Highly efficient optogenetic cell ablation in C. elegans using membrane-targeted miniSOG.

The genetically encoded photosensitizer miniSOG (mini Singlet Oxygen Generator) can be used to kill cells in C. elegans. miniSOG generates the reactive oxygen species (ROS) singlet oxygen after illumination with blue light. Illumination of neurons expressing miniSOG targeted to the outer mitochondrial membrane (mito-miniSOG) causes neuronal death. To enhance miniSOG's efficiency as an ablation tool in multiple cell types we tested alternative targeting signals. We find that membrane targeted miniSOG allows highly efficient cell killing. When combined with a point mutation that increases miniSOG's ROS generation, membrane targeted miniSOG can ablate neurons in less than one tenth the time of mito-miniSOG. We extend the miniSOG ablation technique to non-neuronal tissues, revealing an essential role for the epidermis in locomotion. These improvements expand the utility and throughput of optogenetic cell ablation in C. elegans

Study of controlled diffusion stator blading. 1. Aerodynamic and mechanical design report

Pratt & Whitney Aircraft is conducting a test program for NASA in order to demonstrate that a controlled-diffusion stator provides low losses at high loadings and Mach numbers. The technology has shown great promise in wind tunnel tests. Details of the design of the controlled diffusion stator vanes and the multiple-circular-arc rotor blades are presented. The stage, including stator and rotor, was designed to be suitable for the first-stage of an advanced multistage, high-pressure compressor

Do actions occur inside the body?

The paper offers a critical examination of Jennifer Hornsby's view that actions are internal to the body. It focuses on three of Hornsby's central claims: (P) many actions are bodily movements (in a special sense of the word “movement”) (Q) all actions are tryings; and (R) all actions occur inside the body. It is argued, contra Hornsby, that we may accept (P) and (Q) without accepting also the implausible (R). Two arguments are first offered in favour of the thesis (Contrary-R): that no actions occur inside the body. Three of Hornsby's arguments in favour of R are then examined. It is argued that we need to make a distinction between the causes and the causings of bodily movements (in the ordinary sense of the word “movement”) and that actions ought to be identified with the latter rather than the former. This distinction is then used to show how Hornsby's arguments for (R) may be resisted

Constraining the metallicities, ages, star formation histories, and ionizing continua of extragalactic massive star populations

We infer the properties of massive star populations using the far-ultraviolet stellar continua of 61 star-forming galaxies: 42 at low-z observed with HST and 19 at z~2 from the Megasaura sample. We fit each stellar continuum with a linear combination of up to 50 single age and single metallicity Starburst99 models. From these fits, we derive light-weighted ages and metallicities, which agree with stellar wind and photospheric spectral features, and infer the spectral shapes and strengths of the ionizing continua. Inferred light-weighted stellar metallicities span 0.05-1.5 Z$_\odot$ and are similar to the measured nebular metallicities. We quantify the ionizing continua using the ratio of the ionizing flux at 900\AA\ to the non-ionizing flux at 1500\AA\ and demonstrate the evolution of this ratio with stellar age and metallicity using theoretical single burst models. These single burst models only match the inferred ionizing continua of half of the sample, while the other half are described by a mixture of stellar ages. Mixed age populations produce stronger and harder ionizing spectra than continuous star formation histories, but, contrary to previous studies that assume constant star formation, have similar stellar and nebular metallicities. Stellar population age and metallicity affect the far-UV continua in different and distinguishable ways; assuming a constant star formation history diminishes the diagnostic power. Finally, we provide simple prescriptions to determine the ionizing photon production efficiency ($\xi_{ion}$) from the stellar population properties. $\xi_{ion}$ has a range of log($\xi_{ion})=24.4-25.7$ Hz erg$^{-1}$ that depends on stellar age, metallicity, star formation history, and contributions from binary star evolution. These stellar population properties must be observationally determined to determine the number of ionizing photons generated by massive stars.Comment: 31 pages, 23 figures, resubmitted to ApJ after incorporating the referee's comments. Comments encourage

