15 research outputs found
In vitro dynamics of HIV-1 BF intersubtype recombinants genomic regions involved in the regulation of gene expression
HIV-1 intersubtype recombination is a very common phenomenon that has been shown to frequently affect different viral genomic regions. Vpr and Tat are viral proteins known to interact with viral promoter (LTR) during the replication cycle. This interaction is mainly involved in the regulation of viral gene expression, so, any structural changes in the LTR and/or these regulatory proteins may have an important impact on viral replication and spread. It has been reported that these genetic structures underwent recombination in BF variants widely spread in South America. To gain more insight of the consequences of the BF intersubtype recombination phenomenon on these different but functionally related genomic regions we designed and performed and in vitro study that allowed the detection and recovery of intersubtype recombinants sequences and its subsequent analysis. Our results indicate that recombination affects differentially these regions, showing evidence of a time-space relationship between the changes observed in the viral promoter and the ones observed in the Vpr/Tat coding region. This supports the idea of intersubtype recombination as a mechanism that promotes biological adaptation and compensates fitness variations
Resveratrol against Echinococcus sp.: Discrepancies between In Vitro and In Vivo Responses
In an attempt to find new anti-echinococcal drugs, resveratrol (Rsv) effectiveness against the larval stages of Echinococcus granulosus and E. multilocularis was evaluated. The in vitro effect of Rsv on parasites was assessed via optical and electron microscopy, RT-qPCR and immunohistochemistry. In vivo efficacy was evaluated in murine models of cystic (CE) and alveolar echinococcosis (AE). The impact of infection and drug treatment on the mouse bone marrow hematopoietic stem cell (HSC) population and its differentiation into dendritic cells (BMDCs) was investigated via flow cytometry and RT-qPCR. In vitro treatment with Rsv reduced E. granulosus metacestode and protoscolex viability in a concentration-dependent manner, caused ultrastructural damage, increased autophagy gene transcription, and raised Eg-Atg8 expression while suppressing Eg-TOR. However, the intraperitoneal administration of Rsv was not only ineffective, but also promoted parasite development in mice with CE and AE. In the early infection model of AE treated with Rsv, an expansion of HSCs was observed followed by their differentiation towards BMCDs. The latter showed an anti-inflammatory phenotype and reduced LPS-stimulated activation compared to control BMDCs. We suggest that Rsv ineffectiveness could have been caused by the low intracystic concentration achieved in vivo and the drug’s hormetic effect, with opposite anti-parasitic and immunomodulatory responses in different doses.Fil: Loos, Julia Alexandra. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; ArgentinaFil: Franco, Micaela. Gobierno de la Provincia de Buenos Aires. Hospital Interzonal General de Agudos Dr. Oscar Alende de Mar del Plata.; ArgentinaFil: Chop, Maia. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de QuÃmica; Argentina. Consejo Nacional de Investigaciones CientÃficas y Técnicas. Centro CientÃfico Tecnológico Conicet - Mar del Plata; ArgentinaFil: RodrÃguez RodrÃgues, Christian Fernando Ariel. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de QuÃmica; Argentina. Consejo Nacional de Investigaciones CientÃficas y Técnicas. Centro CientÃfico Tecnológico Conicet - Mar del Plata; ArgentinaFil: Cumino, Andrea Carina. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentin
Supercritical CO2 extraction of bioactive compounds from radish leaves: Yield, antioxidant capacity and cytotoxicity
Supercritical Fluid Extraction by using CO2 can be employed as an alternative to conventional methods for extracting functional compounds. Overall extractions curves for extractions of bioactive compounds from radish leaves were performed using scCO2 adding ethanol as co-solvent. Yield, total phenolic compounds, total flavonoids and antioxidant capacity were determined for each extract. The best results were obtained at 35 °C/400 bar and 40 °C/400 bar, with values of total phenolics 1375 mg GAE/100 g d.m. and 1455 mg GAE/100 g d.m., respectively. Antioxidant capacity by DPPH values were 359 mg TE/100 g d.m. (35 °C/400 bar) and 403 mg TE/100 g d.m. (40 °C/400 bar). Maturation of dendritic cells was not promoted being these extracts promising and interesting sources of antioxidative and anti-inflammatory agents for future uses in the pharmaceutical and alimentary industries.Fil: Goyeneche, Rosario. Universidad Nacional de Mar del Plata. Facultad de IngenierÃa. Departamento de IngenierÃa QuÃmica. Grupo de Investigación en IngenierÃa en Alimentos; ArgentinaFil: Fanovich, Maria Alejandra. Consejo Nacional de Investigaciones CientÃficas y Técnicas. Centro CientÃfico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y TecnologÃa de Materiales. Universidad Nacional de Mar del Plata. Facultad de IngenierÃa. Instituto de Investigaciones en Ciencia y TecnologÃa de Materiales; ArgentinaFil: RodrÃguez RodrÃgues, Christian Fernando Ariel. