961 research outputs found
Absolute diffuse calibration of IRAC through mid-infrared and radio study of HII regions
We investigate the diffuse absolute calibration of the InfraRed Array Camera
on the Spitzer Space Telescope at 8.0microns using a sample of 43 HII regions
with a wide range of morphologies near GLON=312deg. For each region we
carefully measure sky-subtracted,point-source- subtracted, areally-integrated
IRAC 8.0-micron fluxes and compare these with Midcourse Space eXperiment (MSX)
8.3-micron images at two different spatial resolutions, and with radio
continuum maps. We determine an accurate median ratio of IRAC
8.0-micron/MSX\8.3-micron fluxes, of 1.55+/-0.15. From robust spectral energy
distributions of these regions we conclude that the present 8.0-micron diffuse
calibration of the SST is 36% too high compared with the MSX validated
calibration, perhaps due to scattered light inside the camera. This is an
independent confirmation of the result derived for the diffuse calibration of
IRAC by the Spitzer Science Center (SSC).
From regression analyses we find that 843-MHz radio fluxes of HII regions and
mid-infrared (MIR) fluxes are linearly related for MSX at 8.3-microns and
Spitzer at 8.0 microns, confirming the earlier MSX result by Cohen & Green. The
median ratio of MIR/843-MHz diffuse continuum fluxes is 600 times smaller in
nonthermal than thermal regions, making it a sharp discriminant. The ratios are
largely independent of morphology up to a size of ~24 arcsec. We provide
homogeneous radio and MIR morphologies for all sources. MIR morphology is not
uniquely related to radio structure. Compact regions may have MIR filaments
and/or diffuse haloes, perhaps infrared counter- parts to weakly ionized radio
haloes found around compact HII regions. We offer two IRAC colour-colour plots
as quantitative diagnostics of diffuse HII regions.Comment: 29 pages, LaTeX (aastex), incl. 31 PostScript (ps,eps) figures and 5
tables. Accepted by MNRAS (main journal). Replaced an unused file and added
this URL for people wishing to download a version with high-resolution
images: http://www.astro.wisc.edu/sirtf/martin.hii.accepted.pd
A primary fish gill cell culture model to assess pharmaceutical uptake and efflux:evidence for passive and facilitated transport
AbstractThe gill is the principle site of xenobiotic transfer to and from the aqueous environment. To replace, refine or reduce (3Rs) the large numbers of fish used in in vivo uptake studies an effective in vitro screen is required that mimics the function of the teleost gill. This study uses a rainbow trout (Oncorhynchus mykiss) primary gill cell culture system grown on permeable inserts, which tolerates apical freshwater thus mimicking the intact organ, to assess the uptake and efflux of pharmaceuticals across the gill. Bidirectional transport studies in media of seven pharmaceuticals (propranolol, metoprolol, atenolol, formoterol, terbutaline, ranitidine and imipramine) showed they were transported transcellularly across the epithelium. However, studies conducted in water showed enhanced uptake of propranolol, ranitidine and imipramine. Concentration-equilibrated conditions without a concentration gradient suggested that a proportion of the uptake of propranolol and imipramine is via a carrier-mediated process. Further study using propranolol showed that its transport is pH-dependent and at very low environmentally relevant concentrations (ngL−1), transport deviated from linearity. At higher concentrations, passive uptake dominated. Known inhibitors of drug transport proteins; cimetidine, MK571, cyclosporine A and quinidine inhibited propranolol uptake, whilst amantadine and verapamil were without effect. Together this suggests the involvement of specific members of SLC and ABC drug transporter families in pharmaceutical transport
Comparison of RNAi efficiency mediated by tetracycline-responsive H1 and U6 promoter variants in mammalian cell lines
Conditional expression of short hairpin RNAs (shRNAs) to knock down target genes is a powerful tool to study gene function. The most common inducible expression systems are based on tetracycline-regulated RNA polymerase III promoters. During the last years, several tetracycline-inducible U6 and H1 promoter variants have been reported in different experimental settings showing variable efficiencies. In this study, we compare the most common variants of these promoters in several mammalian cell lines. For all cell lines tested, we find that several inducible U6 and H1 promoters containing single tetracycline operator (tetO) sequences show high-transcriptional background in the non-induced state. Promoter variants containing two tetO sequences show tight suppression of transcription in the non-induced state, and high tet responsiveness and high gene knockdown efficiency upon induction in all cell lines tested. We report a variant of the H1 promoter containing two O2-type tetO sequences flanking the TATA box that shows little transcriptional background in the non-induced state and up to 90% target knockdown when the inducer molecule (dox–doxycycline) is added. This inducible system for RNAi-based gene silencing is a good candidate for use both in basic research on gene function and for potential therapeutic applications
Conditional expression of retrovirally delivered anti-MYCN shRNA as an in vitro model system to study neuronal differentiation in MYCN-amplified neuroblastoma
