Some physiological studies of Fusobacterium nucleatum

Hypothesizes that differences in disease-association between Fusobacterium nucleatum biotypes may be related to differences in their physiology and metabolism. No obvious physiological differences were found that might account for suggested differences in disease association between the strains.Thesis (M.Sc.Dent.) -- University of Adelaide, Dept. of Dentistry, 199

Utilizing metabolomics and network analysis to explore the effects of artificial production methods on the chemical composition and activity of agarwood

Introduction: Agarwood is a traditional aromatic southern medicine. It has a long history of being used in traditional Chinese aromatherapy to treat insomnia, anxiety and depression. Due to the scarcity of wild resources, people have planted trees successfully and begun to explore various agarwood-inducing techniques. This study comparative analysis of volatile metabolites in agarwood produced by various inducing techniques and its potential sleep-promoting, anti-anxiety and anti-depressant network pharmacological activities. Methods: A total of 23 batches of two types of agarwood were collected, one of which was produced by artificial techniques, including 6 batches of TongTi (TT) agarwood produced by “Agar-Wit” and 6 batches of HuoLao (HL) agarwood produced by “burning, chisel and drilling”, while the other was collected from the wild, including 6 batches of BanTou (BT) agarwood with trunks broken due to natural or man-made factors and 5 batches of ChongLou (CL) agarwood with trunks damaged by moth worms. The study employed metabolomics combined with network analysis to compare the differences in volatile metabolites of agarwood produced by four commonly used inducing techniques, and explored their potential roles and possible action targets in promoting sleep, reducing anxiety, and alleviating depression. Results: A total of 147 volatile metabolites were detected in agarwood samples, mainly including small aromatic hydrocarbons, sesquiterpenes and 2-(2-phenylethyl) chromone and their pyrolysis products. The results showed composition of metabolites was minimally influenced by the agarwood induction method. However, their concentrations exhibited significant variations, with 17 metabolites showing major differences. The two most distinct metabolites were 6-methoxy-2-(2-phenylethyl) chromone and 6,7-dimethoxy-2-(2-phenylethyl) chromone. Among the volatile metabolites, 142 showed promising potential in treating insomnia, anxiety, and depression, implicating various biological and signaling pathways, predominantly ALB and TNF targets. The top three active metabolites identified were 2-(2-phenylethyl) chromone, 1,5-diphenylpent-1-en-3-one, and 6-methoxy-2-[2-(4'-methoxyphenyl) ethyl] chromone, with their relative content in the four types of agarwood being TT>HL>CL>BT. Conclusion: The differences in the content of 2-(2-phenylethyl) chromones suggest that they may be responsible for the varying therapeutic activities observed in different types of agarwood aromatherapy. This study offers theoretical support for the selection of agarwood in aromatherapy practices

The Screening of the Protective Antigens of Aeromonas hydrophila Using the Reverse Vaccinology Approach: Potential Candidates for Subunit Vaccine Development

The threat of bacterial septicemia caused by Aeromonas hydrophila infection to aquaculture growth can be prevented through vaccination, but differences among A. hydrophila strains may affect the effectiveness of non-conserved subunit vaccines or non-inactivated A. hydrophila vaccines, making the identification and development of conserved antigens crucial. In this study, a bioinformatics analysis of 4268 protein sequences encoded by the A. hydrophila J-1 strain whole genome was performed based on reverse vaccinology. The specific analysis included signal peptide prediction, transmembrane helical structure prediction, subcellular localization prediction, and antigenicity and adhesion evaluation, as well as interspecific and intraspecific homology comparison, thereby screening the 39 conserved proteins as candidate antigens for A. hydrophila vaccine. The 9 isolated A. hydrophila strains from diseased fish were categorized into 6 different molecular subtypes via enterobacterial repetitive intergenic consensus (ERIC)-PCR technology, and the coding regions of 39 identified candidate proteins were amplified via PCR and sequenced to verify their conservation in different subtypes of A. hydrophila and other Aeromonas species. In this way, conserved proteins were screened out according to the comparison results. Briefly, 16 proteins were highly conserved in different A. hydrophila subtypes, of which 2 proteins were highly conserved in Aeromonas species, which could be selected as candidate antigens for vaccines development, including type IV pilus secretin PilQ (AJE35401.1) and TolC family outer membrane protein (AJE35877.1). The present study screened the conserved antigens of A. hydrophila by using reverse vaccinology, which provided basic foundations for developing broad-spectrum protective vaccines of A. hydrophila

Optimal Hybrid Beamforming Design for Millimeter-Wave Massive Multi-User MIMO Relay Systems

