavy atom effect. The coupling of efficient ISC with charge-transfer from the electron-rich mercaptopyrene to the electron-accepting BDP ligand (PB-CT) gives rise to a multitude of (potentially) emissive states. This culminates in the presence of four different emissions for the mono- and dinuclear complexes BPtSPyr and BPtSPyrSPtB with an unsubstituted BDP ligand and either a terminal 1-mercaptopyrene or a bridging pyrene-1,6-dithiolate ligand. Thus, in fluid solution, near IR emission at 724 nm from the (PB)-P-3-CT state is observed with a quantum yield of up to 15%. Excitation into the BDP-based (1)pi pi* or the pyrene-based (1)pi pi* band additionally trigger fluorescence and phosphorescence emissions from the BDP-centred (1)pi pi* and (3)pi pi* states. In frozen solution, at 77 K, phosphorescence from the pyrene ligand becomes the prominent emission channel, while PB-CT emission is absent. Alkylation of the BDP ligand in KBPtSPyr funnels all excitation energy into fluorescence and phosphorescence emissions from the KBDP ligand. The assignments of the various excited states and the deactivation cascades were probed by absorption and emission spectroscopy, transient absorption spectroscopy, electrochemical and UV/Vis/NIR spectroelectrochemical measurements, and by quantum chemical calculations. Our conclusions are further corroborated with the aid of suitable reference compounds comprising of just one chromophore. All dyads are triplet sensitizers and are able to generate singlet oxygen

Sensory Impact of Extended Maceration and Regulated Deficit Irrigation on Washington State Cabernet Sauvignon Wines

Irrigation practices such as regulated deficit irrigation (RDI) and winemaking practices such as extended maceration have been experimentally evaluated from a chemical perspective but their impacts on sensory composition and interactive effects merit scientific attention. This study evaluated the sensory impact of extended maceration applied to Cabernet Sauvignon grapes sourced from a vineyard subjected to four RDI treatments: replenishment of 100%, 70%, and 25% of full-vine crop evapotranspiration (ETc) from fruit set until veraison followed by 100% ETc until harvest (labeled 100% ETc, 70% ETc, and 25% ETc, respectively) and 25% ETc from fruit set to veraison followed by 100% ETc from veraison to harvest (labeled 25/100% ETc). Each RDI treatment was replicated four times (n = 4) and made into wine, with two replicates designated as controls (10 day skin contact) and two as extended maceration (30 day skin contact). Wines were evaluated by descriptive analysis with a trained panel (n = 15) and chemical and sensory data were correlated using canonical correlation analysis. Wine-perceived saturation and purple component ratings were highest in 25% ETc wines and were highly correlated with the concentration of flavonols, malvidin- and delphinidin-derivatives, and small polymeric pigments. Fruit-based aroma descriptors were highest in the 25/100% ETc and 70% ETc wines. Extended maceration increased perceived astringency and bitterness, which were in turn correlated with the concentration of flavan-3-ol and oligomeric proanthocyanidins. These results suggest that moderate RDI protocols such as 70% ETc and 25/100% ETc impact positively the fruity aroma component (black and red fruit), whereas extended maceration lowered fruity aromas, possibly due to the masking effect of the oxidized character perceived in these wines.EEA MendozaFil: Casassa, Luis Federico. Washington State University. Irrigated Agriculture Research and Extension Center. School of Food Science; Estados Unidos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza. Centro de Estudios de Enología; Argentina.Fil: Larsen, Richard C. Washington State University. Viticulture and Enology Program; Estados UnidosFil: Beaver, Christopher W. Washington State University. Irrigated Agriculture Research and Extension Center. School of Food Science; Estados UnidosFil: Mireles, María S. Washington State University. Irrigated Agriculture Research and Extension Center. School of Food Science; Estados UnidosFil: Keller, Markus. Washington State University. Department of Horticulture; Estados UnidosFil: Riley, William R. Ste. Michelle Wine Estates; Estados UnidosFil: Smithyman, Russell. Ste. Michelle Wine Estates; Estados UnidosFil: Harbertson, James F. Washington State University. Irrigated Agriculture Research and Extension Center. School of Food Science; Estados Unido

Biased Signaling of CCL21 and CCL19 Does Not Rely on N-Terminal Differences, but Markedly on the Chemokine Core Domains and Extracellular Loop 2 of CCR7