<p>Abstract</p> <p>Background</p> <p>Neuroblastoma is a childhood cancer derived from immature cells of the sympathetic nervous system. The disease is clinically heterogeneous, ranging from neuronal differentiated benign ganglioneuromas to aggressive metastatic tumours with poor prognosis. Amplification of the MYCN oncogene is a well established poor prognostic factor found in up to 40% of high risk neuroblastomas.</p> <p>Using neuroblastoma cell lines to study neuronal differentiation <it>in vitro </it>is now well established. Several protocols, including exposure to various agents and growth factors, will differentiate neuroblastoma cell lines into neuron-like cells. These cells are characterized by a neuronal morphology with long extensively branched neurites and expression of several neurospecific markers.</p> <p>Results</p> <p>In this study we use retrovirally delivered inducible short-hairpin RNA (shRNA) modules to knock down <it>MYCN </it>expression in <it>MYCN</it>-amplified (MNA) neuroblastoma cell lines. By addition of the inducer doxycycline, we show that the Kelly and SK-N-BE(2) neuroblastoma cell lines efficiently differentiate into neuron-like cells with an extensive network of neurites. These cells are further characterized by increased expression of the neuronal differentiation markers <it>NFL </it>and <it>GAP43</it>. In addition, we show that induced expression of retrovirally delivered anti-<it>MYCN </it>shRNA inhibits cell proliferation by increasing the fraction of MNA neuroblastoma cells in the G1 phase of the cell cycle and that the clonogenic growth potential of these cells was also dramatically reduced.</p> <p>Conclusion</p> <p>We have developed an efficient <it>MYCN</it>-knockdown <it>in vitro </it>model system to study neuronal differentiation in MNA neuroblastomas.</p
Spitzer IRAC observations of newly-discovered planetary nebulae from the Macquarie-AAO-Strasbourg H-alpha Planetary Nebula Project
We compare H-alpha, radio continuum, and Spitzer Space Telescope (SST) images
of 58 planetary nebulae (PNe) recently discovered by the Macquarie-AAO-Strasbo-
urg H-alpha PN Project (MASH) of the SuperCOSMOS H-alpha Survey. Using InfraRed
Array Camera (IRAC) data we define the IR colors of PNe and demonstrate good
isolation between these colors and those of many other types of astronomical
object. The only substantive contamination of PNe in the color-color plane we
illustrate is due to YSOs. However, this ambiguity is readily resolved by the
unique optical characteristics of PNe and their environs. We also examine the
relationships between optical and MIR morphologies from 3.6 to 8.0um and
explore the ratio of mid-infrared (MIR) to radio nebular fluxes, which is a
valuable discriminant between thermal and nonthermal emission. MASH emphasizes
late evolutionary stages of PNe compared with previous catalogs, enabling study
of the changes in MIR and radio flux that attend the aging process. Spatially
integrated MIR energy distributions were constructed for all MASH PNe observed
by the GLIMPSE Legacy Project, using the H-alpha morphologies to establish the
dimensions for the calculations of the Midcourse Space Experiment (MSX), IRAC,
and radio continuum (from the Molonglo Observatory Synthesis Telescope and the
Very Large Array) flux densities. The ratio of IRAC 8.0-um to MSX 8.3-um flux
densities provides a measure of the absolute diffuse calibration of IRAC at 8.0
um. We independently confirm the aperture correction factor to be applied to
IRAC at 8.0um to align it with the diffuse calibration of MSX. The result
agrees with the recommendations of the Spitzer Science Center and with results
from a parallel study of HII regions. These PNe probe the diffuse calibration
of IRAC on a spatial scale of 9-77 arcsec.Comment: 48 pages, LaTeX (aastex), incl. 18 PostScript (eps) figures and 3
tables. Accepted by Astrophysical Journa
G313.3+00.3: A New Planetary Nebula discovered by the Australia Telescope Compact Array and the Spitzer Space Telescope
We present a new planetary nebula, first identified in images from the
Australia Telescope Compact Array, although not recognized at that time. Recent
observations with the Spitzer Space Telescope during the GLIMPSE Legacy program
have rediscovered the object. The high-resolution radio and infrared images
enable the identification of the central star or its wind, the recognition of
the radio emission as thermal, and the probable presence of polycylic aromatic
hydrocarbons in and around the source. These lead to the conclusion that
G313.3+00.3 is a planetary nebula. This object is of particular interest
because it was discovered solely through radio and mid-infrared imaging,
without any optical (or near-infrared) confirmation, and acts as a proof of
concept for the discovery of many more highly extinguished planetary nebulae.
G313.3+00.3 is well-resolved by both the instruments with which it was
identified, and suffers extreme reddening due to its location in the
Scutum-Crux spiral arm.Comment: 18 pages, LaTeX (aastex), incl. 8 PostScript (eps) figures and 1
table. Accepted by ApJ (Part 1
Influence of molecular symmetry on strong-field ionization: Studies on ethylene, benzene, fluorobenzene, and chlorofluorobenzene
Using the molecular strong-field approximation we consider the effects of
molecular symmetry on the ionization of molecules by a strong, linearly
polarized laser pulse. Electron angular distributions and total ionization
yields are calculated as a function of the relative orientation between the
molecule and the laser polarization. Our studies focus on ethylene
(CH), benzene (CH), fluorobenzene (CHF), and ortho
chlorofluorobenzene (1,2 CHClF), the molecules representing four
different point groups. The results are compared with experiments, when
available, and with the molecular tunneling theory appropriately extended to
non-linear polyatomic molecules. Our investigations show that the orientational
dependence of ionization yields is primarily determined by the nodal surface
structure of the molecular orbitals.Comment: 13 pages, 10 figures. Submitted to Physical Review
4-(Benzyloxy)benzaldehyde
The title compound, C14H12O2, has an essentially planar conformation with the two aromatic rings forming a dihedral angle of 5.23 (9)° and the aldehyde group lying in the plane of its aromatic group [maximum deviation = 0.204 (3) Å]. Weak intermolecular C—H⋯O contacts are found to be shortest between the aldehyde O-atom acceptor and the H atoms of the methylene group
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