Update of a database for estimation of whole grain content of foods in Australia

Food composition data is essential for calculating consumption based on reported dietary intake. Inclusion of the whole grain content of foods in food composition databases is limited. In Australia, quantification of whole grain composition does not include all foods within the current survey database, AUSNUT (Australian Food, Supplement and Nutrient) 2011-13. This study aimed to update an existing Australian whole grain database to include all foods and food products within AUSNUT 2011-13 (n = 5741). Whole grain content (g) per 100 g was calculated using a systematic recipe-based approach, and input from industry stakeholders, product packaging, and ingredient lists. Overall 590 foods were identified as containing whole grain. Cereals and cereal products formed the majority (43%) of the database. Foods with whole grain content 100.0 g/100 g were raw or puffed whole grains, whole grain flours, and ready to eat cereals made from 100% whole grains. Considerable variation in whole grain content exists between and within food groups. The updated database may be a useful tool for assessing whole grain content of Australian food intake data. Application will allow estimation of whole grain intake in highly varied Australian population groups

Novel digital food photos resource enhances knowledge of nutrition and dietetics students

A robust knowledge of food composition, the Australian food supply and cooking methods is integral to dietetics practice. Students learn these concepts across a variety of subjects, however a new dietetics subject in 2009 and a faculty based learning and teaching grant provided a timely opportunity to develop innovative new digital resources for students to enhance their food composition knowledge. One-hundred and sixty high quality digital food photographs were developed and nutrient composition data were added, before making the resource accessible to students via their eLearning sites. The primary aim of this study was to assess the views and experiences of first and fourth year students regarding the usefulness and application of the digital food photos resource in developing their food composition knowledge. The secondary aim was to identify and explore other potential applications of the resource and recommendations for future upgrades. Forty students completed an online survey and six took part in semi-structured qualitative interviews, which were facilitated by the same two researchers, before being digitally recorded and transcribed verbatim. Preliminary findings highlighted that students found the resource to be an interactive and valuable contributor to their knowledge, I loved it. I absolutely loved it. Like I sat on it for hours going through it (S1). All of the fourth year respondents said they would use the resource again, as did 83% of the first year students. A revised edition is currently being developed with 180 additional photographs and student feedback about further nutrients, symbols used and future commercial applications

Workshop to compare the indicators for CFP and MSFD D3 management objectives through simulations (WKSIMULD3)

Rapports Scientifiques du CIEM. Volume 6, nº 4The WKSIMULD3 meeting provided a platform for experts from the EU member states to meet and progress the assessment methodology on Criteria 3 of Descriptor 3 upon request by EC (DGENV). WKSIMULD3 is the third of a series of three workshops (WKD3C3SCOPE and WKD3C3THRESHOLDS being the first two) to identify operational indicators for MSFD D3C3. The workshop was organised as a series of presentations with intermittent discussions. The group agreed on a number of stocks to run simulations to explore the relationships between indicators of population traits/dynamics and healthy population structure. The participants found that for demersal stocks in the North Sea, MSE simulations showed decreasing values of D3C3 indicators with increasing fishing mortality. However, the use of age-structure indicators for these stocks is likely to lead to frequent false positives due to the high interannual variability. Age-based simulations were implemented also for pelagic and short-lived stocks, such as mackerel, sprat, and sandeel. The distribution of the threshold values implemented for the stocks did not show a clear response to the fishing regime. As a result, the use of age-structure indicators for these stocks is likely to lead to frequent false positives/negatives. The simulations aimed to validate the population size structure and calculate indicators under different fishing scenarios. In the Mediterranean Sea, length-based simulations were conducted for two hake stocks and one sole stock. The length indicators obtained were compared to historical data to assess the responsiveness of the indicators to exploitation. These results show the behaviour of the length based and of the age-based indicators calculated on observed data. Overall, the indicator status can be well below the average value at Fmsy. Additionally, an exploratory run tested a harvest control rule driven by age-based indicator targets. The outputs can provide directions on how to choose a threshold that can ensure the stock to be in GES. Overall, the workshop findings highlighted the complexity of evaluating indicators for CFP and MSFD D3 management objectives, particularly in relation to the responsiveness of the indicators to fishing pressure and environmental variation. The group emphasized the need for robust and peer-reviewed models to ensure reliable results when evaluating indicators for fish stocks. Additionally, it underscores the challenges associated with using age- and length-based indicators for different species and the importance of considering environmental and recruitment variability in simulations. In conclusion, the series of the 3 workshops contributed valuable insights into the complexities of assessing and comparing indicators for MSFD D3 management objectives, providing important considerations for future assessments and management strategies.info:eu-repo/semantics/publishedVersio

