Risk factors associated with Rift Valley fever epidemics in South Africa in 2008-11.

Rift Valley fever (RVF) is a zoonotic and vector-borne disease, mainly present in Africa, which represents a threat to human health, animal health and production. South Africa has experienced three major RVF epidemics (1950-51, 1973-75 and 2008-11). Due to data scarcity, no previous study has quantified risk factors associated with RVF epidemics in animals in South Africa. Using the 2008-11 epidemic datasets, a retrospective longitudinal study was conducted to identify and quantify spatial and temporal environmental factors associated with RVF incidence. Cox regressions with a Besag model to account for the spatial effects were fitted to the data. Coefficients were estimated by Bayesian inference using integrated nested Laplace approximation. An increase in vegetation density was the most important risk factor until 2010. In 2010, increased temperature was the major risk factor. In 2011, after the large 2010 epidemic wave, these associations were reversed, potentially confounded by immunity in animals, probably resulting from earlier infection and vaccination. Both vegetation density and temperature should be considered together in the development of risk management strategies. However, the crucial need for improved access to data on population at risk, animal movements and vaccine use is highlighted to improve model predictions

The P-T-t architecture of a Gondwanan suture: REE, U-Pb and Ti-in-zircon thermometric constraints from the Palghat Cauvery shear system, South India

Understanding the relationship between accessory mineral growth and the evolution of silicate mineral assemblages along the entirety of a P-T-t path is a critical step in developing models for evolving tectonic systems. Here we combine U–Pb age data (for zircon and monazite), rare earth element (REE) data and compositionally specific phase diagrams (P-T pseudosections) for the rocks of the Palghat Cauvery shear system (PCSS), Southern Indian order to constrain the periodicity of heating/cooling and burial/exhumation events during the Ediacaran/Cambrian amalgamation of Gondwana. HREE data from zircon are consistent with zircon grow that 672–724 °C during the breakdown of garnet in the kyanite stability field at 535.0 ± 4.9 Ma. This represents a cooling that punctuates the P-T-t path. Subsequent monazite growth and symplectite formation occurred at 920 °C and 7.5 kbar, 10 Ma after zircon growth which reflects a period of reheating and decompression related to delamination and the collapse of the East African orogen. The REE chemistry of the monazite is consistent with the system having undergone partial melting prior to monazite growth, thereby altering the bulk rock chemistry. The periodicity of the heating and cooling cycles (10 Ma) from this study is consistent with recently proposed tectonic switching models for the formation of granulite metamorphism in accretionary/collisional tectonic settings. The elevated heat flows required to generate the UHT metamorphism are achievable in the proposed back-arc setting for the PCSS during Gondwana amalgamation.Chris Clark, Alan S. Collins, M. Santosh, Richard Taylor and Benjamin P. Wad

The implementation and impact of the secondary science National Strategy : a single-school case study to explore the changes in classroom teaching styles and the responses of students to these initiatives

The National Strategy for Science was progressively introduced from 2002 with the intention of providing a clear structure for improved delivery of the subject in secondary schools. Through a series of scripted training events, supported by printed resources, the intention was to provide science teachers with a clear framework for sequential teaching of key themes through the use of pedagogy intended to involve the students in their own learning. After several years, the nature of the National Strategy shifted to concentrate on the support of subject leaders, and the Strategy is planned to end in 2011. The current school cohorts have all experienced the teaching of science since the introduction of the Strategy, and should therefore have benefitted from the improved delivery, intended to create improved outcomes and more positive attitudes towards science. By means of a case study investigation in 2008 in a single school, the impact of the National Strategy was explored. By means of a range of qualitative methods, including questionnaires, interviews and lesson observations, it was possible to investigate the extent to which National Strategy ideas had become embedded in the daily routines of the science teachers, and the extent to which students viewed science positively. The study focused on Y7 (soon after entry to the school), Y9 (prior to the SATs examinations) and Y11 (during the run-up to GCSE). An initial study four years previously was used to provide an indication of changes during the life of the Strategy, and to indicate trends. In addition, sampling in other schools was used to determine whether the questionnaire results were atypical. The results showed that the Strategy had largely failed to become embedded in normal classroom practice, with little evidence of teachers making good use of the pedagogy or the structured delivery that was central to the Strategy message. The reasons for this failure were: • The expectation that centrally-delivered training would be effectively cascaded by one individual to other teachers in the school, • The failure to concentrate on a few simple messages or themes, repeatedly delivered and reinforced in subsequent training, • The introduction of a plethora of other initiatives, each demanding teacher time, and diluting efforts to focus attention on the National Strategy themes. As a result, the science teachers in 2008 showed less understanding of the Strategy than teachers in 2004, and their use of techniques such as the three-part lesson and enquiry-based learning were less evident. The Strategy was to be a mechanism to improve examination results and to improve student attitudes to science. The examination results are shown to be largely stagnant over this period 2003-2008, and the attitudes of students towards science are shown to become less positive during their time in secondary school. The key finding, therefore, is that the Strategy failed in its aims because it failed to listen to its own message. It failed to recognise that teachers, just as much as students, need simple messages, repeatedly delivered in innovative ways, in order to learn and fully internalise these ideas.EThOS - Electronic Theses Online ServiceGBUnited Kingdo