As a promising technology in the next generation mobile network, millimeter-wave (mmWave) communication can mitigate the spectrum crunch of improving the network capacity by exploiting the large underutilized spectrum bands of the mmWave frequencies. The hybrid (analog/digital) beamforming of multi-data streams are widely used to further the spectrum efficiency of mmWave relay system when faced with the complex environment or long distance communication. This paper investigates the hybrid beamforming scheme for the decode-and-forward (DF) mmWave massive multiple-input multiple-output (MIMO) relay system with mixed structure and full-connected structure. We optimize hybrid beamforming of relay system by maximizing the sum rate of the overall system as an objective function. To reduce the computational complexity, we reformulate the original problem as two single-hop mmWave MIMO sum-rate maximization subproblems. Then, the piecewise successive approximation method is proposed based on the criterion which jointly designs the analog and digital beamforming stages by trying to avoid the loss of information at each stage. The hybrid beamforming of the two subproblems can be solved by the proposed scheme united with the idea of successive interference cancelation (SIC), the baseband block diagonalization (BD) scheme, and waterfilling power allocation method. Finally, simulation results confirm that the proposed optimal method can achieve good performance in hybrid beamforming design of relay system with both mixed and full-connected structures

Fractional creep model and experimental study of unsaturated silty clay in Fuyang

Due to the long-term overdraft of groundwater in Fuyang, silty clay in this area has been in an unsaturated state for a long time, which caused ground subsidence and threatened the safety of engineering construction. Creep is an important part of ground subsidence, but it is easily ignored in ground subsidence caused by groundwater overdraft. Therefore, in order to explore the creep behavior in this environment, a series of triaxial creep tests of unsaturated soil were conducted to research the effects of deviator stress, matrix suction and net confining pressure on creep. Then, based on analysis results of the geometric characteristics of the test creep curves, an improved fractional order Nishihara model of unsaturated soil under triaxial stress conditions was constructed by using fractional calculus theory. Finally, the effectiveness of improved fractional order Nishihara model was verified based on cooperation search algorithm and minimum mean square error principle. The simulation results show that the fitting curves of improved fractional order Nishihara model are in good agreement with the test curves, and it is feasible and effective to describe the creep characteristics of unsaturated silty clay in Fuyang

Near-Optimal Design for Hybrid Beamforming in mmWave Massive Multi-User MIMO Systems

Millimeter wave (mmWave) massive multiple-input multiple-output (MIMO) systems can obtain sufficient beamforming gains to combat severe path loss in signal propagation. The hybrid (analog/digital) beamforming with multiple data streams can be utilized to further improve mmWave spectral efficiency. In this paper, we focus on the hybrid beamforming design of a downlink mmWave massive multi-user MIMO (MU-MIMO) system based on full-connected structure, and aim to maximize the sum rate of the overall system as an objective function. In the analog beamforming stage, a piecewise successive iterative approximation (PSIA) algorithm is proposed to design the analog beamformer and combiner. This algorithm not only has a linear property, but also can obtain closed-form solutions. In the digital beamforming stage, the piecewise successive approximation method is utilized to design the digital beamforming based on the criterion to avoid the loss of information, which can help reduce the computational complexity and is also implemented simply. The results show that the proposed scheme achieves good sum-rate performance in the mmWave massive MU-MIMO system, and outperforms the state-of-the art MIMO hybrid beamforming design schemes, even when the number of base station antennas is not very large

Hybrid beamforming with sub-connected structure for MmWave massive multi-user MIMO relay systems

In this paper, we investigate the hybrid beamforming design with sub-connected structure for millimeter-wave (mmWave) massive multi-user multiple-input multiple-output (MU-MIMO) amplify-and-forward (AF) relay systems. Considering the constant-modulus and block-diagonal constraints, an iterative algorithm is proposed to sequentially design the analog beamforming of the base station, relay, and users. Next, the relay baseband combiner is designed by transforming the highly complicated non-convex mutual information maximization problem into an easily tractable weighted minimum mean squared error one. To mitigate the inter-user interference, a successive serial interference cancellation based and a piecewise successive approximation based methods are developed for the uniform sparse distribution and dense distribution scenarios, respectively. Simulation results demonstrate that the two proposed hybrid beamforming schemes can achieve good performance in terms of sum-rate and energy efficiency, and outperform benchmarks significantly. In addition, the two proposed schemes are shown to be robust to imperfect channel state information (CSI), even though they are studied based on perfect CSI

Diffusion-weighted imaging lesions after endovascular treatment of cerebral aneurysms: A network meta-analysis

