Joining as a skill in structural family therapy

This study is for family therapists.It reviews theory and techniques on the topic of joining in structural family therapy.It offers a way with clients, which can be internally reflected on, investigated, argued about,integrated and finally accepted,ignored or modified. Action research was selected as the research design. The topic which was investigated was a family system seen during practicalsessions at Unisa.Social WorkM.A. (Social Science (Mental Health)

Transcriptome analysis by GeneTrail revealed regulation of functional categories in response to alterations of iron homeostasis in Arabidopsis thaliana

<p>Abstract</p> <p>Background</p> <p>High-throughput technologies have opened new avenues to study biological processes and pathways. The interpretation of the immense amount of data sets generated nowadays needs to be facilitated in order to enable biologists to identify complex gene networks and functional pathways. To cope with this task multiple computer-based programs have been developed. GeneTrail is a freely available online tool that screens comparative transcriptomic data for differentially regulated functional categories and biological pathways extracted from common data bases like KEGG, Gene Ontology (GO), TRANSPATH and TRANSFAC. Additionally, GeneTrail offers a feature that allows screening of individually defined biological categories that are relevant for the respective research topic.</p> <p>Results</p> <p>We have set up GeneTrail for the use of <it>Arabidopsis thaliana</it>. To test the functionality of this tool for plant analysis, we generated transcriptome data of root and leaf responses to Fe deficiency and the Arabidopsis metal homeostasis mutant <it>nas4x-1</it>. We performed Gene Set Enrichment Analysis (GSEA) with eight meaningful pairwise comparisons of transcriptome data sets. We were able to uncover several functional pathways including metal homeostasis that were affected in our experimental situations. Representation of the differentially regulated functional categories in Venn diagrams uncovered regulatory networks at the level of whole functional pathways. Over-Representation Analysis (ORA) of differentially regulated genes identified in pairwise comparisons revealed specific functional plant physiological categories as major targets upon Fe deficiency and in <it>nas4x-1</it>.</p> <p>Conclusion</p> <p>Here, we obtained supporting evidence, that the <it>nas4x-1 </it>mutant was defective in metal homeostasis. It was confirmed that <it>nas4x-1 </it>showed Fe deficiency in roots and signs of Fe deficiency and Fe sufficiency in leaves. Besides metal homeostasis, biotic stress, root carbohydrate, leaf photosystem and specific cell biological categories were discovered as main targets for regulated changes in response to - Fe and <it>nas4x-1</it>. Among 258 differentially expressed genes in response to - Fe and <it>nas4x-1 </it>five functional categories were enriched covering metal homeostasis, redox regulation, cell division and histone acetylation. We proved that GeneTrail offers a flexible and user-adapted way to identify functional categories in large-scale plant transcriptome data sets. The distinguished feature that allowed analysis of individually assembled functional categories facilitated the study of the <it>Arabidopsis thaliana </it>transcriptome.</p

Avaliando a economia circular nas indústrias brasileiras pelo processo de hierarquia analítica