Pseudohyperphosphorylation has differential effects on polymerization and function of tau isoforms

The microtubule-associated protein tau exists as six isoforms created through the splicing of the second, third, and tenth exons. The isoforms are classified by their number of N-terminal exons (0N, 1N or 2N) and by their number of microtubule-binding repeat regions (3R or 4R). Hyperphosphorylated isoforms accumulate in insoluble aggregates in Alzheimer’s disease and other tauopathies. These neurodegenerative diseases can be categorized based on the isoform content of the aggregates they contain. Hyperphosphorylated tau has the general characteristics of an upward electrophoretic shift, decreased microtubule binding, and an association with aggregation. Previously we have shown that a combination of seven pseudophosphorylation mutations at sites phosphorylated by GSK-3β, referred to as 7-Phos, induced several of these characteristics in full-length 2N4R tau and led to the formation of fewer but longer filaments. We sought to determine whether the same phosphorylation pattern could cause differential effects in the other tau isoforms, possibly through varied conformational effects. Using in vitro techniques, we examined the electrophoretic mobility, aggregation properties and microtubule stabilization of all isoforms and their pseudophosphorylated counterparts. We found that pseudophosphorylation affected each isoform, but in several cases certain isoforms were affected more than others. These results suggest that hyperphosphorylation of tau isoforms could play a major role in determining the isoform composition of tau aggregates in disease

Dynamic Imaging of the Effector Immune Response to Listeria Infection In Vivo

Host defense against the intracellular pathogen Listeria monocytogenes (Lm) requires innate and adaptive immunity. Here, we directly imaged immune cell dynamics at Lm foci established by dendritic cells in the subcapsular red pulp (scDC) using intravital microscopy. Blood borne Lm rapidly associated with scDC. Myelomonocytic cells (MMC) swarmed around non-motile scDC forming foci from which blood flow was excluded. The depletion of scDC after foci were established resulted in a 10-fold reduction in viable Lm, while graded depletion of MMC resulted in 30–1000 fold increase in viable Lm in foci with enhanced blood flow. Effector CD8+ [CD8 superscript +] T cells at sites of infection displayed a two-tiered reduction in motility with antigen independent and antigen dependent components, including stable interactions with infected and non-infected scDC. Thus, swarming MMC contribute to control of Lm prior to development of T cell immunity by direct killing and sequestration from blood flow, while scDC appear to promote Lm survival while preferentially interacting with CD8+ [CD8 superscript +] T cells in effector sites.National Institutes of Health (U.S.) (Grant P01AI-071195

Analyzing multitarget activity landscapes using protein-ligand interaction fingerprints: interaction cliffs.

This is the original submitted version, before peer review. The final peer-reviewed version is available from ACS at http://pubs.acs.org/doi/abs/10.1021/ci500721x.Activity landscape modeling is mostly a descriptive technique that allows rationalizing continuous and discontinuous SARs. Nevertheless, the interpretation of some landscape features, especially of activity cliffs, is not straightforward. As the nature of activity cliffs depends on the ligand and the target, information regarding both should be included in the analysis. A specific way to include this information is using protein-ligand interaction fingerprints (IFPs). In this paper we report the activity landscape modeling of 507 ligand-kinase complexes (from the KLIFS database) including IFP, which facilitates the analysis and interpretation of activity cliffs. Here we introduce the structure-activity-interaction similarity (SAIS) maps that incorporate information on ligand-target contact similarity. We also introduce the concept of interaction cliffs defined as ligand-target complexes with high structural and interaction similarity but have a large potency difference of the ligands. Moreover, the information retrieved regarding the specific interaction allowed the identification of activity cliff hot spots, which help to rationalize activity cliffs from the target point of view. In general, the information provided by IFPs provides a structure-based understanding of some activity landscape features. This paper shows examples of analyses that can be carried out when IFPs are added to the activity landscape model.M-L is very grateful to CONACyT (No. 217442/312933) and the Cambridge Overseas Trust for funding. AB thanks Unilever for funding and the European Research Council for a Starting Grant (ERC-2013- StG-336159 MIXTURE). J.L.M-F. is grateful to the School of Chemistry, Department of Pharmacy of the National Autonomous University of Mexico (UNAM) for support. This work was supported by a scholarship from the Secretariat of Public Education and the Mexican government

