Thermal liquid biopsy for monitoring melanoma patients under surveillance during treatment: A pilot study

Background: Differential Scanning Calorimetry (DSC) is a technique traditionally used to study thermally induced macromolecular transitions, and it has recently been proposed as a novel approach for diagnosis and monitoring of several diseases. We report a pilot study applying Thermal Liquid Biopsy (TLB, DSC thermograms of plasma samples) as a new clinical approach for diagnostic assessment of melanoma patients. Methods: Multiparametric analysis of DSC thermograms of patient plasma samples collected during treatment and surveillance (63 samples from 10 patients) were compared with clinical and diagnostic imaging assessment to determine the utility of thermograms for diagnostic assessment in melanoma. Nine of the ten patients were stage 2 or 3 melanoma subjects receiving adjuvant therapy after surgical resection of their melanomas. The other patient had unresectable stage 4 melanoma and was treated with immunotherapy. Two reference groups were used: (A) 36 healthy subjects and (B) 13 samples from 8 melanoma patients who had completed successful surgical management of their disease and were determined by continued clinical assessment to have no evidence of disease. Results: Plasma thermogram analysis applied to melanoma patients generally agrees with clinical evaluation determined by physical assessment or diagnostic imaging (~80% agreement). No false negatives were obtained from DSC thermograms. Importantly, this methodology was able to detect changes in disease status before it was identified clinically. Conclusions: Thermal Liquid Biopsy could be used in combination with current clinical assessment for the earlier detection of melanoma recurrence and metastasis. General significance: TLB offers advantages over current diagnostic techniques (PET/CT imaging), limited in frequency by radiation burden and expense, in providing a minimally-invasive, low-risk, low-cost clinical test for more frequent personalized patient monitoring to assess recurrence and facilitate clinical decision-making.Peer Reviewe

They are all 'doing gender' but are they are all passing? A case study of the appropriation of a sociological concept

The concept of 'doing gender' was placed on the sociological agenda by West and Zimmerman. In their seminal paper published in 1987, they provided a systematic theory of gender as a routine and ongoing process and outlined a distinctly ethnomethodological approach to investigating how gender is enacted, understood and rendered accountable. West and Zimmerman's notion of 'doing gender' has subsequently become a central concept in many fields of sociological research, however, upon closer examination although many authors claim to be using the concept - in effect to be doing 'doing gender' - the concept's intellectual roots in ethnomethodology are not always recognised or reflected: in short not all are passing. The purpose of our study is to explore the career trajectory of this concept and to systematically assess the manner in which 'doing' has been employed. From a review of 226 journal articles, books, dissertations and association papers, we provide an overview of the uses of this construct and examine the ways in which 'doing gender' has been assimilated into current theoretical and methodological practice