O desenvolvimento sustentável tem sido buscado por organizações em&nbsp;todo o mundo desde que as questões ambientais e sociais entraram nas&nbsp;agendas institucionais. Nos diversos setores da economia, os fatores que&nbsp;influenciam as decisões sustentáveis são multidisciplinares, sistêmicos e&nbsp;abordam o conceito de economia circular (EC). O objetivo deste estudo&nbsp;foi desenvolver um método para medir o nível de comprometimento&nbsp;de empresas e setores com a EC. O método permite investigar fatores&nbsp;institucionais relacionados ao desenvolvimento sustentável e avaliar a&nbsp;profundidade das práticas de EC. Foi gerado um índice de circularidade&nbsp;que pode auxiliar os tomadores de decisão no desenvolvimento de&nbsp;estratégias, planos de investimento e políticas específicas capazes de&nbsp;orientar as organizações para o alcance da EC. A metodologia proposta&nbsp;foi então aplicada a 75 empresas brasileiras reconhecidas por suas&nbsp;iniciativas de sustentabilidade, analisando-se as práticas relacionadas&nbsp;às ações de EC, bem como sua profundidade. Os resultados, utilizando&nbsp;o Processo Analítico Hierárquico (PAH), mostram que os setores&nbsp;analisados não apresentam diferença significativa entre si, e que a&nbsp;maioria das empresas analisadas (80%) não pratica ação circular alguma,&nbsp;apesar de afirmar o contrário. Sendo assim, a EC ainda é incipiente no&nbsp;país. A aplicação do método proposto em uma grande amostra mostrou&nbsp;seu potencial de uso global, podendo também ser empregada para&nbsp;orientar ações de empresas isoladas ou de setores inteiros na direção&nbsp;do desenvolvimento sustentável, por meio da EC.Sustainable development has been pursued by organizations&nbsp;around the world ever since environmental and social issues&nbsp;were introduced into institutional agendas. In the various sectors&nbsp;of the economy, the factors that influence sustainable decisions&nbsp;are multidisciplinary and systemic, and address the concept of&nbsp;Circular Economy (CE). This study aimed to develop a method to&nbsp;measure the level of commitment of companies and sectors to CE.&nbsp;The method allows investigating institutional factors associated with&nbsp;sustainable development and assessing the depth of CE practices.&nbsp;A circularity index is originated that can assist decision makers in the&nbsp;development of specific strategies, investment plans, and policies to&nbsp;guide organizations towards the achievement of a CE. The proposed&nbsp;method was then applied to 75 Brazilian companies recognized for&nbsp;their sustainability initiatives, analyzing practices associated with&nbsp;CE actions, as well as their depth. The results, using the Analytic&nbsp;Hierarchical Process (AHP), indicate that the sectors analyzed do not&nbsp;have a significant difference among them and that the majority of&nbsp;the companies analyzed (80%) do not practice any circular action&nbsp;despite claiming the opposite. Therefore, CE is still incipient in Brazil.&nbsp;The application of the proposed method to a large sample showed&nbsp;its potential for global use, and that it can also be employed to guide&nbsp;actions of single companies or entire sectors towards sustainable&nbsp;development using a CE path

Understanding and application of daptomycin-susceptible dose-dependent category for Enterococcus: A mixed-methods study

Background: In 2018, the Clinical Microbiology Laboratory at our institution adopted updated daptomycin Methods: This mixed-methods study combined a clinician survey with a retrospective pre-post prescribing analysis. An 8-question survey was distributed to infectious diseases (ID) and internal medicine (IM) clinicians. A retrospective chart review of hospitalized adults with infections due to Results: Survey response rates were 40 of 98 (41%) for IM and 22 of 34 (65%) for ID clinicians. ID clinicians scored significantly higher than IM clinicians in knowledge of SDD. Chart review of 474 patients (225 pre- vs 249 post-SDD) showed that daptomycin dosage following susceptibility testing was significantly higher post-SDD compared with pre-SDD (8.5 mg/kg vs 6.4 mg/kg; Conclusions: The survey revealed that ID clinicians placed more importance on and had more confidence in the SDD category over IM clinicians. SDD reporting was associated with a change in definitive daptomycin dosing. ID specialist involvement is recommended in the care of infections due to enterococci for which daptomycin is reported as SDD given their expertise

Effect of d-cycloserine on fear extinction training in adults with social anxiety disorder