A geometric basis for the standard-model gauge group

A geometric approach to the standard model in terms of the Clifford algebra Cl_7 is advanced. A key feature of the model is its use of an algebraic spinor for one generation of leptons and quarks. Spinor transformations separate into left-sided ("exterior") and right-sided ("interior") types. By definition, Poincare transformations are exterior ones. We consider all rotations in the seven-dimensional space that (1) conserve the spacetime components of the particle and antiparticle currents and (2) do not couple the right-chiral neutrino. These rotations comprise additional exterior transformations that commute with the Poincare group and form the group SU(2)_L, interior ones that constitute SU(3)_C, and a unique group of coupled double-sided rotations with U(1)_Y symmetry. The spinor mediates a physical coupling of Poincare and isotopic symmetries within the restrictions of the Coleman--Mandula theorem. The four extra spacelike dimensions in the model form a basis for the Higgs isodoublet field, whose symmetry requires the chirality of SU(2). The charge assignments of both the fundamental fermions and the Higgs boson are produced exactly.Comment: 17 pages, LaTeX requires iopart. Accepted for publication in J. Phys. A: Math. Gen. 9 Mar 2001. Typos correcte

Corrigendum: A Pipeline for Volume Electron Microscopy of the Caenorhabditis elegans Nervous System.

[This corrects the article DOI: 10.3389/fncir.2018.00094.]

A Pipeline for Volume Electron Microscopy of the Caenorhabditis elegans Nervous System.

The "connectome," a comprehensive wiring diagram of synaptic connectivity, is achieved through volume electron microscopy (vEM) analysis of an entire nervous system and all associated non-neuronal tissues. White et al. (1986) pioneered the fully manual reconstruction of a connectome using Caenorhabditis elegans. Recent advances in vEM allow mapping new C. elegans connectomes with increased throughput, and reduced subjectivity. Current vEM studies aim to not only fill the remaining gaps in the original connectome, but also address fundamental questions including how the connectome changes during development, the nature of individuality, sexual dimorphism, and how genetic and environmental factors regulate connectivity. Here we describe our current vEM pipeline and projected improvements for the study of the C. elegans nervous system and beyond

Intense CIII] 1907,1909 emission from a strong Lyman continuum emitting galaxy

We have obtained the first complete ultraviolet (UV) spectrum of a strong Lyman continuum(LyC) emitter at low redshift -- the compact, low-metallicity, star-forming galaxy J1154+2443 -- with a Lyman continuum escape fraction of 46% discovered recently. The Space Telescope Imaging Spectrograph spectrum shows strong Lya and CIII] 1909 emission, as well as OIII] 1666. Our observations show that strong LyC emitters can have UV emission lines with a high equivalent width (e.g. EW(CIII])$=11.7 \pm 2.9 \AA$ rest-frame), although their equivalent widths should be reduced due to the loss of ionizing photons. The intrinsic ionizing photon production efficiency of J1154+2443 is high, $\log(\xi_{\rm ion}^0)=25.56$ erg$^{-1}$ Hz, comparable to that of other recently discovered $z \sim 0.3-0.4$ LyC emitters. Combining our measurements and earlier determinations from the literature, we find a trend of increasing $\xi_{\rm ion}^0$ with increasing CIII] 1909 equivalent width, which can be understood by a combination of decreasing stellar population age and metallicity. Simple ionization and density-bounded photoionization models can explain the main observational features including the UV spectrum of J1154+2443.Comment: 5 pages, 4 figures. Accepted for publication in A&A Letter

Do galaxies that leak ionizing photons have extreme outflows?

To reionize the early universe, high-energy photons must escape the galaxies that produce them. It has been suggested that stellar feedback drives galactic outflows out of star-forming regions, creating low density channels through which ionizing photons escape into the inter-galactic medium. We compare the galactic outflow properties of confirmed Lyman continuum (LyC) leaking galaxies to a control sample of nearby star-forming galaxies to explore whether the outflows from leakers are extreme as compared to the control sample. We use data from the Cosmic Origins Spectrograph on the Hubble Space Telescope to measure the equivalent widths and velocities of Si II and Si III absorption lines, tracing neutral and ionized galactic outflows. We find that the Si II and Si III equivalent widths of the LyC leakers reside on the low-end of the trend established by the control sample. The leakers' velocities are not statistically different than the control sample, but their absorption line profiles have a different asymmetry: their central velocities are closer to their maximum velocities. The outflow kinematics and equivalent widths are consistent with the scaling relations between outflow properties and host galaxy properties -- most notably metallicity -- defined by the control sample. Additionally, we use the Ly\alpha\ profiles to show that the Si II equivalent width scales with the Ly\alpha\ peak velocity separation. We determine that the low equivalent widths of the leakers are likely driven by low metallicities and low H I column densities, consistent with a density-bounded ionization region, although we cannot rule out significant variations in covering fraction. While we do not find that the LyC leakers have extreme outflow velocities, the low maximum-to-central velocity ratios demonstrate the importance of the acceleration and density profiles for LyC and Ly\alpha\ escape. [abridged]Comment: 17 pages, 8 Figures. Accepted for publication in Astronomy & Astrophysic