Chemokine receptors play important roles in the immune system and are linked to several human diseases. Targeting chemokine receptors have so far shown very little success owing to, to some extent, the promiscuity of the immune system and the high degree of biased signaling within it. CCR7 and its two endogenous ligands display biased signaling and here we investigate the differences between the two ligands, CCL21 and CCL19, with respect to their biased activation of CCR7. We use bystander bioluminescence resonance energy transfer (BRET) based signaling assays and Transwell migration assays to determine (A) how swapping of domains between the two ligands affect their signaling patterns and (B) how receptor mutagenesis impacts signaling. Using chimeric ligands we find that the chemokine core domains are central for determining signaling outcome as the lack of β-arrestin-2 recruitment displayed by CCL21 is linked to its core domain and not N-terminus. Through a mutagenesis screen, we identify the extracellular domains of CCR7 to be important for both ligands and show that the two chemokines interact differentially with extracellular loop 2 (ECL-2). By using in silico modeling, we propose a link between ECL-2 interaction and CCR7 signal transduction. Our mutagenesis study also suggests a lysine in the top of TM3, K1303.26, to be important for G protein signaling, but not β-arrestin-2 recruitment. Taken together, the bias in CCR7 between CCL19 and CCL21 relies on the chemokine core domains, where interactions with ECL-2 seem particularly important. Moreover, TM3 selectively regulates G protein signaling as found for other chemokine receptors.publishe

Sediment redistribution beneath the terminus of an advancing glacier, Taku Glacier (T’aakú Kwáan Sít’i), Alaska.

The recently-advancing Taku Glacier is excavating subglacial sediments at high rates over multidecadal timescales. However, sediment redistribution over shorter timescales remains unquantified. We use a variety of methods to study subglacial and proglacial sediment redistribution on decadal, seasonal, and daily timescales to gain insight into sub- and proglacial landscape formation. Both excavation and deposition were observed from 2003 to 2015 (2.8 ± 0.9ma−1 to +2.9 ± 0.9ma−1). The observed patterns imply that a subglacial conduit has occupied the same site over the past decade. Outwash fans on the subaerial end moraine experience fluvial sediment reworking almost year-round, with net sediment gain in winter and net sediment loss in summer, and an overall mass gain between 2005 and 2015.We estimate that tens of meters of sediment still underlie the glacier terminus, sediments which can be remobilized during future activity. However, imminent retreat from the proglacial moraine will limit its sediment supply, leaving the moraine vulnerable to erosion by bordering rivers. Retreat into an over-deepened basin will leave the glacier vulnerable to increased frontal ablation and accelerating retreat.Ye

Special topic: The association between pulse ingredients and canine dilated cardiomyopathy: addressing the knowledge gaps before establishing causation.

In July 2018, the Food and Drug Administration warned about a possible relationship between dilated cardiomyopathy (DCM) in dogs and the consumption of dog food formulated with potatoes and pulse ingredients. This issue may impede utilization of pulse ingredients in dog food or consideration of alternative proteins. Pulse ingredients have been used in the pet food industry for over 2 decades and represent a valuable source of protein to compliment animal-based ingredients. Moreover, individual ingredients used in commercial foods do not represent the final nutrient concentration of the complete diet. Thus, nutritionists formulating dog food must balance complementary ingredients to fulfill the animal's nutrient needs in the final diet. There are multiple factors that should be considered, including differences in nutrient digestibility and overall bioavailability, the fermentability and quantity of fiber, and interactions among food constituents that can increase the risk of DCM development. Taurine is a dispensable amino acid that has been linked to DCM in dogs. As such, adequate supply of taurine and/or precursors for taurine synthesis plays an important role in preventing DCM. However, requirements of amino acids in dogs are not well investigated and are presented in total dietary content basis which does not account for bioavailability or digestibility. Similarly, any nutrient (e.g., soluble and fermentable fiber) or physiological condition (e.g., size of the dog, sex, and age) that increases the requirement for taurine will also augment the possibility for DCM development. Dog food formulators should have a deep knowledge of processing methodologies and nutrient interactions beyond meeting the Association of American Feed Control Officials nutrient profiles and should not carelessly follow unsubstantiated market trends. Vegetable ingredients, including pulses, are nutritious and can be used in combination with complementary ingredients to meet the nutritional needs of the dog