George F. Cheney collection, 1954-1955

Letter, typed summary of interview (4 leaves) with Leonard Sackett with follow-up questions and answers relating to the March-Spaulding Farm near Warren, Minn., farming in the Warren, Minn. area, and some family history material

The question of organization: A manifesto for alternatives

This paper is an attempt to articulate some general principles which might guide anarchist thinking about organized alternatives to market managerialism and might be read as a sort of manifesto for defining ‘the alternative’. That is to say, it describes what we include in our list of useful possibilities, and what to exclude on the grounds that it doesn’t fit with our definition of what counts as sufficiently different from the present. We suggest three principles which we believe that radicals should be guided by – autonomy, solidarity and responsibility – and that we think any reflection on the politics of organizing needs to deal with. We wish to encourage forms of organizing which respect personal autonomy, but within a framework of co-operation, and are attentive to the sorts of futures which they will produce. This is a simple statement to make but it produces some complex outcomes since gaining agreement on any of these ideas is not a simple matter

The question of organization: A manifesto for alternatives * *

abstract This paper is an attempt to articulate some general principles which might guide anarchist thinking about organized alternatives to market managerialism and might be read as a sort of manifesto for defining 'the alternative'. That is to say, it describes what we include in our list of useful possibilities, and what to exclude on the grounds that it doesn't fit with our definition of what counts as sufficiently different from the present. We suggest three principles which we believe that radicals should be guided byautonomy, solidarity and responsibility -and that we think any reflection on the politics of organizing needs to deal with. We wish to encourage forms of organizing which respect personal autonomy, but within a framework of co-operation, and are attentive to the sorts of futures which they will produce. This is a simple statement to make but it produces some complex outcomes since gaining agreement on any of these ideas is not a simple matter

State of the art on measuring driver state and technology-based risk prevention and mitigation Findings from the i-DREAMS project

Advanced vehicle automation and the incorporation of more digital technologies in the task of driving, bring about new challenges in terms of the operator/vehicle/environment framework, where human factors play a crucial role. This paper attempts to consolidate the state-of-the-art in driver state measuring, as well as the corresponding technologies for risk assessment and mitigation, as part of the i-DREAMS project. Initially, the critical indicators for driver profiling with regards to safety risk are identified and the most prominent task complexity indicators are established. This is followed by linking the aforementioned indicators with efficient technologies for real-time measuring and risk assessment and finally a brief overview of interventions modules is outlined in order to prevent and mitigate collision risk. The results of this review will provide an overall multimodal set of factors and technologies for driver monitoring and risk mitigation, essential for road safety researchers and practitioners worldwide<br

Myosin Vb Is Required for Trafficking of the Cystic Fibrosis Transmembrane Conductance Regulator in Rab11a-specific Apical Recycling Endosomes in Polarized Human Airway Epithelial Cells

Cystic fibrosis transmembrane conductance regulator (CFTR)-mediated Cl(-) secretion across fluid-transporting epithelia is regulated, in part, by modulating the number of CFTR Cl(-) channels in the plasma membrane by adjusting CFTR endocytosis and recycling. However, the mechanisms that regulate CFTR recycling in airway epithelial cells remain unknown, at least in part, because the recycling itineraries of CFTR in these cells are incompletely understood. In a previous study, we demonstrated that CFTR undergoes trafficking in Rab11a-specific apical recycling endosomes in human airway epithelial cells. Myosin Vb is a plus-end-directed, actin-based mechanoenzyme that facilitates protein trafficking in Rab11a-specific recycling vesicles in several cell model systems. There are no published studies examining the role of myosin Vb in airway epithelial cells. Thus, the goal of this study was to determine whether myosin Vb facilitates CFTR recycling in polarized human airway epithelial cells. Endogenous CFTR formed a complex with endogenous myosin Vb and Rab11a. Silencing myosin Vb by RNA-mediated interference decreased the expression of wild-type CFTR and DeltaF508-CFTR in the apical membrane and decreased CFTR-mediated Cl(-) secretion across polarized human airway epithelial cells. A recombinant tail domain fragment of myosin Vb attenuated the plasma membrane expression of CFTR by arresting CFTR recycling. The dominant-negative effect was dependent on the ability of the myosin Vb tail fragment to interact with Rab11a. Taken together, these data indicate that myosin Vb is required for CFTR recycling in Rab11a-specific apical recycling endosomes in polarized human airway epithelial cells

Do Dispersing Monkeys Follow Kin? Evidence from Gray-cheeked Mangabeys (Lophocebus albigena)

Among social vertebrates, immigrants may incur a substantial fitness cost when they attempt to join a new group. Dispersers could reduce that cost, or increase their probability of mating via coalition formation, by immigrating into groups containing first- or second-degree relatives. We here examine whether dispersing males tend to move into groups containing fathers or brothers in gray-cheeked mangabeys (Lophocebus albigena) in Kibale National Park, Uganda. We sampled blood from 21 subadult and adult male mangabeys in 7 social groups and genotyped them at 17 microsatellite loci. Twelve genotyped males dispersed to groups containing other genotyped adult males during the study; in only 1 case did the group contain a probable male relative. Contrary to the prediction that dispersing males would follow kin, relatively few adult male dyads were likely first- or second-degree relatives; opportunities for kin-biased dispersal by mangabeys appear to be rare. During 4 yr of observation, adult brothers shared a group only once, and for only 6 wk. Mean relatedness among adult males sharing a group was lower than that among males in different groups. Randomization tests indicate that closely related males share groups no more often than expected by chance, although these tests had limited power. We suggest that the demographic conditions that allow kin-biased dispersal to evolve do not occur in mangabeys, may be unusual among primates, and are worth further attention

Pathogenic copy number variants and SCN1A mutations in patients with intellectual disability and childhood-onset epilepsy

Background Copy number variants (CNVs) have been linked to neurodevelopmental disorders such as intellectual disability (ID), autism, epilepsy and psychiatric disease. There are few studies of CNVs in patients with both ID and epilepsy. Methods We evaluated the range of rare CNVs found in 80 Welsh patients with ID or developmental delay (DD), and childhood-onset epilepsy. We performed molecular cytogenetic testing by single nucleotide polymorphism array or microarray-based comparative genome hybridisation. Results 8.8 % (7/80) of the patients had at least one rare CNVs that was considered to be pathogenic or likely pathogenic. The CNVs involved known disease genes (EHMT1, MBD5 and SCN1A) and imbalances in genomic regions associated with neurodevelopmental disorders (16p11.2, 16p13.11 and 2q13). Prompted by the observation of two deletions disrupting SCN1A we undertook further testing of this gene in selected patients. This led to the identification of four pathogenic SCN1A mutations in our cohort. Conclusions We identified five rare de novo deletions and confirmed the clinical utility of array analysis in patients with ID/DD and childhood-onset epilepsy. This report adds to our clinical understanding of these rare genomic disorders and highlights SCN1A mutations as a cause of ID and epilepsy, which can easily be overlooked in adults