524 research outputs found

    The Design and Use of a Temperature-Compensated Hot-Film Anemometer System for Boundary-Layer Flow Transition Detection on Supersonic Aircraft

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    An airborne temperature-compensated hot-film anemometer system has been designed, fabricated, and used to obtain in-flight airfoil boundary-layer flow transition data by the NASA Ames-Dryden Flight Research Facility. Salient features of the anemometer include near constant sensitivity over the full flight envelope, installation without coaxial wiring, low-noise outputs, and self-contained signal conditioning with dynamic and steady-state outputs. The small size, low-power dissipation, and modular design make the anemometer suitable for use in modern high-performance research aircraft. Design of the temperature-compensated hot-film anemometer and its use for flow transition detection on a laminar flow flight research project are described. Also presented are data gathered in flight which is representative of the temperature-compensated hot-film anemometer operation at subsonic, transonic, and supersonic flight conditions

    Development of a temperature-compensated hot-film anemometer system for boundary-layer transition detection on high-performance aircraft

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    A hot-film constant-temperature anemometer (CTA) system was flight-tested and evaluated as a candidate sensor for determining boundary-layer transition on high-performance aircraft. The hot-film gage withstood an extreme flow environment characterized by shock waves and high dynamic pressures, although sensitivity to the local total temperature with the CTA indicated the need for some form of temperature compensation. A temperature-compensation scheme was developed and two CTAs were modified and flight-tested on the F-104/Flight Test Fixture (FTF) facility at a variety of Mach numbers and altitudes, ranging from 0.4 to 1.8 and 5,000 to 40,000 ft respectively

    Identification of the subcellular location of the a-2,3-sialyltransferase of the bacterial pathogen Neisseria gonorrhoeae

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    Techniques used in the F-14 variable-sweep transition flight experiment

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    This paper discusses and evaluates the test measurement techniques used to determine the laminar-to-turbulent boundary layer transition location in the F-14 variable-sweep transition flight experiment (VSTFE). The main objective of the VSTFE was to determine the effects of wing sweep on the laminar-to-turbulent transition location at conditions representative of transport aircraft. Four methods were used to determine the transition location: (1) a hot-film anemometer system; (2) two boundary-layer rakes; (3) surface pitot tubes; and (4) liquid crystals for flow visualization. Of the four methods, the hot-film anemometer system was the most reliable indicator of transition

    Oral History Interview: Fred R. Chiles Jr.

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    This interview is one of series conducted concerning the Oral History of Appalachia. Mr. Chiles was the director of the Arts Camp at Camp Washington Carver. He talks at length about his childhood years, his years as a 4H camp member, what caused him to become interested in the camp as an adult, and their activities. He talks of the different groups that utilized the camp, such as the Girl Scouts and Brownie Troupes. He discusses camp rules, the fact that the swimming pool at Camp Carver was the first and for some time the only swimming pool for blacks, and the NAACP and their involvement. He also talks about his segregation experiences, George Washington Carver, Booker T. Washington, and Ralph Bunche.https://mds.marshall.edu/oral_history/1500/thumbnail.jp

    RNAi Trigger Delivery into Anopheles gambiae Pupae

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    Citation: Regna, K., Harrison, R. M., Heyse, S. A., Chiles, T. C., Michel, K., & Muskavitch, M. A. T. (2016). RNAi Trigger Delivery into Anopheles gambiae Pupae. Jove-Journal of Visualized Experiments(109), 9. doi:10.3791/53738RNA interference (RNAi), a naturally occurring phenomenon in eukaryotic organisms, is an extremely valuable tool that can be utilized in the laboratory for functional genomic studies. The ability to knockdown individual genes selectively via this reverse genetic technique has allowed many researchers to rapidly uncover the biological roles of numerous genes within many organisms, by evaluation of loss-of-function phenotypes. In the major human malaria vector Anopheles gambiae, the predominant method used to reduce the function of targeted genes involves injection of double-stranded (dsRNA) into the hemocoel of the adult mosquito. While this method has been successful, gene knockdown in adults excludes the functional assessment of genes that are expressed and potentially play roles during pre-adult stages, as well as genes that are expressed in limited numbers of cells in adult mosquitoes. We describe a method for the injection of Serine Protease Inhibitor 2 (SRPN2) dsRNA during the early pupal stage and validate SRPN2 protein knockdown by observing decreased target protein levels and the formation of melanotic pseudo-tumors in SRPN2 knockdown adult mosquitoes. This evident phenotype has been described previously for adult stage knockdown of SRPN2 function, and we have recapitulated this adult phenotype by SRPN2 knockdown initiated during pupal development. When used in conjunction with a dye-labeled dsRNA solution, this technique enables easy visualization by simple light microscopy of injection quality and distribution of dsRNA in the hemocoel
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