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Nicolao, MarÃa Celeste. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Di Scala, Karina Cecilia. Universidad Nacional de Mar del Plata. Facultad de IngenierÃa. Departamento de IngenierÃa QuÃmica. Grupo de Investigación en IngenierÃa en Alimentos; Argentin
Hydatid fluid from Echinococcus granulosus induces phagophore and autophagosome formation in dendritic cells through an upregulation of Beclin-1
Background: The cestode Echinococcus granulosus (Eg) is the etiological agent of cystic echinococcosis. This parasite develops cysts filled with hydatid fluid (HF) in the viscera of the intermediate host. Autophagy is a cellular catabolic process that plays a key role in the presentation of endogenous and exogenous proteins, promoting the activation of T cells. The aim of this work is to analyze if HF, constituted by a wide range of parasite proteins, could trigger autophagy in dendritic cells. Methods: HF was punctured from the hydatid cysts collected of infected cattle slaughtered. Murine BMDCs were cultured in RPMI 1640 medium, supplemented with FLT3-L. First, lysosome activity was evaluated using Acridine Orange, a fluorophore that can be trapped in acidic vesicular organelles. Then, autophagy induction was evaluated by FACS, qPCR, Confocal and Transmission Electron Microscopy. Rapamycin (20 nM) and chloroquine (100μM) were used to modulate autophagic flux. LC3-attachment to the autophagic membrane, were analyzed by stained DCs with anti LC3-β antibody (clone H50). Results: HF significantly increased acridine orange cytoplasmic accumulation compared to control cells (***p <0.001) and enhanced the effect of rapamycin (****p<0.0001). The ultrastructural analysis of TEM showed that in the presence of HF, DCs stimulate the formation of phagophores, double lipid membrane autophagosome, MVBs and autolysosomes. Also, HF-stimulated BMDCs significantly enhanced the mean fluorescence intensity of LC3-positive structures in comparison with unstimulated cells (*p<0.05, **p<0.01, ***p<0.001 HF-stimulated cells vs controls). Finally, we have observed that HF induces a significant increase in the transcriptional expression of LC3 and Beclin-1 (n=3, **p <0.01 vs control) and enhances the expression induced by rapamycin. Conclusions: These results suggest that HF of Echinococcus granulosus regulates gene expression to increase autophagy- related structures in DCs.Fil: Chop, Maia. Universidad Nacional de Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente - Consejo Nacional de Investigaciones CientÃficas y Técnicas. Centro CientÃfico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente; ArgentinaFil: Nicolao, MarÃa Celeste. Universidad Nacional de Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente - Consejo Nacional de Investigaciones CientÃficas y Técnicas. Centro CientÃfico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente; ArgentinaFil: Loos, Julia Alexandra. Universidad Nacional de Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente - Consejo Nacional de Investigaciones CientÃficas y Técnicas. Centro CientÃfico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente; ArgentinaFil: Ledo, Camila. Universidad Nacional de Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente - Consejo Nacional de Investigaciones CientÃficas y Técnicas. Centro CientÃfico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente; ArgentinaFil: Cumino, Andrea Carina. Universidad Nacional de Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente - Consejo Nacional de Investigaciones CientÃficas y Técnicas. Centro CientÃfico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente; ArgentinaFil: RodrÃguez RodrÃgues, Christian Fernando Ariel. Universidad Nacional de Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente - Consejo Nacional de Investigaciones CientÃficas y Técnicas. Centro CientÃfico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente; ArgentinaLXVI Reunión Anual de la Sociedad Argentina de Investigación ClÃnica; LXIX Reunión Anual de la Sociedad Argentina de InmunologÃa; LIII Reunión Anual de la Asociación de FarmacologÃa Experimental y XI Reunión Anual de la Asociación Argentina de NanomedicinasArgentinaSociedad Argentina de Investigación ClÃnicaSociedad Argentina de InmunologÃaAsociación de FarmacologÃa ExperimentalAsociación Argentina de Nanomedicina