High sensitivity of future global warming to land carbon cycle processes

Unknowns in future global warming are usually assumed to arise from uncertainties either in the amount of anthropogenic greenhouse gas emissions or in the sensitivity of the climate to changes in greenhouse gas concentrations. Characterizing the additional uncertainty in relating CO2 emissions to atmospheric concentrations has relied on either a small number of complex models with diversity in process representations, or simple models. To date, these models indicate that the relevant carbon cycle uncertainties are smaller than the uncertainties in physical climate feedbacks and emissions. Here, for a single emissions scenario, we use a full coupled climate–carbon cycle model and a systematic method to explore uncertainties in the land carbon cycle feedback. We find a plausible range of climate–carbon cycle feedbacks significantly larger than previously estimated. Indeed the range of CO2 concentrations arising from our single emissions scenario is greater than that previously estimated across the full range of IPCC SRES emissions scenarios with carbon cycle uncertainties ignored. The sensitivity of photosynthetic metabolism to temperature emerges as the most important uncertainty. This highlights an aspect of current land carbon modelling where there are open questions about the potential role of plant acclimation to increasing temperatures. There is an urgent need for better understanding of plant photosynthetic responses to high temperature, as these responses are shown here to be key contributors to the magnitude of future change

An infrared approach to Reggeization

We present a new approach to Reggeization of gauge amplitudes based on the universal properties of their infrared singularities. Using the "dipole formula", a compact ansatz for all infrared singularities of massless amplitudes, we study Reggeization of singular contributions to high-energy amplitudes for arbitrary color representations, and any logarithmic accuracy. We derive leading-logarithmic Reggeization for general cross-channel color exchanges, and we show that Reggeization breaks down for the imaginary part of the amplitude at next-to-leading logarithms and for the real part at next-to-next-to-leading logarithms. Our formalism applies to multiparticle amplitudes in multi-Regge kinematics, and constrains possible corrections to the dipole formula starting at three loops.Comment: 4 page

The 2dF Galaxy Redshift Survey: a targeted study of catalogued clusters of galaxies

We have carried out a study of known clusters within the 2dF Galaxy Redshift Survey (2dFGRS) observed areas and have identified 431 Abell, 173APM and 343 EDCC clusters. Precise redshifts, velocity dispersions and new centroids have been measured for the majority of these objects, and this information is used to study the completeness of these catalogues, the level of contamination from foreground and background structures along the cluster's line of sight, the space density of the clusters as a function of redshift, and their velocity dispersion distributions. We find that the Abell and EDCC catalogues are contaminated at the level of about 10 per cent, whereas the APM catalogue suffers only 5 per cent contamination. If we use the original catalogue centroids, the level of contamination rises to approximately 15 per cent for the Abell and EDCC catalogues, showing that the presence of foreground and background groups may alter the richness of clusters in these catalogues. There is a deficiency of clusters at z~0.05 that may correspond to a large underdensity in the Southern hemisphere. From the cumulative distribution of velocity dispersions for these clusters, we derive a space density of σ>1000kms-1 clusters of 3.6×10-6h3Mpc-3. This result is used to constrain models for structure formation; our data favour low-density cosmologies, subject to the usual assumptions concerning the shape and normalization of the power spectrum

A pre-post test evaluation of the impact of the PELICAN MDT-TME development programme on the working lives of colorectal cancer team members

Background - The PELICAN Multidisciplinary Team Total Mesorectal Excision (MDT-TME) Development Programme aimed to improve clinical outcomes for rectal cancer by educating colorectal cancer teams in precision surgery and related aspects of multidisciplinary care. The Programme reached almost all colorectal cancer teams across England. We took the opportunity to assess the impact of participating in this novel team-based Development Programme on the working lives of colorectal cancer team members. Methods - The impact of participating in the programme on team members' self-reported job stress, job satisfaction and team performance was assessed in a pre-post course study. 333/568 (59%) team members, from the 75 multidisciplinary teams who attended the final year of the Programme, completed questionnaires pre-course, and 6-8 weeks post-course. Results - Across all team members, the main sources of job satisfaction related to working in multidisciplinary teams; whilst feeling overloaded was the main source of job stress. Surgeons and clinical nurse specialists reported higher levels of job satisfaction than team members who do not provide direct patient care, whilst MDT coordinators reported the lowest levels of job satisfaction and job stress. Both job stress and satisfaction decreased after participating in the Programme for all team members. There was a small improvement in team performance. Conclusions - Participation in the Development Programme had a mixed impact on the working lives of team members in the immediate aftermath of attending. The decrease in team members' job stress may reflect the improved knowledge and skills conferred by the Programme. The decrease in job satisfaction may be the consequence of being unable to apply these skills immediately in clinical practice because of a lack of required infrastructure and/or equipment. In addition, whilst the Programme raised awareness of the challenges of teamworking, a greater focus on tackling these issues may have improved working lives further