BackgroundThromboembolism is one of the common complications in endovascular treatments including coiling alone, stent-assisted coiling (SAC), balloon-assisted coiling (BAC), and flow-diverting (FD) stents. Such treatments are widely used in intracranial aneurysms (IAs), which usually present as positive lesions in diffusion-weighted imaging (DWI). Whether these adjunctive techniques increase postprocedural DWI-positive lesions after endovascular treatment remains unclear.MethodsA thorough electronic search for the literature published in English between January 2000 and October 2022 was conducted on PubMed, Medline, and EMBASE. Eighteen studies (3 cohort studies and 15 case–control studies) involving 1,843 patients with unruptured IAs (UIAs) were included. We performed a frequentist framework network meta-analysis (NMA) to compare the rank risks of cerebral thromboembolism of the above four endovascular treatments. The incoherence test was used to analyze the statistical disagreement between direct and indirect evidence. Funnel plots were used to analyze publication bias.ResultsThe incidences of DWI lesions in patients who received FD stents, SAC, BAC, and coiling alone were 66.1% (109/165), 37.6% (299/795), 31.1% (236/759), and 25.6% (236/921). The incidence of DWI lesions in patients who received FD stents was higher than that in patients who received SAC [OR: 2.40; 95% CI (1.15, 5.00), P < 0.05], BAC [OR: 2.62; 95% CI (1.19, 5.77), P < 0.05], or coiling alone [OR: 2.77; 95% CI (1.26, 6.07), P < 0.05]. The incoherence test showed preferable consistency in this NMA. No obvious publication bias was found in the funnel plot.ConclusionFD stent placement brings more ischemic lesions identified by DWI than any other procedures for patients with UIA. The characteristics of FD stents may result in a high incidence of DWI lesions

Controlling bacteriophage phi29 DNA-packaging motor by addition or discharge of a peptide at N-terminus of connector protein that interacts with pRNA

Bacteriophage phi29 utilizes a motor to translocate genomic DNA into a preformed procapsid. The motor contains six pRNAs, an enzyme and one 12-subunit connector with a central channel for DNA transportation. A 20-residue peptide containing a His-tag was fused to the N-terminus of the connector protein gp10. This fusion neither interfered with procapsid assembly nor affected the morphology of the prolate-shaped procapsid. However, the pRNA binding and virion assembly activity were greatly reduced. Such decreased functions can be switched back on by the removal of the tag via protease cleavage, supporting the previous finding that the N-terminus of gp10 is essential for the pRNA binding. The DNA-packaging efficiency with dimeric pRNA was more seriously affected by the extension than with monomeric pRNA. It is speculated that the fusion of the tag generated physical hindrance to pRNA binding, with greater influence for the dimers than the monomers due to their size. These results reveal a potential to turn off and turn on the motor by attaching or removing, respectively, a component to outer part of the motor, and offers an approach for the inhibition of viral replication by using a drug or a small peptide targeted to motor components

GLUT3 expression in cystic change induced by hypoxia in pituitary adenomas

Tumor cells require large amounts of energy to sustain growth. Through the mediated transport of glucose transporters, the uptake and utilization of glucose by tumor cells are significantly enhanced in the hypoxic microenvironment. Pituitary adenomas are benign tumors with high-energy metabolisms. We aimed to investigate the role of expression of glucose transporter 3 (GLUT3) and glucose transporter 1 (GLUT1) in pituitary adenomas, including effects on size, cystic change and hormone type. Pituitary adenomas from 203 patients were collected from January 2013 to April 2017, and immunohistochemical analysis was used to detect the expression of GLUT3 and GLUT1 in tumor specimens. GLUT3-positive expression in the cystic change group was higher than that in the non-cystic change group (P = 0.018). Proportions of GLUT3-positive staining of microadenomas, macroadenomas, and giant adenomas were 22.7 (5/22), 50.4 (66/131) and 54.0% (27/50), respectively (P = 0.022). In cases of prolactin adenoma, GLUT3-positive staining was predominant in cell membranes (P = 0.000006), while in cases of follicle-stimulating hormone or luteotropic hormone adenoma, we found mainly paranuclear dot-like GLUT3 staining (P = 0.025). In other hormonal adenomas, GLUT3 was only partially expressed, and the intensity of cell membrane or paranuclear punctate staining was weak. In contrast to GLUT3, GLUT1 expression was not associated with pituitary adenomas. Thus, our results indicate that the expression of GLUT3 in pituitary adenomas is closely related to cystic change and hormonal type. This study is the first to report a unique paranuclear dot-like GLUT3 staining pattern in pituitary adenomas