Characterization of protein cargo of Echinococcus granulosus extracellular vesicles in drug response and its influence on immune response
Background: The Echinococcus granulosus sensu lato species complex causes cystic echinococcosis, a zoonotic disease of medical importance. Parasite-derived small extracellular vesicles (sEVs) are involved in the interaction with hosts intervening in signal transduction related to parasite proliferation and disease pathogenesis. Although the characteristics of sEVs from E. granulosus protoscoleces and their interaction with host dendritic cells (DCs) have been described, the effect of sEVs recovered during parasite pharmacological treatment on the immune response remains unexplored. Methods: Here, we isolated and characterized sEVs from control and drug-treated protoscoleces by ultracentrifugation, transmission electron microscopy, dynamic light scattering, and proteomic analysis. In addition, we evaluated the cytokine response profile induced in murine bone marrow-derived dendritic cells (BMDCs) by qPCR. Results: The isolated sEVs, with conventional size between 50 and 200 nm, regardless of drug treatment, showed more than 500 cargo proteins and, importantly, 20 known antigens and 70 potential antigenic proteins, and several integral-transmembrane and soluble proteins mainly associated with signal transduction, immunomodulation, scaffolding factors, extracellular matrix-anchoring, and lipid transport. The identity and abundance of proteins in the sEV-cargo from metformin- and albendazole sulfoxide (ABZSO)-treated parasites were determined by proteomic analysis, detecting 107 and eight exclusive proteins, respectively, which include proteins related to the mechanisms of drug action. We also determined that the interaction of murine BMDCs with sEVs derived from control parasites and those treated with ABZSO and metformin increased the expression of pro-inflammatory cytokines such as IL-12 compared to control cells. Additionally, protoscolex-derived vesicles from metformin treatments induced the production of IL-6, TNF-α, and IL-10. However, the expression of IL-23 and TGF-β was downregulated. Conclusions: We demonstrated that sEV-cargo derived from drug-treated E. granulosus protoscoleces have immunomodulatory functions, as they enhance DC activation towards a type 1 pro-inflammatory profile against the parasite, and therefore support the proposal of a new approach for the prevention and treatment of secondary echinococcosis.Fil: Nicolao, MarÃa Celeste. Universidad Nacional de Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente - Consejo Nacional de Investigaciones CientÃficas y Técnicas. Centro CientÃfico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente; ArgentinaFil: RodrÃguez RodrÃgues, Christian Fernando Ariel. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de QuÃmica; Argentina. Consejo Nacional de Investigaciones CientÃficas y Técnicas. Centro CientÃfico Tecnológico Conicet - Mar del Plata; ArgentinaFil: Coccimiglio, Magalà Belén. Universidad Nacional de Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente - Consejo Nacional de Investigaciones CientÃficas y Técnicas. Centro CientÃfico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente; ArgentinaFil: Ledo, Camila. Universidad Nacional de Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente - Consejo Nacional de Investigaciones CientÃficas y Técnicas. Centro CientÃfico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente; ArgentinaFil: Docena, Guillermo H.. Consejo Nacional de Investigaciones CientÃficas y Técnicas. Centro CientÃfico Tecnológico Conicet - La Plata. Instituto de Estudios Inmunológicos y Fisiopatológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Estudios Inmunológicos y Fisiopatológicos; ArgentinaFil: Cumino, Andrea Carina. Universidad Nacional de Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente - Consejo Nacional de Investigaciones CientÃficas y Técnicas. Centro CientÃfico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente; Argentin
Spermatozoa capture HIV-1 through heparan sulfate and efficiently transmit the virus to dendritic cells