New Frontiers-class Uranus Orbiter: Exploring the feasibility of achieving multidisciplinary science with a mid-scale mission

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Diagnosis and management of glutaric aciduria type I – revised recommendations

Glutaric aciduria type I (synonym, glutaric acidemia type I) is a rare organic aciduria. Untreated patients characteristically develop dystonia during infancy resulting in a high morbidity and mortality. The neuropathological correlate is striatal injury which results from encephalopathic crises precipitated by infectious diseases, immunizations and surgery during a finite period of brain development, or develops insidiously without clinically apparent crises. Glutaric aciduria type I is caused by inherited deficiency of glutaryl-CoA dehydrogenase which is involved in the catabolic pathways of L-lysine, L-hydroxylysine and L-tryptophan. This defect gives rise to elevated glutaric acid, 3-hydroxyglutaric acid, glutaconic acid, and glutarylcarnitine which can be detected by gas chromatography/mass spectrometry (organic acids) or tandem mass spectrometry (acylcarnitines). Glutaric aciduria type I is included in the panel of diseases that are identified by expanded newborn screening in some countries. It has been shown that in the majority of neonatally diagnosed patients striatal injury can be prevented by combined metabolic treatment. Metabolic treatment that includes a low lysine diet, carnitine supplementation and intensified emergency treatment during acute episodes of intercurrent illness should be introduced and monitored by an experienced interdisciplinary team. However, initiation of treatment after the onset of symptoms is generally not effective in preventing permanent damage. Secondary dystonia is often difficult to treat, and the efficacy of available drugs cannot be predicted precisely in individual patients. The major aim of this revision is to re-evaluate the previous diagnostic and therapeutic recommendations for patients with this disease and incorporate new research findings into the guideline

European and multi-ancestry genome-wide association meta-analysis of atopic dermatitis highlights importance of systemic immune regulation

Atopic dermatitis (AD) is a common inflammatory skin condition and prior genome-wide association studies (GWAS) have identified 71 associated loci. In the current study we conducted the largest AD GWAS to date (discovery N = 1,086,394, replication N = 3,604,027), combining previously reported cohorts with additional available data. We identified 81 loci (29 novel) in the European-only analysis (which all replicated in a separate European analysis) and 10 additional loci in the multi-ancestry analysis (3 novel). Eight variants from the multi-ancestry analysis replicated in at least one of the populations tested (European, Latino or African), while two may be specific to individuals of Japanese ancestry. AD loci showed enrichment for DNAse I hypersensitivity and eQTL associations in blood. At each locus we prioritised candidate genes by integrating multi-omic data. The implicated genes are predominantly in immune pathways of relevance to atopic inflammation and some offer drug repurposing opportunities

European and multi-ancestry genome-wide association meta-analysis of atopic dermatitis highlights importance of systemic immune regulation

Atopic dermatitis (AD) is a common inflammatory skin condition and prior genome-wide association studies (GWAS) have identified 71 associated loci. In the current study we conducted the largest AD GWAS to date (discovery N = 1,086,394, replication N = 3,604,027), combining previously reported cohorts with additional available data. We identified 81 loci (29 novel) in the European-only analysis (which all replicated in a separate European analysis) and 10 additional loci in the multi-ancestry analysis (3 novel). Eight variants from the multi-ancestry analysis replicated in at least one of the populations tested (European, Latino or African), while two may be specific to individuals of Japanese ancestry. AD loci showed enrichment for DNAse I hypersensitivity and eQTL associations in blood. At each locus we prioritised candidate genes by integrating multi-omic data. The implicated genes are predominantly in immune pathways of relevance to atopic inflammation and some offer drug repurposing opportunities.</p