© 2019 Hofmann et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Preclinical and clinical data have shown that D-cycloserine (DCS), a partial agonist at the N-methyl-d-aspartate receptor complex, augments the retention of fear extinction in animals and the therapeutic learning from exposure therapy in humans. However, studies with nonclinical human samples in de novo fear conditioning paradigms have demonstrated minimal to no benefit of DCS. The aim of this study was to evaluate the effects of DCS on the retention of extinction learning following de novo fear conditioning in a clinical sample. Eighty-one patients with social anxiety disorder were recruited and underwent a previously validated de novo fear conditioning and extinction paradigm over the course of three days. Of those, only 43 (53%) provided analyzable data. During conditioning on Day 1, participants viewed images of differently colored lamps, two of which were followed by with electric shock (CS+) and a third which was not (CS-). On Day 2, participants were randomly assigned to receive either 50 mg DCS or placebo, administered in a double-blind manner 1 hour prior to extinction training with a single CS+ in a distinct context. Day 3 consisted of tests of extinction recall and renewal. The primary outcome was skin conductance response to conditioned stimuli, and shock expectancy ratings were examined as a secondary outcome. Results showed greater skin conductance and expectancy ratings in response to the CS+ compared to CS- at the end of conditioning. As expected, this difference was no longer present at the end of extinction training, but returned at early recall and renewal phases on Day 3, showing evidence of return of fear. In contrast to hypotheses, DCS had no moderating influence on skin conductance response or expectancy of shock during recall or renewal phases. We did not find evidence of an effect of DCS on the retention of extinction learning in humans in this fear conditioning and extinction paradigm

Attenuation of pattern recognition receptor signaling is mediated by a MAP kinase kinase kinase

Pattern recognition receptors (PRRs) play a key role in plant and animal innate immunity. PRR binding of their cognate ligand triggers a signaling network and activates an immune response. Activation of PRR signaling must be controlled prior to ligand binding to prevent spurious signaling and immune activation. Flagellin perception in Arabidopsis through FLAGELLIN‐SENSITIVE 2 (FLS2) induces the activation of mitogen‐activated protein kinases (MAPKs) and immunity. However, the precise molecular mechanism that connects activated FLS2 to downstream MAPK cascades remains unknown. Here, we report the identification of a differentially phosphorylated MAP kinase kinase kinase that also interacts with FLS2. Using targeted proteomics and functional analysis, we show that MKKK7 negatively regulates flagellin‐triggered signaling and basal immunity and this requires phosphorylation of MKKK7 on specific serine residues. MKKK7 attenuates MPK6 activity and defense gene expression. Moreover, MKKK7 suppresses the reactive oxygen species burst downstream of FLS2, suggesting that MKKK7‐mediated attenuation of FLS2 signaling occurs through direct modulation of the FLS2 complex

UM PANORAMA DA PESQUISA NA FORMAÇÃO CONTINUADA EM EDUCAÇÃO AMBIENTAL NO VALE DO ITAJAÍ-SC: UM OLHAR DO GEEAS-UNIVALI

O artigo apresenta a trajetória de pesquisa desenvolvida durante processos deformação continuada em Educação Ambiental do Grupo de Pesquisa Educação,Estudos Ambientais e Sociedade (GEEAS), do Programa de Mestrado emEducação da Univali, Itajaí, Santa Catarina

Calreticulin mutations affect its chaperone function and perturb the glycoproteome

Calreticulin (CALR) is an endoplasmic reticulum (ER)-retained chaperone that assists glycoproteins in obtaining their structure. CALR mutations occur in patients with myeloproliferative neoplasms (MPNs), and the ER retention of CALR mutants (CALR MUT) is reduced due to a lacking KDEL sequence. Here, we investigate the impact of CALR mutations on protein structure and protein levels in MPNs by subjecting primary patient samples and CALR-mutated cell lines to limited proteolysis-coupled mass spectrometry (LiP-MS). Especially glycoproteins are differentially expressed and undergo profound structural alterations in granulocytes and cell lines with homozygous, but not with heterozygous, CALR mutations. Furthermore, homozygous CALR mutations and loss of CALR equally perturb glycoprotein integrity, suggesting that loss-of-function attributes of mutated CALR chaperones (CALR MUT) lead to glycoprotein maturation defects. Finally, by investigating the misfolding of the CALR glycoprotein client myeloperoxidase (MPO), we provide molecular proof of protein misfolding in the presence of homozygous CALR mutations. Keywords: CP: Cancer; CP: Molecular biology; calreticulin; chaperone; glycoprotein; limited proteolysis-coupled mass spectrometry; myeloperoxidase; myeloproliferative neoplasm; protein folding; proteome