Soil organic matter storage in temperate lowland arable, grassland and woodland topsoil and subsoil

Soils are the largest terrestrial pool of organic carbon, with up to 50% of soil organic carbon (SOC) stored below 30 cm. Knowledge of the impact of land use on the mechanisms by which SOC is stored in subsoils is critical to developing and delivering strategies to mitigate climate change. We characterised SOC under arable, grassland and deciduous woodland land uses in lowland England to determine how land use affects the mechanisms by which topsoil and subsoil SOC is protected. Soil organic matter (SOM) physical fractionation and ammonium oxalate extractable Al, Fe, and Mn were analysed to elucidate protection mechanisms. Results revealed that the mineral-free particulate organic matter (fPOM) fraction was significantly greater in both the topsoil and subsoil under woodland than under grassland or arable. The mineral associated organic carbon (MinOC) fraction was proportionally greater in the subsoil compared to topsoil under all land uses; with arable > grassland > woodland. These findings indicate that land use affects the extent to which SOC is protected, with woodlands containing a higher proportion of carbon that has less protection from decomposition. Subsoil SOC is protected from decomposition by organo-mineral interactions with amorphous Al, Fe and Mn, and may be susceptible to future pH shifts as a result of land use change. This study highlights the need to consider the impact of land use change on SOC, given policy and public interest in woodland planting for climate change mitigation

Targeting triple-negative breast cancer cells with the histone deacetylase inhibitor panobinostat

INTRODUCTION: Of the more than one million global cases of breast cancer diagnosed each year, approximately fifteen percent are characterized as triple-negative, lacking the estrogen, progesterone, and Her2/neu receptors. Lack of effective therapies, younger age at onset, and early metastatic spread have contributed to the poor prognoses and outcomes associated with these malignancies. Here, we investigate the ability of the histone deacetylase inhibitor panobinostat (LBH589) to selectively target triple-negative breast cancer (TNBC) cell proliferation and survival in vitro and tumorigenesis in vivo. METHODS: TNBC cell lines MDA-MB-157, MDA-MB-231, MDA-MB-468, and BT-549 were treated with nanomolar (nM) quantities of panobinostat. Relevant histone acetylation was verified by flow cytometry and immunofluorescent imaging. Assays for trypan blue viability, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) proliferation, and DNA fragmentation were used to evaluate overall cellular toxicity. Changes in cell cycle progression were assessed with propidium iodide flow cytometry. Additionally, qPCR arrays were used to probe MDA-MB-231 cells for panobinostat-induced changes in cancer biomarkers and signaling pathways. Orthotopic MDA-MB-231 and BT-549 mouse xenograft models were used to assess the effects of panobinostat on tumorigenesis. Lastly, flow cytometry, ELISA, and immunohistochemical staining were applied to detect changes in cadherin-1, E-cadherin (CDH1) protein expression and the results paired with confocal microscopy in order to examine changes in cell morphology. RESULTS: Panobinostat treatment increased histone acetylation, decreased cell proliferation and survival, and blocked cell cycle progression at G2/M with a concurrent decrease in S phase in all TNBC cell lines. Treatment also resulted in apoptosis induction at 24 hours in all lines except the MDA-MB-468 cell line. MDA-MB-231 and BT-549 tumor formation was significantly inhibited by panobinostat (10 mg/kg/day) in mice. Additionally, panobinostat up-regulated CDH1 protein in vitro and in vivo and induced cell morphology changes in MDA-MB-231 cells consistent with reversal of the mesenchymal phenotype. CONCLUSIONS: This study revealed that panobinostat is overtly toxic to TNBC cells in vitro and decreases tumorigenesis in vivo. Additionally, treatment up-regulated anti-proliferative, tumor suppressor, and epithelial marker genes in MDA-MB-231 cells and initiated a partial reversal of the epithelial-to-mesenchymal transition. Our results demonstrate a potential therapeutic role of panobinostat in targeting aggressive triple-negative breast cancer cell types