ISOLATION AND CHARACTERISATION OF TURNERA APHRODISIACA

India is maybe one of the biggest makers of restorative spices and is properly thought to be the "Professional flowerbed of the world". Enormous quantities of restorative spices have been in need for millennia, in some structure, under the native frameworks of medication. Detachment and depiction of Turnera aphrodisiaca leaf includes the concentrate was subjected to HPTLC for combination portrayal and separation. HPTLC stands for high-level tender loving care, and it is used to separate and identify pieces. The differentiation between each component's adsorption coefficients determines how each component is divided, and identifiable evidence rests on the correlation of Rf values. All the experiments' solvents came from MERCK and were of the high-performance liquid chromatography (HPLC) quality. The Exact XB 12A digital balance was used to take precise measurements. Using the HPTLC method, the ethanolic extract of Turnera aphrodisiaca leaf was extracted and characterized. The ethanol extract was placed as discrete bands onto silica gel 60 F254 HPTLC plates that were 10x10 cm in size together with rutin and quercetin standards.Utilizing the HPTLC technique, Turnera aphrodisiaca leaf extract was isolated and characterized. The outcomes demonstrated that rutin was present in the crude extrac

Hepatoprotective activity of ethanolic and aqueous extract of Turnera aphrodisiaca leaves against CCl4-induced liver injury in rats

Background: The botanical Latin name of the plant, Turnera aphrodisiaca, describes its ancient use as an aphrodisiac.Methods: The aim of the present study is to evaluate the protective effect of ethanolic and aqueous extract of Turnera aphrodisiaca leaves against carbon tetrachloride (CCl4)-induced liver damage in male Wistar rats.Results: Administration with ethanolic and aqueous extract of Turnera aphrodisiaca leaves (200 and 400 mg/kg) for 7 days significantly reduced the impact of CCl4 toxicity on the serum markers of liver damage, aspartate aminotransferase, alanine aminotransferase and alkaline phosphatase in a dose dependent matter. In addition, treatment of both the extracts resulted in markedly increased the levels of superoxide dismutase and catalase enzymes in rats. The histopathological studies in the liver of rats also supported that both extracts markedly reduced the toxicity of CCL4 and preserved the histoarchitecture of the liver tissue to near normal.Conclusion: Thus, the results suggest that ethanolic and aqueous extract of  Turnera aphrodisiaca leaves acts as a potent hepatoprotective agent against CCl4 induced hepatotoxicity in rats

Anticonvulsant and antioxidant effect of hydroalcoholic extract of Valeriana wallichii rhizomes in acute and chronic models of epilepsy in albino rats

585-591Oxidative stress plays an important role in the aetiology of seizure-induced neuronal death. The present study was aimed to evaluate the anticonvulsant and antioxidant activity of hydroalcoholic extract of Valeriana wallichii (HAEVW) rhizomes in albino rats. Flavonoids possess a potent anticonvulsant effect through modulation of GABA/BDZ receptor and neuroprotective effect by regulating oxidative stress in PTZ-induced convulsion. Total phenolics and flavonoids content of HAEVW rhizomes were measured and the anticonvulsant activity was evaluated using PTZ and MES induced convulsion models. The in-vivo antioxidant activity was evaluated using the PTZ-kindled model by estimating brain malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), and nitric oxide (NO). It was observed that the flavonoids content of the extract was 80% of the total phenolics content and HAEVW (200 and 400 mg/kg) showed a significant (P <0.01) delay in onset, decrease in duration of tonic convulsion and hind limb tonic extension (HLTE) in PTZ and MES induced convulsion respectively. In the PTZ-kindling model, HAEVW rhizomes (400 mg/kg) restored the MDA and all antioxidant parameters to normal except NO. It was concluded that flavonoids of rhizomes of V. wallichii may be responsible for the anticonvulsant and antioxidant activity of the extract

Anticonvulsant and antioxidant effect of hydroalcoholic extract of Valeriana wallichii rhizomes in acute and chronic models of epilepsy in albino rats

Oxidative stress plays an important role in the aetiology of seizure-induced neuronal death. The present study was aimed to evaluate the anticonvulsant and antioxidant activity of hydroalcoholic extract of Valeriana wallichii (HAEVW) rhizomes in albino rats. Flavonoids possess a potent anticonvulsant effect through modulation of GABA/BDZ receptor and neuroprotective effect by regulating oxidative stress in PTZ-induced convulsion. Total phenolics and flavonoids content of HAEVW rhizomes were measured and the anticonvulsant activity was evaluated using PTZ and MES induced convulsion models. The in-vivo antioxidant activity was evaluated using the PTZ-kindled model by estimating brain malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), and nitric oxide (NO). It was observed that the flavonoids content of the extract was 80% of the total phenolics content and HAEVW (200 and 400 mg/kg) showed a significant (P &lt;0.01) delay in onset, decrease in duration of tonic convulsion and hind limb tonic extension (HLTE) in PTZ and MES induced convulsion respectively. In the PTZ-kindling model, HAEVW rhizomes (400 mg/kg) restored the MDA and all antioxidant parameters to normal except NO. It was concluded that flavonoids of rhizomes of V. wallichii may be responsible for the anticonvulsant and antioxidant activity of the extract

Quality by Design enabled enhanced bioanalytical extraction and UFLC determination of vilazodone from rat serum

<p>An ultrafast liquid chromatographic bioanalytical method was developed and validated for the determination of vilazodone in Wistar rat serum. Principles of quality by design were implemented for enhancing the bioanalytical liquid–liquid extraction of vilazodone from rat serum. A Box–Behnken design was utilized in the studies by selecting extraction time, centrifugation speed, and vortex time as the critical method variables for evaluating their effect on the analytical attribute, i.e., %recovery of vilazodone. Chromatographic separation was achieved within a run time of 10 min using a C-18 column and mobile phase comprising of methanol:phosphate buffer of pH 7 (85:15 v/v) flowing at 1.5 mL/min. Photodiode array detection was performed at 242 nm. Results of validation studies were satisfactory. The method was linear over a concentration of 100–2,000 ng/mL with acceptable accuracy and precision. Limits of detection and quantitation for the developed method were 50 and 100 ng/mL, respectively. This QbD-based approach was found suitable for routine bioanalysis of vilazodone in the biological matrix.</p