Effect of carotenoid source and dietary lipid content on blood astaxanthin concentration in rainbow trout (Oncorhynchus mykiss)

Astaxanthin concentration in the blood of rainbow trout was studied in a feeding trial with two different astaxanthin sources: green algae Haematococcus pluvialis and commercial beadlets of 8% astaxanthin content (CAROPHYLL® Pink), and two different dietary lipid levels. The green algae contained 1.4% of carotenoids on a dry matter basis: free astaxanthin (<1%), astaxanthin monoester (24.3%); astaxanthin diester (70.2%) and lutein (4.8%). Algal biomass was mechanically ground to disrupt the cell wall before incorporation in the feed. Hydrolysis of astaxanthin esters from algae occurred during the pelletization even at a low process temperature (43°C). Rainbow trout with an initial mean body weight of 150 g were fed experimental diets supplemented at a rate of 100 mg pigment/kg diet combined with two different lipid levels (9 and 24%) during 5 days. Astaxanthin concentration in the serum ranged from 5.3 μg/ml (8.9 nmol/ml) to 9.0 μg/ml (15.1 nmol/ml). Astaxanthin concentration in the serum was higher for fish fed high lipid level diets, independently of the astaxanthin source. No differences in the astaxanthin serum concentration were found for fish fed diets supplemented with either natural or synthetic astaxanthin, respectively 9.0±1.9 and 8.4±2.4 μg astaxanthin/ml serum, when dietary lipid level was high (24%). On the other hand, there was a higher blood astaxanthin concentration in fish fed diets supplemented with algal biomass (7.0±2.4 μg astaxanthin/ml serum) compared to synthetic astaxanthin (5.3±2.0 μg astaxanthin/ml serum) when dietary lipid level was low (9%

Anoxic and aerobic values for the yield coefficient of the heterotrophic biomass: Determination at full-scale plants and consequences on simulations

The present study aims at optimising the nitrification and denitrification phases at intermittently aerated process (activated sludge) removing nitrogen from municipal wastewater. The nitrogen removal performance recorded at 22 intermittently aerated plants was compared to the results obtained from the simulations given by the widely used ASM1. It is shown that simulations with a single value for the heterotrophic yield with any electron acceptor over-predict the nitrate concentration in the effluent of treatment plants. The reduction of this coefficient by 20% for anoxic conditions reduces the nitrate concentration by 10 g N&#183;m-3. It significantly improves the accuracy of the predictions of nitrate concentrations in treatedeffluents compare to real data. Simulations with dual values (aerobic and anoxic conditions) for heterotrophic yield (modified ASM1) were then used to determine the practical daily aerobic time interval to meet a given nitrogen discharge objective. Finally, to support design decisions, the relevance of a pre-denitrification configuration in front of an intermittently aerated tank was studied. It is shown that when the load of BOD5 is below the conventional design value, a small contribution of the anoxic zone to nitrate removal occurs, except for over-aerated plants. When plants receive a higher load of BOD5, the modified ASM1 suggests that the anoxic zone has a higher contribution to nitrogen removal, for both correctly and over-aerated plants

Maximum growth and decay rates of autotrophic biomass to simulate nitrogen removal at 10°C with municipal activated sludge plants

The present study aims at determining most likely values for the maximum growth rate (μA, max) and the endogenous decay rate (bA) of nitrifiers for activated sludge processes treating municipal wastewater operated at low temperature (10°C). The work used nitrification rate data measured on 10 full-scale plants and 2 pilot plants fed with domestic sewage. This set of data was combined with a modelling and a theoretical approach. The unified values (μA, max = 0.45·d-1 and bA = 0.13·d-1) were obtained at 10°C for the kinetic parameters of the autotrophic biomass in the SRT range 10 to 50 d. In addition, the factors affecting the expected nitrification rate (rv, nit) were established by a theoretical approach and confirmed by experimental results. For a given SRT, a linear relationship with the nitrogen volumetric loading rate was shown. The COD/TKN ratio of the influent on the nitrification rate was demonstrated. Finally, an operational tool for the verification of the nitrification rate in the design procedure of activated sludge processes is proposed.Keywords: nitrification; kinetics; low temperature; autotrophic biomass, maximum growth rate; decay rat

Evolution of the ridges of Midelt-Errachidia section in the High Atlas revealed by paleomagnetic data

New paleomagnetic data (43 sites) from Mesozoic sediments are contributed in this work, verifying the presence of a pervasive syntectonic Early Cretaceous remagnetization in the easternmost area of the Moroccan High Atlas. Using the small circle intersection method, we have calculated the characteristic remagnetization direction (Dec: 337.3, Inc: 38.4) that fits with a 100-Ma age, according to the Apparent Polar Wander Path of Africa. The paleomagnetic vectors of remagnetization are used to obtain the geometry during the remagnetization stage (100Ma) of one of the most renowned geological cross sections of the High Atlas, the Midelt-Errachidia profile. The partial restoration of the cross section at 100Ma allows us to determine the dips of the beds at the remagnetization stage in five structures (ridges or anticlines). Our results indicate that the five ridges that configure the Midelt-Errachidia profile were initiated to different degrees prior to wholesale compressive deformation during the Cenozoic. This configuration can be explained according to two different scenarios that we discuss in this paper: transpression and diapirism. The geological model obtained, both at present and at 100Ma, indicates the existence of a Mesozoic cover substantially decolled from the Paleozoic basement, what strongly contrasts with previously published transects of the same area

Constraints on Energy Intake in Fish: The Link between Diet Composition, Energy Metabolism, and Energy Intake in Rainbow Trout

The hypothesis was tested that fish fed to satiation with iso-energetic diets differing in macronutrient composition will have different digestible energy intakes (DEI) but similar total heat production. Four iso-energetic diets (2×2 factorial design) were formulated having a contrast in i) the ratio of protein to energy (P/E): high (HP/E) vs. low (LP/E) and ii) the type of non-protein energy (NPE) source: fat vs. carbohydrate which were iso-energetically exchanged. Triplicate groups (35 fish/tank) of rainbow trout were hand-fed each diet twice daily to satiation for 6 weeks under non-limiting water oxygen conditions. Feed intake (FI), DEI (kJ kg−0.8 d−1) and growth (g kg−0.8 d−1) of trout were affected by the interaction between P/E ratio and NPE source of the diet (P<0.05). Regardless of dietary P/E ratio, the inclusion of carbohydrate compared to fat as main NPE source reduced DEI and growth of trout by ∼20%. The diet-induced differences in FI and DEI show that trout did not compensate for the dietary differences in digestible energy or digestible protein contents. Further, changes in body fat store and plasma glucose did not seem to exert a homeostatic feedback control on DEI. Independent of the diet composition, heat production of trout did not differ (P>0.05). Our data suggest that the control of DEI in trout might be a function of heat production, which in turn might reflect a physiological limit related with oxidative metabolism