Semen is the main vector for HIV-1 dissemination worldwide. It contains three major sources of infectious virus: free virions, infected leukocytes, and spermatozoa-associated virions. We focused on the interaction of HIV-1 with human spermatozoa and dendritic cells (DCs). We report that heparan sulfate is expressed in spermatozoa and plays an important role in the capture of HIV-1. Spermatozoa-attached virus is efficiently transmitted to DCs, macrophages, and T cells. Interaction of spermatozoa with DCs not only leads to the transmission of HIV-1 and the internalization of the spermatozoa but also results in the phenotypic maturation of DCs and the production of IL-10 but not IL-12p70. At low values of extracellular pH (∼6.5 pH units), similar to those found in the vaginal mucosa after sexual intercourse, the binding of HIV-1 to the spermatozoa and the consequent transmission of HIV-1 to DCs were strongly enhanced. Our observations support the notion that far from being a passive carrier, spermatozoa acting in concert with DCs might affect the early course of sexual transmission of HIV-1 infection
Mucins and polysaccharides from Echinococcus granulosus laminar layer induce a mild maturation phenotype in dendritic cells and promote gene expression of pro-inflammatory cytokines
Background: The laminar layer is an acellular structure rich in glycans that surrounds the metacestode, creating a mechanical and immunological protective barrier, crucial in the Echinococcus?host interface. The aim of this work is to analyze if purified laminar layer (pLL) could trigger maturation and cytokine production in dendritic cells by activating mTOR pathway, a central regulator of cell metabolism and environmental signals. Methods: BMDCs were cultured in complete RPMI supplemented with 100 ng/ml Flt3-L.The germinal layer was removed from pLL by washing with 2M NaCl. Flow cytometry: FITC or PE-conjugated mAbs directed to CD11c, CD40, CD80, CD86, MHC I and MHC II were used. Immunobloting and confocal microscopy: mouse anti-puromycin and rabbit anti-Phospho-mTOR antibodies were used. Gene expression analysis of IL-10, IL-12p35, IL-6, TNF-, IL-23p19, TGF- was performed on a Real Time PCR System Results: The pLL of Echinococcus induce a non-statistically significant, but a trend in the up-regulation of CD86 and MHC II and that expression change was diminished by the use of rapamycin (n=5). Conversely, no changes in the expression of CD40, CD80 or MHC I were registered. Further studies were done to analyze whether pLL was also able to stimulate the production of cytokines by BMDCs. When BMDCs were stimulated with pLL induce the expression of IL-6 and TNF-an=3 *p<0.05, **p<0.01), but not differences compared to control were observed in IL-12, IL-23, IL-10 and TGF-. Finally, we evaluated global protein synthesis and phosphorylation of mTOR using confocal microscopy or western blot. pLL (MFI:_979) showed an increase in translation levels compared to its counterpart without stimulation (MFI:_686, *p<0.05 n=3) and also induce an increase in mTOR phosphorylation levels compared to untreated BMCDs (n=3, p<0.01) Conclusions: These data suggest that Echinococcus pLL are recognized by DCs and induce activation of mTOR pathway favoring cell activation.Fil: Plá, Natalia. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de QuÃmica; ArgentinaFil: Chop, Maia. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de QuÃmica; Argentina. Consejo Nacional de Investigaciones CientÃficas y Técnicas. Centro CientÃfico Tecnológico Conicet - Mar del Plata; ArgentinaFil: Nicolao, MarÃa Celeste. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; ArgentinaFil: Cumino, Andrea Carina. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; ArgentinaFil: RodrÃguez RodrÃgues, Christian Fernando Ariel. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; ArgentinaReunión de Sociedades de BiocienciasMar del PlataArgentinaSociedad Argentina de Investigación ClÃnicaSociedad Argentina de InmunologÃaSociedad Argentina de FisiologÃ
Metformin improves the therapeutic efficacy of low-dose albendazole against experimental alveolar echinococcosis
Alveolar echinococcosis (AE) is a severe disease caused by Echinococcus multilocularis. Its chemotherapeutic treatment is based on benzimidazoles, which are rarely curative and cause several adverse effects. Therefore, it is necessary to develop alternative and safer chemotherapeutic strategies against AE. It has previously been shown that metformin (Met) exhibits considerable in vivo activity on an early-infection model of AE when administered at 50 mg kg-1 day-1 for 8 weeks. Here, the challenge is heightened by a 2-fold increase in parasite inoculum or by starting the treatment 6 weeks post-infection. In both cases, only the combination of Met (100 mg kg-1 day-1) together with a sub-optimal dose of albendazole (ABZ) (5 mg kg-1 day-1) led to a significant reduction in parasite weight compared to the untreated group. Coincidentally, drug combination showed the highest level of damage in E. multilocularis metacestodes. Likewise, Met alone or combined with ABZ led to a decrease in parasite glucose availability, which was evidenced as a lower intracystic glucose concentration. Therefore, the results demonstrate that combination therapy with Met and ABZ offers an alternative to improve the efficacy and reduce the toxicity of the high-dose ABZ monotherapy currently employed.Fil: Loos, Julia Alexandra. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones en Sanidad Producción y Ambiente. - Comisión de Investigaciones CientÃficas de la Provincia de Buenos Aires. Instituto de Investigaciones en Sanidad Producción y Ambiente; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de BiologÃa. Laboratorio de Zoonosis Parasitarias; ArgentinaFil: Coccimiglio, Magalà Belén. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de BiologÃa. Laboratorio de Zoonosis Parasitarias; ArgentinaFil: Nicolao, MarÃa Celeste. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones en Sanidad Producción y Ambiente. - Comisión de Investigaciones CientÃficas de la Provincia de Buenos Aires. Instituto de Investigaciones en Sanidad Producción y Ambiente; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de BiologÃa. Laboratorio de Zoonosis Parasitarias; ArgentinaFil: RodrÃguez RodrÃgues, Christian Fernando Ariel. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones en Sanidad Producción y Ambiente. - Comisión de Investigaciones CientÃficas de la Provincia de Buenos Aires. Instituto de Investigaciones en Sanidad Producción y Ambiente; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de QuÃmica; ArgentinaFil: Cumino, Andrea Carina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones en Sanidad Producción y Ambiente. - Comisión de Investigaciones CientÃficas de la Provincia de Buenos Aires. Instituto de Investigaciones en Sanidad Producción y Ambiente; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de BiologÃa. Laboratorio de Zoonosis Parasitarias; Argentin
Hydatid fluid from Echinococcus granulosus induce the autophagy process in dendritic cells and promote antigen presentation and T- cell proliferation
Background: Autophagy is an important process for the presentation of endogenous and exogenous proteins on MHC I and II molecules, promoting activation of CD8+ and CD4+ T cells respectively. The aim of this work is to analyze if hydatid fluid (HF) from Echinococcus, constituted by a wide range of parasite proteins could trigger autophagy improving antigen presentation and T cell proliferation. Methods: BMDCs were cultured in complete RPMI. Hydatid fluid (HF) was punctured from the hydatid cysts collected of infected cattle slaughtered. Antigen uptake was measured with (FITC-OVA) in BMDCs using a standard method. HF-stimulated BMDCs, were evaluated in autophagy induction and MHC II expression. For it, fixed cells were immunostained with LC3-clone H50 and analyzed them by immunofluorescence confocal microscopy. CFSE-stained splenocytes were co-incubated with BMDCs using a DC: splenocyte ratio of 1:4 Cellular proliferation was assayed after 4 days of culture by flow cytometry. Results: First, we evaluated if stimulation of HF during 18 h in BMDCs, induce different rates of antigen uptake. Effectively, the presence of Echinococcus antigens induces a markedly decreased OVA-uptake compared to control (**p <0.01, n=3). Next, we studied if stimulation with Eg antigens induces changes in the basal level of autophagy. HF-stimulated BMDCs significantly enhanced the mean fluorescence intensity of MHC II and LC3 and showed a trend in the increment of number and the average size of LC3-positive structures in comparison with unstimulated cells (*p<0.05, **p<0.01, ***p<0.001 HF-stimulated cells vs controls). Finally, we observed that culture splenocytes in the presence of stimulated DC induce their proliferation % CFSE+ cells CTRL:99%, HF:55% (***p<0.001, n=3). Conclusions: These data suggest that HF of Echinococcus induces an increase in autophagy processes promoting the presentation of exogenous antigens presented in MHC II molecules to improve T cell proliferation.Fil: Chop, Maia. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de QuÃmica; Argentina. Consejo Nacional de Investigaciones CientÃficas y Técnicas. Centro CientÃfico Tecnológico Conicet - Mar del Plata; ArgentinaFil: Plá, Natalia. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de QuÃmica; ArgentinaFil: Loos, Julia Alexandra. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; ArgentinaFil: Nicolao, MarÃa Celeste. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; ArgentinaFil: Cumino, Andrea Carina. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; ArgentinaFil: RodrÃguez RodrÃgues, Christian Fernando Ariel. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; ArgentinaReunión de Sociedades de BiocienciasMar del PlataArgentinaSociedad Argentina de Investigación ClÃnicaSociedad Argentina de InmunologÃaSociedad Argentina de FisiologÃ